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The map label for this gene is dnaG [H]

Identifier: 134301934

GI number: 134301934

Start: 909803

End: 911620

Strand: Reverse

Name: dnaG [H]

Synonym: FTW_0945

Alternate gene names: 134301934

Gene position: 911620-909803 (Counterclockwise)

Preceding gene: 134301935

Following gene: 134301933

Centisome position: 48.02

GC content: 31.08

Gene sequence:

>1818_bases
ATGGCGAAAAAAGTCTCGAATAGTTTTATAAAAGAGCTTGTAGCTACAGCTGATATCGTTGATGTTGTTTCAAGATATGT
TAACTTGAAAAAAACAGGCAAAAACTATAAAGGTTGTTGTCCTTTTCATAATGAAAAAACACCATCATTTTTTGTTAATC
CAGAGAAAAATTTTTATCATTGTTTTGGCTGTCAGGCATCTGGAGATGCATTAACTTTTGTTAAAAATATTAATAAACTT
GAGTTTATTGATGCTGTAAAAAACCTTGCTGAAATAGTTGGTAAGCCAGTTGAATATGAAAATTATTCTCAAGAAGATAT
CCAAAAAGAACAGCTATACAACAAATGTATAAGCTTTTTAGCAGCTGCTCAAAAGTATTATCGTTGGAATTTGGGTAACT
CTGTCACCAAAGATAAGGCTATAAACTATCTTAAAAAAAGAGGTATAGATAGTAGTTTGGCTAAATTCTTTGGTATAGGA
TATTCATCCGAAGGTTGGAATAATATTACTGAACTAGCAAAGTCTATAAATATTCCAGAAGAAATATTAGTTGATACTGG
GCTTGCTATAAAAAATGATAAGGGCAATTTATATGATCGTTTTCGCGGTCGTGTTATGTTCCCTATTAGAAATATTCAAG
GCAATGTAATTGCTTATGGCGGTAGAGTAACAGAGGACTCTGATGGAGTTAAGTATATAAATTCACCAGAAACTTTAGTC
TTTCAGAAAAATAATATTTTATATGGACTCTATGAGTACCGTGAAAGAAAAAAACAATATCCAGATTTAATAAATCAAAG
CCTTGTAGTAGTTGAAGGTTATATGGATGTGGTTGGTTTAGCACAGCATGGTTTTTATGCTGCAGTAGCTACATTAGGCA
CCGCATTCTCACCAAATCACGCTAAGATTTTATTTCGTGAAACTAGCTCAGTAATTTTGTGTTTTGATGGTGATGAAGCA
GGGCAAAAAGCAGCACTTAGAACTATAAAGATTTTGTTACCAATGCTAGATGGTAATAAAAAACTAAAAATTTTAACTCT
TCCAGATAAGGATGATCCTGATGATTATATTAAGAAATATGGTTTAGAAAGATTTCTCACTGCTTTAGATAATTCTCTAG
CTGTAGCAGATTTTGTAATTGATAATCTTATTCAAGGTAAGGATCTGCGTAAAGCAGAAGCAAAAGCGGAAGTTTTAGAG
AATTTAAAAAATTTTTTAGCAGATGTAGAAGATAATATTTATTCAGAAAGTATTACTGCTACTATAGCTGATAAAATTGG
TATAAAAGTTGAGCAATTTAAGAATCTACTTAAGATACGTAAGCAAACTACACTAAACGTAAATAGACAGCAAAATATTC
AGCAAAAGAAACTAGCGAAGAATCTATTGCTTGAAGAGTTTGTCCTTGCGGAATTATTTGTTAATATCGCGGATTTTCGC
ATTTTACAACATACAAACGATTTTGAAATATTTGCTACTTCGAAAAATCTTGATATCTTAGCAAAAAGCTTGAAAATTTT
AAAAGAAGACTCATCTAATCAAATTGAAGCTGTTATACTTATACAGCTATTGGCAGAGGACTATCCTGACTATAGAGAGT
ATTTTTTTGAGTTGTTAAGTTATGGTATTCGCAATACTCAAAAAAAATATGCTGAAGACAAATATCAAGAACAAATGTTC
GTTATGCTAAAAAGAGTAGAAAACTCTAGTGTAAAAAAAAGATTAAAATATTTAGGTTCATTGCCCTTTAGGTCGGATGT
CCAGGAAATGGAGCGTAAGTATTTAGTAGCAAAATTAGGCAACAGTAATATAATATAA

Upstream 100 bases:

>100_bases
TGGCAAGTGTAAAAAAAGAACTTGCTGGTAGGACAGATATGAGCAAAGTAAGCGCTATTGTGAAATCTAACTTAGCTTAA
AGGTTGAAGCTTTAATCAGT

Downstream 100 bases:

>100_bases
AATTCTATTGGAGTACTAGATGACAAAAGAAGATTTGCTCTCTGATTTAAAAGATTTGATGATTGATGGTAGGGAAAGAG
GATATTTAACTAGAGCCGAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MAKKVSNSFIKELVATADIVDVVSRYVNLKKTGKNYKGCCPFHNEKTPSFFVNPEKNFYHCFGCQASGDALTFVKNINKL
EFIDAVKNLAEIVGKPVEYENYSQEDIQKEQLYNKCISFLAAAQKYYRWNLGNSVTKDKAINYLKKRGIDSSLAKFFGIG
YSSEGWNNITELAKSINIPEEILVDTGLAIKNDKGNLYDRFRGRVMFPIRNIQGNVIAYGGRVTEDSDGVKYINSPETLV
FQKNNILYGLYEYRERKKQYPDLINQSLVVVEGYMDVVGLAQHGFYAAVATLGTAFSPNHAKILFRETSSVILCFDGDEA
GQKAALRTIKILLPMLDGNKKLKILTLPDKDDPDDYIKKYGLERFLTALDNSLAVADFVIDNLIQGKDLRKAEAKAEVLE
NLKNFLADVEDNIYSESITATIADKIGIKVEQFKNLLKIRKQTTLNVNRQQNIQQKKLAKNLLLEEFVLAELFVNIADFR
ILQHTNDFEIFATSKNLDILAKSLKILKEDSSNQIEAVILIQLLAEDYPDYREYFFELLSYGIRNTQKKYAEDKYQEQMF
VMLKRVENSSVKKRLKYLGSLPFRSDVQEMERKYLVAKLGNSNII

Sequences:

>Translated_605_residues
MAKKVSNSFIKELVATADIVDVVSRYVNLKKTGKNYKGCCPFHNEKTPSFFVNPEKNFYHCFGCQASGDALTFVKNINKL
EFIDAVKNLAEIVGKPVEYENYSQEDIQKEQLYNKCISFLAAAQKYYRWNLGNSVTKDKAINYLKKRGIDSSLAKFFGIG
YSSEGWNNITELAKSINIPEEILVDTGLAIKNDKGNLYDRFRGRVMFPIRNIQGNVIAYGGRVTEDSDGVKYINSPETLV
FQKNNILYGLYEYRERKKQYPDLINQSLVVVEGYMDVVGLAQHGFYAAVATLGTAFSPNHAKILFRETSSVILCFDGDEA
GQKAALRTIKILLPMLDGNKKLKILTLPDKDDPDDYIKKYGLERFLTALDNSLAVADFVIDNLIQGKDLRKAEAKAEVLE
NLKNFLADVEDNIYSESITATIADKIGIKVEQFKNLLKIRKQTTLNVNRQQNIQQKKLAKNLLLEEFVLAELFVNIADFR
ILQHTNDFEIFATSKNLDILAKSLKILKEDSSNQIEAVILIQLLAEDYPDYREYFFELLSYGIRNTQKKYAEDKYQEQMF
VMLKRVENSSVKKRLKYLGSLPFRSDVQEMERKYLVAKLGNSNII
>Mature_604_residues
AKKVSNSFIKELVATADIVDVVSRYVNLKKTGKNYKGCCPFHNEKTPSFFVNPEKNFYHCFGCQASGDALTFVKNINKLE
FIDAVKNLAEIVGKPVEYENYSQEDIQKEQLYNKCISFLAAAQKYYRWNLGNSVTKDKAINYLKKRGIDSSLAKFFGIGY
SSEGWNNITELAKSINIPEEILVDTGLAIKNDKGNLYDRFRGRVMFPIRNIQGNVIAYGGRVTEDSDGVKYINSPETLVF
QKNNILYGLYEYRERKKQYPDLINQSLVVVEGYMDVVGLAQHGFYAAVATLGTAFSPNHAKILFRETSSVILCFDGDEAG
QKAALRTIKILLPMLDGNKKLKILTLPDKDDPDDYIKKYGLERFLTALDNSLAVADFVIDNLIQGKDLRKAEAKAEVLEN
LKNFLADVEDNIYSESITATIADKIGIKVEQFKNLLKIRKQTTLNVNRQQNIQQKKLAKNLLLEEFVLAELFVNIADFRI
LQHTNDFEIFATSKNLDILAKSLKILKEDSSNQIEAVILIQLLAEDYPDYREYFFELLSYGIRNTQKKYAEDKYQEQMFV
MLKRVENSSVKKRLKYLGSLPFRSDVQEMERKYLVAKLGNSNII

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=434, Percent_Identity=40.5529953917051, Blast_Score=338, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 69052; Mature: 68921

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVSNSFIKELVATADIVDVVSRYVNLKKTGKNYKGCCPFHNEKTPSFFVNPEKNFYH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEE
CFGCQASGDALTFVKNINKLEFIDAVKNLAEIVGKPVEYENYSQEDIQKEQLYNKCISFL
EECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
AAAQKYYRWNLGNSVTKDKAINYLKKRGIDSSLAKFFGIGYSSEGWNNITELAKSINIPE
HHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCH
EILVDTGLAIKNDKGNLYDRFRGRVMFPIRNIQGNVIAYGGRVTEDSDGVKYINSPETLV
HHHHHCCCEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCEECCCCCCCEEECCCCEEE
FQKNNILYGLYEYRERKKQYPDLINQSLVVVEGYMDVVGLAQHGFYAAVATLGTAFSPNH
EECCCEEEHHHHHHHHHHHCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AKILFRETSSVILCFDGDEAGQKAALRTIKILLPMLDGNKKLKILTLPDKDDPDDYIKKY
CEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
GLERFLTALDNSLAVADFVIDNLIQGKDLRKAEAKAEVLENLKNFLADVEDNIYSESITA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIADKIGIKVEQFKNLLKIRKQTTLNVNRQQNIQQKKLAKNLLLEEFVLAELFVNIADFR
HHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILQHTNDFEIFATSKNLDILAKSLKILKEDSSNQIEAVILIQLLAEDYPDYREYFFELLS
HEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YGIRNTQKKYAEDKYQEQMFVMLKRVENSSVKKRLKYLGSLPFRSDVQEMERKYLVAKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
NSNII
CCCCC
>Mature Secondary Structure 
AKKVSNSFIKELVATADIVDVVSRYVNLKKTGKNYKGCCPFHNEKTPSFFVNPEKNFYH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEE
CFGCQASGDALTFVKNINKLEFIDAVKNLAEIVGKPVEYENYSQEDIQKEQLYNKCISFL
EECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
AAAQKYYRWNLGNSVTKDKAINYLKKRGIDSSLAKFFGIGYSSEGWNNITELAKSINIPE
HHHHHHHEECCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCH
EILVDTGLAIKNDKGNLYDRFRGRVMFPIRNIQGNVIAYGGRVTEDSDGVKYINSPETLV
HHHHHCCCEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCEECCCCCCCEEECCCCEEE
FQKNNILYGLYEYRERKKQYPDLINQSLVVVEGYMDVVGLAQHGFYAAVATLGTAFSPNH
EECCCEEEHHHHHHHHHHHCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AKILFRETSSVILCFDGDEAGQKAALRTIKILLPMLDGNKKLKILTLPDKDDPDDYIKKY
CEEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
GLERFLTALDNSLAVADFVIDNLIQGKDLRKAEAKAEVLENLKNFLADVEDNIYSESITA
HHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIADKIGIKVEQFKNLLKIRKQTTLNVNRQQNIQQKKLAKNLLLEEFVLAELFVNIADFR
HHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILQHTNDFEIFATSKNLDILAKSLKILKEDSSNQIEAVILIQLLAEDYPDYREYFFELLS
HEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
YGIRNTQKKYAEDKYQEQMFVMLKRVENSSVKKRLKYLGSLPFRSDVQEMERKYLVAKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
NSNII
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]