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The map label for this gene is gatA

Identifier: 134300180

GI number: 134300180

Start: 2559819

End: 2561276

Strand: Reverse

Name: gatA

Synonym: Dred_2341

Alternate gene names: 134300180

Gene position: 2561276-2559819 (Counterclockwise)

Preceding gene: 134300181

Following gene: 134300179

Centisome position: 70.99

GC content: 49.18

Gene sequence:

>1458_bases
TTGGATCTCTTTCAGCTTACTGCCCACCAGCTTCATCAACTGCTTACGAAAAAAGAAATCTCTGCGCTGGATATAACCGA
AGCTGTTTACAACCGTATTGAGCAGGTTGAGGATAAAGTTAAGTCCTACCTTACGCTTACCAGGGAACAGGCCGAAATAA
AGGCCCGACAGGTGGACCAAAAAATTGCCGCCGGAGAGTCCATTGGACCCTTGGCGGGTATTCCCATTGCCATTAAGGAC
AACATGTGCACCCAGGGGATTCGTACCACCTGTGCATCTAAGATCCTTTACAACTTTGTTCCCCCATACTCCGCCACCTC
AGTGGAAAAGGTTTATACTGCGGATATGGTTATGGTGGGAAAAACCAACATGGATGAATTTGCCATGGGTTCTTCCACGG
AAAACTCGGGCTTTCATTTAACCCACAACCCCTGGGATTTGGACCGAGTTCCCGGCGGGTCCAGTGGTGGATCAGCTGCA
GCAGTGGCTGCCGGGGAAGCGATTATTTCCTTGGGTTCTGACACCGGTGGTTCCATTCGCCAGCCTGCTGCCCTTTGCGG
TGTAGTAGGAATGAAACCAACCTACGGTAGTGTATCTCGCTATGGGCTGGTGGCTTATGCGTCCTCTCTGGATCAAATTG
GACCTTTCACTCGGGATGTTACAGACATGGCCCATGTCCTTAACGTGATCTGCGGCCATGACCCGATGGACAGTACTTCG
GCCAATTTAAAACAGACAGACTATACCCAGTTTCTTACCAACGATATTAAGGGTATGAAAATTGGTGTGCCCCGGGAATA
TATGGCGGACGGCATTGACCCGCAGGTTAGGGAAAAAATCAAAGCTGCCATCCAAAAACTTACAGAGCTGGGCGCTCATG
TTGAAGAAACCAGCATGCCCCATACGGATTATGCCATGCCAGCTTATTATCTAATTGCTACAGCCGAGGCAAGCTCCAAC
CTAGCCCGCTATGACGGAGTACGCTACGGCCTGAGGGTTGAAGAGGCCCGTGATTTAGTGGATATGTTCATGCGCAGCCG
CAGCCAGGGCTTTGGTGATGAAGTGAAGCGCCGCATTATGTTGGGAACCTATTCCCTGTCTGCGGGCTACTACGATGCTT
ACTATCTAAAGGCTCTAAAGGTGCGAACCCTAATAAAACAAGACTTTGATCGTGCCTTTGAAAAATATGATGCACTGTTA
TCACCCACTTCTCCCACAACTGCCTTTAAAATTGGAGAAATGGTCAATGACCCCATCCAGATGTATTTACAGGATGTTTG
CACCATACCGGTGAACTTGGCAGGCATTCCGGCAATCTCTCTGCCCTGCGGTCTGGCCAATAACCTGCCTGTGGGTCTGC
AGCTAATGGGCAAAGCCTTTGATGAAGGAACTCTTTTGAGAATTGCCTATACCTTAGAGCAAAATACGGAATATACAAGG
CTTCGGCCTGAGATATAG

Upstream 100 bases:

>100_bases
AAAGCATATTGACCCGGAAAAAGCCCTAGCCAATGCTCCGGACCGGGAAGAAAACTTTTTTAAAGTGCCGAAAATTGTTT
AGTAAATAGGAGGGAGCATT

Downstream 100 bases:

>100_bases
GGGGTGGAGCAGATGACACAACAGTATGAAACTGTCATCGGTATTGAAGTACACGTTGAACTGAAAACCAACACAAAGAT
TTTTTGTAACTCCACCACCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MDLFQLTAHQLHQLLTKKEISALDITEAVYNRIEQVEDKVKSYLTLTREQAEIKARQVDQKIAAGESIGPLAGIPIAIKD
NMCTQGIRTTCASKILYNFVPPYSATSVEKVYTADMVMVGKTNMDEFAMGSSTENSGFHLTHNPWDLDRVPGGSSGGSAA
AVAAGEAIISLGSDTGGSIRQPAALCGVVGMKPTYGSVSRYGLVAYASSLDQIGPFTRDVTDMAHVLNVICGHDPMDSTS
ANLKQTDYTQFLTNDIKGMKIGVPREYMADGIDPQVREKIKAAIQKLTELGAHVEETSMPHTDYAMPAYYLIATAEASSN
LARYDGVRYGLRVEEARDLVDMFMRSRSQGFGDEVKRRIMLGTYSLSAGYYDAYYLKALKVRTLIKQDFDRAFEKYDALL
SPTSPTTAFKIGEMVNDPIQMYLQDVCTIPVNLAGIPAISLPCGLANNLPVGLQLMGKAFDEGTLLRIAYTLEQNTEYTR
LRPEI

Sequences:

>Translated_485_residues
MDLFQLTAHQLHQLLTKKEISALDITEAVYNRIEQVEDKVKSYLTLTREQAEIKARQVDQKIAAGESIGPLAGIPIAIKD
NMCTQGIRTTCASKILYNFVPPYSATSVEKVYTADMVMVGKTNMDEFAMGSSTENSGFHLTHNPWDLDRVPGGSSGGSAA
AVAAGEAIISLGSDTGGSIRQPAALCGVVGMKPTYGSVSRYGLVAYASSLDQIGPFTRDVTDMAHVLNVICGHDPMDSTS
ANLKQTDYTQFLTNDIKGMKIGVPREYMADGIDPQVREKIKAAIQKLTELGAHVEETSMPHTDYAMPAYYLIATAEASSN
LARYDGVRYGLRVEEARDLVDMFMRSRSQGFGDEVKRRIMLGTYSLSAGYYDAYYLKALKVRTLIKQDFDRAFEKYDALL
SPTSPTTAFKIGEMVNDPIQMYLQDVCTIPVNLAGIPAISLPCGLANNLPVGLQLMGKAFDEGTLLRIAYTLEQNTEYTR
LRPEI
>Mature_485_residues
MDLFQLTAHQLHQLLTKKEISALDITEAVYNRIEQVEDKVKSYLTLTREQAEIKARQVDQKIAAGESIGPLAGIPIAIKD
NMCTQGIRTTCASKILYNFVPPYSATSVEKVYTADMVMVGKTNMDEFAMGSSTENSGFHLTHNPWDLDRVPGGSSGGSAA
AVAAGEAIISLGSDTGGSIRQPAALCGVVGMKPTYGSVSRYGLVAYASSLDQIGPFTRDVTDMAHVLNVICGHDPMDSTS
ANLKQTDYTQFLTNDIKGMKIGVPREYMADGIDPQVREKIKAAIQKLTELGAHVEETSMPHTDYAMPAYYLIATAEASSN
LARYDGVRYGLRVEEARDLVDMFMRSRSQGFGDEVKRRIMLGTYSLSAGYYDAYYLKALKVRTLIKQDFDRAFEKYDALL
SPTSPTTAFKIGEMVNDPIQMYLQDVCTIPVNLAGIPAISLPCGLANNLPVGLQLMGKAFDEGTLLRIAYTLEQNTEYTR
LRPEI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=490, Percent_Identity=36.530612244898, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI195972892, Length=501, Percent_Identity=25.1497005988024, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17543272, Length=455, Percent_Identity=36.9230769230769, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=26.369168356998, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=300, Percent_Identity=28, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=315, Percent_Identity=24.7619047619048, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=316, Percent_Identity=24.0506329113924, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=324, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=324, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=36.1244019138756, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=29.0909090909091, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=231, Percent_Identity=29.004329004329, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648113, Length=459, Percent_Identity=42.2657952069717, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24652985, Length=488, Percent_Identity=23.7704918032787, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI19922090, Length=488, Percent_Identity=23.7704918032787, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24652981, Length=488, Percent_Identity=23.7704918032787, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24652983, Length=488, Percent_Identity=23.7704918032787, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45550774, Length=249, Percent_Identity=32.5301204819277, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648435, Length=249, Percent_Identity=32.5301204819277, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648437, Length=249, Percent_Identity=32.5301204819277, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24644968, Length=507, Percent_Identity=24.4575936883629, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078093, Length=507, Percent_Identity=25.2465483234714, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356731, Length=499, Percent_Identity=25.250501002004, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648441, Length=179, Percent_Identity=35.195530726257, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648439, Length=179, Percent_Identity=35.195530726257, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_DESRM (A4J6Z8)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113676.1
- ProteinModelPortal:   A4J6Z8
- SMR:   A4J6Z8
- STRING:   A4J6Z8
- GeneID:   4957023
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2341
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   A4J6Z8
- ProtClustDB:   PRK00012
- BioCyc:   DRED349161:DRED_2341-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53137; Mature: 53137

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFQLTAHQLHQLLTKKEISALDITEAVYNRIEQVEDKVKSYLTLTREQAEIKARQVDQ
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIAAGESIGPLAGIPIAIKDNMCTQGIRTTCASKILYNFVPPYSATSVEKVYTADMVMVG
HHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHECCEEEEC
KTNMDEFAMGSSTENSGFHLTHNPWDLDRVPGGSSGGSAAAVAAGEAIISLGSDTGGSIR
CCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCHHHEEECCCCCCCCC
QPAALCGVVGMKPTYGSVSRYGLVAYASSLDQIGPFTRDVTDMAHVLNVICGHDPMDSTS
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
ANLKQTDYTQFLTNDIKGMKIGVPREYMADGIDPQVREKIKAAIQKLTELGAHVEETSMP
CCCCHHHHHHHHHHCCCCCEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCC
HTDYAMPAYYLIATAEASSNLARYDGVRYGLRVEEARDLVDMFMRSRSQGFGDEVKRRIM
CCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LGTYSLSAGYYDAYYLKALKVRTLIKQDFDRAFEKYDALLSPTSPTTAFKIGEMVNDPIQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHH
MYLQDVCTIPVNLAGIPAISLPCGLANNLPVGLQLMGKAFDEGTLLRIAYTLEQNTEYTR
HHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
LRPEI
CCCCC
>Mature Secondary Structure
MDLFQLTAHQLHQLLTKKEISALDITEAVYNRIEQVEDKVKSYLTLTREQAEIKARQVDQ
CCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIAAGESIGPLAGIPIAIKDNMCTQGIRTTCASKILYNFVPPYSATSVEKVYTADMVMVG
HHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHECCEEEEC
KTNMDEFAMGSSTENSGFHLTHNPWDLDRVPGGSSGGSAAAVAAGEAIISLGSDTGGSIR
CCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHCHHHEEECCCCCCCCC
QPAALCGVVGMKPTYGSVSRYGLVAYASSLDQIGPFTRDVTDMAHVLNVICGHDPMDSTS
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
ANLKQTDYTQFLTNDIKGMKIGVPREYMADGIDPQVREKIKAAIQKLTELGAHVEETSMP
CCCCHHHHHHHHHHCCCCCEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCC
HTDYAMPAYYLIATAEASSNLARYDGVRYGLRVEEARDLVDMFMRSRSQGFGDEVKRRIM
CCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LGTYSLSAGYYDAYYLKALKVRTLIKQDFDRAFEKYDALLSPTSPTTAFKIGEMVNDPIQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHH
MYLQDVCTIPVNLAGIPAISLPCGLANNLPVGLQLMGKAFDEGTLLRIAYTLEQNTEYTR
HHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
LRPEI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA