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The map label for this gene is ubiH [C]

Identifier: 134301763

GI number: 134301763

Start: 707207

End: 708418

Strand: Direct

Name: ubiH [C]

Synonym: FTW_0728

Alternate gene names: 134301763

Gene position: 707207-708418 (Clockwise)

Preceding gene: 134301762

Following gene: 134301764

Centisome position: 37.25

GC content: 34.74

Gene sequence:

>1212_bases
ATGAATGCTAAATATGATGTGGCAATTGTTGGAGGTGGGATAGTTGGTTTATTTACTTCTTTAGCTCTAGCCAAGACTGG
ATGTAAAATTATCCATATTGAAAAAGATCAGCTGCAAGTAAAAAATGATAATAGAAGTATTGCGGTATCTTATTCATCAA
TAGCTTTTTTAAATACACTTGGTTTGTGGGATAAGGTTGCAAGTAAAACTCAAGCTATTAAAAAAGTTCATGTTTCAGAT
AAGGGCAGATATGGACGAGCTGAGATTTTTGCTAAAGATGAAAATTTGCCATTTTTAGGAGCTATTGCGCCGATGCAGGA
GCTTCTGACAGTTGCTTTACAAAGTGTTGCTGCTAATCCAAATATTATCAAATCTTTTGAAACAAATGTTATTGACCTTG
CTAAACATGATGATAAGTATTCTTTAGTAGTCAAGCAACAAGAACAGACTAAAACTATACGAGCACAGCTGATAATAGCT
TGTGATGGCGCTAATTCAAGCTTAAGAAAAATGCTAAATATCACAGCTAAGACAACTGATTATCAGCAAGATGCGCTTGT
TTTTGATATTCAAACAGAGCTTGATAACAATAATACAGCTTTTGAAAGGTTTATGACTGATGGTGTACTAGCGATGTTAC
CTAAAGTAAAAACAACTATGGGCTGTGTTTGGACTATTGATAGAGATAATTCAAAAGCAAAGCTAACCTTAGACAATAAA
GAGTTTGAACAGTTAGTCCAAGATCGCTTCGGCTATAGGTTAGGACAGATTAAACTTACCTCAAAGCCTGCGGTTTTTCC
TTTATACCTTGTCCAAGCAGAGCAAGTCTACAAAAACAATGTACTTTTTTTTGGTAATGCATTGCATTTTTTACATCCAG
TATCCGGTCAGGGAATGAACCTAAGTATTCGTGATATTGGTTTTTTGTATGATTTGCTTGTAGAGAGTGATTTTTCTCAG
AACTCAATTACAGCTGTTTTAGCAGAGTTTGCCAAAGTTAGAAAACCAGATCATGATAGAACAATTTTTGTCACACATGG
ATTTGTAAAATGGTTTGTATCTAATGATACTAAATTTGTTGCAAGTAGAAATGCTGGTTTACATCTGTTACAAAGAAGTA
AGTTAGCTAAAAAAGTTTTATCAAGAGTTATGATGGGCAAGCTTAGCAAAGGTTCAACTCTGATGAGAAAGGTGGTCGAG
GATGAGCGTTAG

Upstream 100 bases:

>100_bases
TAGGTGAAGATGAAAGTTGTGAAGAAGATTTTATCGAGTTGCTTGAGTATACAAAAGTAGCAGTTTTACTTATTGATAGT
GAAAAAATTTAGGTATTAAC

Downstream 100 bases:

>100_bases
AAATATTCAAAAAGATGCTGTGATTGTCGGTGGTGGTATGGTAGGCTTAAGCCTTGCTCTAGCATTGCACCAAAATGGTT
TGCAGGTTGCGCTTGTTGAG

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MNAKYDVAIVGGGIVGLFTSLALAKTGCKIIHIEKDQLQVKNDNRSIAVSYSSIAFLNTLGLWDKVASKTQAIKKVHVSD
KGRYGRAEIFAKDENLPFLGAIAPMQELLTVALQSVAANPNIIKSFETNVIDLAKHDDKYSLVVKQQEQTKTIRAQLIIA
CDGANSSLRKMLNITAKTTDYQQDALVFDIQTELDNNNTAFERFMTDGVLAMLPKVKTTMGCVWTIDRDNSKAKLTLDNK
EFEQLVQDRFGYRLGQIKLTSKPAVFPLYLVQAEQVYKNNVLFFGNALHFLHPVSGQGMNLSIRDIGFLYDLLVESDFSQ
NSITAVLAEFAKVRKPDHDRTIFVTHGFVKWFVSNDTKFVASRNAGLHLLQRSKLAKKVLSRVMMGKLSKGSTLMRKVVE
DER

Sequences:

>Translated_403_residues
MNAKYDVAIVGGGIVGLFTSLALAKTGCKIIHIEKDQLQVKNDNRSIAVSYSSIAFLNTLGLWDKVASKTQAIKKVHVSD
KGRYGRAEIFAKDENLPFLGAIAPMQELLTVALQSVAANPNIIKSFETNVIDLAKHDDKYSLVVKQQEQTKTIRAQLIIA
CDGANSSLRKMLNITAKTTDYQQDALVFDIQTELDNNNTAFERFMTDGVLAMLPKVKTTMGCVWTIDRDNSKAKLTLDNK
EFEQLVQDRFGYRLGQIKLTSKPAVFPLYLVQAEQVYKNNVLFFGNALHFLHPVSGQGMNLSIRDIGFLYDLLVESDFSQ
NSITAVLAEFAKVRKPDHDRTIFVTHGFVKWFVSNDTKFVASRNAGLHLLQRSKLAKKVLSRVMMGKLSKGSTLMRKVVE
DER
>Mature_403_residues
MNAKYDVAIVGGGIVGLFTSLALAKTGCKIIHIEKDQLQVKNDNRSIAVSYSSIAFLNTLGLWDKVASKTQAIKKVHVSD
KGRYGRAEIFAKDENLPFLGAIAPMQELLTVALQSVAANPNIIKSFETNVIDLAKHDDKYSLVVKQQEQTKTIRAQLIIA
CDGANSSLRKMLNITAKTTDYQQDALVFDIQTELDNNNTAFERFMTDGVLAMLPKVKTTMGCVWTIDRDNSKAKLTLDNK
EFEQLVQDRFGYRLGQIKLTSKPAVFPLYLVQAEQVYKNNVLFFGNALHFLHPVSGQGMNLSIRDIGFLYDLLVESDFSQ
NSITAVLAEFAKVRKPDHDRTIFVTHGFVKWFVSNDTKFVASRNAGLHLLQRSKLAKKVLSRVMMGKLSKGSTLMRKVVE
DER

Specific function: Oxygenase That Introduces The Hydroxyl Group At Carbon Four Of 2-Octaprenyl-6-Methoxyphenol Resulting In The Formation Of 2-Octaprenyl-6-Methoxy-1,4-Benzoquinone. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=332, Percent_Identity=24.3975903614458, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI32967307, Length=210, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789274, Length=394, Percent_Identity=27.4111675126904, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786883, Length=403, Percent_Identity=29.2803970223325, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789273, Length=307, Percent_Identity=28.6644951140065, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25146631, Length=362, Percent_Identity=24.8618784530387, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19920760, Length=389, Percent_Identity=22.3650385604113, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 45060; Mature: 45060

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKYDVAIVGGGIVGLFTSLALAKTGCKIIHIEKDQLQVKNDNRSIAVSYSSIAFLNTL
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEHHHEEEECCCCEEEEEEHHHHHHHHH
GLWDKVASKTQAIKKVHVSDKGRYGRAEIFAKDENLPFLGAIAPMQELLTVALQSVAANP
HHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCC
NIIKSFETNVIDLAKHDDKYSLVVKQQEQTKTIRAQLIIACDGANSSLRKMLNITAKTTD
CHHHHCCCCEEEEECCCCCEEEEEECCHHHHEEEEEEEEEECCCCHHHHHHHHHHCCCCC
YQQDALVFDIQTELDNNNTAFERFMTDGVLAMLPKVKTTMGCVWTIDRDNSKAKLTLDNK
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHCHHHHCEEEEEEEECCCCEEEEEECCH
EFEQLVQDRFGYRLGQIKLTSKPAVFPLYLVQAEQVYKNNVLFFGNALHFLHPVSGQGMN
HHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEHHHHHHCCEEEEECHHHEECCCCCCCCE
LSIRDIGFLYDLLVESDFSQNSITAVLAEFAKVRKPDHDRTIFVTHGFVKWFVSNDTKFV
EEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCEEEE
ASRNAGLHLLQRSKLAKKVLSRVMMGKLSKGSTLMRKVVEDER
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNAKYDVAIVGGGIVGLFTSLALAKTGCKIIHIEKDQLQVKNDNRSIAVSYSSIAFLNTL
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEHHHEEEECCCCEEEEEEHHHHHHHHH
GLWDKVASKTQAIKKVHVSDKGRYGRAEIFAKDENLPFLGAIAPMQELLTVALQSVAANP
HHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHCCCC
NIIKSFETNVIDLAKHDDKYSLVVKQQEQTKTIRAQLIIACDGANSSLRKMLNITAKTTD
CHHHHCCCCEEEEECCCCCEEEEEECCHHHHEEEEEEEEEECCCCHHHHHHHHHHCCCCC
YQQDALVFDIQTELDNNNTAFERFMTDGVLAMLPKVKTTMGCVWTIDRDNSKAKLTLDNK
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHCHHHHCEEEEEEEECCCCEEEEEECCH
EFEQLVQDRFGYRLGQIKLTSKPAVFPLYLVQAEQVYKNNVLFFGNALHFLHPVSGQGMN
HHHHHHHHHHCCEEEEEEECCCCCEEEEEEEEHHHHHHCCEEEEECHHHEECCCCCCCCE
LSIRDIGFLYDLLVESDFSQNSITAVLAEFAKVRKPDHDRTIFVTHGFVKWFVSNDTKFV
EEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCEEEE
ASRNAGLHLLQRSKLAKKVLSRVMMGKLSKGSTLMRKVVEDER
EECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]