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The map label for this gene is purN [H]

Identifier: 134300202

GI number: 134300202

Start: 2587203

End: 2587814

Strand: Reverse

Name: purN [H]

Synonym: Dred_2363

Alternate gene names: 134300202

Gene position: 2587814-2587203 (Counterclockwise)

Preceding gene: 134300203

Following gene: 134300201

Centisome position: 71.72

GC content: 50.98

Gene sequence:

>612_bases
ATGAATAAATTACGGATAGGGGTATTGGCTTCGGGGCGGGGTTCCAATTTGCAGTCCATCCTGGATCGCTGCCAGGAGGG
GACAGTTGCGGCGGAAGTGGTGGTGGTAATCAGCGATAAACCTGCTGCCTATGCCCTGGAAAGGGCCCGGCAGGCAGGGA
TTACGGCCTTTGGGTTAGAGATTAGATCCTTTCCCGGTAAGAGAGAATACGAACAGGCTGTTGTTAAGCTTTTGCAAGAT
GCTGGTGTGGAACTGGTTTGCCTGGCAGGCTACATGCGCTTGGTGGGGGAATCCCTGTTAAGGGCTTTCCCTAATCGGAT
TATGAACATTCATCCTGCTTTATTGCCATCCTTTACCGGCTTACATGGGCAGAGGGATGCCCTGCAGTATGGGGTTAAGA
TATCTGGTTGCACGGTGCATTTTGTGGATGAAGGGATGGACACCGGCCCTATTATTTTACAAGCAGCGGTTCCGGTGCTG
GATGACGATACAGAGGAAAGCCTGTCAGCCCGGATTCTCAATCAGGAACACCGGATTTATCCAGAGGCAGTTAAGTTGTT
TGCTGAAGGACGGCTGCAGGTTGTGGGGCGGAAAGTATATATTAAGAAATGA

Upstream 100 bases:

>100_bases
CAGCCGTCATGAACAGGCCTATCTGATTGGACAGGTGGAAAGTGGTCCTTCACAAGTTGAATATATACAGGGTTAAAGAT
CAGCCTTAGGGAGAAATCTT

Downstream 100 bases:

>100_bases
AGGGCAGATCGGTAATGATGGTTTGCCCATAACCCATTAAGTGAAAGGCGTGGTAAGGAAATGAAAATCAAACGCGCTTT
AATTAGTGTTTCCGATAAAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MNKLRIGVLASGRGSNLQSILDRCQEGTVAAEVVVVISDKPAAYALERARQAGITAFGLEIRSFPGKREYEQAVVKLLQD
AGVELVCLAGYMRLVGESLLRAFPNRIMNIHPALLPSFTGLHGQRDALQYGVKISGCTVHFVDEGMDTGPIILQAAVPVL
DDDTEESLSARILNQEHRIYPEAVKLFAEGRLQVVGRKVYIKK

Sequences:

>Translated_203_residues
MNKLRIGVLASGRGSNLQSILDRCQEGTVAAEVVVVISDKPAAYALERARQAGITAFGLEIRSFPGKREYEQAVVKLLQD
AGVELVCLAGYMRLVGESLLRAFPNRIMNIHPALLPSFTGLHGQRDALQYGVKISGCTVHFVDEGMDTGPIILQAAVPVL
DDDTEESLSARILNQEHRIYPEAVKLFAEGRLQVVGRKVYIKK
>Mature_203_residues
MNKLRIGVLASGRGSNLQSILDRCQEGTVAAEVVVVISDKPAAYALERARQAGITAFGLEIRSFPGKREYEQAVVKLLQD
AGVELVCLAGYMRLVGESLLRAFPNRIMNIHPALLPSFTGLHGQRDALQYGVKISGCTVHFVDEGMDTGPIILQAAVPVL
DDDTEESLSARILNQEHRIYPEAVKLFAEGRLQVVGRKVYIKK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=204, Percent_Identity=40.1960784313725, Blast_Score=168, Evalue=3e-42,
Organism=Homo sapiens, GI209869995, Length=204, Percent_Identity=40.1960784313725, Blast_Score=168, Evalue=3e-42,
Organism=Homo sapiens, GI209869993, Length=204, Percent_Identity=40.1960784313725, Blast_Score=168, Evalue=3e-42,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=42.4083769633508, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787483, Length=200, Percent_Identity=29, Blast_Score=82, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=185, Percent_Identity=40.5405405405405, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=27.1356783919598, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=46.8085106382979, Blast_Score=181, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22214; Mature: 22214

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLRIGVLASGRGSNLQSILDRCQEGTVAAEVVVVISDKPAAYALERARQAGITAFGLE
CCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEE
IRSFPGKREYEQAVVKLLQDAGVELVCLAGYMRLVGESLLRAFPNRIMNIHPALLPSFTG
EECCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
LHGQRDALQYGVKISGCTVHFVDEGMDTGPIILQAAVPVLDDDTEESLSARILNQEHRIY
CCCCHHHHHHCCEEECEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCC
PEAVKLFAEGRLQVVGRKVYIKK
HHHHHHHHCCCEEEEEEEEEEEC
>Mature Secondary Structure
MNKLRIGVLASGRGSNLQSILDRCQEGTVAAEVVVVISDKPAAYALERARQAGITAFGLE
CCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEE
IRSFPGKREYEQAVVKLLQDAGVELVCLAGYMRLVGESLLRAFPNRIMNIHPALLPSFTG
EECCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
LHGQRDALQYGVKISGCTVHFVDEGMDTGPIILQAAVPVLDDDTEESLSARILNQEHRIY
CCCCHHHHHHCCEEECEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCC
PEAVKLFAEGRLQVVGRKVYIKK
HHHHHHHHCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]