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The map label for this gene is sun [H]

Identifier: 134301576

GI number: 134301576

Start: 495970

End: 497250

Strand: Reverse

Name: sun [H]

Synonym: FTW_0506

Alternate gene names: 134301576

Gene position: 497250-495970 (Counterclockwise)

Preceding gene: 134301577

Following gene: 134301571

Centisome position: 26.19

GC content: 30.21

Gene sequence:

>1281_bases
ATGAATACCCGCGCTATTGCAGCTAAAACTATTTTAGATATTCTCGATAACAAATATTCTCTACTAACTATTGAGCAAAA
ACTTGTTAAGCTTAACCTATTAGAGCAAGATAAATCTTTTATAAAACTTTTATGTTATGAATTTTTCAGACATTACTACT
CGTTAGAAAAGATTATCGCTTTATATCTGACACAAAAAACTAAGGTAAAAGCTAAAGTTTTAATAATGCTTGGAGCATTA
CAGATATTTGAAATCAAACAACCTTACTATGCAACTATTAATGAAACTGTAGCAGCTTGTAAAGTTCTCAAAATTATCTG
GGCAAAGAAACTAGTTAATCGAATACTAAGAGAAATGACTCGTAATATCGAATATATAACTTCTATCTATACTCAAAATA
AAGCAATAGATATGCCTGAATGGCTTTGTGATACTCTCAAAAAGCAATATCCTAACGATCATCTAGTGATAGCTCAAGCA
ATCAATGCAAAAGCTGATATGTTTATTCGTTTAAACCAAAACAAAGATAATAAAAAAGTCTTAGATTATTTTGACAAAAA
TAACATAGGATATTCTTTTACAAACCTAAAAGATTGTATAAAACTTGATCAAGCTATCGATGTAAAATACAATCAGCTTT
TTCAACAAGGCTATTTTACCATACAGGATATCTCTGCACAGTATGCTGGTCATATTATCAGAGCTGCAAATAATGATAAA
ATTCTTGATGCTTGTGCTGCTCCTGGGGGTAAAACTTCACATATTTTAGAATGCGCTCCGCAAGCGGATATTACTGCTAT
AGATATTATTGATAAGCGTTTAGAACTTCTTAGAGAAAACTTAGAGAGATTATCTGAAAATCATAATGTCAATGTTTTTA
AACATGATTTGACTCTACCTCTAGCCGGAAAATTTAATAAGATTATCCTTGATGCGCCATGCTCAGCCTTAGGAACTATA
AGACGAAATCCCGACATCAAAGTGCTAAGAACACCGAATGATATTCAAGCTATCAAAAGCTTACAAAGTAAAATTTTGGC
TAACTTATGGAATAATAACCTTACTGATAATGGTTATTTACTCTATATTACATGTTCAATACTAGCAGAAGAAAATCAAC
AACAAATAAAAGAGTTTTTAGCTAAAAATAATAATGCTGAGATTATTGAGATAGATATCCTTGAAAAATATAAAACTAGT
TATGGCTATCAATTACTGCCATCTCAACAAGATGGTGATGGCTTTTATTACTGTTTACTTAAAAAAAATCCTTATTTTTA
G

Upstream 100 bases:

>100_bases
ACATCCATGGTACTAGTGACCTTGACTCTGGTTTGATCGTTGCTGGTAATAAAAAAATCCAACCAAAACTTATTAAAATT
CTAGCAAAACATATCAAATA

Downstream 100 bases:

>100_bases
CAGCAATTTTTTCTATAATTTCTTGAGGTGTTGCAGAAAGTTTTAACTTATTTATATGGTACTGCGAGGAGAAAATAATA
TCATCTATTTTATCTTCAAT

Product: ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIALYLTQKTKVKAKVLIMLGAL
QIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMTRNIEYITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIAQA
INAKADMFIRLNQNKDNKKVLDYFDKNNIGYSFTNLKDCIKLDQAIDVKYNQLFQQGYFTIQDISAQYAGHIIRAANNDK
ILDACAAPGGKTSHILECAPQADITAIDIIDKRLELLRENLERLSENHNVNVFKHDLTLPLAGKFNKIILDAPCSALGTI
RRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYLLYITCSILAEENQQQIKEFLAKNNNAEIIEIDILEKYKTS
YGYQLLPSQQDGDGFYYCLLKKNPYF

Sequences:

>Translated_426_residues
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIALYLTQKTKVKAKVLIMLGAL
QIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMTRNIEYITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIAQA
INAKADMFIRLNQNKDNKKVLDYFDKNNIGYSFTNLKDCIKLDQAIDVKYNQLFQQGYFTIQDISAQYAGHIIRAANNDK
ILDACAAPGGKTSHILECAPQADITAIDIIDKRLELLRENLERLSENHNVNVFKHDLTLPLAGKFNKIILDAPCSALGTI
RRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYLLYITCSILAEENQQQIKEFLAKNNNAEIIEIDILEKYKTS
YGYQLLPSQQDGDGFYYCLLKKNPYF
>Mature_426_residues
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIALYLTQKTKVKAKVLIMLGAL
QIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMTRNIEYITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIAQA
INAKADMFIRLNQNKDNKKVLDYFDKNNIGYSFTNLKDCIKLDQAIDVKYNQLFQQGYFTIQDISAQYAGHIIRAANNDK
ILDACAAPGGKTSHILECAPQADITAIDIIDKRLELLRENLERLSENHNVNVFKHDLTLPLAGKFNKIILDAPCSALGTI
RRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYLLYITCSILAEENQQQIKEFLAKNNNAEIIEIDILEKYKTS
YGYQLLPSQQDGDGFYYCLLKKNPYF

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=187, Percent_Identity=31.0160427807487, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI76150623, Length=187, Percent_Identity=31.0160427807487, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI32698918, Length=181, Percent_Identity=29.8342541436464, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI8922322, Length=249, Percent_Identity=25.7028112449799, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI270288816, Length=249, Percent_Identity=25.7028112449799, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI270288818, Length=332, Percent_Identity=23.7951807228916, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI23199998, Length=249, Percent_Identity=25.7028112449799, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI39995082, Length=202, Percent_Identity=29.2079207920792, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI301336155, Length=202, Percent_Identity=29.2079207920792, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI11545785, Length=191, Percent_Identity=29.8429319371728, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367212, Length=431, Percent_Identity=35.4988399071926, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI87081985, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17536757, Length=239, Percent_Identity=28.0334728033473, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=239, Percent_Identity=26.7782426778243, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319447, Length=188, Percent_Identity=30.3191489361702, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI22024126, Length=229, Percent_Identity=27.5109170305677, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355201, Length=249, Percent_Identity=24.8995983935743, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49101; Mature: 49101

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIA
CCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLTQKTKVKAKVLIMLGALQIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMT
HHHCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIEYITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIAQAINAKADMFIRLNQNKDNKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHHH
LDYFDKNNIGYSFTNLKDCIKLDQAIDVKYNQLFQQGYFTIQDISAQYAGHIIRAANNDK
HHHHCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEHHHHHHCCEEEEECCCCC
ILDACAAPGGKTSHILECAPQADITAIDIIDKRLELLRENLERLSENHNVNVFKHDLTLP
CHHHHCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECEE
LAGKFNKIILDAPCSALGTIRRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYL
CCCCCCEEEEECCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LYITCSILAEENQQQIKEFLAKNNNAEIIEIDILEKYKTSYGYQLLPSQQDGDGFYYCLL
EEEEEEEHHCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCEEECCCCCCCCEEEEEEE
KKNPYF
ECCCCC
>Mature Secondary Structure
MNTRAIAAKTILDILDNKYSLLTIEQKLVKLNLLEQDKSFIKLLCYEFFRHYYSLEKIIA
CCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLTQKTKVKAKVLIMLGALQIFEIKQPYYATINETVAACKVLKIIWAKKLVNRILREMT
HHHCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNIEYITSIYTQNKAIDMPEWLCDTLKKQYPNDHLVIAQAINAKADMFIRLNQNKDNKKV
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCCHHH
LDYFDKNNIGYSFTNLKDCIKLDQAIDVKYNQLFQQGYFTIQDISAQYAGHIIRAANNDK
HHHHCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHCCEEEEHHHHHHCCEEEEECCCCC
ILDACAAPGGKTSHILECAPQADITAIDIIDKRLELLRENLERLSENHNVNVFKHDLTLP
CHHHHCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECEE
LAGKFNKIILDAPCSALGTIRRNPDIKVLRTPNDIQAIKSLQSKILANLWNNNLTDNGYL
CCCCCCEEEEECCCHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LYITCSILAEENQQQIKEFLAKNNNAEIIEIDILEKYKTSYGYQLLPSQQDGDGFYYCLL
EEEEEEEHHCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCEEECCCCCCCCEEEEEEE
KKNPYF
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]