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The map label for this gene is lip2 [H]

Identifier: 134301852

GI number: 134301852

Start: 813271

End: 814191

Strand: Reverse

Name: lip2 [H]

Synonym: FTW_0835

Alternate gene names: 134301852

Gene position: 814191-813271 (Counterclockwise)

Preceding gene: 134301853

Following gene: 134301851

Centisome position: 42.89

GC content: 32.57

Gene sequence:

>921_bases
ATGCCATACAATCCATCATTAGAAGCATTATTAGACACTCCTGAAATAAGAAGAATTAAAAAGCTTGATTTAACACAACA
GCGTGAAATTTTTACTAGTCTTTCGGTAGAACAGCTTAAGCGTTTACCGCGTCCTGATATTCTTGAAGAAGATATTAAGC
TAGAAAATAATACCATACTAAGGCATTATCAACCTAAAAAATCCTCTAATAAAGCTGTACTTTTTATCCATGGTGGTGGA
TGGTGCTTAGGTTCGATAAATACTTATGATCATGTATGTAGGTACCTATGTGATCAAGGAAATTTAAATATCTTCTCGCT
AGAATATGGTCTAGCACCTGAACAAAAATATCCAGCAGCTGTTGATCATGCTTTGTACGCTTACGATTGGCTATATCAAA
ATATCAATAAATTTAAAATTTCTGCTGAAAATATCTTTGTAATGGGTGATAGCGCTGGAGGTAATCTAGTGACTATAGTT
TGTCATGAACGTCAAGAAAATATGCCAAAAGCACAAATTTTGGTCTACCCTGCTGTTGATATGTATACGAAATACGATAG
TAATAAAAAATTTGATGAAAACAAATATCATTTAACTACAGAATGGTGTGAACTATTCCTCAAAGCGTATATAGGTGAAG
ATGTTATGTCAGAGCCTAAAAAACTTAGACATCCTACAATATCTCCACTTTTTTATAAAGATACAAAGCAGCCAGATACT
CTAATTGTAGCAGCTACTCATGATATCCTAATAGATGGTATTTATGCTTATGAAGAAAAATTAAAACAGCAAGGAACTTA
TGTTGAAACTTACTACGATGATGAAATGTTTCATGGTTTCATGGGCATACTTGGTATAACTCCGTTTGAAAATCCTAAAA
TAGCTTTGAATAAGGTGATTGAGTTTATAAACAAAAGATAA

Upstream 100 bases:

>100_bases
TTTAGCAACTTTAGATCGAGCTACAAGAGAGCTTTCAAGAAAATTAGAGAAACTAGATATTGATATTATAAGAGTACACA
AGATTTAATAAGGAAATATT

Downstream 100 bases:

>100_bases
ATATTGGTTTGATATATGACTCCATTTTTTAAAACTGATAAAAAATCTGGCGAAAAATCAGAAGAACTTATTAAAAGATT
TAATGACTTACCTGCTTTTG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPYNPSLEALLDTPEIRRIKKLDLTQQREIFTSLSVEQLKRLPRPDILEEDIKLENNTILRHYQPKKSSNKAVLFIHGGG
WCLGSINTYDHVCRYLCDQGNLNIFSLEYGLAPEQKYPAAVDHALYAYDWLYQNINKFKISAENIFVMGDSAGGNLVTIV
CHERQENMPKAQILVYPAVDMYTKYDSNKKFDENKYHLTTEWCELFLKAYIGEDVMSEPKKLRHPTISPLFYKDTKQPDT
LIVAATHDILIDGIYAYEEKLKQQGTYVETYYDDEMFHGFMGILGITPFENPKIALNKVIEFINKR

Sequences:

>Translated_306_residues
MPYNPSLEALLDTPEIRRIKKLDLTQQREIFTSLSVEQLKRLPRPDILEEDIKLENNTILRHYQPKKSSNKAVLFIHGGG
WCLGSINTYDHVCRYLCDQGNLNIFSLEYGLAPEQKYPAAVDHALYAYDWLYQNINKFKISAENIFVMGDSAGGNLVTIV
CHERQENMPKAQILVYPAVDMYTKYDSNKKFDENKYHLTTEWCELFLKAYIGEDVMSEPKKLRHPTISPLFYKDTKQPDT
LIVAATHDILIDGIYAYEEKLKQQGTYVETYYDDEMFHGFMGILGITPFENPKIALNKVIEFINKR
>Mature_305_residues
PYNPSLEALLDTPEIRRIKKLDLTQQREIFTSLSVEQLKRLPRPDILEEDIKLENNTILRHYQPKKSSNKAVLFIHGGGW
CLGSINTYDHVCRYLCDQGNLNIFSLEYGLAPEQKYPAAVDHALYAYDWLYQNINKFKISAENIFVMGDSAGGNLVTIVC
HERQENMPKAQILVYPAVDMYTKYDSNKKFDENKYHLTTEWCELFLKAYIGEDVMSEPKKLRHPTISPLFYKDTKQPDTL
IVAATHDILIDGIYAYEEKLKQQGTYVETYYDDEMFHGFMGILGITPFENPKIALNKVIEFINKR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=295, Percent_Identity=25.4237288135593, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI157041239, Length=323, Percent_Identity=22.2910216718266, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI206597554, Length=354, Percent_Identity=22.8813559322034, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI68051721, Length=282, Percent_Identity=23.758865248227, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=203, Percent_Identity=26.1083743842365, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567059, Length=297, Percent_Identity=26.2626262626263, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17540028, Length=306, Percent_Identity=27.4509803921569, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI72001146, Length=134, Percent_Identity=37.3134328358209, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71996133, Length=141, Percent_Identity=36.1702127659575, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI115533410, Length=88, Percent_Identity=37.5, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=88, Percent_Identity=37.5, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24656084, Length=117, Percent_Identity=32.4786324786325, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656076, Length=117, Percent_Identity=32.4786324786325, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130169, Length=117, Percent_Identity=32.4786324786325, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 35510; Mature: 35379

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYNPSLEALLDTPEIRRIKKLDLTQQREIFTSLSVEQLKRLPRPDILEEDIKLENNTIL
CCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHCEECCCEEE
RHYQPKKSSNKAVLFIHGGGWCLGSINTYDHVCRYLCDQGNLNIFSLEYGLAPEQKYPAA
EEECCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
VDHALYAYDWLYQNINKFKISAENIFVMGDSAGGNLVTIVCHERQENMPKAQILVYPAVD
HHHHHHHHHHHHHCCHHEEEEECEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEEECHH
MYTKYDSNKKFDENKYHLTTEWCELFLKAYIGEDVMSEPKKLRHPTISPLFYKDTKQPDT
HHEECCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCHHHHCCCCCCCEEECCCCCCCE
LIVAATHDILIDGIYAYEEKLKQQGTYVETYYDDEMFHGFMGILGITPFENPKIALNKVI
EEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHH
EFINKR
HHHHCC
>Mature Secondary Structure 
PYNPSLEALLDTPEIRRIKKLDLTQQREIFTSLSVEQLKRLPRPDILEEDIKLENNTIL
CCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHCEECCCEEE
RHYQPKKSSNKAVLFIHGGGWCLGSINTYDHVCRYLCDQGNLNIFSLEYGLAPEQKYPAA
EEECCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHH
VDHALYAYDWLYQNINKFKISAENIFVMGDSAGGNLVTIVCHERQENMPKAQILVYPAVD
HHHHHHHHHHHHHCCHHEEEEECEEEEEECCCCCCEEEEEEECCCCCCCCEEEEEEECHH
MYTKYDSNKKFDENKYHLTTEWCELFLKAYIGEDVMSEPKKLRHPTISPLFYKDTKQPDT
HHEECCCCCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCHHHHCCCCCCCEEECCCCCCCE
LIVAATHDILIDGIYAYEEKLKQQGTYVETYYDDEMFHGFMGILGITPFENPKIALNKVI
EEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHH
EFINKR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]