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The map label for this gene is algU [H]

Identifier: 134300125

GI number: 134300125

Start: 2499164

End: 2499766

Strand: Reverse

Name: algU [H]

Synonym: Dred_2286

Alternate gene names: 134300125

Gene position: 2499766-2499164 (Counterclockwise)

Preceding gene: 134300126

Following gene: 134300124

Centisome position: 69.28

GC content: 41.79

Gene sequence:

>603_bases
TTGCCCCTGGAAGATCAGTTGCTTGTTGAACGTAGCAAAAAAGGTGATAGAGAAGCTTTTGAACATCTAGTTCGCTTATA
TGAGAATAAGGTTTATACAATTGCTTACAGATTAATGGGGAATCATGCAGATGCCTCGGATCTGGCTCAGGATGCTTTTA
TCAAGATATACCAGGCACTTCCCAATTTTCGAGGTGATTCCAGTTTTAGTACCTGGATTTATCATATTACTGTGAATGTT
TGTCGTGATGAACTGCGGAAACGGCAACGTAGGCCAACTGTTTCGTTGGATGATAACTCCAGCGACAGTAATAACAGCAA
TACATATGAAATTCGCAGCAACGACCCAGGACCTGAAGAAATGTTGGATCGTAGTGAGACCCAGGCTATGATTCAGGCGT
GTTTGAATACTCTCTCAGATGACTATCGAGAAATAATTGTTATGCGCGAGATTCAAGAATTGGCCTATGAGGAAATAGCA
GAGATTTTAGGGTGTTCCTTGGGAACAGTTAAATCCCGGCTAAGTCGTGCTCGGCAGGCCTTAAAAGAGAAAATCAGTAA
GCAAATGGAACTTATAACTCCTGCCAAACGTCTAGCTAAGTAA

Upstream 100 bases:

>100_bases
AGTATTTTTAAGTTATCTGGTTAGTTGATTGGAAACCTTTTGCTAACTTTGAAATATTCTGGAAGAGATTAATAAACCTT
TCAAAAGAGGAGGGGGACCC

Downstream 100 bases:

>100_bases
AGGAGGGATGTTCCGTGCAATGCCACGATATTTTAGGTATGCTGTCCCCTTATCTGGACGGGGTACTTAATTCAGAAGAG
TGCAATGAAGTACGGGTACA

Product: ECF subfamily RNA polymerase sigma-24 factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MPLEDQLLVERSKKGDREAFEHLVRLYENKVYTIAYRLMGNHADASDLAQDAFIKIYQALPNFRGDSSFSTWIYHITVNV
CRDELRKRQRRPTVSLDDNSSDSNNSNTYEIRSNDPGPEEMLDRSETQAMIQACLNTLSDDYREIIVMREIQELAYEEIA
EILGCSLGTVKSRLSRARQALKEKISKQMELITPAKRLAK

Sequences:

>Translated_200_residues
MPLEDQLLVERSKKGDREAFEHLVRLYENKVYTIAYRLMGNHADASDLAQDAFIKIYQALPNFRGDSSFSTWIYHITVNV
CRDELRKRQRRPTVSLDDNSSDSNNSNTYEIRSNDPGPEEMLDRSETQAMIQACLNTLSDDYREIIVMREIQELAYEEIA
EILGCSLGTVKSRLSRARQALKEKISKQMELITPAKRLAK
>Mature_199_residues
PLEDQLLVERSKKGDREAFEHLVRLYENKVYTIAYRLMGNHADASDLAQDAFIKIYQALPNFRGDSSFSTWIYHITVNVC
RDELRKRQRRPTVSLDDNSSDSNNSNTYEIRSNDPGPEEMLDRSETQAMIQACLNTLSDDYREIIVMREIQELAYEEIAE
ILGCSLGTVKSRLSRARQALKEKISKQMELITPAKRLAK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=183, Percent_Identity=40.4371584699454, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 23079; Mature: 22948

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLEDQLLVERSKKGDREAFEHLVRLYENKVYTIAYRLMGNHADASDLAQDAFIKIYQAL
CCCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
PNFRGDSSFSTWIYHITVNVCRDELRKRQRRPTVSLDDNSSDSNNSNTYEIRSNDPGPEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCCHHH
MLDRSETQAMIQACLNTLSDDYREIIVMREIQELAYEEIAEILGCSLGTVKSRLSRARQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKEKISKQMELITPAKRLAK
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLEDQLLVERSKKGDREAFEHLVRLYENKVYTIAYRLMGNHADASDLAQDAFIKIYQAL
CCCHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
PNFRGDSSFSTWIYHITVNVCRDELRKRQRRPTVSLDDNSSDSNNSNTYEIRSNDPGPEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCCHHH
MLDRSETQAMIQACLNTLSDDYREIIVMREIQELAYEEIAEILGCSLGTVKSRLSRARQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LKEKISKQMELITPAKRLAK
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]