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The map label for this gene is wbtI [H]

Identifier: 134301501

GI number: 134301501

Start: 418962

End: 420044

Strand: Direct

Name: wbtI [H]

Synonym: FTW_0420

Alternate gene names: 134301501

Gene position: 418962-420044 (Clockwise)

Preceding gene: 134301500

Following gene: 134301502

Centisome position: 22.07

GC content: 30.66

Gene sequence:

>1083_bases
ATGAGTAAAGTAAATGTAACAAAACCATACTTACCAGATATAAATAAATATAAAAGCTATGTAAATAAAATATACAAAAA
TGGATGGCTTACTAATAATGGTCCGTTAGTGCAAGAGCTAGAAAAAAGACTTGCAAAGTATCTAGGTGTTAAAAATATAG
TTTTAGTATCAAATGGTACAATTGCATTAGAAATCGCGTATAGAGCGTTAGGAGTCAAAGGAAGTGCAATTACTACTCCA
TTTTCATTTGTTGCTACTACATCTTCATTGGTTTCTAACAATGTAAAACCAGTGTTTGTTGATATTGATGAGAATACTCT
AAGTATAGACGTCTCTAAAATTAAGTATGCTATTGAAGAGGATACTTCAGCTATTGTGCCAGTTCATGTGTTTGGAAATG
GTTGTGAAGTTGAAAAAATAGACATGCTGGCTAAAAAACATAACTTAAAAGTTATTTATGATGCAGCACATGCTTTTGAT
GTTAAGTATAAGGGTGAGAGTATATTAAACTATGGTGATATTTCGACATTAAGTTTTCATGCAACAAAGATTTTTCATTC
TATTGAAGGAGGTGCGCTTATCATTAATGATGATAGTCTTGTTGAAAAAGTTCGTTATTTCATTAATTTTGGTATAGAAA
GCTCAGAATCAATACCTTACTTAGGTACTAATGCTAAAATGAATGAATTTGAGGCGGCTATGGGACTTTGTGTTCTAGAT
GATATTATAGAAATTAAGAGCAAAAGGAAAGTTATTACAGAGATATATGAGGCTGGGTTAGATGGATTGGTAAAGTTTCA
AGAACAGAATCAGCATTCTAGTAGGAATTATAGCTATTTTCCAGTAATATTTAGGACTGAGGAGGAACTTCTCAGAGTAC
AGAAAGCACTAATACAAAATGATATAATATCGCGTAGATATTTTTATCCATCATTAGATAGTCTTAGTTATATAGAGCCA
AAGCAGTATATGCCAATCTCAAGAGATATATCTAAAAGAATATTATGTTTGCCAATTTATGCAGAGTTAGAAGACGATAA
AATTAATAAAATAATTAATAATATCAAAGAGGTTTCCTCATGA

Upstream 100 bases:

>100_bases
CCTGAGGTGGTGCAAAAATATTGGCAAGAGCATTTGAGTGGTAAAAGAAATTGGCAAGCAATATTATGGAATATTCTAAT
TTTTCAGGAGTGGTTAGATA

Downstream 100 bases:

>100_bases
AAAAAATATTTGTTGTTACAGATAATAGAACTATTCTAAGTGATTTTAAAAATATCATTGGTAGTAAAAATGATGTAGAG
GTTGATTATTTTTGTAGTTT

Product: aminotransferase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSKVNVTKPYLPDINKYKSYVNKIYKNGWLTNNGPLVQELEKRLAKYLGVKNIVLVSNGTIALEIAYRALGVKGSAITTP
FSFVATTSSLVSNNVKPVFVDIDENTLSIDVSKIKYAIEEDTSAIVPVHVFGNGCEVEKIDMLAKKHNLKVIYDAAHAFD
VKYKGESILNYGDISTLSFHATKIFHSIEGGALIINDDSLVEKVRYFINFGIESSESIPYLGTNAKMNEFEAAMGLCVLD
DIIEIKSKRKVITEIYEAGLDGLVKFQEQNQHSSRNYSYFPVIFRTEEELLRVQKALIQNDIISRRYFYPSLDSLSYIEP
KQYMPISRDISKRILCLPIYAELEDDKINKIINNIKEVSS

Sequences:

>Translated_360_residues
MSKVNVTKPYLPDINKYKSYVNKIYKNGWLTNNGPLVQELEKRLAKYLGVKNIVLVSNGTIALEIAYRALGVKGSAITTP
FSFVATTSSLVSNNVKPVFVDIDENTLSIDVSKIKYAIEEDTSAIVPVHVFGNGCEVEKIDMLAKKHNLKVIYDAAHAFD
VKYKGESILNYGDISTLSFHATKIFHSIEGGALIINDDSLVEKVRYFINFGIESSESIPYLGTNAKMNEFEAAMGLCVLD
DIIEIKSKRKVITEIYEAGLDGLVKFQEQNQHSSRNYSYFPVIFRTEEELLRVQKALIQNDIISRRYFYPSLDSLSYIEP
KQYMPISRDISKRILCLPIYAELEDDKINKIINNIKEVSS
>Mature_359_residues
SKVNVTKPYLPDINKYKSYVNKIYKNGWLTNNGPLVQELEKRLAKYLGVKNIVLVSNGTIALEIAYRALGVKGSAITTPF
SFVATTSSLVSNNVKPVFVDIDENTLSIDVSKIKYAIEEDTSAIVPVHVFGNGCEVEKIDMLAKKHNLKVIYDAAHAFDV
KYKGESILNYGDISTLSFHATKIFHSIEGGALIINDDSLVEKVRYFINFGIESSESIPYLGTNAKMNEFEAAMGLCVLDD
IIEIKSKRKVITEIYEAGLDGLVKFQEQNQHSSRNYSYFPVIFRTEEELLRVQKALIQNDIISRRYFYPSLDSLSYIEPK
QYMPISRDISKRILCLPIYAELEDDKINKIINNIKEVSS

Specific function: Involved In Eca Elongation. [C]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=266, Percent_Identity=31.203007518797, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI145693159, Length=364, Percent_Identity=25, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 40684; Mature: 40553

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVNVTKPYLPDINKYKSYVNKIYKNGWLTNNGPLVQELEKRLAKYLGVKNIVLVSNGT
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCE
IALEIAYRALGVKGSAITTPFSFVATTSSLVSNNVKPVFVDIDENTLSIDVSKIKYAIEE
EEEEEEEHHHCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHEEEEEC
DTSAIVPVHVFGNGCEVEKIDMLAKKHNLKVIYDAAHAFDVKYKGESILNYGDISTLSFH
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCEEEEEEECCCCEECCCCCCEEEHH
ATKIFHSIEGGALIINDDSLVEKVRYFINFGIESSESIPYLGTNAKMNEFEAAMGLCVLD
HHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHH
DIIEIKSKRKVITEIYEAGLDGLVKFQEQNQHSSRNYSYFPVIFRTEEELLRVQKALIQN
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHHHHHHHH
DIISRRYFYPSLDSLSYIEPKQYMPISRDISKRILCLPIYAELEDDKINKIINNIKEVSS
HHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVNVTKPYLPDINKYKSYVNKIYKNGWLTNNGPLVQELEKRLAKYLGVKNIVLVSNGT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCE
IALEIAYRALGVKGSAITTPFSFVATTSSLVSNNVKPVFVDIDENTLSIDVSKIKYAIEE
EEEEEEEHHHCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHHHEEEEEC
DTSAIVPVHVFGNGCEVEKIDMLAKKHNLKVIYDAAHAFDVKYKGESILNYGDISTLSFH
CCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCEEEEEEECCCCEECCCCCCEEEHH
ATKIFHSIEGGALIINDDSLVEKVRYFINFGIESSESIPYLGTNAKMNEFEAAMGLCVLD
HHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHH
DIIEIKSKRKVITEIYEAGLDGLVKFQEQNQHSSRNYSYFPVIFRTEEELLRVQKALIQN
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEEEEECHHHHHHHHHHHHHH
DIISRRYFYPSLDSLSYIEPKQYMPISRDISKRILCLPIYAELEDDKINKIINNIKEVSS
HHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]