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The map label for this gene is glpK [H]

Identifier: 134301339

GI number: 134301339

Start: 226417

End: 227925

Strand: Direct

Name: glpK [H]

Synonym: FTW_0219

Alternate gene names: 134301339

Gene position: 226417-227925 (Clockwise)

Preceding gene: 134301338

Following gene: 134301340

Centisome position: 11.93

GC content: 38.3

Gene sequence:

>1509_bases
ATGTCCACAGATTTCATCTTAGCTGTAGATCAAGGTACAACAAGTTCACGAGCAATTATCTTTGATAAAAAGGGTAATAT
AAGAAAGATTGCTCAAAAAGAATTCACACAAATATATCCTAAAAGTGGTTGGGTAGAACATGATGCAATGGAAATATGGG
GTACTCAAAGTGGCGTAATGCGTGAGGCACTAGAGTTTGGCCGCGTTAAGCCAGATCAAATTGCAGCAATAGGTATTACA
AATCAGCGCGAGACAGTTGTAGTTTGGGATAAAGAAACAGGTGATCCTGTCTATAATGCTATAGTGTGGCAATGTCGCCG
TACTTCGAGTATTTGTGATGAAATCAAACGAGATCCTCAATTTGTCAAATATATCAAAGAAAATACTGGTCTGGTTGTAG
ATGCGTATTTTTCAGGCACTAAAGTAAAATGGATTTTAGATAACGTTGAAGGTGCTAGAGAAAAAGCAAATGCCGGCAAA
TTATTAATGGGTACTATAGATACTTGGTTGATTTGGAATCTAACTCGCGGTAAAGTGCATGCGACAGACTATAGTAATGC
TTCGCGCACTATGTTATTTAATATTAATAGTTTAGAGTGGGATAAAAAAATACTTGATTACTTAAATATTCCTGAATCAA
TGTTACCTGAGGTTAAAAACTCAAGTGAGGTTTTTGGAGTTACAGATAGCCATACTTTAGGTGGTGCTGAGATTCCTATT
GCTGGTGTTGCAGGAGATCAGCATGCAGCATTATTTGGTCATTGCTGTTTTGAAAAAGGTATGGCAAAAAATACTTATGG
TACGGGTTGTTTTGCGTTGATGAATGTCGGCGATAAGCCAGTTTATTCAGATGAAGGTTTATTGACCACTATCGCATGGG
CAGAAAATGGTAAGCCAACTTATGCTTTAGAAGGTAGTGTATTTATAGCAGGAGCTGTTATTCAGTGGATTCGTGATGGT
TTGGGACTAGTGCGTTCTGCTGAGGATAGTGAGTACTATGCTACTAAGATTGATTCTACAAATGGTGTATATTTGGTGCC
TGCGTTTGTTGGTCTTGGCACACCATATTGGGATATGTATGCTCGTGGTACGATAGTTGGCATTACCCGCGATACAAAGC
GTGAGCATATTATTCGTGCAGCTCTAGAGGCTATAGCATATCAAGCTAAAGATGTCTTAGAATGTATGAAAGAGGATACG
GGACTTGATCTTGCTGGTTTGCGTGTTGATGGTGGCGCGGTACAAAATAACTTCCTAATGCAGTTTCAATCTGATATTTT
ACAATCTGAAATCTCAAAACCGAAAATAAATGAAATTACTGGTTTGGGGGCTGTCTTCTTAGCTGGTTTAGCAGTTGGTT
TTTGGAAGGATAAACAAGAATTGAAATCTATTCTTACAACTGAAAAAGTTTTTGAACCGCAAAAAGACTCTCAAGCTGTT
GCTCATGATTATAGAGGTTGGAAGAAAGCAGTAGAAAGAAGTAAGGCTTGGGCAGAGTGTTATAGTTAA

Upstream 100 bases:

>100_bases
TATCAATAGTATTATCTCTTTTTATTACGGAGAAAAAAAACTAAAATATCCTTTAACAATATACAATTTGTATTAATCTT
TACAAAAAGGAATATCTATT

Downstream 100 bases:

>100_bases
TCCGAGAGTATTTTGCTAAACACATAAGAGATAGCATCACAAAATTTTTCAAAACTATTATTCACTTTTCTAAATATTTT
TTTAAAGTTAGCCCAAACCT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSTDFILAVDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVMREALEFGRVKPDQIAAIGIT
NQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQFVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGK
LLMGTIDTWLIWNLTRGKVHATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPTYALEGSVFIAGAVIQWIRDG
LGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMYARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDT
GLDLAGLRVDGGAVQNNFLMQFQSDILQSEISKPKINEITGLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
AHDYRGWKKAVERSKAWAECYS

Sequences:

>Translated_502_residues
MSTDFILAVDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVMREALEFGRVKPDQIAAIGIT
NQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQFVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGK
LLMGTIDTWLIWNLTRGKVHATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPTYALEGSVFIAGAVIQWIRDG
LGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMYARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDT
GLDLAGLRVDGGAVQNNFLMQFQSDILQSEISKPKINEITGLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
AHDYRGWKKAVERSKAWAECYS
>Mature_501_residues
STDFILAVDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVMREALEFGRVKPDQIAAIGITN
QRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQFVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGKL
LMGTIDTWLIWNLTRGKVHATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPIA
GVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPTYALEGSVFIAGAVIQWIRDGL
GLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMYARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDTG
LDLAGLRVDGGAVQNNFLMQFQSDILQSEISKPKINEITGLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAVA
HDYRGWKKAVERSKAWAECYS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=47.0119521912351, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=47.0119521912351, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=47.0355731225296, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=45.8167330677291, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=34.0551181102362, Blast_Score=322, Evalue=5e-88,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=62.2222222222222, Blast_Score=665, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=472, Percent_Identity=24.1525423728814, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787789, Length=517, Percent_Identity=23.4042553191489, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=40.990099009901, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=25.5769230769231, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71986147, Length=297, Percent_Identity=28.6195286195286, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=558, Percent_Identity=33.3333333333333, Blast_Score=286, Evalue=6e-78,
Organism=Drosophila melanogaster, GI17864214, Length=514, Percent_Identity=42.6070038910506, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655555, Length=510, Percent_Identity=37.4509803921569, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=510, Percent_Identity=37.4509803921569, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=510, Percent_Identity=37.4509803921569, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=510, Percent_Identity=37.4509803921569, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=510, Percent_Identity=37.4509803921569, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24654573, Length=440, Percent_Identity=43.8636363636364, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=31.8785578747628, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=31.8458417849899, Blast_Score=262, Evalue=5e-70,
Organism=Drosophila melanogaster, GI24656367, Length=520, Percent_Identity=29.2307692307692, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=28.5444234404537, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24656372, Length=350, Percent_Identity=31.1428571428571, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=29.6551724137931, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55705; Mature: 55574

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDFILAVDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVM
CCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHH
REALEFGRVKPDQIAAIGITNQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQ
HHHHHHCCCCCHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGKLLMGTIDTWLIWNLTRGKVH
HHHHHHHCCCEEEEEEECCCEEEEEEECCCHHHHHCCCCCEEEEECCEEEEEEECCCEEE
ATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
ECCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEECCCCCCCCCCCCEE
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPT
EECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCE
YALEGSVFIAGAVIQWIRDGLGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMY
EEECCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHHCCCCHHHHH
ARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDTGLDLAGLRVDGGAVQNNFLM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
QFQSDILQSEISKPKINEITGLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHH
AHDYRGWKKAVERSKAWAECYS
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STDFILAVDQGTTSSRAIIFDKKGNIRKIAQKEFTQIYPKSGWVEHDAMEIWGTQSGVM
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHH
REALEFGRVKPDQIAAIGITNQRETVVVWDKETGDPVYNAIVWQCRRTSSICDEIKRDPQ
HHHHHHCCCCCHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
FVKYIKENTGLVVDAYFSGTKVKWILDNVEGAREKANAGKLLMGTIDTWLIWNLTRGKVH
HHHHHHHCCCEEEEEEECCCEEEEEEECCCHHHHHCCCCCEEEEECCEEEEEEECCCEEE
ATDYSNASRTMLFNINSLEWDKKILDYLNIPESMLPEVKNSSEVFGVTDSHTLGGAEIPI
ECCCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEECCCCCCCCCCCCEE
AGVAGDQHAALFGHCCFEKGMAKNTYGTGCFALMNVGDKPVYSDEGLLTTIAWAENGKPT
EECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCE
YALEGSVFIAGAVIQWIRDGLGLVRSAEDSEYYATKIDSTNGVYLVPAFVGLGTPYWDMY
EEECCCEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHHCCCCHHHHH
ARGTIVGITRDTKREHIIRAALEAIAYQAKDVLECMKEDTGLDLAGLRVDGGAVQNNFLM
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHH
QFQSDILQSEISKPKINEITGLGAVFLAGLAVGFWKDKQELKSILTTEKVFEPQKDSQAV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHH
AHDYRGWKKAVERSKAWAECYS
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA