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The map label for this gene is yqiI [H]

Identifier: 134300415

GI number: 134300415

Start: 2805265

End: 2805987

Strand: Reverse

Name: yqiI [H]

Synonym: Dred_2576

Alternate gene names: 134300415

Gene position: 2805987-2805265 (Counterclockwise)

Preceding gene: 134300416

Following gene: 134300413

Centisome position: 77.77

GC content: 44.4

Gene sequence:

>723_bases
ATGTACATCAAAGGCCCTTACAGAAAAAAAAGAAATCCATGGTTCTTACCTATCTTAGTTGTGGTTATTTTGCTTCCCGC
AGGCGTTTTTTTAAAAGGATGTCTTTATAGATCCGAAGCAAAACCGCCTCCCAAACCCCAAAAAAAACCCATCTCAAACC
ATGTTCTTATCATTGACCCCGGGCACGGTGGAACAGATCCCGGGGCCTGCCGGGAAGGAATAATGGAAAAGGATATCAAT
TTGGCCATTGCCAAAAGGGTAGCAAAACATATTGAGGGCCATAGGGTACGACTGACCCGAGAAAAGGACGTTGATTTCGT
ACAAAAGGGTGTGTACACGAGGGATGCAGAACGGCAGGACTTGGAGAGTAGAATTGACTTAGCTCAAAAATATCATGGAG
AAGTTTTTGTAAGCATCCATATCAACACTGGTGAGGGCCAAGACAGTGGGGTTTTGGTTTATTATGATCCCAAGGACCCG
GCAAGTACAAGGTTGGCACAGGCCATTCAACTAGAGGCTAACAATTTACCTGATTCGCCAGATAAAAAGCCCCGCCCTGA
TGATTTTTACTTGTTTAATAATTTGAAAATACCAGTGGTCATTGTGGAAGCCGGTTGGCTTTGTAATCCAACGGAAAGAA
AACGGCTGATGGACCCGGTCTACCAGGAACAAGTGGCCAATGCCATCGCCCGAGGAATAACAAATTTTTTAGACACTCTG
TAA

Upstream 100 bases:

>100_bases
GAAACAAATACTGCCACCAGCTCTTAAAAAATCCCATTCTCAAAAGAGTATGAAGCAAGGCAGGTTTCCTAAAATAAAAC
AAGTGAAGGTAAAAAAAAGC

Downstream 100 bases:

>100_bases
TGAAAAGTAAAAAAGTTTTAACGCTATCTATGCTTTGAGCGAGCGGAGATAATGAAATTCCTGCTAGAGAGGTTGATCAA
AAAATTCATATAAAAAAAGC

Product: cell wall hydrolase/autolysin

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MYIKGPYRKKRNPWFLPILVVVILLPAGVFLKGCLYRSEAKPPPKPQKKPISNHVLIIDPGHGGTDPGACREGIMEKDIN
LAIAKRVAKHIEGHRVRLTREKDVDFVQKGVYTRDAERQDLESRIDLAQKYHGEVFVSIHINTGEGQDSGVLVYYDPKDP
ASTRLAQAIQLEANNLPDSPDKKPRPDDFYLFNNLKIPVVIVEAGWLCNPTERKRLMDPVYQEQVANAIARGITNFLDTL

Sequences:

>Translated_240_residues
MYIKGPYRKKRNPWFLPILVVVILLPAGVFLKGCLYRSEAKPPPKPQKKPISNHVLIIDPGHGGTDPGACREGIMEKDIN
LAIAKRVAKHIEGHRVRLTREKDVDFVQKGVYTRDAERQDLESRIDLAQKYHGEVFVSIHINTGEGQDSGVLVYYDPKDP
ASTRLAQAIQLEANNLPDSPDKKPRPDDFYLFNNLKIPVVIVEAGWLCNPTERKRLMDPVYQEQVANAIARGITNFLDTL
>Mature_240_residues
MYIKGPYRKKRNPWFLPILVVVILLPAGVFLKGCLYRSEAKPPPKPQKKPISNHVLIIDPGHGGTDPGACREGIMEKDIN
LAIAKRVAKHIEGHRVRLTREKDVDFVQKGVYTRDAERQDLESRIDLAQKYHGEVFVSIHINTGEGQDSGVLVYYDPKDP
ASTRLAQAIQLEANNLPDSPDKKPRPDDFYLFNNLKIPVVIVEAGWLCNPTERKRLMDPVYQEQVANAIARGITNFLDTL

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=253, Percent_Identity=25.296442687747, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 27101; Mature: 27101

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIKGPYRKKRNPWFLPILVVVILLPAGVFLKGCLYRSEAKPPPKPQKKPISNHVLIIDP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
GHGGTDPGACREGIMEKDINLAIAKRVAKHIEGHRVRLTREKDVDFVQKGVYTRDAERQD
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCHHHH
LESRIDLAQKYHGEVFVSIHINTGEGQDSGVLVYYDPKDPASTRLAQAIQLEANNLPDSP
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
DKKPRPDDFYLFNNLKIPVVIVEAGWLCNPTERKRLMDPVYQEQVANAIARGITNFLDTL
CCCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYIKGPYRKKRNPWFLPILVVVILLPAGVFLKGCLYRSEAKPPPKPQKKPISNHVLIIDP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
GHGGTDPGACREGIMEKDINLAIAKRVAKHIEGHRVRLTREKDVDFVQKGVYTRDAERQD
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCHHHH
LESRIDLAQKYHGEVFVSIHINTGEGQDSGVLVYYDPKDPASTRLAQAIQLEANNLPDSP
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
DKKPRPDDFYLFNNLKIPVVIVEAGWLCNPTERKRLMDPVYQEQVANAIARGITNFLDTL
CCCCCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]