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The map label for this gene is comEC [H]

Identifier: 134300413

GI number: 134300413

Start: 2803522

End: 2804580

Strand: Reverse

Name: comEC [H]

Synonym: Dred_2574

Alternate gene names: 134300413

Gene position: 2804580-2803522 (Counterclockwise)

Preceding gene: 134300415

Following gene: 134300412

Centisome position: 77.73

GC content: 45.33

Gene sequence:

>1059_bases
ATGAAGAAGTTTATTATCACTTTACTTACTATTCTACTGGTGACAACGGGTTGCAGTCAGTCGAAGGAGAAACCTTCTTC
CACACCCTCTGTACCCTCCGGTAGTCTACGGGTTCATTTTATTGATGTGGGGCAGGCGGATGCTATCCTGGTGCAAAGGG
GAGAGGATTCCATGCTGGTGGATGCCGGAAATAATGATGACGGGGAAAAAATAATACGTTACTTAAGTCAGCAGGGAATC
AAAAAGTTATCCGTGGTCATGGGTACGCACCCCCACGAGGATCATATTGGTGGCTTGGACGATGTAATCAAGTATTTCCC
GGTGGAGCGGGTGTATTTACCAAAGGTGAATCATACAACCAAAACCTATCGGGATGTGCTGACGGCTATCAAGAACAAAA
ACCTTAAGGCTACTGCTGCCTTTGGGGGACAGACTTTTAACCTCGAGGATGCTCGGGTGGAGATTTTGGCTCCCAACAGC
AGTGAGTATAAAGAACTGAATGATTACAGTATTGTTAGTAAAATTTCTTTTGGAGATACGGCTTTCTTATTGACCGGTGA
TGCAGAGATGTTATCAGAACAAGAAATGTTAGAGAAGGGTTTTGATCTAGAAGCCAATGTTCTCAAAGTGGGCCACCATG
GCAGCCATTCCTCTACCGGAGATAAATTTCTTAGGGCAGTCAATCCCCAGATGGCAGTGATCTCGGTGGGTGCCAACAAC
GATTATGGTCACCCCCACCGGGAAACTCTGCAGCGGTTGAATGATAGCAAGATCAAAGTTTACCAAACGTCCCAACAGGG
TACAATTGTAATGACCTCTGATGGGAAGCGGGTTGAGGTGCAAGGAGAAATAACCGAAGCTGAAAAAGCTGTACCAAAGG
AATCCACAGCAAATGTTGGAAATGATGGTAAGGGTTTAATTAAAGGAAACATTAATAAAAACGGGGATAAAATTTATCAT
CTGCCGGGACAGCAGAACTATGATCGAACCAAACCGGAAGTATGGTTTAAAACGGAGCAAGAGGCACAGGCGGCAGGTTT
TAGGAGGGCAAGCCGATGA

Upstream 100 bases:

>100_bases
AATCTCTTATAAAGTAACTTAAACTGGTTGAACCAAAATCTGGTTAATCAATATGATATAATGATCTACTGTATTGATCA
TAAACCAAGGGAGAGAGAAT

Downstream 100 bases:

>100_bases
AGGCAGTGGTCGATCGTTTTGAAGGTGCATGGGCAGTTTTAGAAGTGCAGGGTAAAATGATGTGGAATGTTCCTAGGGAT
TTTTTGCCCTCCGGGACCAG

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKKFIITLLTILLVTTGCSQSKEKPSSTPSVPSGSLRVHFIDVGQADAILVQRGEDSMLVDAGNNDDGEKIIRYLSQQGI
KKLSVVMGTHPHEDHIGGLDDVIKYFPVERVYLPKVNHTTKTYRDVLTAIKNKNLKATAAFGGQTFNLEDARVEILAPNS
SEYKELNDYSIVSKISFGDTAFLLTGDAEMLSEQEMLEKGFDLEANVLKVGHHGSHSSTGDKFLRAVNPQMAVISVGANN
DYGHPHRETLQRLNDSKIKVYQTSQQGTIVMTSDGKRVEVQGEITEAEKAVPKESTANVGNDGKGLIKGNINKNGDKIYH
LPGQQNYDRTKPEVWFKTEQEAQAAGFRRASR

Sequences:

>Translated_352_residues
MKKFIITLLTILLVTTGCSQSKEKPSSTPSVPSGSLRVHFIDVGQADAILVQRGEDSMLVDAGNNDDGEKIIRYLSQQGI
KKLSVVMGTHPHEDHIGGLDDVIKYFPVERVYLPKVNHTTKTYRDVLTAIKNKNLKATAAFGGQTFNLEDARVEILAPNS
SEYKELNDYSIVSKISFGDTAFLLTGDAEMLSEQEMLEKGFDLEANVLKVGHHGSHSSTGDKFLRAVNPQMAVISVGANN
DYGHPHRETLQRLNDSKIKVYQTSQQGTIVMTSDGKRVEVQGEITEAEKAVPKESTANVGNDGKGLIKGNINKNGDKIYH
LPGQQNYDRTKPEVWFKTEQEAQAAGFRRASR
>Mature_352_residues
MKKFIITLLTILLVTTGCSQSKEKPSSTPSVPSGSLRVHFIDVGQADAILVQRGEDSMLVDAGNNDDGEKIIRYLSQQGI
KKLSVVMGTHPHEDHIGGLDDVIKYFPVERVYLPKVNHTTKTYRDVLTAIKNKNLKATAAFGGQTFNLEDARVEILAPNS
SEYKELNDYSIVSKISFGDTAFLLTGDAEMLSEQEMLEKGFDLEANVLKVGHHGSHSSTGDKFLRAVNPQMAVISVGANN
DYGHPHRETLQRLNDSKIKVYQTSQQGTIVMTSDGKRVEVQGEITEAEKAVPKESTANVGNDGKGLIKGNINKNGDKIYH
LPGQQNYDRTKPEVWFKTEQEAQAAGFRRASR

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=252, Percent_Identity=26.1904761904762, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 38788; Mature: 38788

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFIITLLTILLVTTGCSQSKEKPSSTPSVPSGSLRVHFIDVGQADAILVQRGEDSMLV
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEE
DAGNNDDGEKIIRYLSQQGIKKLSVVMGTHPHEDHIGGLDDVIKYFPVERVYLPKVNHTT
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHCCCCEEEECCCCCCH
KTYRDVLTAIKNKNLKATAAFGGQTFNLEDARVEILAPNSSEYKELNDYSIVSKISFGDT
HHHHHHHHHHHCCCCEEEEECCCEEECCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCE
AFLLTGDAEMLSEQEMLEKGFDLEANVLKVGHHGSHSSTGDKFLRAVNPQMAVISVGANN
EEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCC
DYGHPHRETLQRLNDSKIKVYQTSQQGTIVMTSDGKRVEVQGEITEAEKAVPKESTANVG
CCCCCHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEECCHHHHHHCCCCCCCCCCC
NDGKGLIKGNINKNGDKIYHLPGQQNYDRTKPEVWFKTEQEAQAAGFRRASR
CCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKFIITLLTILLVTTGCSQSKEKPSSTPSVPSGSLRVHFIDVGQADAILVQRGEDSMLV
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEE
DAGNNDDGEKIIRYLSQQGIKKLSVVMGTHPHEDHIGGLDDVIKYFPVERVYLPKVNHTT
ECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHCCCCEEEECCCCCCH
KTYRDVLTAIKNKNLKATAAFGGQTFNLEDARVEILAPNSSEYKELNDYSIVSKISFGDT
HHHHHHHHHHHCCCCEEEEECCCEEECCCCCEEEEECCCCHHHHHCCCCEEEEEECCCCE
AFLLTGDAEMLSEQEMLEKGFDLEANVLKVGHHGSHSSTGDKFLRAVNPQMAVISVGANN
EEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCC
DYGHPHRETLQRLNDSKIKVYQTSQQGTIVMTSDGKRVEVQGEITEAEKAVPKESTANVG
CCCCCHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEECCHHHHHHCCCCCCCCCCC
NDGKGLIKGNINKNGDKIYHLPGQQNYDRTKPEVWFKTEQEAQAAGFRRASR
CCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]