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The map label for this gene is gcvH1 [H]

Identifier: 134301669

GI number: 134301669

Start: 603140

End: 603520

Strand: Direct

Name: gcvH1 [H]

Synonym: FTW_0609

Alternate gene names: 134301669

Gene position: 603140-603520 (Clockwise)

Preceding gene: 134301665

Following gene: 134301670

Centisome position: 31.77

GC content: 31.76

Gene sequence:

>381_bases
ATGGCTGAAATAAATCAAAATTACTTATATACAGATTCGAATTTATGGGTTGAGGTCAAAGGTAATATTGCAAGAGTTGG
TATAGATGATTACTCCCAAGATGAATTTGGAGAAATTGTCTATGTTGATTTGCCTAAACTTGAACAAGAGTATGCCAAAG
ATGATGAGATTTGTGTTATAGAATCTGTAAAGACCGCATCTGATATTTATACACCTCTATCTGGGAAAGTTGTAAAAATA
AATCAACAATTATTAGATAATCCAAAGCTTATAAACGAGTCTTGTTATGATAAGGGTTGGCTATTTGAAATTGAAATAGC
TGATCTAGATGAGCTTAAGAGCTTAATAAATCCTCAAGACTATCAAAGCTATGTTAGCTAG

Upstream 100 bases:

>100_bases
TAGATTATCAACTTTGTTTTACAAATTAATTTTAAAGGATTTTTATGTTATTATCATCTATTAGCTAAAATAAAAATAGG
GTTTTAATCCAAGAGGAATT

Downstream 100 bases:

>100_bases
AGTTTTATTAATTCATAGCTTAATTTTTTTATCATCATCTGCTTTTGCTCTAGAGCAATATCTTAATTATTGTAAGATAT
ATAATCCAAAGAAAGGTATC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MAEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVIESVKTASDIYTPLSGKVVKI
NQQLLDNPKLINESCYDKGWLFEIEIADLDELKSLINPQDYQSYVS

Sequences:

>Translated_126_residues
MAEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVIESVKTASDIYTPLSGKVVKI
NQQLLDNPKLINESCYDKGWLFEIEIADLDELKSLINPQDYQSYVS
>Mature_125_residues
AEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVIESVKTASDIYTPLSGKVVKIN
QQLLDNPKLINESCYDKGWLFEIEIADLDELKSLINPQDYQSYVS

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=39.6551724137931, Blast_Score=111, Evalue=2e-25,
Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=38.7931034482759, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=36.2903225806452, Blast_Score=96, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=38.3333333333333, Blast_Score=104, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=38.9830508474576, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=33.8842975206612, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=43.2203389830509, Blast_Score=115, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14479; Mature: 14348

Theoretical pI: Translated: 3.84; Mature: 3.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVI
CCCCCCCEEEECCCEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHCCCCCEEEE
ESVKTASDIYTPLSGKVVKINQQLLDNPKLINESCYDKGWLFEIEIADLDELKSLINPQD
ECCCCHHHHCCCCCCEEEEECHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCH
YQSYVS
HHHHCC
>Mature Secondary Structure 
AEINQNYLYTDSNLWVEVKGNIARVGIDDYSQDEFGEIVYVDLPKLEQEYAKDDEICVI
CCCCCCEEEECCCEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHCCCCCEEEE
ESVKTASDIYTPLSGKVVKINQQLLDNPKLINESCYDKGWLFEIEIADLDELKSLINPQD
ECCCCHHHHCCCCCCEEEEECHHHCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCH
YQSYVS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA