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The map label for this gene is ykfB [H]

Identifier: 134301694

GI number: 134301694

Start: 629074

End: 630051

Strand: Direct

Name: ykfB [H]

Synonym: FTW_0641

Alternate gene names: 134301694

Gene position: 629074-630051 (Clockwise)

Preceding gene: 134301693

Following gene: 134301695

Centisome position: 33.14

GC content: 33.95

Gene sequence:

>978_bases
ATGAATAAGATAATTGATATAAAAACATCTATAGTCACAATACCTCTTAAGGGAACTTTTGTAACAGCAGTGCGTAGTAC
TAATCATATCGATGCGGTGGTTGTTGAGCTGAGTTTAGATAATGGCAATAAGGGTTATGGTGTTGCGCCAGCTACAACTG
CTATCACTGGTGATACTTTACAAGGTATGCAATATATAATTAGTGAGATATTTGCTCCAGTTATACTTAGCTCAAATTTA
TCTGATTACAAACAAACTTTAGAACTAGCTTTTAAAAAAGTGATGTTTAATTCTGCTGCAAAAATGGCACTCGATTTAGC
TTTTCATGATTTGCTAGCTAAACAAAAAAATATCTCAGTAGCTAAACTACTTGGTGTAAAAAATAATATCATCGAAACTG
ATGTTTCTATAAGTTGTGGCAGTGTTGCAGAAACTATACAAAATATTCAAAATGGTGTGGAGGCTAATTTTACAACTATC
AAAGTCAAAACAGGTGCAGATTTTGATAGAGATATCCAATTGCTTAAAGCTCTTGATAATGAGTTTAGTAAAAATATCAA
ATTTAGGTTCGATGCTAATCAGGGTTGGAATATCTCGCAGACAAAGCAGTTTATCGAAGAGTTAAATAAATATAGCTTAA
ATGTTGAGATAATTGAGCAGCCAGTTAAATATTATGATATAAGTGCAATGCGTGAGATTACTAAATTTAGCAATATTCCT
ATCGTAGCAGATGAGTCAGTATTTGATGCCAAAGATGCAGAACGAGTTATAGATGAGCAAGCGTGTAATATGATTAATAT
TAAACTTGTTAAAAGCGGCGGTATCCTAGAGGCACAGAAAATCAAGAAATTAGCAGATAGAGCTGGTATATCATGTATGG
TGGGTTGTATGATGGAGTCACCAGCAGGTATTTTAGCTACAGCTAGTTTTGCATTAGCAGAAGGTATCACAGTAGCTGAT
CTTGATCCGTTAGATTGA

Upstream 100 bases:

>100_bases
GAAGTTTGGTTTCTATTGCTGTGGTTATGGCATTACTTGGCTTTTTGGTAGGTACCCAAGGTGGATTAATTGTAGCCAAA
ATACTTTCAGGATTTGCATT

Downstream 100 bases:

>100_bases
GTGGCCAAAGATTTGTATAGTGATTATATTACTTTTAATGAGCCTAATATTATCCTTAAAGATAATCTACAAGGATTTGG
TTTTAGTTTTTAGAAATATT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNKIIDIKTSIVTIPLKGTFVTAVRSTNHIDAVVVELSLDNGNKGYGVAPATTAITGDTLQGMQYIISEIFAPVILSSNL
SDYKQTLELAFKKVMFNSAAKMALDLAFHDLLAKQKNISVAKLLGVKNNIIETDVSISCGSVAETIQNIQNGVEANFTTI
KVKTGADFDRDIQLLKALDNEFSKNIKFRFDANQGWNISQTKQFIEELNKYSLNVEIIEQPVKYYDISAMREITKFSNIP
IVADESVFDAKDAERVIDEQACNMINIKLVKSGGILEAQKIKKLADRAGISCMVGCMMESPAGILATASFALAEGITVAD
LDPLD

Sequences:

>Translated_325_residues
MNKIIDIKTSIVTIPLKGTFVTAVRSTNHIDAVVVELSLDNGNKGYGVAPATTAITGDTLQGMQYIISEIFAPVILSSNL
SDYKQTLELAFKKVMFNSAAKMALDLAFHDLLAKQKNISVAKLLGVKNNIIETDVSISCGSVAETIQNIQNGVEANFTTI
KVKTGADFDRDIQLLKALDNEFSKNIKFRFDANQGWNISQTKQFIEELNKYSLNVEIIEQPVKYYDISAMREITKFSNIP
IVADESVFDAKDAERVIDEQACNMINIKLVKSGGILEAQKIKKLADRAGISCMVGCMMESPAGILATASFALAEGITVAD
LDPLD
>Mature_325_residues
MNKIIDIKTSIVTIPLKGTFVTAVRSTNHIDAVVVELSLDNGNKGYGVAPATTAITGDTLQGMQYIISEIFAPVILSSNL
SDYKQTLELAFKKVMFNSAAKMALDLAFHDLLAKQKNISVAKLLGVKNNIIETDVSISCGSVAETIQNIQNGVEANFTTI
KVKTGADFDRDIQLLKALDNEFSKNIKFRFDANQGWNISQTKQFIEELNKYSLNVEIIEQPVKYYDISAMREITKFSNIP
IVADESVFDAKDAERVIDEQACNMINIKLVKSGGILEAQKIKKLADRAGISCMVGCMMESPAGILATASFALAEGITVAD
LDPLD

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=244, Percent_Identity=29.0983606557377, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 35484; Mature: 35484

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIIDIKTSIVTIPLKGTFVTAVRSTNHIDAVVVELSLDNGNKGYGVAPATTAITGDTL
CCCEEEEEEEEEEEECCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCEECCHHH
QGMQYIISEIFAPVILSSNLSDYKQTLELAFKKVMFNSAAKMALDLAFHDLLAKQKNISV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AKLLGVKNNIIETDVSISCGSVAETIQNIQNGVEANFTTIKVKTGADFDRDIQLLKALDN
HHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
EFSKNIKFRFDANQGWNISQTKQFIEELNKYSLNVEIIEQPVKYYDISAMREITKFSNIP
HHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCC
IVADESVFDAKDAERVIDEQACNMINIKLVKSGGILEAQKIKKLADRAGISCMVGCMMES
EEECCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCC
PAGILATASFALAEGITVADLDPLD
CCCHHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure
MNKIIDIKTSIVTIPLKGTFVTAVRSTNHIDAVVVELSLDNGNKGYGVAPATTAITGDTL
CCCEEEEEEEEEEEECCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCEECCHHH
QGMQYIISEIFAPVILSSNLSDYKQTLELAFKKVMFNSAAKMALDLAFHDLLAKQKNISV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AKLLGVKNNIIETDVSISCGSVAETIQNIQNGVEANFTTIKVKTGADFDRDIQLLKALDN
HHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
EFSKNIKFRFDANQGWNISQTKQFIEELNKYSLNVEIIEQPVKYYDISAMREITKFSNIP
HHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCCC
IVADESVFDAKDAERVIDEQACNMINIKLVKSGGILEAQKIKKLADRAGISCMVGCMMES
EEECCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCC
PAGILATASFALAEGITVADLDPLD
CCCHHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]