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The map label for this gene is rnhB

Identifier: 134299893

GI number: 134299893

Start: 2246005

End: 2246601

Strand: Reverse

Name: rnhB

Synonym: Dred_2047

Alternate gene names: 134299893

Gene position: 2246601-2246005 (Counterclockwise)

Preceding gene: 134299894

Following gene: 134299892

Centisome position: 62.27

GC content: 42.55

Gene sequence:

>597_bases
ATGGTTTTGGAATGCGGTGTGGATGAGACGGGTCGTGGATCTTGTATTTCAGGAATATATGCTTCGGCTTGCATTCTTGA
TCCGGCACACCCCATTGAGGGGTTAAGGGACTCAAAAAAACTGAGTGCACGTAAGAGGGAGATTTTAGCAGAGGAAATAA
AGCAATACGCATTAAGCTGGTGCATTGCGCAGGCTAGTCTAGAAGAAGTTGAACAGCTAAATGTACATCATGCTACATTG
CTTGCTATGAAGCGGGCCATAGAAGGTCTAAGCATTAGAGCTAATAAGGTTTATGTGGATGGTATTCATTTACCAGAGGT
TGACATTCCGGCTGAAGCCATTGTAAAGGGAGATGATCTTATCCCTGCCATTAGTGCAGCTTCAATACTGGCTAAAGTAG
CTCGGGATGGAGCGATGCTTGAGTACCATGAAAAATACCCTCAATATGGATTTAACAGTCACAAGGGGTATCTAACAAAG
GCACACAGGGAGGCGCTTAAAAAATATGGACCCAGTCCAATACATAGAAAAACTTATGCTCCTATACGTGAACTTTTAGT
TGGCAAGGACAATGAACAAATTGAAATGTTTGAATGA

Upstream 100 bases:

>100_bases
AAATATAATGTTAATGATCCATGCATGTTGACTAGCTAGAATGCTATTATATTTTTGTAAGTCTAATAGGATTATAAGCA
GGGGCTAAGGAGGATAGAGT

Downstream 100 bases:

>100_bases
TTATGCTAATTCCTTCATAAGTTGAAAAATAATTTAGATAATTTTTGCAACTTGTGAAGGAATTCTTATCTTATTTGGAA
AATAATATTTATATTAATGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MVLECGVDETGRGSCISGIYASACILDPAHPIEGLRDSKKLSARKREILAEEIKQYALSWCIAQASLEEVEQLNVHHATL
LAMKRAIEGLSIRANKVYVDGIHLPEVDIPAEAIVKGDDLIPAISAASILAKVARDGAMLEYHEKYPQYGFNSHKGYLTK
AHREALKKYGPSPIHRKTYAPIRELLVGKDNEQIEMFE

Sequences:

>Translated_198_residues
MVLECGVDETGRGSCISGIYASACILDPAHPIEGLRDSKKLSARKREILAEEIKQYALSWCIAQASLEEVEQLNVHHATL
LAMKRAIEGLSIRANKVYVDGIHLPEVDIPAEAIVKGDDLIPAISAASILAKVARDGAMLEYHEKYPQYGFNSHKGYLTK
AHREALKKYGPSPIHRKTYAPIRELLVGKDNEQIEMFE
>Mature_198_residues
MVLECGVDETGRGSCISGIYASACILDPAHPIEGLRDSKKLSARKREILAEEIKQYALSWCIAQASLEEVEQLNVHHATL
LAMKRAIEGLSIRANKVYVDGIHLPEVDIPAEAIVKGDDLIPAISAASILAKVARDGAMLEYHEKYPQYGFNSHKGYLTK
AHREALKKYGPSPIHRKTYAPIRELLVGKDNEQIEMFE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=50, Blast_Score=186, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_DESRM (A4J661)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113389.1
- ProteinModelPortal:   A4J661
- SMR:   A4J661
- STRING:   A4J661
- GeneID:   4958639
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2047
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- PhylomeDB:   A4J661
- ProtClustDB:   PRK00015
- BioCyc:   DRED349161:DRED_2047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21951; Mature: 21951

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLECGVDETGRGSCISGIYASACILDPAHPIEGLRDSKKLSARKREILAEEIKQYALSW
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
CIAQASLEEVEQLNVHHATLLAMKRAIEGLSIRANKVYVDGIHLPEVDIPAEAIVKGDDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCEECCCCCCCHHHHHCCCCC
IPAISAASILAKVARDGAMLEYHEKYPQYGFNSHKGYLTKAHREALKKYGPSPIHRKTYA
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
PIRELLVGKDNEQIEMFE
HHHHHHHCCCCCCEECCC
>Mature Secondary Structure
MVLECGVDETGRGSCISGIYASACILDPAHPIEGLRDSKKLSARKREILAEEIKQYALSW
CEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
CIAQASLEEVEQLNVHHATLLAMKRAIEGLSIRANKVYVDGIHLPEVDIPAEAIVKGDDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCEECCCCCCCHHHHHCCCCC
IPAISAASILAKVARDGAMLEYHEKYPQYGFNSHKGYLTKAHREALKKYGPSPIHRKTYA
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
PIRELLVGKDNEQIEMFE
HHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA