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The map label for this gene is hisZ

Identifier: 134300198

GI number: 134300198

Start: 2582506

End: 2583711

Strand: Reverse

Name: hisZ

Synonym: Dred_2359

Alternate gene names: 134300198

Gene position: 2583711-2582506 (Counterclockwise)

Preceding gene: 134300199

Following gene: 134300196

Centisome position: 71.61

GC content: 48.51

Gene sequence:

>1206_bases
ATGACATCAAGCAGATTTGGTCGGCTGGCTCCCGGTGTCAGGGATGTACTGCCCGCAGAAGCTCATTTAATGCGTGGGCT
AAAAGAAAAATTCACCAAACTGGTGGAGACCTGGGGGTATAAAGAAGTTGTAACCCCCACCTTTGAATACATGGAAAATC
TGGCCTCTGAGGAACTACAGGAAGAAAAGTTTTTTAAGTTCCTGGACCGGCAGGGCCATTTAATGGCTCTACGCCCGGAT
ATGACACGTCCAATGGCTCGTTTGGTGGCCACCCGTATGAAAGGGATCACACCACCCCTAAGACTTTTTTACTTGGCCAA
TGTTTTCAATTATGAACAGCCCCAGGTAGGTCGCCAAAGGGAGTTTTATCAGGCAGGGGTGGAATTAATGGGCCCTTCGT
CGCCTGAAGCTGATGCTGAAGTGGTGGCAATGGTGGCTGAATATTTAATGCAGACCGGATTAGCCGATTTTCAAATTAGC
ATTGGTAATGTAGGCATATTTCATGGCTTAGTAAAGCAACTGGGTCTGCCCAAGGAAGTAGCCCAGGAACTGAAAGAAGC
CCTGGGAAATAAAGATTTTGTTAAGGTGGAAGAAATTCTTGGCTCCCACGCGGCCACACCGGAGGAAGCTCGCCGGACAC
TGGATCTTATTGAATTGCGTGGCGGGCCGGAAATATTAGACCGGGCATTTGGGTTGGCCCAACCAGGGCCAGCTGCTGAT
GCCATTGATAATCTTCGTCAACTGTACTGGGGATTAACCTGTTATGGCGTGGAAAGGCATATAACCCTGGATTTAGGTTT
GTTAAGGGGACTGGATTATTATACTGGTCTGGTATTTGAAGGTTATACCGTAGCCATGGGCTTTCCCATTTGTGGTGGAG
GTCGCTACAATCAACTTTTGGCAAAATTTGGTTTACCCATGCCTGCCACGGGCTTTGCGGTTAATCTAGAACGGGTTTTG
GTGGCACTGGAGCGTCTACAGGGTCCCCCAAGGGAACCAGTACCGGATGTACTGGTGGCTTGGGAGGATAATAGTCTGGC
TAATGCCCTCCAATGTGTAAAGGAATTAAGATACCAGGGACTAAAGGTGGTTACTGCCATGTTCGAATATCCACCGGCAA
AGGCCAAGGCCGAGGCCAGAAGCCTGGGAGCTTCTAGGGTGATCTATTTTGATAAAGAAGGCAAAAGTGAGGAGTTAAGC
CTATAA

Upstream 100 bases:

>100_bases
TAAACTATGGGGCGGATGGTTATAAGCTAGTTTTAAATCGTTTAGCTGATCCAACTCCATTAAAAGAACAAAAAACAGAA
GAATAAAAAGAGGTGAATTT

Downstream 100 bases:

>100_bases
TCTGTTTATTTTTGATAAAAGATACCCCTTCGGAAAGCTAATGTTTTAGCACTTTCCAGAGGGGTATCTTTTATCTTCAT
CTCTGTTTCCAGACAGGTTA

Product: histidine--tRNA ligase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTSSRFGRLAPGVRDVLPAEAHLMRGLKEKFTKLVETWGYKEVVTPTFEYMENLASEELQEEKFFKFLDRQGHLMALRPD
MTRPMARLVATRMKGITPPLRLFYLANVFNYEQPQVGRQREFYQAGVELMGPSSPEADAEVVAMVAEYLMQTGLADFQIS
IGNVGIFHGLVKQLGLPKEVAQELKEALGNKDFVKVEEILGSHAATPEEARRTLDLIELRGGPEILDRAFGLAQPGPAAD
AIDNLRQLYWGLTCYGVERHITLDLGLLRGLDYYTGLVFEGYTVAMGFPICGGGRYNQLLAKFGLPMPATGFAVNLERVL
VALERLQGPPREPVPDVLVAWEDNSLANALQCVKELRYQGLKVVTAMFEYPPAKAKAEARSLGASRVIYFDKEGKSEELS
L

Sequences:

>Translated_401_residues
MTSSRFGRLAPGVRDVLPAEAHLMRGLKEKFTKLVETWGYKEVVTPTFEYMENLASEELQEEKFFKFLDRQGHLMALRPD
MTRPMARLVATRMKGITPPLRLFYLANVFNYEQPQVGRQREFYQAGVELMGPSSPEADAEVVAMVAEYLMQTGLADFQIS
IGNVGIFHGLVKQLGLPKEVAQELKEALGNKDFVKVEEILGSHAATPEEARRTLDLIELRGGPEILDRAFGLAQPGPAAD
AIDNLRQLYWGLTCYGVERHITLDLGLLRGLDYYTGLVFEGYTVAMGFPICGGGRYNQLLAKFGLPMPATGFAVNLERVL
VALERLQGPPREPVPDVLVAWEDNSLANALQCVKELRYQGLKVVTAMFEYPPAKAKAEARSLGASRVIYFDKEGKSEELS
L
>Mature_400_residues
TSSRFGRLAPGVRDVLPAEAHLMRGLKEKFTKLVETWGYKEVVTPTFEYMENLASEELQEEKFFKFLDRQGHLMALRPDM
TRPMARLVATRMKGITPPLRLFYLANVFNYEQPQVGRQREFYQAGVELMGPSSPEADAEVVAMVAEYLMQTGLADFQISI
GNVGIFHGLVKQLGLPKEVAQELKEALGNKDFVKVEEILGSHAATPEEARRTLDLIELRGGPEILDRAFGLAQPGPAADA
IDNLRQLYWGLTCYGVERHITLDLGLLRGLDYYTGLVFEGYTVAMGFPICGGGRYNQLLAKFGLPMPATGFAVNLERVLV
ALERLQGPPREPVPDVLVAWEDNSLANALQCVKELRYQGLKVVTAMFEYPPAKAKAEARSLGASRVIYFDKEGKSEELSL

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=380, Percent_Identity=28.1578947368421, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI15029520, Length=387, Percent_Identity=28.6821705426357, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1788861, Length=375, Percent_Identity=26.1333333333333, Blast_Score=111, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71993693, Length=407, Percent_Identity=26.5356265356265, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=407, Percent_Identity=26.5356265356265, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325290, Length=357, Percent_Identity=25.4901960784314, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24643061, Length=361, Percent_Identity=26.5927977839335, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24643059, Length=380, Percent_Identity=25.2631578947368, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_DESRM (A4J716)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113694.1
- STRING:   A4J716
- GeneID:   4955955
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2359
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   YREHVKS
- PhylomeDB:   A4J716
- ProtClustDB:   CLSK968269
- BioCyc:   DRED349161:DRED_2359-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 44665; Mature: 44534

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSRFGRLAPGVRDVLPAEAHLMRGLKEKFTKLVETWGYKEVVTPTFEYMENLASEELQ
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHH
EEKFFKFLDRQGHLMALRPDMTRPMARLVATRMKGITPPLRLFYLANVFNYEQPQVGRQR
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHH
EFYQAGVELMGPSSPEADAEVVAMVAEYLMQTGLADFQISIGNVGIFHGLVKQLGLPKEV
HHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCHHH
AQELKEALGNKDFVKVEEILGSHAATPEEARRTLDLIELRGGPEILDRAFGLAQPGPAAD
HHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHH
AIDNLRQLYWGLTCYGVERHITLDLGLLRGLDYYTGLVFEGYTVAMGFPICGGGRYNQLL
HHHHHHHHHHHHHEEECCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
AKFGLPMPATGFAVNLERVLVALERLQGPPREPVPDVLVAWEDNSLANALQCVKELRYQG
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
LKVVTAMFEYPPAKAKAEARSLGASRVIYFDKEGKSEELSL
HHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TSSRFGRLAPGVRDVLPAEAHLMRGLKEKFTKLVETWGYKEVVTPTFEYMENLASEELQ
CCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHH
EEKFFKFLDRQGHLMALRPDMTRPMARLVATRMKGITPPLRLFYLANVFNYEQPQVGRQR
HHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHH
EFYQAGVELMGPSSPEADAEVVAMVAEYLMQTGLADFQISIGNVGIFHGLVKQLGLPKEV
HHHHHCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCHHH
AQELKEALGNKDFVKVEEILGSHAATPEEARRTLDLIELRGGPEILDRAFGLAQPGPAAD
HHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHH
AIDNLRQLYWGLTCYGVERHITLDLGLLRGLDYYTGLVFEGYTVAMGFPICGGGRYNQLL
HHHHHHHHHHHHHEEECCEEEEEHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
AKFGLPMPATGFAVNLERVLVALERLQGPPREPVPDVLVAWEDNSLANALQCVKELRYQG
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
LKVVTAMFEYPPAKAKAEARSLGASRVIYFDKEGKSEELSL
HHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA