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The map label for this gene is nuoN

Identifier: 134299882

GI number: 134299882

Start: 2235639

End: 2237069

Strand: Reverse

Name: nuoN

Synonym: Dred_2036

Alternate gene names: 134299882

Gene position: 2237069-2235639 (Counterclockwise)

Preceding gene: 134299883

Following gene: 134299881

Centisome position: 62.0

GC content: 44.23

Gene sequence:

>1431_bases
ATGGAATTTAATTTTGCCTTAATAACCACTGAACTGGCTTTGTTTATACTTGGTTTAGCCACATTTGTATTGGGGCTATT
GGTTCCCTCCCATTCCCGTAGAGGATTAGGAAGTTTTGCACTGCTGGGGCTGTTACTGGTACTAGGAATCACCATCATTA
ATTGGTCCAACCAAGGAGTCCTGTTAGACGGTATGTATTTGGTAGACCAATATGCTACCTTTTTTAAAATATTGTGTCTA
ATCTCTGCTATCCTAGTTGTCTTAGGTTCCTTCCGATATATAGACAAACAGGTTGGGAATCAATTTGAATATTATTCTAC
GGTTATTTTTACTACCCTTGGTATGGTGGTTATGGCTTCAGCCGGTGACTTTATTACGCTGTATCTTGGACTTGAATTAA
TGACCATTTCATTTGTTATTCTGGTGGCCTTTCGATCCTTTGAGGCGAAATCCTTAGAGGCAGGGATGAAGTATATTCTG
TTGGCTGGCCTATCATCGGCGGTATTGCTTTACGGCCTAAGCTTGGTCTATGGTGCCACAGGAACGATCCTCATTGGTGA
AGTGGCCCGAGAAATTGCTGACAAAGGAAGTACACCCCTGTTGATTGTGGGGGTGGTTATGTTGGTGGCCGGACTGGGCT
TTAAAATATCGGCTGTTCCCTTTCATATGTGGTCGCCGGATGTTTATGAAGGCGCTCCCACCCCGGTTACCTCTTTTCTG
GCCGTCGGTTCTAAGGCAGCTTCCTTTGCGGTTTTACTAAGGTTATTTGCGGGCGGCTTTGGCGGTATACAAGAGCAGTG
GACTTTACTGGTGGCAGTCCTGGCTGCACTAAGCATGTTGATTGGCAACCTTGTGGCCATTCCGCAAACTAATATTAAAA
GGATGCTTGCTTACTCCAGTATTGCTCAGGCAGGCTATATAATGGTTGGATTGGTATCTGCCACGGAAGCAGGTATTAAG
GGTGTTATGTTTTATGCTTTTCTCTATGTTTTTGCGACCATCGGTGCTTTTACGGTAGTGGCTATCGTTAGTAATCAAAC
CAACAGTGATGAAATTAGAGATTATGCAGGTCTCATCCAAAGGGCTCCTCTGGCAGCCAGTGTGATGCTGATCTGTCTCC
TATCCATGGCTGGGATTCCACCCTTGGCTGGCTTTGTGGGCAAATTCTACCTTTTCAAAACAGTGGTCGAGAACCATATG
TGGCTTGTATATTTGGGTCTAATTATGAGTATGGTGTCGGTATATTATTACTTAAGAGTTGCCCTAGTGATGTTTAGGGA
TGAACCTAAGGAATCGGCTCCTATTCATGTGGGAGGTGCTGCAACCATAACCCTGGTAATTACGATGGTAGCTACCATTA
TTTTAGGAATTTACCCGGGTCCATTGGCGGAAGTTGCTAATATGGCTGCTCAATCCTTCCTGTTAAAATAG

Upstream 100 bases:

>100_bases
GAGGAATACTTCCCTCACTGCTGTTTGATATGGTGAATAGCGGGGTAGCGCCTATTCTGGCACATATTAATGAAGCCCTG
CAGTTAGGGGGGAGATAAAA

Downstream 100 bases:

>100_bases
TTTTTTAGTAAATAAAGTTGTCTGTTTGTTAGCTAAGTTGTTGCTCTATGTAGACCTCAAATCATAAGTCTACATAGAGC
TTTTTTAGATATAGAAAAGT

Product: proton-translocating NADH-quinone oxidoreductase, chain N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MEFNFALITTELALFILGLATFVLGLLVPSHSRRGLGSFALLGLLLVLGITIINWSNQGVLLDGMYLVDQYATFFKILCL
ISAILVVLGSFRYIDKQVGNQFEYYSTVIFTTLGMVVMASAGDFITLYLGLELMTISFVILVAFRSFEAKSLEAGMKYIL
LAGLSSAVLLYGLSLVYGATGTILIGEVAREIADKGSTPLLIVGVVMLVAGLGFKISAVPFHMWSPDVYEGAPTPVTSFL
AVGSKAASFAVLLRLFAGGFGGIQEQWTLLVAVLAALSMLIGNLVAIPQTNIKRMLAYSSIAQAGYIMVGLVSATEAGIK
GVMFYAFLYVFATIGAFTVVAIVSNQTNSDEIRDYAGLIQRAPLAASVMLICLLSMAGIPPLAGFVGKFYLFKTVVENHM
WLVYLGLIMSMVSVYYYLRVALVMFRDEPKESAPIHVGGAATITLVITMVATIILGIYPGPLAEVANMAAQSFLLK

Sequences:

>Translated_476_residues
MEFNFALITTELALFILGLATFVLGLLVPSHSRRGLGSFALLGLLLVLGITIINWSNQGVLLDGMYLVDQYATFFKILCL
ISAILVVLGSFRYIDKQVGNQFEYYSTVIFTTLGMVVMASAGDFITLYLGLELMTISFVILVAFRSFEAKSLEAGMKYIL
LAGLSSAVLLYGLSLVYGATGTILIGEVAREIADKGSTPLLIVGVVMLVAGLGFKISAVPFHMWSPDVYEGAPTPVTSFL
AVGSKAASFAVLLRLFAGGFGGIQEQWTLLVAVLAALSMLIGNLVAIPQTNIKRMLAYSSIAQAGYIMVGLVSATEAGIK
GVMFYAFLYVFATIGAFTVVAIVSNQTNSDEIRDYAGLIQRAPLAASVMLICLLSMAGIPPLAGFVGKFYLFKTVVENHM
WLVYLGLIMSMVSVYYYLRVALVMFRDEPKESAPIHVGGAATITLVITMVATIILGIYPGPLAEVANMAAQSFLLK
>Mature_476_residues
MEFNFALITTELALFILGLATFVLGLLVPSHSRRGLGSFALLGLLLVLGITIINWSNQGVLLDGMYLVDQYATFFKILCL
ISAILVVLGSFRYIDKQVGNQFEYYSTVIFTTLGMVVMASAGDFITLYLGLELMTISFVILVAFRSFEAKSLEAGMKYIL
LAGLSSAVLLYGLSLVYGATGTILIGEVAREIADKGSTPLLIVGVVMLVAGLGFKISAVPFHMWSPDVYEGAPTPVTSFL
AVGSKAASFAVLLRLFAGGFGGIQEQWTLLVAVLAALSMLIGNLVAIPQTNIKRMLAYSSIAQAGYIMVGLVSATEAGIK
GVMFYAFLYVFATIGAFTVVAIVSNQTNSDEIRDYAGLIQRAPLAASVMLICLLSMAGIPPLAGFVGKFYLFKTVVENHM
WLVYLGLIMSMVSVYYYLRVALVMFRDEPKESAPIHVGGAATITLVITMVATIILGIYPGPLAEVANMAAQSFLLK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=392, Percent_Identity=23.7244897959184, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI251831108, Length=337, Percent_Identity=21.9584569732938, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI145693160, Length=413, Percent_Identity=36.5617433414044, Blast_Score=267, Evalue=9e-73,
Organism=Escherichia coli, GI1788614, Length=378, Percent_Identity=25.3968253968254, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788827, Length=309, Percent_Identity=26.537216828479, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788831, Length=377, Percent_Identity=24.4031830238727, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788829, Length=325, Percent_Identity=24.9230769230769, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788613, Length=333, Percent_Identity=23.7237237237237, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_DESRM (A4J650)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113378.1
- STRING:   A4J650
- GeneID:   4957485
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_2036
- HOGENOM:   HBG747830
- OMA:   MDMNTLL
- PhylomeDB:   A4J650
- ProtClustDB:   CLSK2787191
- BioCyc:   DRED349161:DRED_2036-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 51061; Mature: 51061

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xfac3570)-; HASH(0xce12b00)-; HASH(0xcd912d4)-; HASH(0xfa5b100)-; HASH(0xf23f224)-; HASH(0xfdaaf1c)-; HASH(0xde3368c)-; HASH(0xf0e16fc)-; HASH(0xf8aba7c)-; HASH(0xfddf468)-; HASH(0xfaa30f4)-; HASH(0xfabd488)-; HASH(0xddc445c)-; HASH(0xef3c3b0)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNFALITTELALFILGLATFVLGLLVPSHSRRGLGSFALLGLLLVLGITIINWSNQGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCE
LLDGMYLVDQYATFFKILCLISAILVVLGSFRYIDKQVGNQFEYYSTVIFTTLGMVVMAS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
AGDFITLYLGLELMTISFVILVAFRSFEAKSLEAGMKYILLAGLSSAVLLYGLSLVYGAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTILIGEVAREIADKGSTPLLIVGVVMLVAGLGFKISAVPFHMWSPDVYEGAPTPVTSFL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECEEECCCCCCCCCCCCCHHHHH
AVGSKAASFAVLLRLFAGGFGGIQEQWTLLVAVLAALSMLIGNLVAIPQTNIKRMLAYSS
HHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IAQAGYIMVGLVSATEAGIKGVMFYAFLYVFATIGAFTVVAIVSNQTNSDEIRDYAGLIQ
HHHCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAPLAASVMLICLLSMAGIPPLAGFVGKFYLFKTVVENHMWLVYLGLIMSMVSVYYYLRV
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALVMFRDEPKESAPIHVGGAATITLVITMVATIILGIYPGPLAEVANMAAQSFLLK
HHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCH
>Mature Secondary Structure
MEFNFALITTELALFILGLATFVLGLLVPSHSRRGLGSFALLGLLLVLGITIINWSNQGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCE
LLDGMYLVDQYATFFKILCLISAILVVLGSFRYIDKQVGNQFEYYSTVIFTTLGMVVMAS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
AGDFITLYLGLELMTISFVILVAFRSFEAKSLEAGMKYILLAGLSSAVLLYGLSLVYGAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GTILIGEVAREIADKGSTPLLIVGVVMLVAGLGFKISAVPFHMWSPDVYEGAPTPVTSFL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECEEECCCCCCCCCCCCCHHHHH
AVGSKAASFAVLLRLFAGGFGGIQEQWTLLVAVLAALSMLIGNLVAIPQTNIKRMLAYSS
HHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IAQAGYIMVGLVSATEAGIKGVMFYAFLYVFATIGAFTVVAIVSNQTNSDEIRDYAGLIQ
HHHCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAPLAASVMLICLLSMAGIPPLAGFVGKFYLFKTVVENHMWLVYLGLIMSMVSVYYYLRV
HCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
ALVMFRDEPKESAPIHVGGAATITLVITMVATIILGIYPGPLAEVANMAAQSFLLK
HHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA