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The map label for this gene is fabI [H]

Identifier: 134301639

GI number: 134301639

Start: 572577

End: 573359

Strand: Direct

Name: fabI [H]

Synonym: FTW_0579

Alternate gene names: 134301639

Gene position: 572577-573359 (Clockwise)

Preceding gene: 134301636

Following gene: 134301640

Centisome position: 30.16

GC content: 37.04

Gene sequence:

>783_bases
ATGGGTTTTCTAGCAGGAAAAAAAATATTAATCACTGGACTTTTAAGTAATAAGTCAATTGCATATGGTATTGCTAAAGC
TATGCATAGAGAGGGAGCCGAGCTTGCTTTTACTTATGTTGGACAGTTCAAAGATAGAGTGGAAAAATTATGTGCAGAAT
TTAATCCAGCTGCAGTTTTGCCTTGCGATGTGATTTCTGATCAAGAGATTAAGGATTTATTTGTAGAGCTAGGTAAAGTT
TGGGATGGTCTAGATGCCATAGTTCATTCTATAGCTTTTGCACCGCGTGATCAGTTAGAAGGTAACTTTATTGACTGTGT
AACTCGCGAGGGTTTTAGTATCGCTCATGATATTAGTGCCTATTCTTTTGCAGCATTAGCTAAAGAAGGTCGTAGTATGA
TGAAAAATCGTAATGCTTCTATGGTAGCACTTACTTATATTGGAGCAGAAAAAGCTATGCCAAGTTACAATACTATGGGT
GTTGCTAAAGCATCTCTAGAAGCTACAGTTAGATATACAGCGTTAGCTTTAGGTGAGGATGGTATCAAGGTAAATGCTGT
ATCAGCTGGTCCTATCAAAACTCTGGCAGCTTCTGGTATATCAAACTTCAAGAAGATGCTTGATTATAATGCTATGGTTT
CTCCACTTAAGAAAAATGTTGATATTATGGAAGTTGGTAATACTGTAGCGTTTTTATGTTCAGATATGGCAACTGGTATC
ACTGGAGAAGTTGTCCATGTTGATGCTGGATATCATTGTGTGTCTATGGGTAATGTTCTTTAA

Upstream 100 bases:

>100_bases
GTTAGCTTTGTAATTTAAAAATAAAAGAGCGTTGTATAACTATTTATGAAATTATTAGTAACTAAGATTTTAACAATAAA
TCCACAAAGGAGATGTAAAT

Downstream 100 bases:

>100_bases
TTTTCTAAATTAATCTGTTTCCAATCAATTAGATTTATTTGTATCTATATAATAAAAACTGATATATTTTATAAAAAATA
TTAGTTTTTGTTATCTATTT

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVLPCDVISDQEIKDLFVELGKV
WDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISAYSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMG
VAKASLEATVRYTALALGEDGIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
TGEVVHVDAGYHCVSMGNVL

Sequences:

>Translated_260_residues
MGFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVLPCDVISDQEIKDLFVELGKV
WDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISAYSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMG
VAKASLEATVRYTALALGEDGIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
TGEVVHVDAGYHCVSMGNVL
>Mature_259_residues
GFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVLPCDVISDQEIKDLFVELGKVW
DGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISAYSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMGV
AKASLEATVRYTALALGEDGIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGIT
GEVVHVDAGYHCVSMGNVL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=59.1439688715953, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=28.125, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27806; Mature: 27674

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVL
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEE
PCDVISDQEIKDLFVELGKVWDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHH
YSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMGVAKASLEATVRYTALALGED
HHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCCCHHHHHHHHHHHEEEEEEECCC
GIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCC
TGEVVHVDAGYHCVSMGNVL
CCEEEEEECCCEEEECCCCC
>Mature Secondary Structure 
GFLAGKKILITGLLSNKSIAYGIAKAMHREGAELAFTYVGQFKDRVEKLCAEFNPAAVL
CCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCEEE
PCDVISDQEIKDLFVELGKVWDGLDAIVHSIAFAPRDQLEGNFIDCVTREGFSIAHDISA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCCCHHHHHHH
YSFAALAKEGRSMMKNRNASMVALTYIGAEKAMPSYNTMGVAKASLEATVRYTALALGED
HHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCCCHHHHHHHHHHHEEEEEEECCC
GIKVNAVSAGPIKTLAASGISNFKKMLDYNAMVSPLKKNVDIMEVGNTVAFLCSDMATGI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHCCC
TGEVVHVDAGYHCVSMGNVL
CCEEEEEECCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10464225; 10984043 [H]