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The map label for this gene is trmD

Identifier: 134301361

GI number: 134301361

Start: 253263

End: 254021

Strand: Direct

Name: trmD

Synonym: FTW_0242

Alternate gene names: 134301361

Gene position: 253263-254021 (Clockwise)

Preceding gene: 134301360

Following gene: 134301362

Centisome position: 13.34

GC content: 35.84

Gene sequence:

>759_bases
ATGAAATTTGGGATAATATCAATATTTCCAGAGATGTTTAAAGCAATAAATGATTTTGGAGTTACAGCAAGAGCTATAAA
GGACTCTAAAGTATCGATAAGTTGCTTTAATCCGCGTGACTACACTACAGATAGACATGCTACAGTCGATGATACTAGTT
TTGGTGGTGGCGCAGGGATGGTAATGAAGTATCAACCTTTATCAGCAGCTATTGAAGATGCAAAAAATACTTTAGGTTGT
GGTACAAAAGTAGTATACTTGTCACCTCAGGGTAGTATATTTAATCATAGAAAAGCCCAAGAGCTTTTGCAAAATGATTC
ATTAATACTGCTCTGTGGTAGATACGAAGGGGTTGATGAGCGCCTAATACAAGATTATGTTGACGAAGAAATTTCGGTTG
GAGATTTTGTGTTAAGTGGTGGTGAGCTCCCTGCTATGCTAGTTATGGATAGTCTAATTAGGCTGTTACCGGAAGTGTTG
GGGAATAAAGAATCTGTCGTAGAAGATTCATTTTATGATGGTCTTTTGGATTATCCACACTATACAAAACCGGCTGTTTT
ACCTAATGGTAATGCAGTACCTGACGTTTTGTTATCTGGTAATCATAAAGAAATAGCTAAATGGAGGCGTAAACAAAAGT
TAATAAGAACTTATGAGCGTCGTAAAGATTTAATAGAGTGCCTATGCCTATCTGCAAAAGATAAGCAAATTTTAGATGAT
TATAAAATAGATAAGGTAAGCACAAAAGGAGAAGAATAA

Upstream 100 bases:

>100_bases
TTCCTTACTATAAAAGTTTATTTTATCGCTCAAATATTTGTGTTTACTTTCGATAAGAACTACAATTAAGTCTGTTTAGC
TAAAGATTAAATTTTTGAAG

Downstream 100 bases:

>100_bases
TGAAAAATAAATTTGTTGAGCTAGTAGAAAAGTCACAATTAAGAACTGATCTTCCAGAATTTAATCCAGGTGATTCTATT
ACTGTTAATTTATGGATTAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKFGIISIFPEMFKAINDFGVTARAIKDSKVSISCFNPRDYTTDRHATVDDTSFGGGAGMVMKYQPLSAAIEDAKNTLGC
GTKVVYLSPQGSIFNHRKAQELLQNDSLILLCGRYEGVDERLIQDYVDEEISVGDFVLSGGELPAMLVMDSLIRLLPEVL
GNKESVVEDSFYDGLLDYPHYTKPAVLPNGNAVPDVLLSGNHKEIAKWRRKQKLIRTYERRKDLIECLCLSAKDKQILDD
YKIDKVSTKGEE

Sequences:

>Translated_252_residues
MKFGIISIFPEMFKAINDFGVTARAIKDSKVSISCFNPRDYTTDRHATVDDTSFGGGAGMVMKYQPLSAAIEDAKNTLGC
GTKVVYLSPQGSIFNHRKAQELLQNDSLILLCGRYEGVDERLIQDYVDEEISVGDFVLSGGELPAMLVMDSLIRLLPEVL
GNKESVVEDSFYDGLLDYPHYTKPAVLPNGNAVPDVLLSGNHKEIAKWRRKQKLIRTYERRKDLIECLCLSAKDKQILDD
YKIDKVSTKGEE
>Mature_252_residues
MKFGIISIFPEMFKAINDFGVTARAIKDSKVSISCFNPRDYTTDRHATVDDTSFGGGAGMVMKYQPLSAAIEDAKNTLGC
GTKVVYLSPQGSIFNHRKAQELLQNDSLILLCGRYEGVDERLIQDYVDEEISVGDFVLSGGELPAMLVMDSLIRLLPEVL
GNKESVVEDSFYDGLLDYPHYTKPAVLPNGNAVPDVLLSGNHKEIAKWRRKQKLIRTYERRKDLIECLCLSAKDKQILDD
YKIDKVSTKGEE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=249, Percent_Identity=55.421686746988, Blast_Score=273, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_FRAT1 (Q14JS7)

Other databases:

- EMBL:   AM286280
- RefSeq:   YP_666349.1
- ProteinModelPortal:   Q14JS7
- SMR:   Q14JS7
- STRING:   Q14JS7
- GeneID:   4199863
- GenomeReviews:   AM286280_GR
- KEGG:   ftf:FTF0152
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q14JS7
- ProtClustDB:   PRK00026
- BioCyc:   FTUL393115:FTF0152-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28164; Mature: 28164

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFGIISIFPEMFKAINDFGVTARAIKDSKVSISCFNPRDYTTDRHATVDDTSFGGGAGM
CCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VMKYQPLSAAIEDAKNTLGCGTKVVYLSPQGSIFNHRKAQELLQNDSLILLCGRYEGVDE
EEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHH
RLIQDYVDEEISVGDFVLSGGELPAMLVMDSLIRLLPEVLGNKESVVEDSFYDGLLDYPH
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
YTKPAVLPNGNAVPDVLLSGNHKEIAKWRRKQKLIRTYERRKDLIECLCLSAKDKQILDD
CCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
YKIDKVSTKGEE
HCCCCCCCCCCC
>Mature Secondary Structure
MKFGIISIFPEMFKAINDFGVTARAIKDSKVSISCFNPRDYTTDRHATVDDTSFGGGAGM
CCCCHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VMKYQPLSAAIEDAKNTLGCGTKVVYLSPQGSIFNHRKAQELLQNDSLILLCGRYEGVDE
EEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHH
RLIQDYVDEEISVGDFVLSGGELPAMLVMDSLIRLLPEVLGNKESVVEDSFYDGLLDYPH
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
YTKPAVLPNGNAVPDVLLSGNHKEIAKWRRKQKLIRTYERRKDLIECLCLSAKDKQILDD
CCCCCCCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
YKIDKVSTKGEE
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA