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The map label for this gene is ndhF [H]

Identifier: 134299884

GI number: 134299884

Start: 2238583

End: 2240520

Strand: Reverse

Name: ndhF [H]

Synonym: Dred_2038

Alternate gene names: 134299884

Gene position: 2240520-2238583 (Counterclockwise)

Preceding gene: 134299885

Following gene: 134299883

Centisome position: 62.1

GC content: 45.56

Gene sequence:

>1938_bases
ATGGTTGAATTTGCTTTGGGTCATGCTTGGCTGGTACCTTTGTTACCTGCAATGGCCTTTGCCATTATTGTCTTTCTTAC
CAGGCCCTTACCAAAACTAAGCTCATCGATTTCTGTTTTAGCAATGGCATCTTCTTTTGTTTTGGCTGCAGCCATTGCTT
ACGGAGTATTTACCAATCCACACTTTATTGAAGAGCCGTTGGTTTATTCTCTGAGATGGTTTGGTATGGAAGAGTTTACG
GTAAAGGTTGGGGTAATGCTGGATCCCACCTCTGCCATGATGCTTTTCATGGTTTCTTTGGTGGCAACTCTGATTCAGAT
ATATTCCACGGGCTACATGGAGGGAGACCCTCAGTACTCAGTATTTTTCTCTTACATGTCACTCTTTGCAGCCTCTATGT
TGGGATTAGTTATTTCCAGCAATTTGTTACAAATGTTTGTGATGTGGGAACTGGTAGGTCTTTGTTCTTATCTGCTGATT
GGTTTTTATACCTTTAAAATTTCTGCCCGGGAGGCAGCCAAAAAGGCCTTTATGACCACCCGAATTGGTGACTTTGGTAT
GCTTTTGGGTTTGCTTTTTTTACAGATTCTCTTTGGCACCTTGGATTTAACCGAGTTGGCCCAGAGGGTTCCCAACTATG
AACAATTAGGCATCTCCATTGGTTTGCTGACTCTTATTGCAATATTGGTATTTATCGGTCCCATTGGTAAGTCCGGTCAG
TTCCCATTGCACGTATGGTTGCCGGATGCAATGGAAGGACCAACACCTGTATCAGCCTTGATTCACGCTGCTACCATGGT
GGTTGCAGGGGTTTATTTGGTAGGGAGAACACTCTTCCTCTTTGCTGAAGTTCCAGGAGCCATGGAGGTTGTGGCCTTTA
CAGGGGCCTTTACAGCCCTATTTGCTGCCACCATCGCCATTACGCAAAGAGAAATAAAGCGTATCCTGGCCTACTCCACC
GTATCCCAGTTGGGCTACATGATGCTGGCCTTAGGGGTCGGGAGTCTTTCAGCCAGTATGTTCCACCTCTGGACCCATGC
CTTCTTTAAGGCATTGATGTTCTTGGCGGCAGGTTCAGTATTACACGCTTTACATGATAAGGCTGATGTTTGGAAGATGG
GTGGATTGATTAAAAAGATGCCCATCACGGGTTGGACCTTTGTGATAGGGGGGTTAGCCATTGCAGGTATCCCTCCCTTT
GCAGGATTTTGGTCCAAAGACGAAATCCTTGCCGTAACGCTGCAATATGCCAACCACGGACATGGCTTTGGATATGTTCT
ATTCTTTATGGCAGCCTTTACGGCCTTTCTTACAGCCTTTTATATGTGGCGCATGATATTCTTGGCCTTCTTTGGTCAAG
AAAAACCGGAAAATCACCCCCATGAGTCTCCCTTAAACATGACTTTTCCGCTGATGGTACTGGCAGGCTTTGCCACCGTA
GGAGGTCTGGTTGGTACACCATGGGCAAACCTCTGGGGTGAGTGGATTCATTTTGGACAGCCCCATCATGGGGAACCAGC
CTACGGGTTAATGCTGTTATCAGTGGTTATTGCTGTAGCAGGCATAGGTTTGGCTTATATGCTCTACTTGAAGGATGAGG
AAAAACAAAGGGCTAAGCAATTGGCAGAGAAGTACCAGGCCATCTACAACCTTTCTTACAATAAATATTATATCGATGAA
CTTTACCAGTGGTTCACTAGAAAAGTTGTGGATGCCGGGGCTAAAGTGCTGTATTGGTTTGATATTTACTTCGTGGATGG
TGTTGTTAACGGACTGGCTAAATTTACTAGGGTGTCCGGGCAGGGTTTTAGATATTTTCAAACTGGTAAAATGCAGACCT
ACGCCTTGTTTTTCTTCCTCGGGTTGCTGGTAATAACTGTGGTGCTGGCCTTCGGAGATTCCACCTCAGCCGGTATCATG
GGAGGTGTTAAATCATGA

Upstream 100 bases:

>100_bases
CAATTGTCATTGCCATCTTTAGAAATAGGCATTCGGTAAACTTGGATGACTTTGATCTGATGAAATGGTAGGACACGGGT
TGGAAGGTAGGTGTTAACAG

Downstream 100 bases:

>100_bases
GTATCCCGGTACTAACTATAACCTTGCTTTCTCCTATGCTGGCTGCCTTTGTTATTATGTTTATCCCAAAGGAAGAAGAA
AAGCTTTGTAAATGGGTAGC

Product: proton-translocating NADH-quinone oxidoreductase, chain L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MVEFALGHAWLVPLLPAMAFAIIVFLTRPLPKLSSSISVLAMASSFVLAAAIAYGVFTNPHFIEEPLVYSLRWFGMEEFT
VKVGVMLDPTSAMMLFMVSLVATLIQIYSTGYMEGDPQYSVFFSYMSLFAASMLGLVISSNLLQMFVMWELVGLCSYLLI
GFYTFKISAREAAKKAFMTTRIGDFGMLLGLLFLQILFGTLDLTELAQRVPNYEQLGISIGLLTLIAILVFIGPIGKSGQ
FPLHVWLPDAMEGPTPVSALIHAATMVVAGVYLVGRTLFLFAEVPGAMEVVAFTGAFTALFAATIAITQREIKRILAYST
VSQLGYMMLALGVGSLSASMFHLWTHAFFKALMFLAAGSVLHALHDKADVWKMGGLIKKMPITGWTFVIGGLAIAGIPPF
AGFWSKDEILAVTLQYANHGHGFGYVLFFMAAFTAFLTAFYMWRMIFLAFFGQEKPENHPHESPLNMTFPLMVLAGFATV
GGLVGTPWANLWGEWIHFGQPHHGEPAYGLMLLSVVIAVAGIGLAYMLYLKDEEKQRAKQLAEKYQAIYNLSYNKYYIDE
LYQWFTRKVVDAGAKVLYWFDIYFVDGVVNGLAKFTRVSGQGFRYFQTGKMQTYALFFFLGLLVITVVLAFGDSTSAGIM
GGVKS

Sequences:

>Translated_645_residues
MVEFALGHAWLVPLLPAMAFAIIVFLTRPLPKLSSSISVLAMASSFVLAAAIAYGVFTNPHFIEEPLVYSLRWFGMEEFT
VKVGVMLDPTSAMMLFMVSLVATLIQIYSTGYMEGDPQYSVFFSYMSLFAASMLGLVISSNLLQMFVMWELVGLCSYLLI
GFYTFKISAREAAKKAFMTTRIGDFGMLLGLLFLQILFGTLDLTELAQRVPNYEQLGISIGLLTLIAILVFIGPIGKSGQ
FPLHVWLPDAMEGPTPVSALIHAATMVVAGVYLVGRTLFLFAEVPGAMEVVAFTGAFTALFAATIAITQREIKRILAYST
VSQLGYMMLALGVGSLSASMFHLWTHAFFKALMFLAAGSVLHALHDKADVWKMGGLIKKMPITGWTFVIGGLAIAGIPPF
AGFWSKDEILAVTLQYANHGHGFGYVLFFMAAFTAFLTAFYMWRMIFLAFFGQEKPENHPHESPLNMTFPLMVLAGFATV
GGLVGTPWANLWGEWIHFGQPHHGEPAYGLMLLSVVIAVAGIGLAYMLYLKDEEKQRAKQLAEKYQAIYNLSYNKYYIDE
LYQWFTRKVVDAGAKVLYWFDIYFVDGVVNGLAKFTRVSGQGFRYFQTGKMQTYALFFFLGLLVITVVLAFGDSTSAGIM
GGVKS
>Mature_645_residues
MVEFALGHAWLVPLLPAMAFAIIVFLTRPLPKLSSSISVLAMASSFVLAAAIAYGVFTNPHFIEEPLVYSLRWFGMEEFT
VKVGVMLDPTSAMMLFMVSLVATLIQIYSTGYMEGDPQYSVFFSYMSLFAASMLGLVISSNLLQMFVMWELVGLCSYLLI
GFYTFKISAREAAKKAFMTTRIGDFGMLLGLLFLQILFGTLDLTELAQRVPNYEQLGISIGLLTLIAILVFIGPIGKSGQ
FPLHVWLPDAMEGPTPVSALIHAATMVVAGVYLVGRTLFLFAEVPGAMEVVAFTGAFTALFAATIAITQREIKRILAYST
VSQLGYMMLALGVGSLSASMFHLWTHAFFKALMFLAAGSVLHALHDKADVWKMGGLIKKMPITGWTFVIGGLAIAGIPPF
AGFWSKDEILAVTLQYANHGHGFGYVLFFMAAFTAFLTAFYMWRMIFLAFFGQEKPENHPHESPLNMTFPLMVLAGFATV
GGLVGTPWANLWGEWIHFGQPHHGEPAYGLMLLSVVIAVAGIGLAYMLYLKDEEKQRAKQLAEKYQAIYNLSYNKYYIDE
LYQWFTRKVVDAGAKVLYWFDIYFVDGVVNGLAKFTRVSGQGFRYFQTGKMQTYALFFFLGLLVITVVLAFGDSTSAGIM
GGVKS

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=404, Percent_Identity=38.1188118811881, Blast_Score=254, Evalue=2e-67,
Organism=Escherichia coli, GI1788614, Length=613, Percent_Identity=38.4991843393148, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI1788829, Length=343, Percent_Identity=34.402332361516, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1788827, Length=437, Percent_Identity=30.2059496567506, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1788831, Length=364, Percent_Identity=30.7692307692308, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI2367154, Length=429, Percent_Identity=26.8065268065268, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788613, Length=402, Percent_Identity=25.6218905472637, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693160, Length=319, Percent_Identity=26.6457680250784, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 71204; Mature: 71204

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEFALGHAWLVPLLPAMAFAIIVFLTRPLPKLSSSISVLAMASSFVLAAAIAYGVFTNP
CCEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HFIEEPLVYSLRWFGMEEFTVKVGVMLDPTSAMMLFMVSLVATLIQIYSTGYMEGDPQYS
CHHCCHHHHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VFFSYMSLFAASMLGLVISSNLLQMFVMWELVGLCSYLLIGFYTFKISAREAAKKAFMTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHH
RIGDFGMLLGLLFLQILFGTLDLTELAQRVPNYEQLGISIGLLTLIAILVFIGPIGKSGQ
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FPLHVWLPDAMEGPTPVSALIHAATMVVAGVYLVGRTLFLFAEVPGAMEVVAFTGAFTAL
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FAATIAITQREIKRILAYSTVSQLGYMMLALGVGSLSASMFHLWTHAFFKALMFLAAGSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHALHDKADVWKMGGLIKKMPITGWTFVIGGLAIAGIPPFAGFWSKDEILAVTLQYANHG
HHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCC
HGFGYVLFFMAAFTAFLTAFYMWRMIFLAFFGQEKPENHPHESPLNMTFPLMVLAGFATV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GGLVGTPWANLWGEWIHFGQPHHGEPAYGLMLLSVVIAVAGIGLAYMLYLKDEEKQRAKQ
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LAEKYQAIYNLSYNKYYIDELYQWFTRKVVDAGAKVLYWFDIYFVDGVVNGLAKFTRVSG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
QGFRYFQTGKMQTYALFFFLGLLVITVVLAFGDSTSAGIMGGVKS
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVEFALGHAWLVPLLPAMAFAIIVFLTRPLPKLSSSISVLAMASSFVLAAAIAYGVFTNP
CCEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HFIEEPLVYSLRWFGMEEFTVKVGVMLDPTSAMMLFMVSLVATLIQIYSTGYMEGDPQYS
CHHCCHHHHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VFFSYMSLFAASMLGLVISSNLLQMFVMWELVGLCSYLLIGFYTFKISAREAAKKAFMTT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHH
RIGDFGMLLGLLFLQILFGTLDLTELAQRVPNYEQLGISIGLLTLIAILVFIGPIGKSGQ
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FPLHVWLPDAMEGPTPVSALIHAATMVVAGVYLVGRTLFLFAEVPGAMEVVAFTGAFTAL
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FAATIAITQREIKRILAYSTVSQLGYMMLALGVGSLSASMFHLWTHAFFKALMFLAAGSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHALHDKADVWKMGGLIKKMPITGWTFVIGGLAIAGIPPFAGFWSKDEILAVTLQYANHG
HHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCC
HGFGYVLFFMAAFTAFLTAFYMWRMIFLAFFGQEKPENHPHESPLNMTFPLMVLAGFATV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GGLVGTPWANLWGEWIHFGQPHHGEPAYGLMLLSVVIAVAGIGLAYMLYLKDEEKQRAKQ
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LAEKYQAIYNLSYNKYYIDELYQWFTRKVVDAGAKVLYWFDIYFVDGVVNGLAKFTRVSG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
QGFRYFQTGKMQTYALFFFLGLLVITVVLAFGDSTSAGIMGGVKS
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]