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The map label for this gene is nudG [H]

Identifier: 134300286

GI number: 134300286

Start: 2662866

End: 2663255

Strand: Reverse

Name: nudG [H]

Synonym: Dred_2447

Alternate gene names: 134300286

Gene position: 2663255-2662866 (Counterclockwise)

Preceding gene: 134300287

Following gene: 134300285

Centisome position: 73.81

GC content: 45.9

Gene sequence:

>390_bases
ATGCACATCCTTGTTGTCACCGCAGCTATTATTCATAGAGAAGATAAAATATTAATTGCTCAGCGAAAAAGTAGTGCTGA
GCATGGCCTGAAATGGGAGTTTCCCGGGGGAAAGCTAAACTATGGAGAGGACCCAAAGGATGGCCTTCGTAGAGAGATTA
TCGAAGAACTGGACATGGAAATCCAGGTGGGAGATATTTTTGAAGTGATTTCCCACCACTATGGGGAAAGGCACATTTTG
CTTTTGTGTTACCATTGTGGCTACCTGGGGCAACGGCCCAGCACACGGGACTGTCAGGATTTTCGATGGGTAACACCCGC
AGAGATGGCCCAGTATGACTTTAGTGAAGCGGATGTACCCGTTGTAAAAAAACTCCAGGGTATAAAATAG

Upstream 100 bases:

>100_bases
TCCTAGCCTCAGCTGTGGTAAAACAATGGCACTAAGGTTGTAATATTGGTTTAACAGTGGCACAATAGATAAAGAACAGA
ATACCATTAGGAGATAAAAA

Downstream 100 bases:

>100_bases
ACCGGGATATAGTTTGGGGTACTGTTCCTAAAAGGGGAGATCGGTTTGTTGAAACAAGTTGTATTCCCTGAATTTCTGGG
GGAATCTGAAATAGCAGTGG

Product: NUDIX hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MHILVVTAAIIHREDKILIAQRKSSAEHGLKWEFPGGKLNYGEDPKDGLRREIIEELDMEIQVGDIFEVISHHYGERHIL
LLCYHCGYLGQRPSTRDCQDFRWVTPAEMAQYDFSEADVPVVKKLQGIK

Sequences:

>Translated_129_residues
MHILVVTAAIIHREDKILIAQRKSSAEHGLKWEFPGGKLNYGEDPKDGLRREIIEELDMEIQVGDIFEVISHHYGERHIL
LLCYHCGYLGQRPSTRDCQDFRWVTPAEMAQYDFSEADVPVVKKLQGIK
>Mature_129_residues
MHILVVTAAIIHREDKILIAQRKSSAEHGLKWEFPGGKLNYGEDPKDGLRREIIEELDMEIQVGDIFEVISHHYGERHIL
LLCYHCGYLGQRPSTRDCQDFRWVTPAEMAQYDFSEADVPVVKKLQGIK

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=129, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1786288, Length=127, Percent_Identity=31.496062992126, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 14889; Mature: 14889

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILVVTAAIIHREDKILIAQRKSSAEHGLKWEFPGGKLNYGEDPKDGLRREIIEELDME
CEEEEEEHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
IQVGDIFEVISHHYGERHILLLCYHCGYLGQRPSTRDCQDFRWVTPAEMAQYDFSEADVP
CEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCH
VVKKLQGIK
HHHHHCCCC
>Mature Secondary Structure
MHILVVTAAIIHREDKILIAQRKSSAEHGLKWEFPGGKLNYGEDPKDGLRREIIEELDME
CEEEEEEHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCC
IQVGDIFEVISHHYGERHILLLCYHCGYLGQRPSTRDCQDFRWVTPAEMAQYDFSEADVP
CEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCH
VVKKLQGIK
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]