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The map label for this gene is dacD [H]

Identifier: 134301929

GI number: 134301929

Start: 900328

End: 901662

Strand: Direct

Name: dacD [H]

Synonym: FTW_0936

Alternate gene names: 134301929

Gene position: 900328-901662 (Clockwise)

Preceding gene: 134301915

Following gene: 134301930

Centisome position: 47.42

GC content: 35.96

Gene sequence:

>1335_bases
ATGAAATTGATAAAAATAGCTTTATTAACAGCTAGTATAACAGCGGGGGTAGCTTTTGCTGCGCCTAATATATCTGCCTA
CTCTGATCCTTATTTCAATGGAGCTAATGGACTCGCGCAGAAAGATATTATTATAAGACCTGCAAACATTGAGCTAGATG
CTCCAGCTTGGGTAACGATGGATTATCGTACTGGAGATATTGTTAGTGAAAAAAATATGGACGTCAGAAGAGCCCCTGCA
AGCTTAACTAAAATCATGACTTCCTATATAGTTGCTAGTGAAATAAAAGCTGGTAATCTAAGCTGGGATACAATGATCCC
AATTAGTGAAAATGCTGCTTCTACAGGTGGTTCAAAGATGTATGTTAAAGCCGGTGCTAAGGTATCTGTAAGAAATCTTG
TAACTGGTATGGATGTAGTGTCTGGTAATGATGCTACTATTGCTCTTGCTGAATATATCGGCGGAACTACACAAGCCTTT
ACTGATTTAATGAATCAAACAGCAAAGGCTATTGGCATGAATAATACTCATTTTGCTAATCCAGATGGACTTCCAGGTGG
TGAGCAATATACTACTGCTCATGATATGGCGTTGCTTGCAAGATCATACATATATAATTTCCCAGAAGCATATAAAGTAT
ACGACGATAAGGGCTTAGTTTGGAATGCCACAAAACAAGACTCGGTAAGTATTGCTGATCGTAAACAGTGCTTACCTAAG
TTTGATCGCGCTACTGGAAATGTCATAGAGAGCTATACGGTCAAAGATCTTGATGATCAAGCTAAAGATAAATGTAATAA
GCTTTTTCCTAAAGGTGATAATTTCGTATTACAAAATAATAGAAATAGATTACTATTTACATTTGATGGGGCTGATGGTA
TGAAGACTGGTCATACTGATGCTGCTGGATACTGTCTAGTTTCATCTGCTAAACAAGATGGCGAGAGATTTATTTCTGTA
GTTCTTGGTACTACTAGTTCTGCTAAGAGAGATTCAGAATCAGCTAAATTACTAAGATATGCTCTGAGTAAGTATGAAAA
TGTACTTTTATACAAAGCAAATTCTCCAGTTACAATTAGTGCTGATAATATCCCTAATGCAAAAGCTGGACAAAAGTTAA
CTGTAGCTTCAAATCAAAATATTTATAAAACAGTACCTAAAACTTATGTCCCTTATCTAAAGCAAGGTATAGAATTTAAT
CCAAATTTGAACGCTCCTATTAAAACAGGTCAAACTGTAGGTAATTTAGTGATTACACTAGGTGATACTAAGGAGGAAAT
TGCTAGTATACCTGTAGTTGCTATGAATAATGTTTCACAAAAAGGTTGGTGGTAA

Upstream 100 bases:

>100_bases
TATCACAAAAACCTTTGTATTTATAACATATAAATATTAAAATACTTAAAGTTATGAGCTTAAATATATACACTTTGATT
AAAATTTAAAGAGGGAAAAT

Downstream 100 bases:

>100_bases
TAACCACAGAATTTGTAATTAAGGATTTATAAATGTCTGAAAGTAATAATCACAATCAACAAGAAACTTTTTTTGAGTTT
CCTTGCCAATTTCCAATAAA

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKLIKIALLTASITAGVAFAAPNISAYSDPYFNGANGLAQKDIIIRPANIELDAPAWVTMDYRTGDIVSEKNMDVRRAPA
SLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKMYVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAF
TDLMNQTAKAIGMNNTHFANPDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTDAAGYCLVSSAKQDGERFISV
VLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTISADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFN
PNLNAPIKTGQTVGNLVITLGDTKEEIASIPVVAMNNVSQKGWW

Sequences:

>Translated_444_residues
MKLIKIALLTASITAGVAFAAPNISAYSDPYFNGANGLAQKDIIIRPANIELDAPAWVTMDYRTGDIVSEKNMDVRRAPA
SLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKMYVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAF
TDLMNQTAKAIGMNNTHFANPDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTDAAGYCLVSSAKQDGERFISV
VLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTISADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFN
PNLNAPIKTGQTVGNLVITLGDTKEEIASIPVVAMNNVSQKGWW
>Mature_444_residues
MKLIKIALLTASITAGVAFAAPNISAYSDPYFNGANGLAQKDIIIRPANIELDAPAWVTMDYRTGDIVSEKNMDVRRAPA
SLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKMYVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAF
TDLMNQTAKAIGMNNTHFANPDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTDAAGYCLVSSAKQDGERFISV
VLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTISADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFN
PNLNAPIKTGQTVGNLVITLGDTKEEIASIPVVAMNNVSQKGWW

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=419, Percent_Identity=31.2649164677804, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1787062, Length=406, Percent_Identity=33.9901477832512, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786851, Length=454, Percent_Identity=30.3964757709251, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48100; Mature: 48100

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIKIALLTASITAGVAFAAPNISAYSDPYFNGANGLAQKDIIIRPANIELDAPAWVTM
CCEEEEEEEHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCEEEEE
DYRTGDIVSEKNMDVRRAPASLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKM
ECCCCCEECCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEE
YVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAFTDLMNQTAKAIGMNNTHFAN
EEECCCEEEHHHHHHCCEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
PDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHCCH
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTD
HHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCC
AAGYCLVSSAKQDGERFISVVLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTIS
CCCEEEEECCHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCEEEE
ADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFNPNLNAPIKTGQTVGNLVITL
CCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
GDTKEEIASIPVVAMNNVSQKGWW
CCCHHHHHCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKLIKIALLTASITAGVAFAAPNISAYSDPYFNGANGLAQKDIIIRPANIELDAPAWVTM
CCEEEEEEEHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCEEEEE
DYRTGDIVSEKNMDVRRAPASLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKM
ECCCCCEECCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEE
YVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAFTDLMNQTAKAIGMNNTHFAN
EEECCCEEEHHHHHHCCEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
PDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHCCH
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTD
HHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCC
AAGYCLVSSAKQDGERFISVVLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTIS
CCCEEEEECCHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCEEEE
ADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFNPNLNAPIKTGQTVGNLVITL
CCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEE
GDTKEEIASIPVVAMNNVSQKGWW
CCCHHHHHCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]