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The map label for this gene is ndhD1 [H]

Identifier: 134299883

GI number: 134299883

Start: 2237072

End: 2238586

Strand: Reverse

Name: ndhD1 [H]

Synonym: Dred_2037

Alternate gene names: 134299883

Gene position: 2238586-2237072 (Counterclockwise)

Preceding gene: 134299884

Following gene: 134299882

Centisome position: 62.04

GC content: 45.68

Gene sequence:

>1515_bases
ATGAGTATCCCGGTACTAACTATAACCTTGCTTTCTCCTATGCTGGCTGCCTTTGTTATTATGTTTATCCCAAAGGAAGA
AGAAAAGCTTTGTAAATGGGTAGCTGCCGCTGGTACAGGAACTGCGTTGCTCCTCAGCTTGTACGTTTATTTTGGGTACG
ACCAAACCTTGGGAGGCCTACAATGGGTAGATTTCGGCGGACCCATTCCGTGGATTCGAGACCTTGGGGTGTCCTATTAT
ATGGCAGTGGACGGCTTGAGTTTACCGATGCTGCTGCTGACCAATCTAATCGGTTTTTCAGCCGTGTTTGCATCTTGGAA
TGTAACACTAAGGGCCAAGGAACTGATGATTTATTTACTTATTTTAATTGCTGGTGTTATGGGAACCTTTATAGCCCAAG
ACTTGTTTATCTTCTTGCTGTTCTATGAAGTAGTGGTTATTCCTATTTACATTATGGTTATCATCTGGGGGAGCTCAAAA
AGAGTTACCAAAGAATATGCCGGTATGAAGCTGACCATTTATTTGTTAATTGGATCTGCCTTCCTGCTGGTAGGGGTTAT
TGCTACCTTTGTAAAGTCTGTAGCCATAACCGGTGTACCCAATATGAGCTTTGAAGCACTGGCACAGGTTGCCTTTCCGG
AACAGTTCCAAATCTATATGTTTGCATTAATGCTAATCGGATTTGGATCACTGCTCTCCATGTGGCCCTTCCATTCCTGG
TCACCGGATGGCTATGCTGGTGCCCCTACTGCAGTTTCTATGATTCACGCAGGGGTATTAAAGAAAATTGGGGGATACGG
TCTGATTCGGTTTGCTGTTTTTGTACTGCCCTTGGGTGCTAAATTTTGGGCTCCCTATATTGCAGTTTTGGGTGTGATTG
GTGTTGCCTATGCCGCAATGGGAGCACTGGCCCAAAAAGATCTTAAATATGTGGTCGGTTATTCCTCTGTGTCTCACATG
GGATATGTACTACTGGGGGTAGCTTCCCTACAAGTGATTGGGGTTAACGGGGCTGTGGCGAATATGTTTGCCCATGGTGT
GATGGCAGCTTTGTTCTTCTCTATGATTGGATTTATTTATGAAAAGACCCATACCCGTTGGATTCCAGATCTAGGGGGGA
TATCCCATCAGGCACCTCGGCTGTCCGTAGGTTTTATGATGGCAGCCTTGGCTTCCTTGGGTTTACCGGGGTTAATTAGT
TTCGTGCCCGAGTTTGTAATTTTTGTACAATCCTTCAAGAGCTTTGGTTTACTGGCAGTGGTGGCCATTGCCGGAATTAT
TATAACAGCGTTGTATGTACTACGGGCCGGTGCCAACACTCTCTTTGGACCTGCCAGAGAAGAATACAACCATATTAAAG
ACATTCGGGGAGCTGAACTGGTACCGCTGGTTGTACTGGGTAGCGTTCTGGTCATTGGAGGAATACTTCCCTCACTGCTG
TTTGATATGGTGAATAGCGGGGTAGCGCCTATTCTGGCACATATTAATGAAGCCCTGCAGTTAGGGGGGAGATAA

Upstream 100 bases:

>100_bases
AGACCTACGCCTTGTTTTTCTTCCTCGGGTTGCTGGTAATAACTGTGGTGCTGGCCTTCGGAGATTCCACCTCAGCCGGT
ATCATGGGAGGTGTTAAATC

Downstream 100 bases:

>100_bases
AAATGGAATTTAATTTTGCCTTAATAACCACTGAACTGGCTTTGTTTATACTTGGTTTAGCCACATTTGTATTGGGGCTA
TTGGTTCCCTCCCATTCCCG

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4 1; NDH-1, chain 4 1 [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MSIPVLTITLLSPMLAAFVIMFIPKEEEKLCKWVAAAGTGTALLLSLYVYFGYDQTLGGLQWVDFGGPIPWIRDLGVSYY
MAVDGLSLPMLLLTNLIGFSAVFASWNVTLRAKELMIYLLILIAGVMGTFIAQDLFIFLLFYEVVVIPIYIMVIIWGSSK
RVTKEYAGMKLTIYLLIGSAFLLVGVIATFVKSVAITGVPNMSFEALAQVAFPEQFQIYMFALMLIGFGSLLSMWPFHSW
SPDGYAGAPTAVSMIHAGVLKKIGGYGLIRFAVFVLPLGAKFWAPYIAVLGVIGVAYAAMGALAQKDLKYVVGYSSVSHM
GYVLLGVASLQVIGVNGAVANMFAHGVMAALFFSMIGFIYEKTHTRWIPDLGGISHQAPRLSVGFMMAALASLGLPGLIS
FVPEFVIFVQSFKSFGLLAVVAIAGIIITALYVLRAGANTLFGPAREEYNHIKDIRGAELVPLVVLGSVLVIGGILPSLL
FDMVNSGVAPILAHINEALQLGGR

Sequences:

>Translated_504_residues
MSIPVLTITLLSPMLAAFVIMFIPKEEEKLCKWVAAAGTGTALLLSLYVYFGYDQTLGGLQWVDFGGPIPWIRDLGVSYY
MAVDGLSLPMLLLTNLIGFSAVFASWNVTLRAKELMIYLLILIAGVMGTFIAQDLFIFLLFYEVVVIPIYIMVIIWGSSK
RVTKEYAGMKLTIYLLIGSAFLLVGVIATFVKSVAITGVPNMSFEALAQVAFPEQFQIYMFALMLIGFGSLLSMWPFHSW
SPDGYAGAPTAVSMIHAGVLKKIGGYGLIRFAVFVLPLGAKFWAPYIAVLGVIGVAYAAMGALAQKDLKYVVGYSSVSHM
GYVLLGVASLQVIGVNGAVANMFAHGVMAALFFSMIGFIYEKTHTRWIPDLGGISHQAPRLSVGFMMAALASLGLPGLIS
FVPEFVIFVQSFKSFGLLAVVAIAGIIITALYVLRAGANTLFGPAREEYNHIKDIRGAELVPLVVLGSVLVIGGILPSLL
FDMVNSGVAPILAHINEALQLGGR
>Mature_503_residues
SIPVLTITLLSPMLAAFVIMFIPKEEEKLCKWVAAAGTGTALLLSLYVYFGYDQTLGGLQWVDFGGPIPWIRDLGVSYYM
AVDGLSLPMLLLTNLIGFSAVFASWNVTLRAKELMIYLLILIAGVMGTFIAQDLFIFLLFYEVVVIPIYIMVIIWGSSKR
VTKEYAGMKLTIYLLIGSAFLLVGVIATFVKSVAITGVPNMSFEALAQVAFPEQFQIYMFALMLIGFGSLLSMWPFHSWS
PDGYAGAPTAVSMIHAGVLKKIGGYGLIRFAVFVLPLGAKFWAPYIAVLGVIGVAYAAMGALAQKDLKYVVGYSSVSHMG
YVLLGVASLQVIGVNGAVANMFAHGVMAALFFSMIGFIYEKTHTRWIPDLGGISHQAPRLSVGFMMAALASLGLPGLISF
VPEFVIFVQSFKSFGLLAVVAIAGIIITALYVLRAGANTLFGPAREEYNHIKDIRGAELVPLVVLGSVLVIGGILPSLLF
DMVNSGVAPILAHINEALQLGGR

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=354, Percent_Identity=28.8135593220339, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788613, Length=492, Percent_Identity=32.520325203252, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1788827, Length=409, Percent_Identity=23.960880195599, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788831, Length=280, Percent_Identity=27.8571428571429, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693160, Length=268, Percent_Identity=30.5970149253731, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788829, Length=429, Percent_Identity=24.009324009324, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788614, Length=490, Percent_Identity=25.5102040816327, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI2367154, Length=439, Percent_Identity=23.9179954441913, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54275; Mature: 54144

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIPVLTITLLSPMLAAFVIMFIPKEEEKLCKWVAAAGTGTALLLSLYVYFGYDQTLGGL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCCC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HHHHHCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]