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The map label for this gene is sigE [H]

Identifier: 134300490

GI number: 134300490

Start: 2875743

End: 2876441

Strand: Reverse

Name: sigE [H]

Synonym: Dred_2655

Alternate gene names: 134300490

Gene position: 2876441-2875743 (Counterclockwise)

Preceding gene: 134300496

Following gene: 134300486

Centisome position: 79.72

GC content: 41.2

Gene sequence:

>699_bases
TTGATCACTGGCATTGTTACGTTACTTGCCCTTACTATTCTCAATGGTATAGCAATTCTGGTTTCTTATATTTCTTCCGG
CAGTTTACCGGATCCTTTATCCCCGGAAGAAGAAAGGGAGTTATTATTAAAAATGGCAACCGGAGATAAGTTAGCTAAAA
CGATTCTGATCGAAAGGAATTTGCGATTGGTTGCTAAGATTGCCAATAAGTTGAAAGATAGCGGTGTCGATAAGCAAGAT
TTGTTTCAAGTGGGGGTCATTGGTTTAATTAAAGGTGTAGATACCTTTGATATAAAGAAGGCGAGAAAGTTTTCCACCTA
TGCTGGTGTTTGCATTCAAAATGAAATGCTAATGATGTTGCGCAAGCAGAAAAGTGAGCGAAATACCATCTCTATTTCTG
AACCTCTGGATTTTGATAAAGACGGCAGTCCTTTGGAGTTGCAGGATACCCTGGCCAATAACGAAATCCCGGTGGATGAA
CTGGTGGAGAAAAGCTTGGATGTGGAGAGGTTGGAAAAGGCCCTTCAAACACTTAATCCCAGAGAGAAAATCGTCATTAA
ATTACGATATGGTCTGGAGGGTGAAAGAGAGTTGACCCAACGGGAGGTGGGTAAGAGGCTAGATATATCCCGCTCCTTTA
TCTCCCGCATTGAGAGCAGTACCATCAATAAACTTTGTAGAGAAATGTCGCATTCATAA

Upstream 100 bases:

>100_bases
AGAACTTTCTATCAAATTTTTTTGTGTTGAACATGCACTTTTCCTAAGGCTGCTTCGTAGCATAGAGTAGATAACTATTT
TTGTTACGGAGGGATGATCA

Downstream 100 bases:

>100_bases
GCGGTGATAACGTGAATAGCTAAATATTTGTACAAAGTAAAGGCCCCCGAAACTCGGGAGCCTTTACACCTTTATTGTAT
TAGAAGGTAGCAACAACAGC

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: P31; Sigma-29; Stage II sporulation protein GB [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MITGIVTLLALTILNGIAILVSYISSGSLPDPLSPEEERELLLKMATGDKLAKTILIERNLRLVAKIANKLKDSGVDKQD
LFQVGVIGLIKGVDTFDIKKARKFSTYAGVCIQNEMLMMLRKQKSERNTISISEPLDFDKDGSPLELQDTLANNEIPVDE
LVEKSLDVERLEKALQTLNPREKIVIKLRYGLEGERELTQREVGKRLDISRSFISRIESSTINKLCREMSHS

Sequences:

>Translated_232_residues
MITGIVTLLALTILNGIAILVSYISSGSLPDPLSPEEERELLLKMATGDKLAKTILIERNLRLVAKIANKLKDSGVDKQD
LFQVGVIGLIKGVDTFDIKKARKFSTYAGVCIQNEMLMMLRKQKSERNTISISEPLDFDKDGSPLELQDTLANNEIPVDE
LVEKSLDVERLEKALQTLNPREKIVIKLRYGLEGERELTQREVGKRLDISRSFISRIESSTINKLCREMSHS
>Mature_232_residues
MITGIVTLLALTILNGIAILVSYISSGSLPDPLSPEEERELLLKMATGDKLAKTILIERNLRLVAKIANKLKDSGVDKQD
LFQVGVIGLIKGVDTFDIKKARKFSTYAGVCIQNEMLMMLRKQKSERNTISISEPLDFDKDGSPLELQDTLANNEIPVDE
LVEKSLDVERLEKALQTLNPREKIVIKLRYGLEGERELTQREVGKRLDISRSFISRIESSTINKLCREMSHS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=250, Percent_Identity=27.2, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26040; Mature: 26040

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGIVTLLALTILNGIAILVSYISSGSLPDPLSPEEERELLLKMATGDKLAKTILIERN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
LRLVAKIANKLKDSGVDKQDLFQVGVIGLIKGVDTFDIKKARKFSTYAGVCIQNEMLMML
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQKSERNTISISEPLDFDKDGSPLELQDTLANNEIPVDELVEKSLDVERLEKALQTLNP
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCC
REKIVIKLRYGLEGERELTQREVGKRLDISRSFISRIESSTINKLCREMSHS
CHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITGIVTLLALTILNGIAILVSYISSGSLPDPLSPEEERELLLKMATGDKLAKTILIERN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCC
LRLVAKIANKLKDSGVDKQDLFQVGVIGLIKGVDTFDIKKARKFSTYAGVCIQNEMLMML
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQKSERNTISISEPLDFDKDGSPLELQDTLANNEIPVDELVEKSLDVERLEKALQTLNP
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCC
REKIVIKLRYGLEGERELTQREVGKRLDISRSFISRIESSTINKLCREMSHS
CHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6438529; 9384377; 3104904; 2106671 [H]