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The map label for this gene is rng [H]

Identifier: 134300375

GI number: 134300375

Start: 2758683

End: 2760362

Strand: Reverse

Name: rng [H]

Synonym: Dred_2536

Alternate gene names: 134300375

Gene position: 2760362-2758683 (Counterclockwise)

Preceding gene: 134300376

Following gene: 134300374

Centisome position: 76.5

GC content: 43.15

Gene sequence:

>1680_bases
ATGTTAAAAGAAATTGTTATTAATGTTCAAGAAGAGGAAACACGGGTTGCCGTACTGGAAGACCGGGTGTTGATGGAAGT
GTACATAGAGCGTTCTCACAACCAACGTCTGGTAGGCAACATTTTTAAAGGAAAAGTAGAAAACGTTCTACCCGGTATGC
AGGCTGCCTTTGTTGATATTGGTTTAGAAAAAAATGCTTTTCTTTATGTAGAAGATGCCCAGCCATCCAGGAACCCTGAA
GCAGCCAATCAATCAGGCTATCCCGTAAATATTGCTGATATTTTAAAACAGGGTCAAGAAATTATTGTTCAAATTGTTAA
GGAACCTATTGGTACCAAGGGGCCCAGGGTAACTACCCATATAACCTTGCCAGGCAGGTACTTAGTCCTCATGCCCACCG
TTGACTACATTGGTATTTCCCGTCGTATTGAGACAGAAAAAGAACGGGAGAGGTTAAAGGAGTTAGCCAACAGAGTAAAG
CCCGAGGGGATGGGGGTCATTGTTAGAACGGTGGCTGAAGGGGTGGATGAAGAGGAGTTTTTGCAAGATATTATTTTGCT
GACAAAACTTTGGCGAAGAATTCAGAACTGTGCAGCCAACTGTTCTGCACCTAACCTATTGCATCGAGACGTGGAATTGG
TGCAAAGGATGTTACGAGACGTTTTTAGTGAAGATGTTGATCGTTTAACGGTGGATTCTCGTAGTGAGTATGAGAAGACT
CTGGAAATCCTAGATATGATTGATTCCCGCTTAAAGCTGAAGGTTTTTTTGGATGAGCGTGAAAATATTTTTGAGGACTA
TGGAATATCCACTGAAATGGAAAAGGCACTTAAAAGGAAAGTATGGTTAAAATGTGGTGCCTACTTAGTAATAGATCAAG
CCGAGGCCCTGACGGCCATTGATGTAAATACAGGGAAGTTCGTAGGCAGTACCACCCTAGAAGATACTGTGCTAAAAACT
AATCTAGATGCTGCTGTAGAAATTGCCCGTCAACTTCGTTTGAGAAATATTGGTGGTATTATCATTGTTGATTTTATCGA
TATGGTTGAAGAAGAACACCGGAGTCAAGTATTAAGCACCCTAGAAGAGGAAATAAAAAAAGACAAAACAAAAACCAATA
TTTTAGGAATCACACAACTGGGTTTAGTGGAAATGACCCGAAAAAAGGTGCGTCCATCTCTGGCAGAAGTGGTTCAAAAA
TCTTGCCCCTATTGTGAAGGTCGAGGTAAGGTGCTATCTGAAGAAACCGTTGGCATTAAGCTAAAGAACCAGATTTATCA
ATTGGCGCGCCAAACACTGGCTGATACCATTTTAGTTGAGGCGAACCCCGTTGTGGCTGCTAGAATCATTGGGGCTGGTG
GGGCCAACCTACGGGAATTGGAGCAGAAAACTGGCAAGAATCTTTATATTCGAGGTTCTGCCACTCATCACCTTGAACAA
GTCACTATCAAGCCTCTTTCAGACCAAGAAGAAATCTACTCTCAAACGGTCCCTGTAAAGGCTGGTGAAATTATTGAGGT
CAAAGTGGAAGAACCCCACATATCCAATCCCAGTGACGGTATCGCTCGCCTAGATGGTTTTATTATTGATGTGGATGGGG
GAGGGGCCCTGGTGGGAGAACTGGTCTCAGTGGAGGTAATCAAGGTGCACCGGACCTATGCAAAGGCACGTTTGGTTTGA

Upstream 100 bases:

>100_bases
GATTACCGGGTACGTCGTACGGCCCTGTACGCCGATGACGGCACTGGCCCGATTTCTCTCTGGGAGGTCTAATGGCCAGC
AGTAAGAGAGGAAGTTCAAA

Downstream 100 bases:

>100_bases
TATCCATGAAGACTGGAAGACAGAATGACATTTTGAACTAAATATAGGCTTATAAACTGTAGAGGTGAGAGGCGAGAAGT
GAGATTTTCTAACCTCTCAC

Product: ribonuclease

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLKEIVINVQEEETRVAVLEDRVLMEVYIERSHNQRLVGNIFKGKVENVLPGMQAAFVDIGLEKNAFLYVEDAQPSRNPE
AANQSGYPVNIADILKQGQEIIVQIVKEPIGTKGPRVTTHITLPGRYLVLMPTVDYIGISRRIETEKERERLKELANRVK
PEGMGVIVRTVAEGVDEEEFLQDIILLTKLWRRIQNCAANCSAPNLLHRDVELVQRMLRDVFSEDVDRLTVDSRSEYEKT
LEILDMIDSRLKLKVFLDERENIFEDYGISTEMEKALKRKVWLKCGAYLVIDQAEALTAIDVNTGKFVGSTTLEDTVLKT
NLDAAVEIARQLRLRNIGGIIIVDFIDMVEEEHRSQVLSTLEEEIKKDKTKTNILGITQLGLVEMTRKKVRPSLAEVVQK
SCPYCEGRGKVLSEETVGIKLKNQIYQLARQTLADTILVEANPVVAARIIGAGGANLRELEQKTGKNLYIRGSATHHLEQ
VTIKPLSDQEEIYSQTVPVKAGEIIEVKVEEPHISNPSDGIARLDGFIIDVDGGGALVGELVSVEVIKVHRTYAKARLV

Sequences:

>Translated_559_residues
MLKEIVINVQEEETRVAVLEDRVLMEVYIERSHNQRLVGNIFKGKVENVLPGMQAAFVDIGLEKNAFLYVEDAQPSRNPE
AANQSGYPVNIADILKQGQEIIVQIVKEPIGTKGPRVTTHITLPGRYLVLMPTVDYIGISRRIETEKERERLKELANRVK
PEGMGVIVRTVAEGVDEEEFLQDIILLTKLWRRIQNCAANCSAPNLLHRDVELVQRMLRDVFSEDVDRLTVDSRSEYEKT
LEILDMIDSRLKLKVFLDERENIFEDYGISTEMEKALKRKVWLKCGAYLVIDQAEALTAIDVNTGKFVGSTTLEDTVLKT
NLDAAVEIARQLRLRNIGGIIIVDFIDMVEEEHRSQVLSTLEEEIKKDKTKTNILGITQLGLVEMTRKKVRPSLAEVVQK
SCPYCEGRGKVLSEETVGIKLKNQIYQLARQTLADTILVEANPVVAARIIGAGGANLRELEQKTGKNLYIRGSATHHLEQ
VTIKPLSDQEEIYSQTVPVKAGEIIEVKVEEPHISNPSDGIARLDGFIIDVDGGGALVGELVSVEVIKVHRTYAKARLV
>Mature_559_residues
MLKEIVINVQEEETRVAVLEDRVLMEVYIERSHNQRLVGNIFKGKVENVLPGMQAAFVDIGLEKNAFLYVEDAQPSRNPE
AANQSGYPVNIADILKQGQEIIVQIVKEPIGTKGPRVTTHITLPGRYLVLMPTVDYIGISRRIETEKERERLKELANRVK
PEGMGVIVRTVAEGVDEEEFLQDIILLTKLWRRIQNCAANCSAPNLLHRDVELVQRMLRDVFSEDVDRLTVDSRSEYEKT
LEILDMIDSRLKLKVFLDERENIFEDYGISTEMEKALKRKVWLKCGAYLVIDQAEALTAIDVNTGKFVGSTTLEDTVLKT
NLDAAVEIARQLRLRNIGGIIIVDFIDMVEEEHRSQVLSTLEEEIKKDKTKTNILGITQLGLVEMTRKKVRPSLAEVVQK
SCPYCEGRGKVLSEETVGIKLKNQIYQLARQTLADTILVEANPVVAARIIGAGGANLRELEQKTGKNLYIRGSATHHLEQ
VTIKPLSDQEEIYSQTVPVKAGEIIEVKVEEPHISNPSDGIARLDGFIIDVDGGGALVGELVSVEVIKVHRTYAKARLV

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=495, Percent_Identity=44.4444444444444, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1787325, Length=425, Percent_Identity=38.8235294117647, Blast_Score=295, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 62717; Mature: 62717

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1 ; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEIVINVQEEETRVAVLEDRVLMEVYIERSHNQRLVGNIFKGKVENVLPGMQAAFVDI
CCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHEEE
GLEKNAFLYVEDAQPSRNPEAANQSGYPVNIADILKQGQEIIVQIVKEPIGTKGPRVTTH
CCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ITLPGRYLVLMPTVDYIGISRRIETEKERERLKELANRVKPEGMGVIVRTVAEGVDEEEF
EECCCEEEEEEECHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
LQDIILLTKLWRRIQNCAANCSAPNLLHRDVELVQRMLRDVFSEDVDRLTVDSRSEYEKT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHH
LEILDMIDSRLKLKVFLDERENIFEDYGISTEMEKALKRKVWLKCGAYLVIDQAEALTAI
HHHHHHHCCCEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEE
DVNTGKFVGSTTLEDTVLKTNLDAAVEIARQLRLRNIGGIIIVDFIDMVEEEHRSQVLST
ECCCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
LEEEIKKDKTKTNILGITQLGLVEMTRKKVRPSLAEVVQKSCPYCEGRGKVLSEETVGIK
HHHHHHHCCHHHHEEEHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEECCCCCCEE
LKNQIYQLARQTLADTILVEANPVVAARIIGAGGANLRELEQKTGKNLYIRGSATHHLEQ
HHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCHHHHHHHHCCEEEEECCCHHCCCE
VTIKPLSDQEEIYSQTVPVKAGEIIEVKVEEPHISNPSDGIARLDGFIIDVDGGGALVGE
EEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCEEEEECCCCHHHHH
LVSVEVIKVHRTYAKARLV
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKEIVINVQEEETRVAVLEDRVLMEVYIERSHNQRLVGNIFKGKVENVLPGMQAAFVDI
CCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHEEE
GLEKNAFLYVEDAQPSRNPEAANQSGYPVNIADILKQGQEIIVQIVKEPIGTKGPRVTTH
CCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ITLPGRYLVLMPTVDYIGISRRIETEKERERLKELANRVKPEGMGVIVRTVAEGVDEEEF
EECCCEEEEEEECHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
LQDIILLTKLWRRIQNCAANCSAPNLLHRDVELVQRMLRDVFSEDVDRLTVDSRSEYEKT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHH
LEILDMIDSRLKLKVFLDERENIFEDYGISTEMEKALKRKVWLKCGAYLVIDQAEALTAI
HHHHHHHCCCEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEEE
DVNTGKFVGSTTLEDTVLKTNLDAAVEIARQLRLRNIGGIIIVDFIDMVEEEHRSQVLST
ECCCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
LEEEIKKDKTKTNILGITQLGLVEMTRKKVRPSLAEVVQKSCPYCEGRGKVLSEETVGIK
HHHHHHHCCHHHHEEEHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCEECCCCCCEE
LKNQIYQLARQTLADTILVEANPVVAARIIGAGGANLRELEQKTGKNLYIRGSATHHLEQ
HHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCHHHHHHHHCCEEEEECCCHHCCCE
VTIKPLSDQEEIYSQTVPVKAGEIIEVKVEEPHISNPSDGIARLDGFIIDVDGGGALVGE
EEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCEEEEECCCCHHHHH
LVSVEVIKVHRTYAKARLV
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]