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The map label for this gene is xasA [C]

Identifier: 134301798

GI number: 134301798

Start: 749108

End: 750487

Strand: Direct

Name: xasA [C]

Synonym: FTW_0771

Alternate gene names: 134301798

Gene position: 749108-750487 (Clockwise)

Preceding gene: 134301797

Following gene: 134301801

Centisome position: 39.46

GC content: 35.65

Gene sequence:

>1380_bases
ATGCATATATCTAACAAAAAAATTTCGACATTATCGCTAACAATGCTAAATATTTCAGCGATAATTAGTCTAAGCTCGAT
TGCTTATATGGCGACAATTGGCTTACAGAGTGTTTTCTTTTATCTAATTGCCGCAGTTACATTTCTATTACCAACATCAC
TTATCTGCGCTGAGCTTAGTAGTATGATAACGCAAAATAATGGTGGTGTTTTTAGCTGGGTCAAAGCTGGCTTAGGTGAA
AAAGCGGGTGTGTTAGCGATGTGGTTGGAGTGGTTTAATAACGTTGTAGGTTTTCCTAGCTCAGTAACAGCGCTAATCGC
AACATTTTCATATATTGGCTTTAGAAATTTTGCTGAAAATACTCACACAAGTACAACGTTTTGGTTAATAATGGTGACTG
TTTTTGTCGGCATAAGCTTATTTAACTGCCTACCATTAAGGAAAGTAGTAATCCTTAATATTATTGGTGCAGTATTCGGA
ATGATAATTCCTGGAGTACTGTTAGTTTCTGGGGCAATATACTTTGTGGTTACTGGACAGAGTAATTTAGAGTATCATGG
TTTGAGTGATTTGTTACCAGTATTTTCTATAGGGACTTATGCACTACTTGTCAAAACACTCTCTTCATATTCCGGTATTC
AGTCAGTAGCTTTCCACATGACAAATATTGAGAATCCAGAGAAAAATATTCCTAAATCTATATTGATAGCTACTTTGGTA
ATTGTATCGTTAACTATCTTAACCACGATTGGTTTGACAGTAATTATTCCTGTGAAAGATGTTAATGTTTTAAATGGTTT
GATACAAGGTATTTCGCAAGTGCTGAATATAATCGGTTTAGGTGATGTTAAGCCAGTTGTTGTGATAATGATAAGCTTAG
GTATGTTAGCTGCACTTAGTACATGGATCTTGGGGCCTGCACGTGGGATGCAGACAGCAGCAGAACAGGAGTTTTTCCCT
AAAGTCATGGCTGGCAAAAATAGATTTGGCATGCCAGTAAATATGCTACTAATTCAGATTTTGATAGTTTTAGTGTTATC
ATCCGTATTCTTAGTAATGCCATCAGTATATGCGGCATTTGCGTTATTGATTGCTATTACGTCACAGTTTACCGTAGTTA
TGTGGATAATGGTTTTCATCGCAGCTATTAGACTTAGATTTATACGACCAGATACTCACAGAGTTTTTCACGTAGGTAGG
AGAAACTCTAATTGGCTACTTATAACTATGTCACTAGTAGCTATATTTATGTGTATGCTAGGTTTTATACTTGGATTATT
TCCACCGAGCTTCTCACATGTTAAAAATGTCTTCCAGTATACGATGATATTGGTTGTAGCAGATATTATAATTATCGCAA
TTCCATTAATATGGATTTAG

Upstream 100 bases:

>100_bases
TATTAGCTATATATTCAGCAGGTGCGTACGGAAAGGTTTTGGCTAGTGAATATAATCTAAGACCAAGTGTTCAAGAGTAT
TTTATATAGGGTTACAGACA

Downstream 100 bases:

>100_bases
TTACATAGAAAAAGAATTCTATAGTTTATATATGATAGATACTATTTAATTTCCACCTGTTTACCATCTTTATCAAAGGC
ACAGCGAACTGTAGTCCCTC

Product: amino acid transporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MHISNKKISTLSLTMLNISAIISLSSIAYMATIGLQSVFFYLIAAVTFLLPTSLICAELSSMITQNNGGVFSWVKAGLGE
KAGVLAMWLEWFNNVVGFPSSVTALIATFSYIGFRNFAENTHTSTTFWLIMVTVFVGISLFNCLPLRKVVILNIIGAVFG
MIIPGVLLVSGAIYFVVTGQSNLEYHGLSDLLPVFSIGTYALLVKTLSSYSGIQSVAFHMTNIENPEKNIPKSILIATLV
IVSLTILTTIGLTVIIPVKDVNVLNGLIQGISQVLNIIGLGDVKPVVVIMISLGMLAALSTWILGPARGMQTAAEQEFFP
KVMAGKNRFGMPVNMLLIQILIVLVLSSVFLVMPSVYAAFALLIAITSQFTVVMWIMVFIAAIRLRFIRPDTHRVFHVGR
RNSNWLLITMSLVAIFMCMLGFILGLFPPSFSHVKNVFQYTMILVVADIIIIAIPLIWI

Sequences:

>Translated_459_residues
MHISNKKISTLSLTMLNISAIISLSSIAYMATIGLQSVFFYLIAAVTFLLPTSLICAELSSMITQNNGGVFSWVKAGLGE
KAGVLAMWLEWFNNVVGFPSSVTALIATFSYIGFRNFAENTHTSTTFWLIMVTVFVGISLFNCLPLRKVVILNIIGAVFG
MIIPGVLLVSGAIYFVVTGQSNLEYHGLSDLLPVFSIGTYALLVKTLSSYSGIQSVAFHMTNIENPEKNIPKSILIATLV
IVSLTILTTIGLTVIIPVKDVNVLNGLIQGISQVLNIIGLGDVKPVVVIMISLGMLAALSTWILGPARGMQTAAEQEFFP
KVMAGKNRFGMPVNMLLIQILIVLVLSSVFLVMPSVYAAFALLIAITSQFTVVMWIMVFIAAIRLRFIRPDTHRVFHVGR
RNSNWLLITMSLVAIFMCMLGFILGLFPPSFSHVKNVFQYTMILVVADIIIIAIPLIWI
>Mature_459_residues
MHISNKKISTLSLTMLNISAIISLSSIAYMATIGLQSVFFYLIAAVTFLLPTSLICAELSSMITQNNGGVFSWVKAGLGE
KAGVLAMWLEWFNNVVGFPSSVTALIATFSYIGFRNFAENTHTSTTFWLIMVTVFVGISLFNCLPLRKVVILNIIGAVFG
MIIPGVLLVSGAIYFVVTGQSNLEYHGLSDLLPVFSIGTYALLVKTLSSYSGIQSVAFHMTNIENPEKNIPKSILIATLV
IVSLTILTTIGLTVIIPVKDVNVLNGLIQGISQVLNIIGLGDVKPVVVIMISLGMLAALSTWILGPARGMQTAAEQEFFP
KVMAGKNRFGMPVNMLLIQILIVLVLSSVFLVMPSVYAAFALLIAITSQFTVVMWIMVFIAAIRLRFIRPDTHRVFHVGR
RNSNWLLITMSLVAIFMCMLGFILGLFPPSFSHVKNVFQYTMILVVADIIIIAIPLIWI

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=444, Percent_Identity=23.8738738738739, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI48994921, Length=437, Percent_Identity=22.1967963386728, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081798, Length=425, Percent_Identity=23.0588235294118, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50164; Mature: 50164

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISNKKISTLSLTMLNISAIISLSSIAYMATIGLQSVFFYLIAAVTFLLPTSLICAELS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMITQNNGGVFSWVKAGLGEKAGVLAMWLEWFNNVVGFPSSVTALIATFSYIGFRNFAEN
HHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
THTSTTFWLIMVTVFVGISLFNCLPLRKVVILNIIGAVFGMIIPGVLLVSGAIYFVVTGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
SNLEYHGLSDLLPVFSIGTYALLVKTLSSYSGIQSVAFHMTNIENPEKNIPKSILIATLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCHHHHHHHHH
IVSLTILTTIGLTVIIPVKDVNVLNGLIQGISQVLNIIGLGDVKPVVVIMISLGMLAALS
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TWILGPARGMQTAAEQEFFPKVMAGKNRFGMPVNMLLIQILIVLVLSSVFLVMPSVYAAF
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLIAITSQFTVVMWIMVFIAAIRLRFIRPDTHRVFHVGRRNSNWLLITMSLVAIFMCML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCEEEEHHHHHHHHHHHH
GFILGLFPPSFSHVKNVFQYTMILVVADIIIIAIPLIWI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHISNKKISTLSLTMLNISAIISLSSIAYMATIGLQSVFFYLIAAVTFLLPTSLICAELS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMITQNNGGVFSWVKAGLGEKAGVLAMWLEWFNNVVGFPSSVTALIATFSYIGFRNFAEN
HHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
THTSTTFWLIMVTVFVGISLFNCLPLRKVVILNIIGAVFGMIIPGVLLVSGAIYFVVTGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
SNLEYHGLSDLLPVFSIGTYALLVKTLSSYSGIQSVAFHMTNIENPEKNIPKSILIATLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCHHHHHHHHH
IVSLTILTTIGLTVIIPVKDVNVLNGLIQGISQVLNIIGLGDVKPVVVIMISLGMLAALS
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TWILGPARGMQTAAEQEFFPKVMAGKNRFGMPVNMLLIQILIVLVLSSVFLVMPSVYAAF
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLIAITSQFTVVMWIMVFIAAIRLRFIRPDTHRVFHVGRRNSNWLLITMSLVAIFMCML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCCCEEEEHHHHHHHHHHHH
GFILGLFPPSFSHVKNVFQYTMILVVADIIIIAIPLIWI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]