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The map label for this gene is putP [H]

Identifier: 134301394

GI number: 134301394

Start: 289067

End: 290533

Strand: Direct

Name: putP [H]

Synonym: FTW_0284

Alternate gene names: 134301394

Gene position: 289067-290533 (Clockwise)

Preceding gene: 134301393

Following gene: 134301395

Centisome position: 15.23

GC content: 35.31

Gene sequence:

>1467_bases
ATGAGTCAAAATAGCATACTTTGGATTACATTTATAATATACATAATAATCATTTTTGGCATAGGGATATACTCATATTT
TCAAACTAAAAAAGTTTCAGATTATATGTTAGGTGGACGTTCCTTAAATGCACCAATTGCAGCACTAGGTGCTGGAGCCT
CTGATATGGGATCATGGTTATTATTAGCTCTTCCTGGAGCGTTTATGGTCTCAGGTATTAACCAAATATGGCTGCCATTG
GGGTTGACAATTGGGGCATTTATAAACTGGGGTGTTATCGCAAGAAGATTGCGAATATATACTGAAATAGCAAGAGATTC
TATTACTATACCTGCCTATTTTGAAAATAGGTTCCACGATAGCAAAGGGATGCTAAGATCGTTAACAGCCATAGTTGTAG
TAATATTTTTTACGATATATATTGGTGCTGGTTTTGTCTCTGGTGGAGTATTATTTAGTTCAATGTTTGGTATTTCATAT
CATCAAGCACTATTACTTACAGCAGCAATAATATTCATTTATACATGCGTTGGTGGTTTTTTGGCAATATCTTGGATTGA
TTTTTTTCAAGGTAGTTTGATGCTGTTAGCTTTGGTTATTGTTCCAATTGTAGTGTATTTTGATATTGGCAGTGACAATA
TTGGTTCGGTGCTATCAAATATTGATATCAAAGGATTTTACGATATTACATCAGGTGTGCCATTAATTACGATTATCTCT
CTTTTGGCATGGGGGTTAGGGTACTTTGGTCAACCACATATTATCGTACGCTTCATGGCAATAAAAGATCCAAATAAGAC
TTCAAAAGCAATGTTTATTTGTATGACGTGGATGATTTTAGCCTTACTAGGAGCTGCTTGTGTTGGTATTTTAGGTGCCG
CATATTATAAGGATGGAATCGCAAATCCAGAATCTGTATTTTTGAAGTTATCAGCAGTATTTTTTAATCCATGGATGGAG
GGCGTTTTATTAGCAGCAGTATTATCTGCAGTGATGAGTACTTCATCGGCGCAACTATTGTCACTATCTAGTGCTTTTTC
GGTTGATGTGTATGCTAAATTCATTCGTAGCAAAGCAAGTCACAGAGAGCTGCTCAATGTCAGTAGATTGATAGTGTTTT
TAGTAACAATTGCGGCAATTATCTTGTCATATAACCCAGATACTACGATTTTAAATTTGGTTGGCTTTTCATGGGCTGGT
CTTGGTAGTTCGTTTGGAGCTGTTGTGATTTTTTCTCTATTTTGGTCAAGAATGAATAAAATTGGAGCTATAGCAGGTAT
ATTATCAGGAGCGCTAGCTGTTTTAATTTGGTCTATGTTTGAGCATTTAGGTGGCTGGTTTAAGGTATATGCGATGGTGC
CTGGTTTTGCACTAAGTAGTATATGTATAATTGTATTTAGCTTGTTAACTTCAAAACCAGAAGAGACAGTACAGTTGGAA
TATCAAAAATATAAACAATCATTATAA

Upstream 100 bases:

>100_bases
AAAATCTTCAAAGAAGTTGTTGTAAATTATCTAAACAAACTTTCAAAAAGTTTCTAAAGATGTTAAATTGGTGTAATTGT
TTTTTTAAAAAAATCTACGG

Downstream 100 bases:

>100_bases
ATTAAATTAATTATTGTTAGTCTAATTTTTTTCAAGTAAAATTCACATCATTTGACTTTAATCATCTTATATGAAAAAAA
CCTTAGTCTTAATATTGGCT

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSQNSILWITFIIYIIIIFGIGIYSYFQTKKVSDYMLGGRSLNAPIAALGAGASDMGSWLLLALPGAFMVSGINQIWLPL
GLTIGAFINWGVIARRLRIYTEIARDSITIPAYFENRFHDSKGMLRSLTAIVVVIFFTIYIGAGFVSGGVLFSSMFGISY
HQALLLTAAIIFIYTCVGGFLAISWIDFFQGSLMLLALVIVPIVVYFDIGSDNIGSVLSNIDIKGFYDITSGVPLITIIS
LLAWGLGYFGQPHIIVRFMAIKDPNKTSKAMFICMTWMILALLGAACVGILGAAYYKDGIANPESVFLKLSAVFFNPWME
GVLLAAVLSAVMSTSSAQLLSLSSAFSVDVYAKFIRSKASHRELLNVSRLIVFLVTIAAIILSYNPDTTILNLVGFSWAG
LGSSFGAVVIFSLFWSRMNKIGAIAGILSGALAVLIWSMFEHLGGWFKVYAMVPGFALSSICIIVFSLLTSKPEETVQLE
YQKYKQSL

Sequences:

>Translated_488_residues
MSQNSILWITFIIYIIIIFGIGIYSYFQTKKVSDYMLGGRSLNAPIAALGAGASDMGSWLLLALPGAFMVSGINQIWLPL
GLTIGAFINWGVIARRLRIYTEIARDSITIPAYFENRFHDSKGMLRSLTAIVVVIFFTIYIGAGFVSGGVLFSSMFGISY
HQALLLTAAIIFIYTCVGGFLAISWIDFFQGSLMLLALVIVPIVVYFDIGSDNIGSVLSNIDIKGFYDITSGVPLITIIS
LLAWGLGYFGQPHIIVRFMAIKDPNKTSKAMFICMTWMILALLGAACVGILGAAYYKDGIANPESVFLKLSAVFFNPWME
GVLLAAVLSAVMSTSSAQLLSLSSAFSVDVYAKFIRSKASHRELLNVSRLIVFLVTIAAIILSYNPDTTILNLVGFSWAG
LGSSFGAVVIFSLFWSRMNKIGAIAGILSGALAVLIWSMFEHLGGWFKVYAMVPGFALSSICIIVFSLLTSKPEETVQLE
YQKYKQSL
>Mature_487_residues
SQNSILWITFIIYIIIIFGIGIYSYFQTKKVSDYMLGGRSLNAPIAALGAGASDMGSWLLLALPGAFMVSGINQIWLPLG
LTIGAFINWGVIARRLRIYTEIARDSITIPAYFENRFHDSKGMLRSLTAIVVVIFFTIYIGAGFVSGGVLFSSMFGISYH
QALLLTAAIIFIYTCVGGFLAISWIDFFQGSLMLLALVIVPIVVYFDIGSDNIGSVLSNIDIKGFYDITSGVPLITIISL
LAWGLGYFGQPHIIVRFMAIKDPNKTSKAMFICMTWMILALLGAACVGILGAAYYKDGIANPESVFLKLSAVFFNPWMEG
VLLAAVLSAVMSTSSAQLLSLSSAFSVDVYAKFIRSKASHRELLNVSRLIVFLVTIAAIILSYNPDTTILNLVGFSWAGL
GSSFGAVVIFSLFWSRMNKIGAIAGILSGALAVLIWSMFEHLGGWFKVYAMVPGFALSSICIIVFSLLTSKPEETVQLEY
QKYKQSL

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=500, Percent_Identity=43.4, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4507031, Length=468, Percent_Identity=23.2905982905983, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4507035, Length=378, Percent_Identity=23.8095238095238, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1787251, Length=492, Percent_Identity=46.3414634146341, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1790503, Length=437, Percent_Identity=26.3157894736842, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87082237, Length=487, Percent_Identity=26.8993839835729, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24645928, Length=384, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI221459584, Length=375, Percent_Identity=24.5333333333333, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53202; Mature: 53071

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCC
>Mature Secondary Structure 
SQNSILWITFIIYIIIIFGIGIYSYFQTKKVSDYMLGGRSLNAPIAALGAGASDMGSWL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHH
LLALPGAFMVSGINQIWLPLGLTIGAFINWGVIARRLRIYTEIARDSITIPAYFENRFHD
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
SKGMLRSLTAIVVVIFFTIYIGAGFVSGGVLFSSMFGISYHQALLLTAAIIFIYTCVGGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAISWIDFFQGSLMLLALVIVPIVVYFDIGSDNIGSVLSNIDIKGFYDITSGVPLITIIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHH
LLAWGLGYFGQPHIIVRFMAIKDPNKTSKAMFICMTWMILALLGAACVGILGAAYYKDGI
HHHHHHCCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ANPESVFLKLSAVFFNPWMEGVLLAAVLSAVMSTSSAQLLSLSSAFSVDVYAKFIRSKAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HRELLNVSRLIVFLVTIAAIILSYNPDTTILNLVGFSWAGLGSSFGAVVIFSLFWSRMNK
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IGAIAGILSGALAVLIWSMFEHLGGWFKVYAMVPGFALSSICIIVFSLLTSKPEETVQLE
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHH
YQKYKQSL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]