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The map label for this gene is pyk [H]

Identifier: 134300151

GI number: 134300151

Start: 2529282

End: 2531033

Strand: Reverse

Name: pyk [H]

Synonym: Dred_2312

Alternate gene names: 134300151

Gene position: 2531033-2529282 (Counterclockwise)

Preceding gene: 134300152

Following gene: 134300150

Centisome position: 70.15

GC content: 47.03

Gene sequence:

>1752_bases
ATGAGACGTACAAAGATTGTTTGCACCATCGGTCCCGCCAGTGAAGATGTGGAAACGCTGAAAAATATGATGCTGGCAGG
CATGAATGTTGCAAGATTAAACTTTTCCCATGGTACCCATGAGGACCATGCCAAGAGAATTGCTGCTATTCGCCAGTCTG
CAGATGAATTAAACAAAAATATTGCCATTCTGCTAGATACCAAGGGTCCGGAAATTCGTTTAAAGAAATTTGCCCAACCG
CCGGTTATCCTAAAACAGGATCAGCAATTTACCCTGACAACCAGGGAGGTTACCGGTGACAATACCATTGTGTCTGTAAC
TTATCAGGATTTGCCCAAGGATGTGAAGCCTGGCAGTCGGATAGCTGTGGCAGATGGGTTAATCGAAATGGTTGTGGAAT
CTGTGGAGGGACCAGATGTTCATTGTAGAGTCGTTAATGGTGGTCAACTTTCCAACCAGAAGGGAGTTAATTTACCTGGT
GTAAATGTAAACCTTCCTTCGATTACCGAAAAGGATATTGAGGACATTCGCTTTGGTATAGAGCAAAGGGTAGATTTTAT
TGCTGCATCCTTTATTCGCAAACCCGGGGATGTCCTCGCCATTCGCCAAATCCTTGAGGAATATGATGCGGATATTGATA
TCATAGCTAAGATTGAAAGTCGCCAGGGTGTGGAGAATCTGACTGGTATTATTAATGTTGCAGATGGTATCATGGTGGCC
AGAGGTGACTTGGGCGTTGAAATCCCTGCAGAAGAAGTACCTGTCTTACAAAAGACGATGATTGAAATGTGCAACAGGGC
AGGTAAACCAGTTATTACTGCTACACAAATGCTGGAGTCCATGACCCAGAATCCAAGACCAACCAGGGCCGAGGCCAGCG
ATGTGGCTAATGCCATTTTTGATGGAACCGATGCAATTATGCTTTCCGGCGAAACAGCTGCGGGTAAGTATCCTGTACAA
ACCGTTGAAACCATGGCTCGTATTGCCCAGCGGGCAGAGCTTGCTGTAAATTTTGATGACCTGCTGGAAATCAGAGGAGC
TGCCATACAGAGAACGGTCACCGATGGTATTAGCCAGGCCGTTTGCAGTGTGGCTAGGGAATTGGGTGTAACCGCCATTG
TAACCGCGACGGCCAGCGGACATACCGCCAGAATGATTTCCAAGTATAGGCCCAAAGTACCTGTGATTGCTGTGACACCC
AGATCCAATGTGTTAAGAAAAATGGCTCTGGTTTGGGGGGTTGTTCCTCTTTTAATTGAAGATGTACATGGTACAGATGA
AATGATTAATATATCCGTGGAAGCGGCCCTGGCCTCCGGACATATTACTGCTGGCGACCTAATTGTTATTACTGCCGGTG
TACCGGTGGGTGTTCATGGTACCACCAATATGATGAAGGTTCACACAGTGGGGACTCTGCTGGCCAAAGGAACAGGCATT
GGCCAGAAAGCTGTCACAGGTAAGGTTAGGATTTGTCGAACGGCGGAGGAAGCCATGGTTAAGGTTCAGCCGGGTGATAT
TTTGGTGACTATGGCCACTGATCGTGACTTCGTGTCTGCTATGCAGAAAGCCAAAGCCGTGATAACGGAAGAAGGTGGTC
TTACTTCCCATGCTGCCATTGTGGGACTGGAATTTGGCTTACCCGTTATAGTAGGCGTTGATAACGCCACTGGTATTCTA
CCAGACGATGAAATTATTACGGTGGATAGCCAGCGAGGGCTGATTTACGGCGGTGTTGCCAGAGTTTTATAG

Upstream 100 bases:

>100_bases
CCAGCCCCATTAATAAGAACATGTACGAATTAGCAAAGATATTATCCATATAGAAAAAACAGTAACTATATTTTTGTTTT
TAGCAAGGGAGGTTCTGTGG

Downstream 100 bases:

>100_bases
TATAAGCGAAGGGGGCTGTTGCACAATGAACCAGAGTGCAACAACCCCTTTCAATTTCTAAATATAAAATGCGGGTCCCG
AAGAACCTACCAGACGGCGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK; Vegetative protein 17; VEG17 [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MRRTKIVCTIGPASEDVETLKNMMLAGMNVARLNFSHGTHEDHAKRIAAIRQSADELNKNIAILLDTKGPEIRLKKFAQP
PVILKQDQQFTLTTREVTGDNTIVSVTYQDLPKDVKPGSRIAVADGLIEMVVESVEGPDVHCRVVNGGQLSNQKGVNLPG
VNVNLPSITEKDIEDIRFGIEQRVDFIAASFIRKPGDVLAIRQILEEYDADIDIIAKIESRQGVENLTGIINVADGIMVA
RGDLGVEIPAEEVPVLQKTMIEMCNRAGKPVITATQMLESMTQNPRPTRAEASDVANAIFDGTDAIMLSGETAAGKYPVQ
TVETMARIAQRAELAVNFDDLLEIRGAAIQRTVTDGISQAVCSVARELGVTAIVTATASGHTARMISKYRPKVPVIAVTP
RSNVLRKMALVWGVVPLLIEDVHGTDEMINISVEAALASGHITAGDLIVITAGVPVGVHGTTNMMKVHTVGTLLAKGTGI
GQKAVTGKVRICRTAEEAMVKVQPGDILVTMATDRDFVSAMQKAKAVITEEGGLTSHAAIVGLEFGLPVIVGVDNATGIL
PDDEIITVDSQRGLIYGGVARVL

Sequences:

>Translated_583_residues
MRRTKIVCTIGPASEDVETLKNMMLAGMNVARLNFSHGTHEDHAKRIAAIRQSADELNKNIAILLDTKGPEIRLKKFAQP
PVILKQDQQFTLTTREVTGDNTIVSVTYQDLPKDVKPGSRIAVADGLIEMVVESVEGPDVHCRVVNGGQLSNQKGVNLPG
VNVNLPSITEKDIEDIRFGIEQRVDFIAASFIRKPGDVLAIRQILEEYDADIDIIAKIESRQGVENLTGIINVADGIMVA
RGDLGVEIPAEEVPVLQKTMIEMCNRAGKPVITATQMLESMTQNPRPTRAEASDVANAIFDGTDAIMLSGETAAGKYPVQ
TVETMARIAQRAELAVNFDDLLEIRGAAIQRTVTDGISQAVCSVARELGVTAIVTATASGHTARMISKYRPKVPVIAVTP
RSNVLRKMALVWGVVPLLIEDVHGTDEMINISVEAALASGHITAGDLIVITAGVPVGVHGTTNMMKVHTVGTLLAKGTGI
GQKAVTGKVRICRTAEEAMVKVQPGDILVTMATDRDFVSAMQKAKAVITEEGGLTSHAAIVGLEFGLPVIVGVDNATGIL
PDDEIITVDSQRGLIYGGVARVL
>Mature_583_residues
MRRTKIVCTIGPASEDVETLKNMMLAGMNVARLNFSHGTHEDHAKRIAAIRQSADELNKNIAILLDTKGPEIRLKKFAQP
PVILKQDQQFTLTTREVTGDNTIVSVTYQDLPKDVKPGSRIAVADGLIEMVVESVEGPDVHCRVVNGGQLSNQKGVNLPG
VNVNLPSITEKDIEDIRFGIEQRVDFIAASFIRKPGDVLAIRQILEEYDADIDIIAKIESRQGVENLTGIINVADGIMVA
RGDLGVEIPAEEVPVLQKTMIEMCNRAGKPVITATQMLESMTQNPRPTRAEASDVANAIFDGTDAIMLSGETAAGKYPVQ
TVETMARIAQRAELAVNFDDLLEIRGAAIQRTVTDGISQAVCSVARELGVTAIVTATASGHTARMISKYRPKVPVIAVTP
RSNVLRKMALVWGVVPLLIEDVHGTDEMINISVEAALASGHITAGDLIVITAGVPVGVHGTTNMMKVHTVGTLLAKGTGI
GQKAVTGKVRICRTAEEAMVKVQPGDILVTMATDRDFVSAMQKAKAVITEEGGLTSHAAIVGLEFGLPVIVGVDNATGIL
PDDEIITVDSQRGLIYGGVARVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=44.6502057613169, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=44.6502057613169, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI33286418, Length=486, Percent_Identity=45.4732510288066, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=44.5807770961145, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=44.5807770961145, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=49.8257839721254, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=49.8257839721254, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=46.8085106382979, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=46.8085106382979, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=44.392523364486, Blast_Score=169, Evalue=8e-42,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=48.8372093023256, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1788160, Length=487, Percent_Identity=39.0143737166324, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1787994, Length=137, Percent_Identity=37.2262773722628, Blast_Score=86, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=47.3029045643153, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=44.35318275154, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=44.35318275154, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=44.35318275154, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=44.35318275154, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17564524, Length=127, Percent_Identity=33.0708661417323, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319279, Length=493, Percent_Identity=43.6105476673428, Blast_Score=358, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=41.6837782340862, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=46.1224489795918, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=46.1224489795918, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=47.9318734793187, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=35.8024691358025, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24646914, Length=304, Percent_Identity=41.4473684210526, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62307; Mature: 62307

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKIVCTIGPASEDVETLKNMMLAGMNVARLNFSHGTHEDHAKRIAAIRQSADELNKN
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
IAILLDTKGPEIRLKKFAQPPVILKQDQQFTLTTREVTGDNTIVSVTYQDLPKDVKPGSR
EEEEEECCCCCCHHHHCCCCCEEEECCCEEEEEEEEECCCCEEEEEEHHHCCCCCCCCCE
IAVADGLIEMVVESVEGPDVHCRVVNGGQLSNQKGVNLPGVNVNLPSITEKDIEDIRFGI
EEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EQRVDFIAASFIRKPGDVLAIRQILEEYDADIDIIAKIESRQGVENLTGIINVADGIMVA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHECCCCEEEE
RGDLGVEIPAEEVPVLQKTMIEMCNRAGKPVITATQMLESMTQNPRPTRAEASDVANAIF
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGTDAIMLSGETAAGKYPVQTVETMARIAQRAELAVNFDDLLEIRGAAIQRTVTDGISQA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEECHHHHHHHCCHHHHHHHHHHHHHH
VCSVARELGVTAIVTATASGHTARMISKYRPKVPVIAVTPRSNVLRKMALVWGVVPLLIE
HHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
DVHGTDEMINISVEAALASGHITAGDLIVITAGVPVGVHGTTNMMKVHTVGTLLAKGTGI
HCCCCCCEEEEEEEEEEECCCCCCCCEEEEEECCEECCCCCCCEEEEEHHHHHHHCCCCC
GQKAVTGKVRICRTAEEAMVKVQPGDILVTMATDRDFVSAMQKAKAVITEEGGLTSHAAI
CCHHCCCCEEEEEEHHHCEEEECCCCEEEEEECCHHHHHHHHHHHHEEECCCCCCCCEEE
VGLEFGLPVIVGVDNATGILPDDEIITVDSQRGLIYGGVARVL
EEEECCCEEEEECCCCCCCCCCCCEEEEECCCCEEECCHHHCC
>Mature Secondary Structure
MRRTKIVCTIGPASEDVETLKNMMLAGMNVARLNFSHGTHEDHAKRIAAIRQSADELNKN
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
IAILLDTKGPEIRLKKFAQPPVILKQDQQFTLTTREVTGDNTIVSVTYQDLPKDVKPGSR
EEEEEECCCCCCHHHHCCCCCEEEECCCEEEEEEEEECCCCEEEEEEHHHCCCCCCCCCE
IAVADGLIEMVVESVEGPDVHCRVVNGGQLSNQKGVNLPGVNVNLPSITEKDIEDIRFGI
EEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
EQRVDFIAASFIRKPGDVLAIRQILEEYDADIDIIAKIESRQGVENLTGIINVADGIMVA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHECCCCEEEE
RGDLGVEIPAEEVPVLQKTMIEMCNRAGKPVITATQMLESMTQNPRPTRAEASDVANAIF
ECCCCCCCCHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DGTDAIMLSGETAAGKYPVQTVETMARIAQRAELAVNFDDLLEIRGAAIQRTVTDGISQA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEECHHHHHHHCCHHHHHHHHHHHHHH
VCSVARELGVTAIVTATASGHTARMISKYRPKVPVIAVTPRSNVLRKMALVWGVVPLLIE
HHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
DVHGTDEMINISVEAALASGHITAGDLIVITAGVPVGVHGTTNMMKVHTVGTLLAKGTGI
HCCCCCCEEEEEEEEEEECCCCCCCCEEEEEECCEECCCCCCCEEEEEHHHHHHHCCCCC
GQKAVTGKVRICRTAEEAMVKVQPGDILVTMATDRDFVSAMQKAKAVITEEGGLTSHAAI
CCHHCCCCEEEEEEHHHCEEEECCCCEEEEEECCHHHHHHHHHHHHEEECCCCCCCCEEE
VGLEFGLPVIVGVDNATGILPDDEIITVDSQRGLIYGGVARVL
EEEECCCEEEEECCCCCCCCCCCCEEEEECCCCEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 9298659 [H]