Click here to switch to the map view.

The map label for this gene is mrdA [H]

Identifier: 134300384

GI number: 134300384

Start: 2768305

End: 2770281

Strand: Reverse

Name: mrdA [H]

Synonym: Dred_2545

Alternate gene names: 134300384

Gene position: 2770281-2768305 (Counterclockwise)

Preceding gene: 134300385

Following gene: 134300383

Centisome position: 76.78

GC content: 44.76

Gene sequence:

>1977_bases
TTGGAAAGGAAGATGATTGAGAAGAAGACCCGAGTGCTCCTCTTTCTTGTGGTGCTGGTTTTTCTGATCTTGATTGGTCG
TTTAGCCTATATGCAACTTTTAGAAACCCAACGGTTTGAAACCTTATCAAAACAAAATCATATGCGTCTAAATCCTATTA
TTGCTCCTAGGGGGGAGATATTTGACCACAATGGCGAAAAAATTGTGGGAAATAAACCGGTTTACACAGTCTCCCTGGTA
TATCTGGGCCTAAAGGATACCGATAGGGTGGTGAACCGGGTTGCCAGTATATTGAATATGGACCCAAAGGAAATAATGAA
AAAACTTGAAGAGCAACAACTAAGGTTGTTCCAACCGGTTCGTTTGGCCACGGACATTCCTTTGGAAACTGTTTCAATTC
TGGAAGAGCAACGACTGGACTTGCCGGGTGTTGTTATTGACGTAGAACCAGTTAGAAGTTACCCATATAAAGCCATGCTT
TCTCACGTGTTGGGTTATGTACAGGAAATTAAGCAGCACCAGTTAGAAAAAAATCAGGATAAGGGCTACAGACTTGGTGA
TATGTATGGGCAGGATGGTTTGGAGAACGCCTTTGAATCCTATTTAAAGGGTATTGATGGCGCTAGGCAGGTGGAGGTTG
ATGCACAGGCCCGTCCGGTACGGGACCTTGGTATAAAAGAACCGGTCCCGGGTAATAACCTTCATTTAACCATTGATGCT
AATTTGCAAAAAACAGCGGAGACTTCTCTGGAGAGACAAGTACTGGCCATGAGAAAACAGGGGTATGAGGCTAAGGGCGG
TGCCACGGTAATGATTGACGTGAGAACCGGGGCTATCCGGGCCATGGCCAGCTACCCCACCTACGAACCATCCATTTGGA
TTAATGGCCTAAGTCAAAAGCAGTGGGATGAATTACAAAAAAGCAGTGCCTTGCCAAACCGTGCTTTGCAACTGTATCCG
CCAGCCTCTACCTTTAAGATGGTACTGGCGGTGGCAGGGGCGGATACCGGAAAAGTAGATCCCAGCTGGGCTATTTCCGA
CCCGGGACATTATACCTTTGGCAACAGGAATTTTAATGACTGGTTGCTCACTGGGCACGGCAGGGTGGATATGAGAAAGG
CTATTTCAGAGTCCTGTGACACCTATTTCTGGTATCTGGGTCACAGGATACTGGGCGTTGATATCATTGGACAATATGCA
CGAAAGTTTGGCTTTGGGGAGCGTACGGGTATTGAAATACCTGGTGAACCAGCAGGTATTGTACCCACACCAGACTATAA
GTATAAGCGTAACAAAGCCTATTTAGATGCTATTTATGAACCAAAGTTTAAGGCCATAGAGGAAAAATACAAACCATTAC
TGGCCAGTGCTACAGATCCCGATGAAAAGGACAAAATAGAAAAAGCTAAGAAAAAAGAATTAAAACAAGTCCAAAACCAG
TATGATCAATATAAATGGGACTTAAACTGGCAGGTTTATGATACCTTAAACATGTCAATTGGGCAGGGTTATAACCTATA
TACACCTTTGCAAATTGCTAATTATATTGCGGCCATTGCCAATGGTGGAACACGCTGGAAGCCTTACCTGGTGGAAAAAA
TTACAGCTCCAGACGGCAAGATTGTAAAGGAATATAAACCACAAAAAATTGCTGATGTTGGTGCAAAACCTGAGGCAATA
AAAGTGGCCCAGGAAGGAATGCATATGGTAACGACCATTGGGACTGCCTCCGGTATTTTTCGAGGATTTCCCATTGATGT
TGCGGGTAAAACAGGAACTGGGGAAGTCTTTGGCCACGATAACCATGCTTTATTTGTCGCCTATGCGCCCTATGATAAAC
CTGAAGTTGCTGTGGCCACGGTAGTTGACTACGGAGGTCACGGTGGGTCGGCGGCGGGACCTGTGGCAAGGGATTTATTT
GCCACATATTTCAAGGTTGAAAATGTACCACAACAAACGGGCTATAGTCCGGAATAA

Upstream 100 bases:

>100_bases
TCTCAGTTATTGGGAGTTCTATAGTTCCAATGAAAAGGGATGGTTAAGACAACGAGAATTTTAAAATGCTAGAAATATTC
AGAAATAGGAGGAGTCGTCC

Downstream 100 bases:

>100_bases
AACCTCATTTTGTCTAATTATCAAAGAATATCGGGGCTTATAGCCCCTTTTTTTTATCCATCTTTAGAAGGAATTGGATT
TTCTGCGTAGAATTGAAATA

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MERKMIEKKTRVLLFLVVLVFLILIGRLAYMQLLETQRFETLSKQNHMRLNPIIAPRGEIFDHNGEKIVGNKPVYTVSLV
YLGLKDTDRVVNRVASILNMDPKEIMKKLEEQQLRLFQPVRLATDIPLETVSILEEQRLDLPGVVIDVEPVRSYPYKAML
SHVLGYVQEIKQHQLEKNQDKGYRLGDMYGQDGLENAFESYLKGIDGARQVEVDAQARPVRDLGIKEPVPGNNLHLTIDA
NLQKTAETSLERQVLAMRKQGYEAKGGATVMIDVRTGAIRAMASYPTYEPSIWINGLSQKQWDELQKSSALPNRALQLYP
PASTFKMVLAVAGADTGKVDPSWAISDPGHYTFGNRNFNDWLLTGHGRVDMRKAISESCDTYFWYLGHRILGVDIIGQYA
RKFGFGERTGIEIPGEPAGIVPTPDYKYKRNKAYLDAIYEPKFKAIEEKYKPLLASATDPDEKDKIEKAKKKELKQVQNQ
YDQYKWDLNWQVYDTLNMSIGQGYNLYTPLQIANYIAAIANGGTRWKPYLVEKITAPDGKIVKEYKPQKIADVGAKPEAI
KVAQEGMHMVTTIGTASGIFRGFPIDVAGKTGTGEVFGHDNHALFVAYAPYDKPEVAVATVVDYGGHGGSAAGPVARDLF
ATYFKVENVPQQTGYSPE

Sequences:

>Translated_658_residues
MERKMIEKKTRVLLFLVVLVFLILIGRLAYMQLLETQRFETLSKQNHMRLNPIIAPRGEIFDHNGEKIVGNKPVYTVSLV
YLGLKDTDRVVNRVASILNMDPKEIMKKLEEQQLRLFQPVRLATDIPLETVSILEEQRLDLPGVVIDVEPVRSYPYKAML
SHVLGYVQEIKQHQLEKNQDKGYRLGDMYGQDGLENAFESYLKGIDGARQVEVDAQARPVRDLGIKEPVPGNNLHLTIDA
NLQKTAETSLERQVLAMRKQGYEAKGGATVMIDVRTGAIRAMASYPTYEPSIWINGLSQKQWDELQKSSALPNRALQLYP
PASTFKMVLAVAGADTGKVDPSWAISDPGHYTFGNRNFNDWLLTGHGRVDMRKAISESCDTYFWYLGHRILGVDIIGQYA
RKFGFGERTGIEIPGEPAGIVPTPDYKYKRNKAYLDAIYEPKFKAIEEKYKPLLASATDPDEKDKIEKAKKKELKQVQNQ
YDQYKWDLNWQVYDTLNMSIGQGYNLYTPLQIANYIAAIANGGTRWKPYLVEKITAPDGKIVKEYKPQKIADVGAKPEAI
KVAQEGMHMVTTIGTASGIFRGFPIDVAGKTGTGEVFGHDNHALFVAYAPYDKPEVAVATVVDYGGHGGSAAGPVARDLF
ATYFKVENVPQQTGYSPE
>Mature_658_residues
MERKMIEKKTRVLLFLVVLVFLILIGRLAYMQLLETQRFETLSKQNHMRLNPIIAPRGEIFDHNGEKIVGNKPVYTVSLV
YLGLKDTDRVVNRVASILNMDPKEIMKKLEEQQLRLFQPVRLATDIPLETVSILEEQRLDLPGVVIDVEPVRSYPYKAML
SHVLGYVQEIKQHQLEKNQDKGYRLGDMYGQDGLENAFESYLKGIDGARQVEVDAQARPVRDLGIKEPVPGNNLHLTIDA
NLQKTAETSLERQVLAMRKQGYEAKGGATVMIDVRTGAIRAMASYPTYEPSIWINGLSQKQWDELQKSSALPNRALQLYP
PASTFKMVLAVAGADTGKVDPSWAISDPGHYTFGNRNFNDWLLTGHGRVDMRKAISESCDTYFWYLGHRILGVDIIGQYA
RKFGFGERTGIEIPGEPAGIVPTPDYKYKRNKAYLDAIYEPKFKAIEEKYKPLLASATDPDEKDKIEKAKKKELKQVQNQ
YDQYKWDLNWQVYDTLNMSIGQGYNLYTPLQIANYIAAIANGGTRWKPYLVEKITAPDGKIVKEYKPQKIADVGAKPEAI
KVAQEGMHMVTTIGTASGIFRGFPIDVAGKTGTGEVFGHDNHALFVAYAPYDKPEVAVATVVDYGGHGGSAAGPVARDLF
ATYFKVENVPQQTGYSPE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=668, Percent_Identity=27.6946107784431, Blast_Score=255, Evalue=7e-69,
Organism=Escherichia coli, GI1786272, Length=429, Percent_Identity=23.0769230769231, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 73848; Mature: 73848

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKMIEKKTRVLLFLVVLVFLILIGRLAYMQLLETQRFETLSKQNHMRLNPIIAPRGEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEECCCCCE
FDHNGEKIVGNKPVYTVSLVYLGLKDTDRVVNRVASILNMDPKEIMKKLEEQQLRLFQPV
ECCCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCH
RLATDIPLETVSILEEQRLDLPGVVIDVEPVRSYPYKAMLSHVLGYVQEIKQHQLEKNQD
HHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KGYRLGDMYGQDGLENAFESYLKGIDGARQVEVDAQARPVRDLGIKEPVPGNNLHLTIDA
CCCEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCCCCEEEEEEC
NLQKTAETSLERQVLAMRKQGYEAKGGATVMIDVRTGAIRAMASYPTYEPSIWINGLSQK
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCEEECCCCHH
QWDELQKSSALPNRALQLYPPASTFKMVLAVAGADTGKVDPSWAISDPGHYTFGNRNFND
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCCCCC
WLLTGHGRVDMRKAISESCDTYFWYLGHRILGVDIIGQYARKFGFGERTGIEIPGEPAGI
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPTPDYKYKRNKAYLDAIYEPKFKAIEEKYKPLLASATDPDEKDKIEKAKKKELKQVQNQ
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
YDQYKWDLNWQVYDTLNMSIGQGYNLYTPLQIANYIAAIANGGTRWKPYLVEKITAPDGK
HHHEEEECCEEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCC
IVKEYKPQKIADVGAKPEAIKVAQEGMHMVTTIGTASGIFRGFPIDVAGKTGTGEVFGHD
HHHHCCCCHHHHCCCCCHHHHHHHCCCEEEEECCCHHHHHCCCCEEECCCCCCCCEEECC
NHALFVAYAPYDKPEVAVATVVDYGGHGGSAAGPVARDLFATYFKVENVPQQTGYSPE
CCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MERKMIEKKTRVLLFLVVLVFLILIGRLAYMQLLETQRFETLSKQNHMRLNPIIAPRGEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEECCCCCE
FDHNGEKIVGNKPVYTVSLVYLGLKDTDRVVNRVASILNMDPKEIMKKLEEQQLRLFQPV
ECCCCCEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCH
RLATDIPLETVSILEEQRLDLPGVVIDVEPVRSYPYKAMLSHVLGYVQEIKQHQLEKNQD
HHHCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KGYRLGDMYGQDGLENAFESYLKGIDGARQVEVDAQARPVRDLGIKEPVPGNNLHLTIDA
CCCEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCCCCCCCEEEEEEC
NLQKTAETSLERQVLAMRKQGYEAKGGATVMIDVRTGAIRAMASYPTYEPSIWINGLSQK
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCEEECCCCHH
QWDELQKSSALPNRALQLYPPASTFKMVLAVAGADTGKVDPSWAISDPGHYTFGNRNFND
HHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCCCCC
WLLTGHGRVDMRKAISESCDTYFWYLGHRILGVDIIGQYARKFGFGERTGIEIPGEPAGI
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VPTPDYKYKRNKAYLDAIYEPKFKAIEEKYKPLLASATDPDEKDKIEKAKKKELKQVQNQ
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
YDQYKWDLNWQVYDTLNMSIGQGYNLYTPLQIANYIAAIANGGTRWKPYLVEKITAPDGK
HHHEEEECCEEEEEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCC
IVKEYKPQKIADVGAKPEAIKVAQEGMHMVTTIGTASGIFRGFPIDVAGKTGTGEVFGHD
HHHHCCCCHHHHCCCCCHHHHHHHCCCEEEEECCCHHHHHCCCCEEECCCCCCCCEEECC
NHALFVAYAPYDKPEVAVATVVDYGGHGGSAAGPVARDLFATYFKVENVPQQTGYSPE
CCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]