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The map label for this gene is mlaE [H]

Identifier: 134301425

GI number: 134301425

Start: 325468

End: 326232

Strand: Reverse

Name: mlaE [H]

Synonym: FTW_0325

Alternate gene names: 134301425

Gene position: 326232-325468 (Counterclockwise)

Preceding gene: 134301426

Following gene: 134301424

Centisome position: 17.18

GC content: 33.99

Gene sequence:

>765_bases
GTGACAATATTCAGCAAAGTATATACTCTTACATTCTCTTTGATATATAGCTTAGTAAAATCTCTTAGCTTAATACTTAG
AATAGTTATTAGTAAATTGAATATTAGAGATTGTATTATTCAAATAAAAGCTGTCGGAGTTAACTCAATTATTATCATAG
TTACATCTGGTGTTTTCATAGGCTTAGTTTTAAGCTTACAGGGCTATTATACCCTATCAAAATTTGGTGCTCATTCATTG
CTAGGTGTAATGGTGGCTCTTAGTGTACTTAGAGAACTTGGGCCAGTAGTTACAGCGATGCTTTTTGCTGGGCGAGCATG
TAGCTCAATAACTTCAGAAATTGGACTAATGAAAGCCACAGATCAAATCAATAGCCTCAAAGTTATGAATGTTAATCCTA
TAAGCTTCATATTATCTACAAGGTTTTGGGCATGTATGATAAGTGGTCCTATTTTAGCTCTTATCTTTGCAAGCGTTGCA
ATACTTGCAGGTTTCATACTTGCAGAGGCAGCTTTAGGAATTAGTTACGGAGAATTTTGGAGTAATATCCAGTCATCAGT
TATAGCTTCTGATATATCTAATGGAATTATCAAGAGCATTGTATTTGCTTTTATAACTGCGTGGATAGCTTTATACCAAG
GTTATTATTGTATACCAGACTCAAATGGCATAGCCAAAGCTACTACAAAAACAGTTGTTTATTGCTGTATGAGTGTTTTA
GGTGCAGATCTTATTTTAACATCGATTATGTTTGGAGGAGTTTGA

Upstream 100 bases:

>100_bases
ACAAAACTTTCTAGCTGGTAAACCATTAGATTATAATTATCATAATCATAATGATTTAGAGAAAAATTTCTTTAAAAAAG
AGATTCTAGGGAGCTAAGTT

Downstream 100 bases:

>100_bases
TGAGGAACAAATATTTTGAGACCTCAGTAGGAATTTTTATAATAATAGGTGTGCTATGCTTGTTATTTTTAACATTTAAA
GTCAGTGGCACATCATTTAA

Product: ABC-type transporter involved in resistance to organic solvents, permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTIFSKVYTLTFSLIYSLVKSLSLILRIVISKLNIRDCIIQIKAVGVNSIIIIVTSGVFIGLVLSLQGYYTLSKFGAHSL
LGVMVALSVLRELGPVVTAMLFAGRACSSITSEIGLMKATDQINSLKVMNVNPISFILSTRFWACMISGPILALIFASVA
ILAGFILAEAALGISYGEFWSNIQSSVIASDISNGIIKSIVFAFITAWIALYQGYYCIPDSNGIAKATTKTVVYCCMSVL
GADLILTSIMFGGV

Sequences:

>Translated_254_residues
MTIFSKVYTLTFSLIYSLVKSLSLILRIVISKLNIRDCIIQIKAVGVNSIIIIVTSGVFIGLVLSLQGYYTLSKFGAHSL
LGVMVALSVLRELGPVVTAMLFAGRACSSITSEIGLMKATDQINSLKVMNVNPISFILSTRFWACMISGPILALIFASVA
ILAGFILAEAALGISYGEFWSNIQSSVIASDISNGIIKSIVFAFITAWIALYQGYYCIPDSNGIAKATTKTVVYCCMSVL
GADLILTSIMFGGV
>Mature_253_residues
TIFSKVYTLTFSLIYSLVKSLSLILRIVISKLNIRDCIIQIKAVGVNSIIIIVTSGVFIGLVLSLQGYYTLSKFGAHSLL
GVMVALSVLRELGPVVTAMLFAGRACSSITSEIGLMKATDQINSLKVMNVNPISFILSTRFWACMISGPILALIFASVAI
LAGFILAEAALGISYGEFWSNIQSSVIASDISNGIIKSIVFAFITAWIALYQGYYCIPDSNGIAKATTKTVVYCCMSVLG
ADLILTSIMFGGV

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=213, Percent_Identity=52.5821596244132, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27186; Mature: 27055

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIFSKVYTLTFSLIYSLVKSLSLILRIVISKLNIRDCIIQIKAVGVNSIIIIVTSGVFI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEHHHHHH
GLVLSLQGYYTLSKFGAHSLLGVMVALSVLRELGPVVTAMLFAGRACSSITSEIGLMKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQINSLKVMNVNPISFILSTRFWACMISGPILALIFASVAILAGFILAEAALGISYGEFW
HCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SNIQSSVIASDISNGIIKSIVFAFITAWIALYQGYYCIPDSNGIAKATTKTVVYCCMSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHH
GADLILTSIMFGGV
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TIFSKVYTLTFSLIYSLVKSLSLILRIVISKLNIRDCIIQIKAVGVNSIIIIVTSGVFI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEHHHHHH
GLVLSLQGYYTLSKFGAHSLLGVMVALSVLRELGPVVTAMLFAGRACSSITSEIGLMKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQINSLKVMNVNPISFILSTRFWACMISGPILALIFASVAILAGFILAEAALGISYGEFW
HCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SNIQSSVIASDISNGIIKSIVFAFITAWIALYQGYYCIPDSNGIAKATTKTVVYCCMSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHH
GADLILTSIMFGGV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]