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The map label for this gene is ogt [H]

Identifier: 134301800

GI number: 134301800

Start: 750971

End: 751543

Strand: Reverse

Name: ogt [H]

Synonym: FTW_0773

Alternate gene names: 134301800

Gene position: 751543-750971 (Counterclockwise)

Preceding gene: 134301803

Following gene: 134301799

Centisome position: 39.59

GC content: 31.41

Gene sequence:

>573_bases
ATGCTCAAAGAAACTCATCAAATTTATTTAGCTGCGATCAATAAAAAGAGCTATAAAACCGTATTTTATGCTCAAGAGAT
TCAGACGCCTATTGGTAATCTTATTGCAGTTGCTGATGATGATTATCTATATGCTTGCTTTTTTATTAGTGATTCGAATA
TCTATTCTATAGAAAAATTATTAAAAATTTACAATGCAAAAATATCTTTCAATGCAGACAATATAACCAATCAGACTAAA
GATCAACTAAATAAATATTTCAAAAGAGAACTTAAAAGCTTCTCAGTCCCACTACGACTAACAGGCACAGACTTTCAAAA
ACAAGTTTGGCGCGAGCTAATAAAGATCCCTTATGGAGAAACTATCAGCTATCGTAAAGAAGCTACAAATATTGGTAAAC
CAACTGCTTTTAGAGCTGTAGCTAATGCTAATGGTAAGAATCTATTACCCATAATTATTCCTTGTCATCGCGTAATTAAT
ACTAACGGTAAACTTGGTGGCTATACCGGTGGATTATATAAAAAAGAGTTTTTGTTAAATCTAGAACACTCGATTCTAAT
ACACAAAAATTAG

Upstream 100 bases:

>100_bases
ATACCAATAATTAAAAATACTACAGGTAAAAACCTATAAAATTTACTATTAAACATAGTTTGAGTTATCATTTAAAAGAC
ATAATAAACATAATATACCT

Downstream 100 bases:

>100_bases
ATTATAAGTTGCTCAATTATCAATTATCAATTATCATAAAAATGATAAGCACTTATGCTTTATTATGTCAAACTTTTAAT
AGGAGAAAATAATGAAAAAA

Product: methylated-DNA--protein-cysteine methyltransferase family protein

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKLLKIYNAKISFNADNITNQTK
DQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGETISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVIN
TNGKLGGYTGGLYKKEFLLNLEHSILIHKN

Sequences:

>Translated_190_residues
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKLLKIYNAKISFNADNITNQTK
DQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGETISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVIN
TNGKLGGYTGGLYKKEFLLNLEHSILIHKN
>Mature_190_residues
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKLLKIYNAKISFNADNITNQTK
DQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGETISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVIN
TNGKLGGYTGGLYKKEFLLNLEHSILIHKN

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=163, Percent_Identity=31.2883435582822, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787596, Length=167, Percent_Identity=37.125748502994, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI1788542, Length=146, Percent_Identity=38.3561643835616, Blast_Score=107, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=50, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=50, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=96, Percent_Identity=45.8333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137554, Length=92, Percent_Identity=47.8260869565217, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21759; Mature: 21759

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKL
CCCCCEEEEEEEECCCCCEEEEEHHHHCCCCCCEEEEECCCEEEEEEEEECCCCHHHHHH
LKIYNAKISFNADNITNQTKDQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGE
HHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCC
TISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVINTNGKLGGYTGGLYKKEFLLN
CCHHHHHHCCCCCCHHHHEEECCCCCEEEEEEEECHHHHCCCCCCCCCCCCCHHHHHHEE
LEHSILIHKN
CCCEEEEECC
>Mature Secondary Structure
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKL
CCCCCEEEEEEEECCCCCEEEEEHHHHCCCCCCEEEEECCCEEEEEEEEECCCCHHHHHH
LKIYNAKISFNADNITNQTKDQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGE
HHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCC
TISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVINTNGKLGGYTGGLYKKEFLLN
CCHHHHHHCCCCCCHHHHEEECCCCCEEEEEEEECHHHHCCCCCCCCCCCCCHHHHHHEE
LEHSILIHKN
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA