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The map label for this gene is hemK [H]

Identifier: 134301376

GI number: 134301376

Start: 269069

End: 269923

Strand: Direct

Name: hemK [H]

Synonym: FTW_0260

Alternate gene names: 134301376

Gene position: 269069-269923 (Clockwise)

Preceding gene: 134301375

Following gene: 134301377

Centisome position: 14.17

GC content: 31.23

Gene sequence:

>855_bases
ATGAGTAATATTACAATTTCTCAATTATTAGCTTTAAGCTTAGCTAAATTTAATCAATGCGATATATCAACCAAACATGA
TCTACAAATGATTATTTGTGATGTTTTGGGTGTTGATAAAACTTATCTTTATATAAATTTGGATAAGCAGTTAGACAAAA
ATACACTTAAAAAAATAGATGAGAAAATCTTACGTCTTTTAGCTGGTGAACCATTGGCTTATATTCTTGGTTATAAGTAT
TTTTGGAATCAAAAACTTTATGTAACTAAAGATACTCTCATACCACGGGCAGATACTGAGACTGTTGTTGCTACAGTGTT
AGATGATATACAAAATAAGGACGCTCAATTAAAAATTTTAGATCTTGGTACAGGTACGGGAGCGATAGCTTTGGCTTTAG
CAGCTGAACTAGCAAATAGTCAAGTTGTTGCGGTTGATTTATATCAGCAGAGTCTTGATGTTGCTAAAAAAAATGCCCAA
GCAAACAATATCACTAACGTTAAATTTATCCAAAGTAGTTGGTATACAAATCTCGATACTGATAAATTTGATATTATAGT
TTCTAATCCACCATATATAGATTTAGCGGATACAAATATTGACCAAAGCGTCAAAGACTATGAACCCGCTCGAGCTTTAT
TTGCTGCAGATAATGGTTTAGCGGATATTAGGATAATTATATCTCAAGCAAAAGATTTTCTAAATCTAGGGGGCTTCATT
TATATAGAGCATGGTTTTACTCAGGCAGATGCTATTACAGCTTTATTTTCTCAGTGTAATTTTACTGATATTAAAATAGT
TAAAGATTTAAATAATAACGATAGATGTACTAAGGCACAACTTGGCTATTTATGA

Upstream 100 bases:

>100_bases
ACTCTTTATAAGCTTGATGAGGTCATGGAAGGTAGTCTAGATAGTATTATCCAACCACTAGTTCTAGAACATCAGGCGGA
TCTTTTAGCAACCATGTCTG

Downstream 100 bases:

>100_bases
TAAGGATGACCCTCTAATATTGTGTAAGCTCTATATAGCTGTTCAGCAATAATAATACGTACTAAAGGATGAGGGAATGT
CATTTTTGAGATAGATATTT

Product: HemK family methyltransferase

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSNITISQLLALSLAKFNQCDISTKHDLQMIICDVLGVDKTYLYINLDKQLDKNTLKKIDEKILRLLAGEPLAYILGYKY
FWNQKLYVTKDTLIPRADTETVVATVLDDIQNKDAQLKILDLGTGTGAIALALAAELANSQVVAVDLYQQSLDVAKKNAQ
ANNITNVKFIQSSWYTNLDTDKFDIIVSNPPYIDLADTNIDQSVKDYEPARALFAADNGLADIRIIISQAKDFLNLGGFI
YIEHGFTQADAITALFSQCNFTDIKIVKDLNNNDRCTKAQLGYL

Sequences:

>Translated_284_residues
MSNITISQLLALSLAKFNQCDISTKHDLQMIICDVLGVDKTYLYINLDKQLDKNTLKKIDEKILRLLAGEPLAYILGYKY
FWNQKLYVTKDTLIPRADTETVVATVLDDIQNKDAQLKILDLGTGTGAIALALAAELANSQVVAVDLYQQSLDVAKKNAQ
ANNITNVKFIQSSWYTNLDTDKFDIIVSNPPYIDLADTNIDQSVKDYEPARALFAADNGLADIRIIISQAKDFLNLGGFI
YIEHGFTQADAITALFSQCNFTDIKIVKDLNNNDRCTKAQLGYL
>Mature_283_residues
SNITISQLLALSLAKFNQCDISTKHDLQMIICDVLGVDKTYLYINLDKQLDKNTLKKIDEKILRLLAGEPLAYILGYKYF
WNQKLYVTKDTLIPRADTETVVATVLDDIQNKDAQLKILDLGTGTGAIALALAAELANSQVVAVDLYQQSLDVAKKNAQA
NNITNVKFIQSSWYTNLDTDKFDIIVSNPPYIDLADTNIDQSVKDYEPARALFAADNGLADIRIIISQAKDFLNLGGFIY
IEHGFTQADAITALFSQCNFTDIKIVKDLNNNDRCTKAQLGYL

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=232, Percent_Identity=30.6034482758621, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787463, Length=259, Percent_Identity=38.6100386100386, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI87082085, Length=250, Percent_Identity=29.2, Blast_Score=104, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324266, Length=154, Percent_Identity=34.4155844155844, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582226, Length=230, Percent_Identity=25.6521739130435, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31643; Mature: 31511

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNITISQLLALSLAKFNQCDISTKHDLQMIICDVLGVDKTYLYINLDKQLDKNTLKKID
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHEEHHHHCCCCEEEEEEECCCCCHHHHHHHH
EKILRLLAGEPLAYILGYKYFWNQKLYVTKDTLIPRADTETVVATVLDDIQNKDAQLKIL
HHHHHHHCCCCHHHHHCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
DLGTGTGAIALALAAELANSQVVAVDLYQQSLDVAKKNAQANNITNVKFIQSSWYTNLDT
EECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
DKFDIIVSNPPYIDLADTNIDQSVKDYEPARALFAADNGLADIRIIISQAKDFLNLGGFI
CCEEEEECCCCEEEEECCCCCCCHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHCCCEE
YIEHGFTQADAITALFSQCNFTDIKIVKDLNNNDRCTKAQLGYL
EEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNITISQLLALSLAKFNQCDISTKHDLQMIICDVLGVDKTYLYINLDKQLDKNTLKKID
CCCHHHHHHHHHHHHCCCCCCCCCCCHHHEEHHHHCCCCEEEEEEECCCCCHHHHHHHH
EKILRLLAGEPLAYILGYKYFWNQKLYVTKDTLIPRADTETVVATVLDDIQNKDAQLKIL
HHHHHHHCCCCHHHHHCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEE
DLGTGTGAIALALAAELANSQVVAVDLYQQSLDVAKKNAQANNITNVKFIQSSWYTNLDT
EECCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCC
DKFDIIVSNPPYIDLADTNIDQSVKDYEPARALFAADNGLADIRIIISQAKDFLNLGGFI
CCEEEEECCCCEEEEECCCCCCCHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHHCCCEE
YIEHGFTQADAITALFSQCNFTDIKIVKDLNNNDRCTKAQLGYL
EEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]