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The map label for this gene is cspA [C]

Identifier: 134301709

GI number: 134301709

Start: 645990

End: 646193

Strand: Reverse

Name: cspA [C]

Synonym: FTW_0660

Alternate gene names: 134301709

Gene position: 646193-645990 (Counterclockwise)

Preceding gene: 134301711

Following gene: 134301707

Centisome position: 34.04

GC content: 34.8

Gene sequence:

>204_bases
ATGAGACAAGGTACAGTTAAATTTTTTAATACATCTAAAGGATTCGGATTCATAGAGCCTCAAGATGGTGGTAAAGATGT
ATTCGTTCATATCAGTGCAGTTGAAAATGCTGGTTTAAGCTCTCTACGTGAAGGCGAAAAAGTAAGCTTTGAAGTTGAAG
AAAACAGAGGCAAAATGGCTGCTGTTAATATAAAATCTATCTAA

Upstream 100 bases:

>100_bases
TTTCCAATATTCTATCCTTTATAAGTAAATCGTTAAAGTTTAAAACTAAGCCCAATGTGATTTAACTTATATAAACAACA
AAACAAAAGGAAATAAAACA

Downstream 100 bases:

>100_bases
TCTAATTATCCTTATATAACTGTAGGGACAGTCTTTCTTAAGAAAACAAATTCATATTTTTATTCTTCAATTAGCTATGA
AAAGATAGCCATTTCTTCGA

Product: cold shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 67

Protein sequence:

>67_residues
MRQGTVKFFNTSKGFGFIEPQDGGKDVFVHISAVENAGLSSLREGEKVSFEVEENRGKMAAVNIKSI

Sequences:

>Translated_67_residues
MRQGTVKFFNTSKGFGFIEPQDGGKDVFVHISAVENAGLSSLREGEKVSFEVEENRGKMAAVNIKSI
>Mature_67_residues
MRQGTVKFFNTSKGFGFIEPQDGGKDVFVHISAVENAGLSSLREGEKVSFEVEENRGKMAAVNIKSI

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=57.8125, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=53.8461538461538, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=49.2307692307692, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=54.0983606557377, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=46.875, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787107, Length=63, Percent_Identity=46.031746031746, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=46.875, Blast_Score=64, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24658520, Length=66, Percent_Identity=42.4242424242424, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7320; Mature: 7320

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQGTVKFFNTSKGFGFIEPQDGGKDVFVHISAVENAGLSSLREGEKVSFEVEENRGKMA
CCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEE
AVNIKSI
EEEEECC
>Mature Secondary Structure
MRQGTVKFFNTSKGFGFIEPQDGGKDVFVHISAVENAGLSSLREGEKVSFEVEENRGKMA
CCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCEE
AVNIKSI
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]