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The map label for this gene is kinE [H]

Identifier: 134300143

GI number: 134300143

Start: 2518681

End: 2520063

Strand: Reverse

Name: kinE [H]

Synonym: Dred_2304

Alternate gene names: 134300143

Gene position: 2520063-2518681 (Counterclockwise)

Preceding gene: 134300144

Following gene: 134300142

Centisome position: 69.84

GC content: 37.67

Gene sequence:

>1383_bases
ATGTTGTGTTGTCCAATTATAAAAAACAAAACAATATTTGTTTTATTCCTCTGCAATTTATTACTCTTTAGTATTTTATA
TTACGCACATATATGCTATGCAGAGGTAAATGGAAAGGATCAACCAGGCTCTCCTGTAGTAAATCAAGGAAACATCTTCT
CCAACGACCAAACCGTTCATACGCAGTATTCTAATTTCTATGAAATAAACAAAGGCCTAGTGTGGGTGGGATTGCTTACC
AGTGTTGCGGGATTTTTCTTAACAGTTTTTCTTTTTCGTAAAAATAAAGAGTTAACAAAGTCTCAGTTGTCATTAAAGCA
ATCGGAGCAGCGTTTTGCCAGAGTATTTCATGCCAATCCAAACATTCAGACCATTAATAGACTGGATGATTTTAGGTTTA
TCGATGTTAACGATAGTTTTGAAAAGATTTTTGGTTACGGAAGAGAAGAAGTCATTGGATATACACCTCTGGATTTTAAT
CTTTGGGTGAACCCACAATTGCAACAGGAACTTTTAACGCTACTGGGTAATGAAGGTCATTATACTGATAAAGAAGTTAT
ACTACGTACAAATTCAGGGGAGGAAAAGGTGCTACAGGGTTCTGGTGTAGTGGTAGATGTAACCGGAGTTTCCTGTGTAC
TGATATCCCTTAGAGATATAACAGAGTCAAAGCAAATGGAAAAGGAAATCAAAAAACTGGACAGGTTGAACCTGGTTGGA
CAAGTGGCTGCTGGTATTGGTCATGAAATCCGCAATCCATTAACCACCGTAAGAGGTTTTTTACAACTTTTTCAAACCAA
GAAAGAAAGTTGTGATATCTATAAAGAACAGTTCATGCTGATGATTGAAGAACTTGATCGAGCTAATTCTATTATCACAG
AATTTCTCACACTGGCCAGAAACAAACCTGAAAGGGTCGAGATGAAGAATCTCAATTCCATTGTCACGAAAATTTTGCCC
TTGCTACAAGCCCAGGCTTTAAAGGAAGAAAAGTATGTCTATGCAGTTTTAGACAAGGTACCCGATTTGCTGTTGGACGA
AAATGAAATAAGACAAATTATTCTCAATTTAATTCGGAATGGTCTGGATGCCATGTCAAGGGGAGGGAACATTACCATAC
GTACCTATATCCAAGAACCTCATACCATATTATCTATTCGTGATGAAGGGGAAGGCATTAAGCCCGAAGTTCTCGAAAAG
ATAGGTACGCCTTTTTATACCACAAAGGATAGTGGAACTGGCTTAGGGCTGGCAATCTGTTATGGCATTGCAGAGAGACA
CAATGCTTCAGTGGATGTAAAAACAGGCCCTAATGGTACAATCTTTCTGATAAAATTCCCCTTTGATAATCAAAACCAAT
TGTGTAATACGAAGGTAAGCTAA

Upstream 100 bases:

>100_bases
TCCAACATGCATAGGGCTATTAAGGACGTCACCTAGAACCTAAAAGTCTGGTACCCCATAGGTTTGACACCAGCCAAGTT
TTTTAAAAAGGATGATGACA

Downstream 100 bases:

>100_bases
AATAGAGTGTCTATTGGTTGTGAACCGGGACAGATGTCCTATTTTAAAGATGAAACAAAGTCTATATAATTTAAATTAAC
ATAATAAAGAAAGGCAAACC

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLCCPIIKNKTIFVLFLCNLLLFSILYYAHICYAEVNGKDQPGSPVVNQGNIFSNDQTVHTQYSNFYEINKGLVWVGLLT
SVAGFFLTVFLFRKNKELTKSQLSLKQSEQRFARVFHANPNIQTINRLDDFRFIDVNDSFEKIFGYGREEVIGYTPLDFN
LWVNPQLQQELLTLLGNEGHYTDKEVILRTNSGEEKVLQGSGVVVDVTGVSCVLISLRDITESKQMEKEIKKLDRLNLVG
QVAAGIGHEIRNPLTTVRGFLQLFQTKKESCDIYKEQFMLMIEELDRANSIITEFLTLARNKPERVEMKNLNSIVTKILP
LLQAQALKEEKYVYAVLDKVPDLLLDENEIRQIILNLIRNGLDAMSRGGNITIRTYIQEPHTILSIRDEGEGIKPEVLEK
IGTPFYTTKDSGTGLGLAICYGIAERHNASVDVKTGPNGTIFLIKFPFDNQNQLCNTKVS

Sequences:

>Translated_460_residues
MLCCPIIKNKTIFVLFLCNLLLFSILYYAHICYAEVNGKDQPGSPVVNQGNIFSNDQTVHTQYSNFYEINKGLVWVGLLT
SVAGFFLTVFLFRKNKELTKSQLSLKQSEQRFARVFHANPNIQTINRLDDFRFIDVNDSFEKIFGYGREEVIGYTPLDFN
LWVNPQLQQELLTLLGNEGHYTDKEVILRTNSGEEKVLQGSGVVVDVTGVSCVLISLRDITESKQMEKEIKKLDRLNLVG
QVAAGIGHEIRNPLTTVRGFLQLFQTKKESCDIYKEQFMLMIEELDRANSIITEFLTLARNKPERVEMKNLNSIVTKILP
LLQAQALKEEKYVYAVLDKVPDLLLDENEIRQIILNLIRNGLDAMSRGGNITIRTYIQEPHTILSIRDEGEGIKPEVLEK
IGTPFYTTKDSGTGLGLAICYGIAERHNASVDVKTGPNGTIFLIKFPFDNQNQLCNTKVS
>Mature_460_residues
MLCCPIIKNKTIFVLFLCNLLLFSILYYAHICYAEVNGKDQPGSPVVNQGNIFSNDQTVHTQYSNFYEINKGLVWVGLLT
SVAGFFLTVFLFRKNKELTKSQLSLKQSEQRFARVFHANPNIQTINRLDDFRFIDVNDSFEKIFGYGREEVIGYTPLDFN
LWVNPQLQQELLTLLGNEGHYTDKEVILRTNSGEEKVLQGSGVVVDVTGVSCVLISLRDITESKQMEKEIKKLDRLNLVG
QVAAGIGHEIRNPLTTVRGFLQLFQTKKESCDIYKEQFMLMIEELDRANSIITEFLTLARNKPERVEMKNLNSIVTKILP
LLQAQALKEEKYVYAVLDKVPDLLLDENEIRQIILNLIRNGLDAMSRGGNITIRTYIQEPHTILSIRDEGEGIKPEVLEK
IGTPFYTTKDSGTGLGLAICYGIAERHNASVDVKTGPNGTIFLIKFPFDNQNQLCNTKVS

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=323, Percent_Identity=29.1021671826625, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790436, Length=277, Percent_Identity=27.7978339350181, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1790300, Length=354, Percent_Identity=24.8587570621469, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786600, Length=246, Percent_Identity=26.4227642276423, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786783, Length=216, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789808, Length=237, Percent_Identity=25.7383966244726, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI145693157, Length=269, Percent_Identity=22.6765799256506, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788393, Length=224, Percent_Identity=25, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790346, Length=211, Percent_Identity=27.0142180094787, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52144; Mature: 52144

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCCPIIKNKTIFVLFLCNLLLFSILYYAHICYAEVNGKDQPGSPVVNQGNIFSNDQTVH
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEECCCCEEE
TQYSNFYEINKGLVWVGLLTSVAGFFLTVFLFRKNKELTKSQLSLKQSEQRFARVFHANP
ECCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
NIQTINRLDDFRFIDVNDSFEKIFGYGREEVIGYTPLDFNLWVNPQLQQELLTLLGNEGH
CCHHHHHCCCEEEEECCCCHHHHHCCCCHHEEEECCCEEEEEECCHHHHHHHHHHCCCCC
YTDKEVILRTNSGEEKVLQGSGVVVDVTGVSCVLISLRDITESKQMEKEIKKLDRLNLVG
CCCCEEEEEECCCCHHEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QVAAGIGHEIRNPLTTVRGFLQLFQTKKESCDIYKEQFMLMIEELDRANSIITEFLTLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKPERVEMKNLNSIVTKILPLLQAQALKEEKYVYAVLDKVPDLLLDENEIRQIILNLIRN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GLDAMSRGGNITIRTYIQEPHTILSIRDEGEGIKPEVLEKIGTPFYTTKDSGTGLGLAIC
HHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCCHHHHH
YGIAERHNASVDVKTGPNGTIFLIKFPFDNQNQLCNTKVS
HHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MLCCPIIKNKTIFVLFLCNLLLFSILYYAHICYAEVNGKDQPGSPVVNQGNIFSNDQTVH
CEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCEECCCCEEE
TQYSNFYEINKGLVWVGLLTSVAGFFLTVFLFRKNKELTKSQLSLKQSEQRFARVFHANP
ECCCCCEECCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
NIQTINRLDDFRFIDVNDSFEKIFGYGREEVIGYTPLDFNLWVNPQLQQELLTLLGNEGH
CCHHHHHCCCEEEEECCCCHHHHHCCCCHHEEEECCCEEEEEECCHHHHHHHHHHCCCCC
YTDKEVILRTNSGEEKVLQGSGVVVDVTGVSCVLISLRDITESKQMEKEIKKLDRLNLVG
CCCCEEEEEECCCCHHEEECCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QVAAGIGHEIRNPLTTVRGFLQLFQTKKESCDIYKEQFMLMIEELDRANSIITEFLTLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKPERVEMKNLNSIVTKILPLLQAQALKEEKYVYAVLDKVPDLLLDENEIRQIILNLIRN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GLDAMSRGGNITIRTYIQEPHTILSIRDEGEGIKPEVLEKIGTPFYTTKDSGTGLGLAIC
HHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCCHHHHH
YGIAERHNASVDVKTGPNGTIFLIKFPFDNQNQLCNTKVS
HHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]