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The map label for this gene is ppdK [H]

Identifier: 134300308

GI number: 134300308

Start: 2689389

End: 2692046

Strand: Reverse

Name: ppdK [H]

Synonym: Dred_2469

Alternate gene names: 134300308

Gene position: 2692046-2689389 (Counterclockwise)

Preceding gene: 134300309

Following gene: 134300307

Centisome position: 74.61

GC content: 46.43

Gene sequence:

>2658_bases
TTGGCAACCCAAAAATACGTGTATGCTTTTTCCGAGGGAAGAGCAGATATGAAAAGCCTGTTAGGCGGTAAAGGCGCCAA
CCTGGCAGAGATGACCAACATTGGTCTTCCGGTGCCCCCTGGTTTTACCATTACCACCGAGGCTTGTAAAGAGTTTTACA
TAGCCGGACGTAAATTCCCCGAGGGACTTGAGCAGGAACTAGAAACAAAGGTAGACTGGTTAGAGCAAACCCTGAATAAA
AAATTTGGTGATCCCAATGGTCCCCTTCTGGTGTCTGTACGTTCTGGTGCAGTTATATCCATGCCTGGTATGATGGATAC
GGTTTTAAACCTAGGACTTAATGACCAAACTGTCATTGGTTTGGCTACCAGCAGTGGCAATGCTCGCTGGGCTTATGATT
GCTATCGTCGCTTTATACAAATGTTTGGTAATGTGGTGCTAGACATCGAACATCATAAGTTTGAAGGCATCTTAGAAGAG
CAAAAGCATAAAAGAAATGTTCAGTTTGACAATCAGTTGACTTCCGAAGACTGGCAGGAAGTTATAGGAAAATATAAAGC
TATTGTTCAGAAAGAAACCGGTCGTGAATTTCCTCAGGATCCTAAGGAACAGCTACACAAAGCCATTTTTGCTGTCTTTA
ATTCCTGGGAAAATGACCGTGCCATCGTGTATCGTAAAATTCATAAAATCTCCGATGACCTGGGTACAGCAGTAAACGTA
CAGGCAATGGTATTTGGCAACATGGGGGATGATTGTGGTACCGGTGTAGCCTTTACCCGCAATCCTTCCACAGGCGAAAA
ACTACTCTATGGTGAGTTTTTAACCAATGCCCAGGGTGAAGATGTAGTGGCTGGTATTCGTACGCCTCAGCCCATTGCAA
AACTGGCCGATGAAATGCCAGATGTTTACCAACAGTTTGCCCGTACTTGTACTTTATTGGAAAACCACTATAAAGATATG
CAGGATATTGAATTTACCATTGAACGCAACAAGTTATGGATGCTGCAGACCCGTAACGGCAAGCGTACTGCCCAGGCTGC
CATTCGCATTGCGGTGGAAATGGTAGAAGAGGGTATCATTGATAAAGAAATGGCCATCAACCGTGTTGATCCCAATCAGT
TAGACCAACTGTTACACCGTCGCATTGACCCCAACGCTAAATTAGAAGTTGTAGCCAAAGGTCTGCCGGCATCCCCTGGT
TCCGCATCTGGTGCTGTGGTCTTTAATGCTGATCTGGCTGAAAAATTAAACAAACAGGGACAAAAAGTGATCTTAGTGGG
TACTGAAACCACTCCCGACGACATCCATGGTATGGTGGCTGCTCAGGGTATTCTAACTTCCAGGGGCGGCATGACCAGCC
ACGCTGCTGTGGTGGCCCGTGGTATGGGTAAACCCTGTGTGTGTGGTTGCGAAGCCATTCGCATTGACTCTGAAAATAAA
CAGTTTTTTGTAGGCAATATTACCGTTAAAAATGGGGACATTGTATCCATTGATGGTTCCACCGGTGATGTTATCCTGGG
TGAAGTGCCCATGATTGAGCCGGATCTATCGGCGGAATTCTTGAAATTGTTGCAATGGGCTGACAACATCCGTACTTTGG
AAGTACGCGCCAATGCCGATACCCCGGAAGATGCTGCAAAGGCTCGGGAATTCGGTGCCCAAGGTATTGGTTTAACCAGA
ACTGAGCATATGTTTATGGCATTGGACCGTTTACCCATTGTGCAAGAAATGATTTTAGCCAATGATATTGAGGAGCGTGC
AGCAGCACTGGCTAAATTACTGCCGATGCAAGAAGGGGACTTCTACGGTATCCTGAAGGCCATGGAAGGCCTGCCGGTAT
GTATTCGACTATTAGACCCCCCGTTGCATGAATTCTTGCCCAATGCAGAAGAACTGGCTGTGGAAATTACTCAGTTGAAG
TTGACCAACGGTGAACCGGCTAAAATACAGCAAAAGGAAGATTTGTTACGTAAAGTACGTTCCTTGTCTGAATTTAACCC
CATGCTGGGCCATCGGGGTTGCCGTCTAGGTATTACCTGGCCGGAAGTATACGCCATGCAGGCCAGAGCCATCTTCCAAG
CAACTGCTCGTCTGGTGAAAGAAGATTGCAAGATAGATCCAGAAGTTGAAATTCCTTTGGTTATTGAACTAAAAGAATTA
GCATACCTGCGTAAATTGGTGGAAGATGTTGCTGAGGAAGTTAAGAAGGAAACCGGTGTGGACTTCCATTATAGTGTGGG
AACCATGATTGAAATTCCCAGGGCAGCTTTACTGGCCGATGAAATTGCTAGCCAGGCAGAATTCTTCTCCTTCGGTACGA
ATGACTTGACTCAAACCACCCTGGGCTTTTCCAGAGATGACGCAGAGGGCAAATTTATGCAGCCCTATATTGAAAAGAAA
ATCCTTAACGATAACCCCTTCATTGTATTAGATCGTAAGGGTGTTGGTAAGCTTATGCAGTGGACTGTGGAAAATGCCCG
TCGGGTGAAACCAGATCTGCTCATTGGTATCTGCGGTGAGCACGGCGGCGAACCCAGCTCTGTGGAGTTTTGCCACATGA
TTGGGTTGAACTATGTAAGCTGCTCCCCCTACCGGGTACCCATTGCACGGTTGGCAGCCGCCCAGGCGGCTGTAATCAAC
AAAGACCAATCGAAGTAA

Upstream 100 bases:

>100_bases
AAGAGGTGGGGTCTAAGGGGTGAGGAATTCTTTCTCTCACCTCACCACCTTGAAGGTATGATTTTACTGACATGATAAAA
AACAGGAGGGAATTGAAAAA

Downstream 100 bases:

>100_bases
GGCTTAAAATGATATAGGCAAAGCTCTCAAGACCGAGATACGTACAAAAATTAGGAGTGTCTGGTTCTAGGTAATGTTAC
CCGCCGATAAAGGTACTGAA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 885; Mature: 884

Protein sequence:

>885_residues
MATQKYVYAFSEGRADMKSLLGGKGANLAEMTNIGLPVPPGFTITTEACKEFYIAGRKFPEGLEQELETKVDWLEQTLNK
KFGDPNGPLLVSVRSGAVISMPGMMDTVLNLGLNDQTVIGLATSSGNARWAYDCYRRFIQMFGNVVLDIEHHKFEGILEE
QKHKRNVQFDNQLTSEDWQEVIGKYKAIVQKETGREFPQDPKEQLHKAIFAVFNSWENDRAIVYRKIHKISDDLGTAVNV
QAMVFGNMGDDCGTGVAFTRNPSTGEKLLYGEFLTNAQGEDVVAGIRTPQPIAKLADEMPDVYQQFARTCTLLENHYKDM
QDIEFTIERNKLWMLQTRNGKRTAQAAIRIAVEMVEEGIIDKEMAINRVDPNQLDQLLHRRIDPNAKLEVVAKGLPASPG
SASGAVVFNADLAEKLNKQGQKVILVGTETTPDDIHGMVAAQGILTSRGGMTSHAAVVARGMGKPCVCGCEAIRIDSENK
QFFVGNITVKNGDIVSIDGSTGDVILGEVPMIEPDLSAEFLKLLQWADNIRTLEVRANADTPEDAAKAREFGAQGIGLTR
TEHMFMALDRLPIVQEMILANDIEERAAALAKLLPMQEGDFYGILKAMEGLPVCIRLLDPPLHEFLPNAEELAVEITQLK
LTNGEPAKIQQKEDLLRKVRSLSEFNPMLGHRGCRLGITWPEVYAMQARAIFQATARLVKEDCKIDPEVEIPLVIELKEL
AYLRKLVEDVAEEVKKETGVDFHYSVGTMIEIPRAALLADEIASQAEFFSFGTNDLTQTTLGFSRDDAEGKFMQPYIEKK
ILNDNPFIVLDRKGVGKLMQWTVENARRVKPDLLIGICGEHGGEPSSVEFCHMIGLNYVSCSPYRVPIARLAAAQAAVIN
KDQSK

Sequences:

>Translated_885_residues
MATQKYVYAFSEGRADMKSLLGGKGANLAEMTNIGLPVPPGFTITTEACKEFYIAGRKFPEGLEQELETKVDWLEQTLNK
KFGDPNGPLLVSVRSGAVISMPGMMDTVLNLGLNDQTVIGLATSSGNARWAYDCYRRFIQMFGNVVLDIEHHKFEGILEE
QKHKRNVQFDNQLTSEDWQEVIGKYKAIVQKETGREFPQDPKEQLHKAIFAVFNSWENDRAIVYRKIHKISDDLGTAVNV
QAMVFGNMGDDCGTGVAFTRNPSTGEKLLYGEFLTNAQGEDVVAGIRTPQPIAKLADEMPDVYQQFARTCTLLENHYKDM
QDIEFTIERNKLWMLQTRNGKRTAQAAIRIAVEMVEEGIIDKEMAINRVDPNQLDQLLHRRIDPNAKLEVVAKGLPASPG
SASGAVVFNADLAEKLNKQGQKVILVGTETTPDDIHGMVAAQGILTSRGGMTSHAAVVARGMGKPCVCGCEAIRIDSENK
QFFVGNITVKNGDIVSIDGSTGDVILGEVPMIEPDLSAEFLKLLQWADNIRTLEVRANADTPEDAAKAREFGAQGIGLTR
TEHMFMALDRLPIVQEMILANDIEERAAALAKLLPMQEGDFYGILKAMEGLPVCIRLLDPPLHEFLPNAEELAVEITQLK
LTNGEPAKIQQKEDLLRKVRSLSEFNPMLGHRGCRLGITWPEVYAMQARAIFQATARLVKEDCKIDPEVEIPLVIELKEL
AYLRKLVEDVAEEVKKETGVDFHYSVGTMIEIPRAALLADEIASQAEFFSFGTNDLTQTTLGFSRDDAEGKFMQPYIEKK
ILNDNPFIVLDRKGVGKLMQWTVENARRVKPDLLIGICGEHGGEPSSVEFCHMIGLNYVSCSPYRVPIARLAAAQAAVIN
KDQSK
>Mature_884_residues
ATQKYVYAFSEGRADMKSLLGGKGANLAEMTNIGLPVPPGFTITTEACKEFYIAGRKFPEGLEQELETKVDWLEQTLNKK
FGDPNGPLLVSVRSGAVISMPGMMDTVLNLGLNDQTVIGLATSSGNARWAYDCYRRFIQMFGNVVLDIEHHKFEGILEEQ
KHKRNVQFDNQLTSEDWQEVIGKYKAIVQKETGREFPQDPKEQLHKAIFAVFNSWENDRAIVYRKIHKISDDLGTAVNVQ
AMVFGNMGDDCGTGVAFTRNPSTGEKLLYGEFLTNAQGEDVVAGIRTPQPIAKLADEMPDVYQQFARTCTLLENHYKDMQ
DIEFTIERNKLWMLQTRNGKRTAQAAIRIAVEMVEEGIIDKEMAINRVDPNQLDQLLHRRIDPNAKLEVVAKGLPASPGS
ASGAVVFNADLAEKLNKQGQKVILVGTETTPDDIHGMVAAQGILTSRGGMTSHAAVVARGMGKPCVCGCEAIRIDSENKQ
FFVGNITVKNGDIVSIDGSTGDVILGEVPMIEPDLSAEFLKLLQWADNIRTLEVRANADTPEDAAKAREFGAQGIGLTRT
EHMFMALDRLPIVQEMILANDIEERAAALAKLLPMQEGDFYGILKAMEGLPVCIRLLDPPLHEFLPNAEELAVEITQLKL
TNGEPAKIQQKEDLLRKVRSLSEFNPMLGHRGCRLGITWPEVYAMQARAIFQATARLVKEDCKIDPEVEIPLVIELKELA
YLRKLVEDVAEEVKKETGVDFHYSVGTMIEIPRAALLADEIASQAEFFSFGTNDLTQTTLGFSRDDAEGKFMQPYIEKKI
LNDNPFIVLDRKGVGKLMQWTVENARRVKPDLLIGICGEHGGEPSSVEFCHMIGLNYVSCSPYRVPIARLAAAQAAVINK
DQSK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=467, Percent_Identity=25.9100642398287, Blast_Score=123, Evalue=6e-29,
Organism=Escherichia coli, GI1787994, Length=711, Percent_Identity=24.4725738396624, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1789193, Length=473, Percent_Identity=23.6786469344609, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI48994992, Length=475, Percent_Identity=23.5789473684211, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788726, Length=458, Percent_Identity=22.707423580786, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 98105; Mature: 97974

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQKYVYAFSEGRADMKSLLGGKGANLAEMTNIGLPVPPGFTITTEACKEFYIAGRKFP
CCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCC
EGLEQELETKVDWLEQTLNKKFGDPNGPLLVSVRSGAVISMPGMMDTVLNLGLNDQTVIG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCHHHHHHHCCCCCCEEEE
LATSSGNARWAYDCYRRFIQMFGNVVLDIEHHKFEGILEEQKHKRNVQFDNQLTSEDWQE
EEECCCCCEEHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VIGKYKAIVQKETGREFPQDPKEQLHKAIFAVFNSWENDRAIVYRKIHKISDDLGTAVNV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEE
QAMVFGNMGDDCGTGVAFTRNPSTGEKLLYGEFLTNAQGEDVVAGIRTPQPIAKLADEMP
EEEEECCCCCCCCCCCEEECCCCCCCEEEEHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
DVYQQFARTCTLLENHYKDMQDIEFTIERNKLWMLQTRNGKRTAQAAIRIAVEMVEEGII
HHHHHHHHHHHHHHHHCCCHHHEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
DKEMAINRVDPNQLDQLLHRRIDPNAKLEVVAKGLPASPGSASGAVVFNADLAEKLNKQG
CHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECHHHHHHHCCC
QKVILVGTETTPDDIHGMVAAQGILTSRGGMTSHAAVVARGMGKPCVCGCEAIRIDSENK
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCEEEEECCCCC
QFFVGNITVKNGDIVSIDGSTGDVILGEVPMIEPDLSAEFLKLLQWADNIRTLEVRANAD
EEEEEEEEEECCCEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCC
TPEDAAKAREFGAQGIGLTRTEHMFMALDRLPIVQEMILANDIEERAAALAKLLPMQEGD
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
FYGILKAMEGLPVCIRLLDPPLHEFLPNAEELAVEITQLKLTNGEPAKIQQKEDLLRKVR
HHHHHHHHCCCHHHHHHCCCCHHHHCCCHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHH
SLSEFNPMLGHRGCRLGITWPEVYAMQARAIFQATARLVKEDCKIDPEVEIPLVIELKEL
HHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHH
AYLRKLVEDVAEEVKKETGVDFHYSVGTMIEIPRAALLADEIASQAEFFSFGTNDLTQTT
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
LGFSRDDAEGKFMQPYIEKKILNDNPFIVLDRKGVGKLMQWTVENARRVKPDLLIGICGE
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCC
HGGEPSSVEFCHMIGLNYVSCSPYRVPIARLAAAQAAVINKDQSK
CCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ATQKYVYAFSEGRADMKSLLGGKGANLAEMTNIGLPVPPGFTITTEACKEFYIAGRKFP
CCCEEEEEECCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCC
EGLEQELETKVDWLEQTLNKKFGDPNGPLLVSVRSGAVISMPGMMDTVLNLGLNDQTVIG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEECCCHHHHHHHCCCCCCEEEE
LATSSGNARWAYDCYRRFIQMFGNVVLDIEHHKFEGILEEQKHKRNVQFDNQLTSEDWQE
EEECCCCCEEHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VIGKYKAIVQKETGREFPQDPKEQLHKAIFAVFNSWENDRAIVYRKIHKISDDLGTAVNV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEE
QAMVFGNMGDDCGTGVAFTRNPSTGEKLLYGEFLTNAQGEDVVAGIRTPQPIAKLADEMP
EEEEECCCCCCCCCCCEEECCCCCCCEEEEHHHHCCCCCCCEEEECCCCHHHHHHHHHHH
DVYQQFARTCTLLENHYKDMQDIEFTIERNKLWMLQTRNGKRTAQAAIRIAVEMVEEGII
HHHHHHHHHHHHHHHHCCCHHHEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
DKEMAINRVDPNQLDQLLHRRIDPNAKLEVVAKGLPASPGSASGAVVFNADLAEKLNKQG
CHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECHHHHHHHCCC
QKVILVGTETTPDDIHGMVAAQGILTSRGGMTSHAAVVARGMGKPCVCGCEAIRIDSENK
CEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCEEEEECCCCC
QFFVGNITVKNGDIVSIDGSTGDVILGEVPMIEPDLSAEFLKLLQWADNIRTLEVRANAD
EEEEEEEEEECCCEEEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCC
TPEDAAKAREFGAQGIGLTRTEHMFMALDRLPIVQEMILANDIEERAAALAKLLPMQEGD
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
FYGILKAMEGLPVCIRLLDPPLHEFLPNAEELAVEITQLKLTNGEPAKIQQKEDLLRKVR
HHHHHHHHCCCHHHHHHCCCCHHHHCCCHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHH
SLSEFNPMLGHRGCRLGITWPEVYAMQARAIFQATARLVKEDCKIDPEVEIPLVIELKEL
HHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHH
AYLRKLVEDVAEEVKKETGVDFHYSVGTMIEIPRAALLADEIASQAEFFSFGTNDLTQTT
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
LGFSRDDAEGKFMQPYIEKKILNDNPFIVLDRKGVGKLMQWTVENARRVKPDLLIGICGE
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCC
HGGEPSSVEFCHMIGLNYVSCSPYRVPIARLAAAQAAVINKDQSK
CCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]