Click here to switch to the map view.

The map label for this gene is 134301615

Identifier: 134301615

GI number: 134301615

Start: 553024

End: 553869

Strand: Reverse

Name: 134301615

Synonym: FTW_0554

Alternate gene names: NA

Gene position: 553869-553024 (Counterclockwise)

Preceding gene: 134301618

Following gene: 134301614

Centisome position: 29.17

GC content: 32.03

Gene sequence:

>846_bases
ATGAATAAAATTATCTCAAGGGTTGTATTAACTGGCTGTATCTTTAGTATAGCTTCAGCTACTTTTGCTTGCTCAGAATT
TAATCATAACTTTGGTGGCGATTTAGGTGTCTATACTACTAGAACGATGGATTTATTTATAGATTTAAAACCAAATTTGA
CTATATATCCAAGAGGTACAAAAGAAACAGGAGGATTAAAAAATAATTCTCTTACATGGACTGATAAATATGGCTATGTA
TCTATTGATGAAACAAATTTAGCTAATTTAACTGGTGAAGGTCTTAATGAAAAAGGCTTAGTAGCACATCTACTTTATCT
TGGGGATACTATTCAGCCAAAAAGAGATACAAGCAAGCCAGGTGTTAATGGATTATATTGGATTAGGTATGTATTAGGTA
ATTTTGCTACAGTTAAGGAAGTTCTTGACAATCTTGATAGCTATCAAATATATAACCCTCCGATAGAAATTAATGGTAAA
GAGAGCAATATCCCAGTTCACTACATGGTTGAAGATAAAACAGGAGATAGTGCACTAATTGAGTATATAAATGGTAAATT
AACTGTACATAGAAATGTTAAAGCAATTAGCAATGAACCAAGCTATGATGAACAGCAAAAAATCCTTAGGCAAGCTAAAG
AGCTGGGGTTTTATAATATTGATAAATTACCTGGTGGAGCAAATTCTGCTTACAGATTCGTTAGAACAACTTTTATAAGT
GAAAATCTTCCAAAAGCAAAATCGATGAATCAAGCTGTCAATTATATGTTTGCAGCTGCTGACTCAGTTTCTGTACCTTT
CATAAAAGGTTATAGAAATGAAAGTTTAAACAACCCAAGTCTATAA

Upstream 100 bases:

>100_bases
TTTTTGCTACAGACAAATTAATACTACTACAAATATACTATTAGCGTAAGTTAATTGACTAATTTTAAAATTATTCAATA
TAAGTAGATAAGGAAGGAAT

Downstream 100 bases:

>100_bases
GATAAATGGCCAACACAATGGAAATCAGTAATATCATTATCACAAAATAGACTATATGTAGTAGATACTCTTGTAGGCAA
TAGAGTATATGTTAACTTAA

Product: choloylglycine hydrolase family protein

Products: NA

Alternate protein names: Penicillin V amidase; PVA [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNKIISRVVLTGCIFSIASATFACSEFNHNFGGDLGVYTTRTMDLFIDLKPNLTIYPRGTKETGGLKNNSLTWTDKYGYV
SIDETNLANLTGEGLNEKGLVAHLLYLGDTIQPKRDTSKPGVNGLYWIRYVLGNFATVKEVLDNLDSYQIYNPPIEINGK
ESNIPVHYMVEDKTGDSALIEYINGKLTVHRNVKAISNEPSYDEQQKILRQAKELGFYNIDKLPGGANSAYRFVRTTFIS
ENLPKAKSMNQAVNYMFAAADSVSVPFIKGYRNESLNNPSL

Sequences:

>Translated_281_residues
MNKIISRVVLTGCIFSIASATFACSEFNHNFGGDLGVYTTRTMDLFIDLKPNLTIYPRGTKETGGLKNNSLTWTDKYGYV
SIDETNLANLTGEGLNEKGLVAHLLYLGDTIQPKRDTSKPGVNGLYWIRYVLGNFATVKEVLDNLDSYQIYNPPIEINGK
ESNIPVHYMVEDKTGDSALIEYINGKLTVHRNVKAISNEPSYDEQQKILRQAKELGFYNIDKLPGGANSAYRFVRTTFIS
ENLPKAKSMNQAVNYMFAAADSVSVPFIKGYRNESLNNPSL
>Mature_281_residues
MNKIISRVVLTGCIFSIASATFACSEFNHNFGGDLGVYTTRTMDLFIDLKPNLTIYPRGTKETGGLKNNSLTWTDKYGYV
SIDETNLANLTGEGLNEKGLVAHLLYLGDTIQPKRDTSKPGVNGLYWIRYVLGNFATVKEVLDNLDSYQIYNPPIEINGK
ESNIPVHYMVEDKTGDSALIEYINGKLTVHRNVKAISNEPSYDEQQKILRQAKELGFYNIDKLPGGANSAYRFVRTTFIS
ENLPKAKSMNQAVNYMFAAADSVSVPFIKGYRNESLNNPSL

Specific function: The enzyme catalyzes the conversion of penicillin to 6- aminopenicillanate The precursor, furthermore, acts as a self- processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase [H]

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: =3.5.1.11 [H]

Molecular weight: Translated: 31336; Mature: 31336

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIISRVVLTGCIFSIASATFACSEFNHNFGGDLGVYTTRTMDLFIDLKPNLTIYPRGT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCEEEEECCC
KETGGLKNNSLTWTDKYGYVSIDETNLANLTGEGLNEKGLVAHLLYLGDTIQPKRDTSKP
CCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
GVNGLYWIRYVLGNFATVKEVLDNLDSYQIYNPPIEINGKESNIPVHYMVEDKTGDSALI
CCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCEEECCCCCCCCEEEEEECCCCCHHHH
EYINGKLTVHRNVKAISNEPSYDEQQKILRQAKELGFYNIDKLPGGANSAYRFVRTTFIS
HHHCCEEEEEECEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHH
ENLPKAKSMNQAVNYMFAAADSVSVPFIKGYRNESLNNPSL
CCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNKIISRVVLTGCIFSIASATFACSEFNHNFGGDLGVYTTRTMDLFIDLKPNLTIYPRGT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCEEEEECCC
KETGGLKNNSLTWTDKYGYVSIDETNLANLTGEGLNEKGLVAHLLYLGDTIQPKRDTSKP
CCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
GVNGLYWIRYVLGNFATVKEVLDNLDSYQIYNPPIEINGKESNIPVHYMVEDKTGDSALI
CCCHHHHHHHHHCCHHHHHHHHHCCCCCEEECCCEEECCCCCCCCEEEEEECCCCCHHHH
EYINGKLTVHRNVKAISNEPSYDEQQKILRQAKELGFYNIDKLPGGANSAYRFVRTTFIS
HHHCCEEEEEECEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHH
ENLPKAKSMNQAVNYMFAAADSVSVPFIKGYRNESLNNPSL
CCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026906 [H]