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The map label for this gene is rne [H]

Identifier: 134301754

GI number: 134301754

Start: 698907

End: 701564

Strand: Reverse

Name: rne [H]

Synonym: FTW_0719

Alternate gene names: 134301754

Gene position: 701564-698907 (Counterclockwise)

Preceding gene: 134301758

Following gene: 134301753

Centisome position: 36.95

GC content: 33.07

Gene sequence:

>2658_bases
ATGAAAAGAATACTAATAAATAGCAAGAGTGGCGAAGAAACAAGAATTGCCACCCTAGATAATGGCAAATTAATAGATTT
AGACATTGAGAGCGTTGATAGAGAACAAAAAAAAGCAAATATTTACAAAGGTTATATTTCAAGAATTGAACCAAGTTTAA
ATGCTATATTTGTTAACTATGGTGAAGAAAAAAATGGTTTTTTACCATTTAAAGAAGTATCAGAGTATTATCTAAAAGAT
GTTCCAAACGGTGAAAATATCGCACCTTTGCTTTCAGAGGGGCAAGAACTAATTGTTCAAATAGATAAAGAAGAACGAGG
CGATAAAGGTGCAGCATTAACTACATTTATTACCTTGGCTGGTTCGTATATGGTGTTATTGCCAAATAATCCTGAGGGGG
GTGGTATTTCCCGTCGTGTCGAAGGTGAAGATAGAGAAAGATTAAAAAAATATCTCAAAGAATTAAATATCCCAAAAAAT
ATGAGCGTAATAGCAAGAACTGCTTGCGTTGAGTGCTCTTTTGAAGAATTAAAACATGATTTTGATACTCTAGTTGAATT
ATGGGGATCTATTTCACAAGCTTACCATAGAATCAAAAAACCAGCTTTATTACATAAAGAAAGTGACATTATTGTAAGAA
CAGTTAGAGATCACTTAAAAGAAGATGTTAAAGAAATCATCGTTGATTCTAAAGAGTGCTTTGAAGATGTCAAAAGACAG
CTTAGTTTACTTAGACAAAGTTTTGATATAAACAAAGTAAAACTATACAACGAAGAATTACCATTATTTGCTCAGTTTGG
TATTGATCAACAAATAGAAAATGCTTATAAAAGAGAAATTCGCCTACCATCCGGCGGTTCTATCGTCATAGATACTACAG
AGGCACTTGTAGCAATAGATGTAAACTCATCTCGCGCTAACAAAGCCGAAGATGTAGAAACTACAGCATTTAAAACTAAC
CTAGAGGCTGCGGAAGAAGTTGCTCGTCAACTTAGAATTAGAGACTTAGGTGGTCTTGTTATAGTTGACTTTATTGATAT
GTCTTTCTACCCGAATCGAAAACAAGTCGAAGAAAAACTAATGGAAGCACTACAACAAGATAGAGCACGCATCCAAATGT
CACGCATTTCTAAACTTGGACTTGTTGAGATCTCAAGACAACGTTTAAGTTCTTCAATTAATGAAAGCGTAATGCAAAAA
TGCCCACGTTGTGAGGGTCATGGTTTTATCAAAACTACGCAAGCTACAGCACTTACCATCCTACGTAAAATCAGAGCAGA
AGCCATTAAAGAAGATACCAACGAAATTCGTGTCCAAGTTCCTGTAGACATCGCAGCATATATACTAAATGAAAAAAGAG
ATAGTATTGTTGAGATTGAGAGAATTTCTCAAGTTAAGGTTATGATTATTCCTAACTTTAATATGGAATCGCCAAAGTTT
CAAATGCAGAGAATCTGGGGTACTAGCTACAAATCAAATCGTACAAGCTCAGAATTAATAGAAGATGTTTATAATATTGA
AATTCCTAAAGCTGGTAAGAAAAAAATTGCGGCTGTGGATTTAAATCAAGCTATTGAAAATCAAGCTGCTAGCAACACTC
AAGAACAAAAGCCTCAAACTGATGCTCAAGTAGATATAGTTACACAAAATGATAAGAAACAAGATGATGTAACTCAACAA
GCTAAGAAAAAAGGCTTCTTTGCTAGGCTTTTTAGCGGTATATTTGCAACTGATTCAAAAGCTGCTCAGCAAGCTAATAA
CTCAGCTCAAAAACAACAAAACAAAGAACAGAATTTCCAAAGGCAGCAACAACATAATAAGGCGCAAAAAGATAATAATC
AACGTAATGAGCGTAACAATAGCAACAACAAAAATGAACGCAATAAAAATAGAAACAATAAAGGAGCAAAACCACAAATA
GCTCAAGAAAATAAACCACAAAAAGACAATAGCCAGCGTAGTGATCTCAATGCTAATGGTAACAGTAACAATGATAAAAA
TGAGCGCAATAAAAATAAATCAAACAATGATAAGTTTGATAAAAAATCAAATAACCGTAACAGCAGAAGCAATAATTTAA
ATCTAAATAATGCTGAAGAAGTTATTGATATTATGCAACTTAAGTATCAAAATCAAACAGCTATTGCAAAGACAGATAAT
ATCAAAAAGGTGATTTCGAAGAATCCTGATGAGTTTATCTCTGTAATGGTTAAAGATGTCTTAGAAAACTATGATAGTCT
AAAAGACGATGGTGCTACAAAGATAGCTACCCAGGAGAAAATTAAAACTAACAAATACTTAAAGTTTGATACTGTATCTG
TAGATACTGAAATAGCTCTACTTAATGAAGTCGCTAAATCAAATCAAACTGTTGGAGTTGAGCAAATCACAGCTGAAACA
GAAGAATCTTTAAGTATCGTTGATAAAGTTGATGACATAACAATACCAGAAGTTGTAGTAGAGCAAGAAAAGCCAGCGAA
AGTAGTTAAAGAAACTAATCAAACTAGTGAAGCTAAACCTAAAAAAGAAAAGCAAACTACTACAAAAAATAATAAGAAAG
AAAAACCAGCTGAAAAAGAGATGAAACAAAATCAAGCTAAACCTGAATATATAAACTATTCACCAGCGATTGATTTTGAA
TCTCAAGCACTAATATAG

Upstream 100 bases:

>100_bases
TCTCGCGATTTATTAAGAAAAGACCAAAATTGGTCACAAAGCGAGTTGGCTCGTTTAAAAGTCAGCTATGAGTTATATTT
ATTGAAGGAATTAATAACAA

Downstream 100 bases:

>100_bases
AGTAAGGTTTTACAATGCTACAATATCCTCATATTAATCCAGTTGCATTGCAGCTTGGTCCTATCAAGATTCATTGGTAT
GGTTTGATGTACCTACTTGG

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 885; Mature: 885

Protein sequence:

>885_residues
MKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNYGEEKNGFLPFKEVSEYYLKD
VPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLAGSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKN
MSVIARTACVECSFEELKHDFDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQ
LSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAIDVNSSRANKAEDVETTAFKTN
LEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKLMEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQK
CPRCEGHGFIKTTQATALTILRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKF
QMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQTDAQVDIVTQNDKKQDDVTQQ
AKKKGFFARLFSGIFATDSKAAQQANNSAQKQQNKEQNFQRQQQHNKAQKDNNQRNERNNSNNKNERNKNRNNKGAKPQI
AQENKPQKDNSQRSDLNANGNSNNDKNERNKNKSNNDKFDKKSNNRNSRSNNLNLNNAEEVIDIMQLKYQNQTAIAKTDN
IKKVISKNPDEFISVMVKDVLENYDSLKDDGATKIATQEKIKTNKYLKFDTVSVDTEIALLNEVAKSNQTVGVEQITAET
EESLSIVDKVDDITIPEVVVEQEKPAKVVKETNQTSEAKPKKEKQTTTKNNKKEKPAEKEMKQNQAKPEYINYSPAIDFE
SQALI

Sequences:

>Translated_885_residues
MKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNYGEEKNGFLPFKEVSEYYLKD
VPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLAGSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKN
MSVIARTACVECSFEELKHDFDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQ
LSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAIDVNSSRANKAEDVETTAFKTN
LEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKLMEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQK
CPRCEGHGFIKTTQATALTILRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKF
QMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQTDAQVDIVTQNDKKQDDVTQQ
AKKKGFFARLFSGIFATDSKAAQQANNSAQKQQNKEQNFQRQQQHNKAQKDNNQRNERNNSNNKNERNKNRNNKGAKPQI
AQENKPQKDNSQRSDLNANGNSNNDKNERNKNKSNNDKFDKKSNNRNSRSNNLNLNNAEEVIDIMQLKYQNQTAIAKTDN
IKKVISKNPDEFISVMVKDVLENYDSLKDDGATKIATQEKIKTNKYLKFDTVSVDTEIALLNEVAKSNQTVGVEQITAET
EESLSIVDKVDDITIPEVVVEQEKPAKVVKETNQTSEAKPKKEKQTTTKNNKKEKPAEKEMKQNQAKPEYINYSPAIDFE
SQALI
>Mature_885_residues
MKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNYGEEKNGFLPFKEVSEYYLKD
VPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLAGSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKN
MSVIARTACVECSFEELKHDFDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQ
LSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAIDVNSSRANKAEDVETTAFKTN
LEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKLMEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQK
CPRCEGHGFIKTTQATALTILRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKF
QMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQTDAQVDIVTQNDKKQDDVTQQ
AKKKGFFARLFSGIFATDSKAAQQANNSAQKQQNKEQNFQRQQQHNKAQKDNNQRNERNNSNNKNERNKNRNNKGAKPQI
AQENKPQKDNSQRSDLNANGNSNNDKNERNKNKSNNDKFDKKSNNRNSRSNNLNLNNAEEVIDIMQLKYQNQTAIAKTDN
IKKVISKNPDEFISVMVKDVLENYDSLKDDGATKIATQEKIKTNKYLKFDTVSVDTEIALLNEVAKSNQTVGVEQITAET
EESLSIVDKVDDITIPEVVVEQEKPAKVVKETNQTSEAKPKKEKQTTTKNNKKEKPAEKEMKQNQAKPEYINYSPAIDFE
SQALI

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=489, Percent_Identity=53.3742331288344, Blast_Score=547, Evalue=1e-156,
Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=33.9468302658487, Blast_Score=228, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 100687; Mature: 100687

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNY
CCEEEEECCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GEEKNGFLPFKEVSEYYLKDVPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLA
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
GSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKNMSVIARTACVECSFEELKHD
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHH
FDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQ
HHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAID
HHHHHHHCCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHEECCCCCEEEEECCCEEEEEE
VNSSRANKAEDVETTAFKTNLEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHH
MEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQKCPRCEGHGFIKTTQATALTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
LRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKF
HHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCCCHH
QMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQT
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHCCCCC
DAQVDIVTQNDKKQDDVTQQAKKKGFFARLFSGIFATDSKAAQQANNSAQKQQNKEQNFQ
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RQQQHNKAQKDNNQRNERNNSNNKNERNKNRNNKGAKPQIAQENKPQKDNSQRSDLNANG
HHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NSNNDKNERNKNKSNNDKFDKKSNNRNSRSNNLNLNNAEEVIDIMQLKYQNQTAIAKTDN
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECHHH
IKKVISKNPDEFISVMVKDVLENYDSLKDDGATKIATQEKIKTNKYLKFDTVSVDTEIAL
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHH
LNEVAKSNQTVGVEQITAETEESLSIVDKVDDITIPEVVVEQEKPAKVVKETNQTSEAKP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHCCCCCCCCC
KKEKQTTTKNNKKEKPAEKEMKQNQAKPEYINYSPAIDFESQALI
CHHHHHHCCCCCCCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNY
CCEEEEECCCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GEEKNGFLPFKEVSEYYLKDVPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLA
CCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
GSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKNMSVIARTACVECSFEELKHD
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHH
FDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQ
HHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAID
HHHHHHHCCCCEEEEECCCCCCHHHCCCCHHHHHHHHHHEECCCCCEEEEECCCEEEEEE
VNSSRANKAEDVETTAFKTNLEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCHHHHHHHH
MEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQKCPRCEGHGFIKTTQATALTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHH
LRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKF
HHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCCCHH
QMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQT
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHCCCCC
DAQVDIVTQNDKKQDDVTQQAKKKGFFARLFSGIFATDSKAAQQANNSAQKQQNKEQNFQ
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RQQQHNKAQKDNNQRNERNNSNNKNERNKNRNNKGAKPQIAQENKPQKDNSQRSDLNANG
HHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NSNNDKNERNKNKSNNDKFDKKSNNRNSRSNNLNLNNAEEVIDIMQLKYQNQTAIAKTDN
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECHHH
IKKVISKNPDEFISVMVKDVLENYDSLKDDGATKIATQEKIKTNKYLKFDTVSVDTEIAL
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEEECCHHHHH
LNEVAKSNQTVGVEQITAETEESLSIVDKVDDITIPEVVVEQEKPAKVVKETNQTSEAKP
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHCCCCCCCCC
KKEKQTTTKNNKKEKPAEKEMKQNQAKPEYINYSPAIDFESQALI
CHHHHHHCCCCCCCCCHHHHHHHCCCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]