Click here to switch to the map view.

The map label for this gene is tnaA [H]

Identifier: 134299864

GI number: 134299864

Start: 2216451

End: 2217839

Strand: Reverse

Name: tnaA [H]

Synonym: Dred_2018

Alternate gene names: 134299864

Gene position: 2217839-2216451 (Counterclockwise)

Preceding gene: 134299865

Following gene: 134299862

Centisome position: 61.47

GC content: 40.1

Gene sequence:

>1389_bases
ATGACAAAAGTTCAACCAAAAGCAGAACCATTTAGAATTAAAATGGTTGAGCCCATTAAAATGATTAGCAAGGAAGAAAG
ACAAAGTGCTCTGGAAAGGGCCGGATACAACCTATTTTTATTAAATAGTGATGAAGTGTATATTGACCTTTTAACAGATA
GTGGCACAGGTGCTATGAGTGATAATCAGTGGGCTGGGCTTATGTTGGGTGACGAAGCTTATTCAGGTAGTAGAAACTTT
CATAACTTAAAAAATGCTGTAAAAGATATCTTTGATTATAACTTTACTATTCCCACGCACCAGGGGCGTGGGGCAGAGCA
AGTATTATTCCCTGTTATTATTAAGAAGCCAGGAGATCTATTTATCAGCAACATGCACTTTGATACAACCAAAGGTCATG
TTGAAATGGCTGGTGGACGTGCTATCAATATGGTTATTGAGGACGCCTATGATACGGAAAAATTCCATCCTTTTAAAGGT
AATTTTGACCTGAATAAATTAGAAGCCACCATTAAGGAAAAAGGTGCTGAAAACATTGCATGCGTAATTATTACAGTTAC
TTGTAATAGTTCGGGCGGGCAACCGGTATCGATGGAAAATATACGTCAGACCAGTGATCTTGCTAAAAGACATGGGATAC
GTGTGTTTATTGATGCGGCGAGATCCTACGAAAATGCTTACTTTATTAAGAGTAGGGAAGAAGGATATGCAGCCAAATCT
ATCCCTGAAATTGTTAAAGAGATGTACAGCTATGCTGATGGCTTTACAATGAGTGCTAAGAAGGATGCCATTGTAAATAT
AGGTGGTATTCTTGCCATCAAAGATGATGAAGATCTGTTTAACAAATGTGCAGCAAGAACGGTTCCGATGGAAGGATTCA
TTACCTATGGTGGTTTGGCAGGAAGAGATATGGAAGCTTTAGCTAGGGGCCTTTATGAAGGTATGGATTACGACTATCTG
GATTATCGGATTGGCCAAGTTGCCTATTTGGGTGAACGACTAAGGGAGGGGGGCGTCCCCATTCAGTTTCCTACGGGCGG
CCATGCAGTATTTGTTGATGCAAAGAAAATACTGCCTCACATCCCTTATTATCAGTTCCCTGCACAATCCCTTGCCAATG
AACTTTACTTGGATACTGGGGTCCGTAGTGTTGAAATTGGCTCTTTCCTGCTGGGTAGAGATCCTGATACTGGTGAAAAT
CTTGAATCTCCGATGGAACTACTAAGACTAACCATACCCAGAAGAACCTATACCTATAACCACATGGATATTATAGCCGA
AGGACTTATAAGTGTTAGCAAGAAGGCTAATGCTTTAAAAGGATTAGAATTTACCTATGAACCACCAATCCTAAGACACT
TTACCGCAAAGTTAAAACCTGTTCAGTAA

Upstream 100 bases:

>100_bases
TGGGTCAAAATACCATTTACCATATGGATTAAAAACATCTTGAACTGAAATAATAAGCAAAGAATAAACAAAAAACTGCT
TTTAAAATGGAGGTTTTTCT

Downstream 100 bases:

>100_bases
CCTATAAATAATTACGGAGGTAAAAACTATGGCAGAAAAAATTAAAAAGCCTACTAAAGAAACAAAGAATGAGATGAGGT
TGGAGCTAAATGTTGCATCC

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTKVQPKAEPFRIKMVEPIKMISKEERQSALERAGYNLFLLNSDEVYIDLLTDSGTGAMSDNQWAGLMLGDEAYSGSRNF
HNLKNAVKDIFDYNFTIPTHQGRGAEQVLFPVIIKKPGDLFISNMHFDTTKGHVEMAGGRAINMVIEDAYDTEKFHPFKG
NFDLNKLEATIKEKGAENIACVIITVTCNSSGGQPVSMENIRQTSDLAKRHGIRVFIDAARSYENAYFIKSREEGYAAKS
IPEIVKEMYSYADGFTMSAKKDAIVNIGGILAIKDDEDLFNKCAARTVPMEGFITYGGLAGRDMEALARGLYEGMDYDYL
DYRIGQVAYLGERLREGGVPIQFPTGGHAVFVDAKKILPHIPYYQFPAQSLANELYLDTGVRSVEIGSFLLGRDPDTGEN
LESPMELLRLTIPRRTYTYNHMDIIAEGLISVSKKANALKGLEFTYEPPILRHFTAKLKPVQ

Sequences:

>Translated_462_residues
MTKVQPKAEPFRIKMVEPIKMISKEERQSALERAGYNLFLLNSDEVYIDLLTDSGTGAMSDNQWAGLMLGDEAYSGSRNF
HNLKNAVKDIFDYNFTIPTHQGRGAEQVLFPVIIKKPGDLFISNMHFDTTKGHVEMAGGRAINMVIEDAYDTEKFHPFKG
NFDLNKLEATIKEKGAENIACVIITVTCNSSGGQPVSMENIRQTSDLAKRHGIRVFIDAARSYENAYFIKSREEGYAAKS
IPEIVKEMYSYADGFTMSAKKDAIVNIGGILAIKDDEDLFNKCAARTVPMEGFITYGGLAGRDMEALARGLYEGMDYDYL
DYRIGQVAYLGERLREGGVPIQFPTGGHAVFVDAKKILPHIPYYQFPAQSLANELYLDTGVRSVEIGSFLLGRDPDTGEN
LESPMELLRLTIPRRTYTYNHMDIIAEGLISVSKKANALKGLEFTYEPPILRHFTAKLKPVQ
>Mature_461_residues
TKVQPKAEPFRIKMVEPIKMISKEERQSALERAGYNLFLLNSDEVYIDLLTDSGTGAMSDNQWAGLMLGDEAYSGSRNFH
NLKNAVKDIFDYNFTIPTHQGRGAEQVLFPVIIKKPGDLFISNMHFDTTKGHVEMAGGRAINMVIEDAYDTEKFHPFKGN
FDLNKLEATIKEKGAENIACVIITVTCNSSGGQPVSMENIRQTSDLAKRHGIRVFIDAARSYENAYFIKSREEGYAAKSI
PEIVKEMYSYADGFTMSAKKDAIVNIGGILAIKDDEDLFNKCAARTVPMEGFITYGGLAGRDMEALARGLYEGMDYDYLD
YRIGQVAYLGERLREGGVPIQFPTGGHAVFVDAKKILPHIPYYQFPAQSLANELYLDTGVRSVEIGSFLLGRDPDTGENL
ESPMELLRLTIPRRTYTYNHMDIIAEGLISVSKKANALKGLEFTYEPPILRHFTAKLKPVQ

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=464, Percent_Identity=54.3103448275862, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.1 [H]

Molecular weight: Translated: 51631; Mature: 51499

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVQPKAEPFRIKMVEPIKMISKEERQSALERAGYNLFLLNSDEVYIDLLTDSGTGAMS
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCC
DNQWAGLMLGDEAYSGSRNFHNLKNAVKDIFDYNFTIPTHQGRGAEQVLFPVIIKKPGDL
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCHHHEEEEEEEECCCCE
FISNMHFDTTKGHVEMAGGRAINMVIEDAYDTEKFHPFKGNFDLNKLEATIKEKGAENIA
EEEEEEECCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
CVIITVTCNSSGGQPVSMENIRQTSDLAKRHGIRVFIDAARSYENAYFIKSREEGYAAKS
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHCCCCEEEEEECCCCCHHHH
IPEIVKEMYSYADGFTMSAKKDAIVNIGGILAIKDDEDLFNKCAARTVPMEGFITYGGLA
HHHHHHHHHHHCCCCEECCCCCCEEEECEEEEEECCHHHHHHHHHCCCCCCCEEEECCCC
GRDMEALARGLYEGMDYDYLDYRIGQVAYLGERLREGGVPIQFPTGGHAVFVDAKKILPH
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEHHHHCCC
IPYYQFPAQSLANELYLDTGVRSVEIGSFLLGRDPDTGENLESPMELLRLTIPRRTYTYN
CCCCCCCHHHHHHHHHHHCCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEC
HMDIIAEGLISVSKKANALKGLEFTYEPPILRHFTAKLKPVQ
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TKVQPKAEPFRIKMVEPIKMISKEERQSALERAGYNLFLLNSDEVYIDLLTDSGTGAMS
CCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCC
DNQWAGLMLGDEAYSGSRNFHNLKNAVKDIFDYNFTIPTHQGRGAEQVLFPVIIKKPGDL
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCHHHEEEEEEEECCCCE
FISNMHFDTTKGHVEMAGGRAINMVIEDAYDTEKFHPFKGNFDLNKLEATIKEKGAENIA
EEEEEEECCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
CVIITVTCNSSGGQPVSMENIRQTSDLAKRHGIRVFIDAARSYENAYFIKSREEGYAAKS
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEHHCCCCEEEEEECCCCCHHHH
IPEIVKEMYSYADGFTMSAKKDAIVNIGGILAIKDDEDLFNKCAARTVPMEGFITYGGLA
HHHHHHHHHHHCCCCEECCCCCCEEEECEEEEEECCHHHHHHHHHCCCCCCCEEEECCCC
GRDMEALARGLYEGMDYDYLDYRIGQVAYLGERLREGGVPIQFPTGGHAVFVDAKKILPH
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEHHHHCCC
IPYYQFPAQSLANELYLDTGVRSVEIGSFLLGRDPDTGENLESPMELLRLTIPRRTYTYN
CCCCCCCHHHHHHHHHHHCCCCEEEECCEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEC
HMDIIAEGLISVSKKANALKGLEFTYEPPILRHFTAKLKPVQ
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA