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The map label for this gene is pfkA [H]

Identifier: 134300152

GI number: 134300152

Start: 2531081

End: 2532043

Strand: Reverse

Name: pfkA [H]

Synonym: Dred_2313

Alternate gene names: 134300152

Gene position: 2532043-2531081 (Counterclockwise)

Preceding gene: 134300153

Following gene: 134300151

Centisome position: 70.18

GC content: 45.17

Gene sequence:

>963_bases
ATGCAGAGAATAGGCCTATTAACCAGCGGCGGGGATGCTTCGGGCATGAACTCCTCGATACGTGCGGTGGTTCGTAAAGC
CATTTACCATGGCCTGGAGGTTATTGGTATCCGGCGTGGCTATAGCGGGTTTATTGAGGCTGATATGGGTCCCATGAATC
TTGGCTCAGTTGCAGATATTATTGGTCGAGGGGGAACCATTTTACATACTGCCCGATCCGAAAGGTTTAAAACCAAAGAA
GGCCGAGCCTTGGCAATGGAAAACATACAACGCTTTGGTATCCAAGGCTTGGTGGTTATTGGTGGGGATGGTTCCTTTAC
AGGGGCCAGTATTTTGCACCAGGAATATGGCATACCAATGATTGGTGTACCCGGAACCATTGATAATGATATTGCTGGGA
CGGATTATACCATTGGTTTTGATACAGCTGTTAACACCGTGGTGGATGCCATTAATAAAATTAGAGATACTGCAACTTCC
CATGAACGGACCTTTGTCATTGAAGTAATGGGGCGAAACTCGGGTAATATTGCCCTAGCGGCCGGACTGGCCGGTGGGGC
GGAAAGTATATTAATTCCGGAGATACCTTTTAATATTGAAGAGATTTGTCAAAAACTTTTAAGGGGCATTAAGAGAGGTA
AACTTCACTCAATCATTCTAGTAGCCGAAGGTGCTGCCAGTGGAATTGATATTGGTCGACAAGTCAAAGAACAAATTGGC
TTTGATACCAAAGTAACTATCCTTGGACATTTACAGAGGGGTGGTACGCCTACCGCCTTTGATCGTATTCTGGCGGCTAG
ATTGGGTGCAAAGGCGGTGGAATTGTTGATGGCGGGAGAAACCAATAAAATGGTTGGCATTAAAGCCGGTGAAATTGTGG
CCACAGATTTAACCGAAGTCATTGGAAAACCCAGCCCCATTAATAAGAACATGTACGAATTAGCAAAGATATTATCCATA
TAG

Upstream 100 bases:

>100_bases
GGGGCAAACTGGTTAAATAACATTTATATTCAAAGGATAAGCCTGCCGTTTTGCGGCAGGCTATAGGTGTATCTTAGGGA
CTTAAAGGAGGCTTTTTTCA

Downstream 100 bases:

>100_bases
AAAAAACAGTAACTATATTTTTGTTTTTAGCAAGGGAGGTTCTGTGGATGAGACGTACAAAGATTGTTTGCACCATCGGT
CCCGCCAGTGAAGATGTGGA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MQRIGLLTSGGDASGMNSSIRAVVRKAIYHGLEVIGIRRGYSGFIEADMGPMNLGSVADIIGRGGTILHTARSERFKTKE
GRALAMENIQRFGIQGLVVIGGDGSFTGASILHQEYGIPMIGVPGTIDNDIAGTDYTIGFDTAVNTVVDAINKIRDTATS
HERTFVIEVMGRNSGNIALAAGLAGGAESILIPEIPFNIEEICQKLLRGIKRGKLHSIILVAEGAASGIDIGRQVKEQIG
FDTKVTILGHLQRGGTPTAFDRILAARLGAKAVELLMAGETNKMVGIKAGEIVATDLTEVIGKPSPINKNMYELAKILSI

Sequences:

>Translated_320_residues
MQRIGLLTSGGDASGMNSSIRAVVRKAIYHGLEVIGIRRGYSGFIEADMGPMNLGSVADIIGRGGTILHTARSERFKTKE
GRALAMENIQRFGIQGLVVIGGDGSFTGASILHQEYGIPMIGVPGTIDNDIAGTDYTIGFDTAVNTVVDAINKIRDTATS
HERTFVIEVMGRNSGNIALAAGLAGGAESILIPEIPFNIEEICQKLLRGIKRGKLHSIILVAEGAASGIDIGRQVKEQIG
FDTKVTILGHLQRGGTPTAFDRILAARLGAKAVELLMAGETNKMVGIKAGEIVATDLTEVIGKPSPINKNMYELAKILSI
>Mature_320_residues
MQRIGLLTSGGDASGMNSSIRAVVRKAIYHGLEVIGIRRGYSGFIEADMGPMNLGSVADIIGRGGTILHTARSERFKTKE
GRALAMENIQRFGIQGLVVIGGDGSFTGASILHQEYGIPMIGVPGTIDNDIAGTDYTIGFDTAVNTVVDAINKIRDTATS
HERTFVIEVMGRNSGNIALAAGLAGGAESILIPEIPFNIEEICQKLLRGIKRGKLHSIILVAEGAASGIDIGRQVKEQIG
FDTKVTILGHLQRGGTPTAFDRILAARLGAKAVELLMAGETNKMVGIKAGEIVATDLTEVIGKPSPINKNMYELAKILSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=347, Percent_Identity=41.2103746397695, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI48762920, Length=345, Percent_Identity=41.4492753623188, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI266453619, Length=347, Percent_Identity=38.9048991354467, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI266453768, Length=347, Percent_Identity=38.9048991354467, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI266453748, Length=347, Percent_Identity=38.9048991354467, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI4505749, Length=347, Percent_Identity=38.9048991354467, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1790350, Length=319, Percent_Identity=47.9623824451411, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25147584, Length=308, Percent_Identity=43.8311688311688, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17558788, Length=321, Percent_Identity=36.7601246105919, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=30.4498269896194, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6321679, Length=340, Percent_Identity=38.8235294117647, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6323861, Length=339, Percent_Identity=36.8731563421829, Blast_Score=207, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24652337, Length=347, Percent_Identity=39.4812680115274, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI28573326, Length=347, Percent_Identity=39.4812680115274, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI17647809, Length=347, Percent_Identity=39.4812680115274, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 33820; Mature: 33820

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRIGLLTSGGDASGMNSSIRAVVRKAIYHGLEVIGIRRGYSGFIEADMGPMNLGSVADI
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCCHHHHHH
IGRGGTILHTARSERFKTKEGRALAMENIQRFGIQGLVVIGGDGSFTGASILHQEYGIPM
HCCCCEEEEECHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCE
IGVPGTIDNDIAGTDYTIGFDTAVNTVVDAINKIRDTATSHERTFVIEVMGRNSGNIALA
EECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEE
AGLAGGAESILIPEIPFNIEEICQKLLRGIKRGKLHSIILVAEGAASGIDIGRQVKEQIG
EECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHC
FDTKVTILGHLQRGGTPTAFDRILAARLGAKAVELLMAGETNKMVGIKAGEIVATDLTEV
CCCEEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCEEHHHHHHH
IGKPSPINKNMYELAKILSI
HCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQRIGLLTSGGDASGMNSSIRAVVRKAIYHGLEVIGIRRGYSGFIEADMGPMNLGSVADI
CCCEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEECCCCCCCCHHHHHH
IGRGGTILHTARSERFKTKEGRALAMENIQRFGIQGLVVIGGDGSFTGASILHQEYGIPM
HCCCCEEEEECHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCE
IGVPGTIDNDIAGTDYTIGFDTAVNTVVDAINKIRDTATSHERTFVIEVMGRNSGNIALA
EECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEE
AGLAGGAESILIPEIPFNIEEICQKLLRGIKRGKLHSIILVAEGAASGIDIGRQVKEQIG
EECCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHC
FDTKVTILGHLQRGGTPTAFDRILAARLGAKAVELLMAGETNKMVGIKAGEIVATDLTEV
CCCEEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCEEHHHHHHH
IGKPSPINKNMYELAKILSI
HCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA