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The map label for this gene is fliF [H]

Identifier: 134300247

GI number: 134300247

Start: 2628811

End: 2630376

Strand: Reverse

Name: fliF [H]

Synonym: Dred_2408

Alternate gene names: 134300247

Gene position: 2630376-2628811 (Counterclockwise)

Preceding gene: 134300248

Following gene: 134300246

Centisome position: 72.9

GC content: 46.1

Gene sequence:

>1566_bases
GTGAACTTTAATGACCTGCTGGAGAGACTTAAACAGCGGTGGCAGGAAACAAGTCAAAACAGAAAAGTATTGGTCATCCT
GGTAAGCACAGTTGTTCTGGCAGGTTTGGTGTATTTGGCAATGACATTGACAAAACCCAATTATGCAATTTTGCTGGGAG
ACTTGGAACCAAAGGATGCCGGAGATATTGTTGCACAACTGGAAAGCGAAAAGATTCCTTACCAGTTAATTGATCAAGGA
ACAACCATTCTCGTTCCCCAGGAGCAAGTGGACGAGATTCGGGTGAAGTTAGCCAGTGATGGCTTGCTGACAGGAGTGGG
GAATGGCTTTGAGCTATTTGATAAAAGTAAATTCGGAGAAACGGATTTCGAACAACAAATATCCTATCAGCGGGCTCTTC
AAGAGGAACTAAGACGTACTATTACTAAGGTGGAGGGTGTTGAACAAGCCCGGGTACACCTGGTATTGCCCCGGAAAAGT
GTTTTTATTGAGGAGGAGGGAACTGCTTCGGCCTCGGTGGTGGTAAAATTGAAGCCCCGGGCTAAACTGAAAGCGGAACA
GGTCAAGGGCCTTAATGACCTGATTATCGGCAGCGTAGAAGGTTTGCAAGCGGAGAACGTACATATCATTGACACCGAAG
GAAATGTCTTAAACGATTTTCTTAAGGATTCTTCCGGGACCCCTGGGAATGGCTTTTCTGGAACAATTTTGGAACGACAA
CAGCAGATTCGCCGGACCTACGAAAAAGAATTAGAGAACCGGGTACAAAATATGCTGGCCAAGGTATTGGGACCCAATAA
AGCAGTGGCTATGGTTACAGCAGAAATGGATTTTAACCAACTGCAAACCAATACCACTGAGGTTGTGCCAGGTCAAGTGG
TCAGCGAGAGAAGTATCAAGGAACAAGGCAGTGATGGTAGTGGCGGCGGGGTGACTGGTTCAACCACGCAAATGCCCGGT
CAGAGTATTCCCGCCGAGACTTCAACCAGTCAAAATAGTTATATCAAGACCGATGACACCAAGAATTACCAACATGGAAC
CAGAACCCAATCAGAGGTGCAGGCACCGGGTAAGTTGATTAAGCTTTCCACTGCGGTGGTTCTGGATGAATCGGTGAAAA
ATCTGGATCGAACACAGGTTGAAAAGATTGTAACCGCAGCCATTGGTTTTGATGATCAACGGGGTGATCAGATTACGGTA
TCTGCCATGACCTTTGATCGAAGCCAATACGACTTAGACCAGGATTTGCCTGATAACAGCCAAAAGGAAAAAGAACTATT
TTATATGACCATTGCAGCGGTTGTGGGAGGCTTACTGCTTCTGTTGCTTCTGGTGGCTTATTGGCTACGTCGGCGTAGAA
AGAACGCAGAACAAGAATTGCAGGTTATTGAAGAAACTACGCCAATGCCAATTGAGAGTGTGACTACTAAGGAAGAAGAG
GAAGAAGAGGAAGAAGCCCAGTGGGAAATTCCTCCGCCCAAGGATAAACAAAAGCAGTTAAAGGAACTGGCCCAAGAACG
ACCCGATGATATTGCCTCGGTACTGAAAGTATGGCTTAGGGAATAG

Upstream 100 bases:

>100_bases
AAACTGACCATGCAACTGGCGGTGGAAGTAAGAAATAAAATTGTTGAAGCTTATCAAGAAATATCCAGGATACAATTGTA
ATTTTCGTTGAGGTGGTTAT

Downstream 100 bases:

>100_bases
GTGATAAAAGTCATGAATCAATATGAAAAATTAACCGGAGAACAAAAGGCGGCTATTTTACTTATCTCCCTGGGAGCTGA
TGTTTCCTCAAAGCTATTAA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MNFNDLLERLKQRWQETSQNRKVLVILVSTVVLAGLVYLAMTLTKPNYAILLGDLEPKDAGDIVAQLESEKIPYQLIDQG
TTILVPQEQVDEIRVKLASDGLLTGVGNGFELFDKSKFGETDFEQQISYQRALQEELRRTITKVEGVEQARVHLVLPRKS
VFIEEEGTASASVVVKLKPRAKLKAEQVKGLNDLIIGSVEGLQAENVHIIDTEGNVLNDFLKDSSGTPGNGFSGTILERQ
QQIRRTYEKELENRVQNMLAKVLGPNKAVAMVTAEMDFNQLQTNTTEVVPGQVVSERSIKEQGSDGSGGGVTGSTTQMPG
QSIPAETSTSQNSYIKTDDTKNYQHGTRTQSEVQAPGKLIKLSTAVVLDESVKNLDRTQVEKIVTAAIGFDDQRGDQITV
SAMTFDRSQYDLDQDLPDNSQKEKELFYMTIAAVVGGLLLLLLLVAYWLRRRRKNAEQELQVIEETTPMPIESVTTKEEE
EEEEEAQWEIPPPKDKQKQLKELAQERPDDIASVLKVWLRE

Sequences:

>Translated_521_residues
MNFNDLLERLKQRWQETSQNRKVLVILVSTVVLAGLVYLAMTLTKPNYAILLGDLEPKDAGDIVAQLESEKIPYQLIDQG
TTILVPQEQVDEIRVKLASDGLLTGVGNGFELFDKSKFGETDFEQQISYQRALQEELRRTITKVEGVEQARVHLVLPRKS
VFIEEEGTASASVVVKLKPRAKLKAEQVKGLNDLIIGSVEGLQAENVHIIDTEGNVLNDFLKDSSGTPGNGFSGTILERQ
QQIRRTYEKELENRVQNMLAKVLGPNKAVAMVTAEMDFNQLQTNTTEVVPGQVVSERSIKEQGSDGSGGGVTGSTTQMPG
QSIPAETSTSQNSYIKTDDTKNYQHGTRTQSEVQAPGKLIKLSTAVVLDESVKNLDRTQVEKIVTAAIGFDDQRGDQITV
SAMTFDRSQYDLDQDLPDNSQKEKELFYMTIAAVVGGLLLLLLLVAYWLRRRRKNAEQELQVIEETTPMPIESVTTKEEE
EEEEEAQWEIPPPKDKQKQLKELAQERPDDIASVLKVWLRE
>Mature_521_residues
MNFNDLLERLKQRWQETSQNRKVLVILVSTVVLAGLVYLAMTLTKPNYAILLGDLEPKDAGDIVAQLESEKIPYQLIDQG
TTILVPQEQVDEIRVKLASDGLLTGVGNGFELFDKSKFGETDFEQQISYQRALQEELRRTITKVEGVEQARVHLVLPRKS
VFIEEEGTASASVVVKLKPRAKLKAEQVKGLNDLIIGSVEGLQAENVHIIDTEGNVLNDFLKDSSGTPGNGFSGTILERQ
QQIRRTYEKELENRVQNMLAKVLGPNKAVAMVTAEMDFNQLQTNTTEVVPGQVVSERSIKEQGSDGSGGGVTGSTTQMPG
QSIPAETSTSQNSYIKTDDTKNYQHGTRTQSEVQAPGKLIKLSTAVVLDESVKNLDRTQVEKIVTAAIGFDDQRGDQITV
SAMTFDRSQYDLDQDLPDNSQKEKELFYMTIAAVVGGLLLLLLLVAYWLRRRRKNAEQELQVIEETTPMPIESVTTKEEE
EEEEEAQWEIPPPKDKQKQLKELAQERPDDIASVLKVWLRE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=422, Percent_Identity=30.8056872037915, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 58171; Mature: 58171

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNDLLERLKQRWQETSQNRKVLVILVSTVVLAGLVYLAMTLTKPNYAILLGDLEPKDA
CCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
GDIVAQLESEKIPYQLIDQGTTILVPQEQVDEIRVKLASDGLLTGVGNGFELFDKSKFGE
HHHHHHHHCCCCCHHHHCCCCEEEECHHHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCC
TDFEQQISYQRALQEELRRTITKVEGVEQARVHLVLPRKSVFIEEEGTASASVVVKLKPR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEECCC
AKLKAEQVKGLNDLIIGSVEGLQAENVHIIDTEGNVLNDFLKDSSGTPGNGFSGTILERQ
HHHHHHHHCCCHHHHEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHH
QQIRRTYEKELENRVQNMLAKVLGPNKAVAMVTAEMDFNQLQTNTTEVVPGQVVSERSIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCCCCCHHHHHHHHH
EQGSDGSGGGVTGSTTQMPGQSIPAETSTSQNSYIKTDDTKNYQHGTRTQSEVQAPGKLI
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCEE
KLSTAVVLDESVKNLDRTQVEKIVTAAIGFDDQRGDQITVSAMTFDRSQYDLDQDLPDNS
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCC
QKEKELFYMTIAAVVGGLLLLLLLVAYWLRRRRKNAEQELQVIEETTPMPIESVTTKEEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCHHH
EEEEEAQWEIPPPKDKQKQLKELAQERPDDIASVLKVWLRE
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNFNDLLERLKQRWQETSQNRKVLVILVSTVVLAGLVYLAMTLTKPNYAILLGDLEPKDA
CCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
GDIVAQLESEKIPYQLIDQGTTILVPQEQVDEIRVKLASDGLLTGVGNGFELFDKSKFGE
HHHHHHHHCCCCCHHHHCCCCEEEECHHHHHHHHHHHHCCCCEECCCCCHHHHCCCCCCC
TDFEQQISYQRALQEELRRTITKVEGVEQARVHLVLPRKSVFIEEEGTASASVVVKLKPR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCEEEEEEECCC
AKLKAEQVKGLNDLIIGSVEGLQAENVHIIDTEGNVLNDFLKDSSGTPGNGFSGTILERQ
HHHHHHHHCCCHHHHEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHH
QQIRRTYEKELENRVQNMLAKVLGPNKAVAMVTAEMDFNQLQTNTTEVVPGQVVSERSIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHCCCCCCCCCHHHHHHHHH
EQGSDGSGGGVTGSTTQMPGQSIPAETSTSQNSYIKTDDTKNYQHGTRTQSEVQAPGKLI
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHCCCCEE
KLSTAVVLDESVKNLDRTQVEKIVTAAIGFDDQRGDQITVSAMTFDRSQYDLDQDLPDNS
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCC
QKEKELFYMTIAAVVGGLLLLLLLVAYWLRRRRKNAEQELQVIEETTPMPIESVTTKEEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHCCCHHH
EEEEEAQWEIPPPKDKQKQLKELAQERPDDIASVLKVWLRE
HHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]