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The map label for this gene is trmE [H]

Identifier: 134301618

GI number: 134301618

Start: 555130

End: 556482

Strand: Reverse

Name: trmE [H]

Synonym: FTW_0557

Alternate gene names: 134301618

Gene position: 556482-555130 (Counterclockwise)

Preceding gene: 134301619

Following gene: 134301615

Centisome position: 29.31

GC content: 32.08

Gene sequence:

>1353_bases
ATGTACACAAAAGATACAATAGTTGCAATAGCAACTCCTCAAGGTAATGGCGGTATAGGCATAATCCGAATATCTGGGAT
AGATGCTCTAGCAATAGCTGAGAAACTAACTAAAAAACAGCTAAAACCTCGTTATGCAACATTCTGTAATGTCTATAATG
ATAATGAAATAATAGATCATGGTATCGTAATATTCTTTAAGGCGCCTTTGTCATATACAGGTGAAGACGTTGTAGAAATT
CAAGCTCATGGTAACCCATTTATATTGAATTTGATTATCAAAGCAGCACTTAACTGTGGTGCCAGAATGGCAAAGGCAGG
TGAATTTACTGAGAGAGCATTTCTGAATAATAAACTTGATTTAGCTCAAGCAGAAGCTGTTGCTGATATTATTAATGCAT
CATCAGAAATAGCAGCTAAGTCGGCAGCTAAATCACTTCAAGGAGATTTCTCTAAAGAAATAAATAATCTTTTAGAAAAA
CTTATTTATCTAAGAATGTATGTAGAAGCGTCTATTGACTTTCCAGAAGAAGAAATAAACTTTTTAGAAGATCAAAAAAT
TCATTCTAGTTTAGAAGAAATATATAAGGTTATCTTAGCGGTAAAAAATAGCTGTAAACAAGGTGTTATTCTTGCTGAGG
GAATTACATTAATATTAGTAGGCAAACCAAATGCTGGTAAGTCAAGCCTTTTGAATGCTCTTGCAGGTAAAGAATCAGCT
ATTGTAACATCAATAGCAGGTACAACTAGAGATATTGTCAAAGAACATATTCAAATAAATGGCGTTCCTATGCATATCAT
TGACACTGCAGGGCTACGTAATAGTGATGATATCATCGAAAGTGAAGGAATCAAAAGAGCTATTAAGAAAATTCAAGAAG
CTGATCAAGTACTATTTGTAACTGATGACTATACGAATAGCCAAGTTAAATTTAGTGATATAAAAGAGATAATTCCAGAA
TTTTATGATCAAATTCCTAAAGATATCGATATTACATATGTACATAATAAGATAGATCTCCTTAAAGAAGTTCCACACAA
TCATGCTAACCACATATACATATCAGCCGAAAATAACATTGGTATTGATAAACTAAAAGAACATATTCTCAATAAAGTTG
GTTATACAAATCAAAATGAAAGTATTTATACTGCACGTGAAAGACATGTTACAGCGATAAATAATGCTTTTGAGCATATT
AAACTTGCTAGAGAACAATTAGAACTTGGTAATGGTGAGCTTCTAGCTGAAGAGTTGTTAATTGTTCAAGAATATCTTAA
TTCAATTACAGGTGAGTTTAGTTCTGATGACTTATTAGGTGAAATATTCTCAAGCTTTTGTATCGGCAAATAA

Upstream 100 bases:

>100_bases
ATCCAGAATTTTTTAGACAATATAAGAGTAAAAAAGGTAGAGTTATTGATCAAGAAAATAATGATTTTGATAGTTCAAAT
CAAAAGTTAAGATAGATAAT

Downstream 100 bases:

>100_bases
ATTGTCTAGCTATTTATTAGCAACTGCTATTTTATCTTCTAGTTGCTGAACCCTTGATGATTCTTGTTGTTCGTTATTTC
TAGCTTTTGACGTGTTATTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MYTKDTIVAIATPQGNGGIGIIRISGIDALAIAEKLTKKQLKPRYATFCNVYNDNEIIDHGIVIFFKAPLSYTGEDVVEI
QAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLDLAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEK
LIYLRMYVEASIDFPEEEINFLEDQKIHSSLEEIYKVILAVKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFVTDDYTNSQVKFSDIKEIIPE
FYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNIGIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHI
KLAREQLELGNGELLAEELLIVQEYLNSITGEFSSDDLLGEIFSSFCIGK

Sequences:

>Translated_450_residues
MYTKDTIVAIATPQGNGGIGIIRISGIDALAIAEKLTKKQLKPRYATFCNVYNDNEIIDHGIVIFFKAPLSYTGEDVVEI
QAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLDLAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEK
LIYLRMYVEASIDFPEEEINFLEDQKIHSSLEEIYKVILAVKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFVTDDYTNSQVKFSDIKEIIPE
FYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNIGIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHI
KLAREQLELGNGELLAEELLIVQEYLNSITGEFSSDDLLGEIFSSFCIGK
>Mature_450_residues
MYTKDTIVAIATPQGNGGIGIIRISGIDALAIAEKLTKKQLKPRYATFCNVYNDNEIIDHGIVIFFKAPLSYTGEDVVEI
QAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLDLAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEK
LIYLRMYVEASIDFPEEEINFLEDQKIHSSLEEIYKVILAVKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFVTDDYTNSQVKFSDIKEIIPE
FYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNIGIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHI
KLAREQLELGNGELLAEELLIVQEYLNSITGEFSSDDLLGEIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=31.3432835820896, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=29.4589178356713, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI193082993, Length=466, Percent_Identity=30.4721030042918, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI2367268, Length=459, Percent_Identity=52.5054466230937, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI87082120, Length=84, Percent_Identity=39.2857142857143, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=29.4372294372294, Blast_Score=175, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=504, Percent_Identity=29.7619047619048, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45550813, Length=476, Percent_Identity=30.4621848739496, Blast_Score=188, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49796; Mature: 49796

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTKDTIVAIATPQGNGGIGIIRISGIDALAIAEKLTKKQLKPRYATFCNVYNDNEIIDH
CCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHEECCCCEEECC
GIVIFFKAPLSYTGEDVVEIQAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLD
CEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHCCCCH
LAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEKLIYLRMYVEASIDFPEEEIN
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FLEDQKIHSSLEEIYKVILAVKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHCCCCC
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFV
EEEHHCCCHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TDDYTNSQVKFSDIKEIIPEFYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNI
ECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCC
GIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHIKLAREQLELGNGELLAEELL
CHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IVQEYLNSITGEFSSDDLLGEIFSSFCIGK
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MYTKDTIVAIATPQGNGGIGIIRISGIDALAIAEKLTKKQLKPRYATFCNVYNDNEIIDH
CCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHEECCCCEEECC
GIVIFFKAPLSYTGEDVVEIQAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLD
CEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHCCCCH
LAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEKLIYLRMYVEASIDFPEEEIN
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FLEDQKIHSSLEEIYKVILAVKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHCCCCC
IVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFV
EEEHHCCCHHHHHHHHHEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
TDDYTNSQVKFSDIKEIIPEFYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNI
ECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCEEEEEECCCC
GIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHIKLAREQLELGNGELLAEELL
CHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IVQEYLNSITGEFSSDDLLGEIFSSFCIGK
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA