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The map label for this gene is topA [H]

Identifier: 134299831

GI number: 134299831

Start: 2178161

End: 2180293

Strand: Reverse

Name: topA [H]

Synonym: Dred_1984

Alternate gene names: 134299831

Gene position: 2180293-2178161 (Counterclockwise)

Preceding gene: 134299832

Following gene: 134299830

Centisome position: 60.43

GC content: 42.24

Gene sequence:

>2133_bases
TTGAGTAAAACATTAGTAATTGTTGAATCACCTGCTAAAGCAAAAAGCATTGGCAAATATTTAGGCAAAAACTATACAGT
AAAAGCTTCCATGGGACATGTGAGAGATTTACCCAAAAGCCAGTTTGGTGTTGATGTTGAAAATGCATTTTCTCCAAAAT
ACATAACAATTCGCGGGAAAGGGGACATCCTTAAAGAACTCCGTTCTGCCGTTAAGAAAGTTGATCATGTTCTGCTGGCA
TCTGACCCGGACCGTGAAGGTGAGGCAATTGCCTGGCATTTAACCAAATTGTTGGAGATAGATGAAGAAACCCCTTGTCG
CATTGAATTTAATGAAATTACTAAAAATGCCATCCAAGCTGCTGTTAAGCAACCACGGCGCATTGACGTGGATAGGGTTA
ATGCACAGCAGGCAAGAAGAATATTAGACAGGCTGGTGGGTTATAATTTAAGCCCTCTCTTGTGGCGAAAGGTTAAAAAA
GGGCTATCTGCAGGTCGAGTTCAGTCAGTGGCTGTACGCCTAATTTGTGATCGGGAAGAAGAAATACAGGCGTTTATTCC
AGAGGAATACTGGACTCTGACGGGAAAATTCTTAAAATCAGCAAAGTCTCCCTTTGAGGCTAAACTGCATAAACACAAGA
ATAAAAAAATAGTAATACCAAATCAAGAGACTATGGATAAAATCCTAGCAGAATTAAAGGGAGCAGCATACGTTGTTTCA
AACGTAACCAGAAAAGAGAGATTGCGTAACCCAGCACCTCCCTTTACCACCAGTTCCTTGCAGCAGGAAGCTTCGAGAAA
GCTTAATTTTACGTCCCGTAAGACCATGGTTGTGGCACAACAGCTTTATGAAGGTATCGATTTGGGCAAAGAAGGACCCG
TAGGTTTAGTGACTTATATACGTACCGACTCCACTAGAGTATCTGAAACCGCTATTGAAGAAACCCGAAGTTTTATTATG
GAGCGGTTTGGCCCAGCCTATGTACCAAAGCAACCAAGACAGGTTGTAGCCAAAGGAAAAGTGCAGGATGCCCACGAGGC
CATTCGACCCACATCCGTTAACCGTGACCCAGATAGTATTAAACAATACTTAACCAATGATCAATTCAAACTTTATAAAT
TAATATGGTCTCGATTTTTGGCCAGTCAAATGGCATCGGCGGTTATTGACACCACGTCCATGGATATAAGTGCAGGGGAT
TACCTATTCCGGGCCACAGGATCGATTATTAAATTTCCAGGATTTATGCAAGTTTACATAGAGGGTAACGATGATGGCCA
TAAAGAAGAAGAGAGTGTTTTGCCTGAATTAGCTAAGGGGGATAAGGTGCAGGCCAAAGACTTAACGCCTAAGCAACATT
TTACACAACCTCCCCCACGCTATACGGACGCTACTCTGGTAAAGGTGTTGGAAGAGAAAGGCATTGGACGTCCAAGTACC
TATGCACCTATCGTTGAGACCATTCAAAGACGCGGCTACGTTGTAAGAGAAAACAAACAGTTTTATCCCACCGAATTGGG
CGTCATTGTTGTTGATTTATTGAAAAACCATTTTCCAGAGATTATTAATGTAGAATTCACGGCGGATATGGAGAAAAAAC
TTGATCAGATTGCTGAGGGGGAACAACAGTGGGCGGGTGTTCTAAGGGATTTTTATGAACCCTTTTCCCAAACCCTGGAA
ACAGCTGAAGAAGCAATTGGTAAAGTGCAAGTGGCAGATGAGGTTTCGGAGGAAATTTGCGAACAATGCGGTAGAAATAT
GGTTATTAAACTTGGGCGATACGGTAAATTTCTTGCTTGCCCAGGGTTTCCGGACTGTCGAAATACAAAACCCCTCTTAG
AACCAACCGGTGTTGGCTGCCCTAAGTGTGAGGGTGAAATGGTATTAAGACGAAGCAAGAAAGGACGCAAATTTTATGGA
TGCAGCAGATACCCGGAATGCGAATTTGTAACATGGGATATGCCGACCAAGGAAAAATGCACCCAATGTGGTGAAATGAT
GGTGGAGAAAAAATCCCGGGGCAAAGAAAAGGTTTTGCAATGTGTAAATGAACATTGTCCCAGTAAAGGAGAAAAGGCTG
CATCCAATATAAACGGAAATAAAAAGAAGACAGTGAAAAACAATAAGAAGTAA

Upstream 100 bases:

>100_bases
TATTGATACAGTAAAAGTAGTTAGGGGCCAGTAAAGTTAACGAGAATCAGCTCCTTACAGAGGAGCGTTGAAAAGGCTTT
TCTAAACGAGGTGTACCAAT

Downstream 100 bases:

>100_bases
AAGGTTTAAGAGTGAGAAGGGCTGATAAACAGTATTCTTCTCATTTTCTTATCCCCTATCAATCAACAGGAGGACTATTC
TACATGACAGAGCACAGGGT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 710; Mature: 709

Protein sequence:

>710_residues
MSKTLVIVESPAKAKSIGKYLGKNYTVKASMGHVRDLPKSQFGVDVENAFSPKYITIRGKGDILKELRSAVKKVDHVLLA
SDPDREGEAIAWHLTKLLEIDEETPCRIEFNEITKNAIQAAVKQPRRIDVDRVNAQQARRILDRLVGYNLSPLLWRKVKK
GLSAGRVQSVAVRLICDREEEIQAFIPEEYWTLTGKFLKSAKSPFEAKLHKHKNKKIVIPNQETMDKILAELKGAAYVVS
NVTRKERLRNPAPPFTTSSLQQEASRKLNFTSRKTMVVAQQLYEGIDLGKEGPVGLVTYIRTDSTRVSETAIEETRSFIM
ERFGPAYVPKQPRQVVAKGKVQDAHEAIRPTSVNRDPDSIKQYLTNDQFKLYKLIWSRFLASQMASAVIDTTSMDISAGD
YLFRATGSIIKFPGFMQVYIEGNDDGHKEEESVLPELAKGDKVQAKDLTPKQHFTQPPPRYTDATLVKVLEEKGIGRPST
YAPIVETIQRRGYVVRENKQFYPTELGVIVVDLLKNHFPEIINVEFTADMEKKLDQIAEGEQQWAGVLRDFYEPFSQTLE
TAEEAIGKVQVADEVSEEICEQCGRNMVIKLGRYGKFLACPGFPDCRNTKPLLEPTGVGCPKCEGEMVLRRSKKGRKFYG
CSRYPECEFVTWDMPTKEKCTQCGEMMVEKKSRGKEKVLQCVNEHCPSKGEKAASNINGNKKKTVKNNKK

Sequences:

>Translated_710_residues
MSKTLVIVESPAKAKSIGKYLGKNYTVKASMGHVRDLPKSQFGVDVENAFSPKYITIRGKGDILKELRSAVKKVDHVLLA
SDPDREGEAIAWHLTKLLEIDEETPCRIEFNEITKNAIQAAVKQPRRIDVDRVNAQQARRILDRLVGYNLSPLLWRKVKK
GLSAGRVQSVAVRLICDREEEIQAFIPEEYWTLTGKFLKSAKSPFEAKLHKHKNKKIVIPNQETMDKILAELKGAAYVVS
NVTRKERLRNPAPPFTTSSLQQEASRKLNFTSRKTMVVAQQLYEGIDLGKEGPVGLVTYIRTDSTRVSETAIEETRSFIM
ERFGPAYVPKQPRQVVAKGKVQDAHEAIRPTSVNRDPDSIKQYLTNDQFKLYKLIWSRFLASQMASAVIDTTSMDISAGD
YLFRATGSIIKFPGFMQVYIEGNDDGHKEEESVLPELAKGDKVQAKDLTPKQHFTQPPPRYTDATLVKVLEEKGIGRPST
YAPIVETIQRRGYVVRENKQFYPTELGVIVVDLLKNHFPEIINVEFTADMEKKLDQIAEGEQQWAGVLRDFYEPFSQTLE
TAEEAIGKVQVADEVSEEICEQCGRNMVIKLGRYGKFLACPGFPDCRNTKPLLEPTGVGCPKCEGEMVLRRSKKGRKFYG
CSRYPECEFVTWDMPTKEKCTQCGEMMVEKKSRGKEKVLQCVNEHCPSKGEKAASNINGNKKKTVKNNKK
>Mature_709_residues
SKTLVIVESPAKAKSIGKYLGKNYTVKASMGHVRDLPKSQFGVDVENAFSPKYITIRGKGDILKELRSAVKKVDHVLLAS
DPDREGEAIAWHLTKLLEIDEETPCRIEFNEITKNAIQAAVKQPRRIDVDRVNAQQARRILDRLVGYNLSPLLWRKVKKG
LSAGRVQSVAVRLICDREEEIQAFIPEEYWTLTGKFLKSAKSPFEAKLHKHKNKKIVIPNQETMDKILAELKGAAYVVSN
VTRKERLRNPAPPFTTSSLQQEASRKLNFTSRKTMVVAQQLYEGIDLGKEGPVGLVTYIRTDSTRVSETAIEETRSFIME
RFGPAYVPKQPRQVVAKGKVQDAHEAIRPTSVNRDPDSIKQYLTNDQFKLYKLIWSRFLASQMASAVIDTTSMDISAGDY
LFRATGSIIKFPGFMQVYIEGNDDGHKEEESVLPELAKGDKVQAKDLTPKQHFTQPPPRYTDATLVKVLEEKGIGRPSTY
APIVETIQRRGYVVRENKQFYPTELGVIVVDLLKNHFPEIINVEFTADMEKKLDQIAEGEQQWAGVLRDFYEPFSQTLET
AEEAIGKVQVADEVSEEICEQCGRNMVIKLGRYGKFLACPGFPDCRNTKPLLEPTGVGCPKCEGEMVLRRSKKGRKFYGC
SRYPECEFVTWDMPTKEKCTQCGEMMVEKKSRGKEKVLQCVNEHCPSKGEKAASNINGNKKKTVKNNKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=619, Percent_Identity=25.3634894991922, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI4507635, Length=569, Percent_Identity=22.3198594024605, Blast_Score=117, Evalue=3e-26,
Organism=Escherichia coli, GI1787529, Length=752, Percent_Identity=41.2234042553192, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1788061, Length=538, Percent_Identity=23.0483271375465, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI48994931, Length=99, Percent_Identity=38.3838383838384, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17555378, Length=530, Percent_Identity=24.7169811320755, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=579, Percent_Identity=25.0431778929188, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=559, Percent_Identity=22.7191413237925, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585251, Length=667, Percent_Identity=23.5382308845577, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640096, Length=576, Percent_Identity=23.0902777777778, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80209; Mature: 80078

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTLVIVESPAKAKSIGKYLGKNYTVKASMGHVRDLPKSQFGVDVENAFSPKYITIRGK
CCCEEEEEECCHHHHHHHHHHCCCEEEEECCCHHHCCCHHHCCCCHHCCCCCCEEEEECC
GDILKELRSAVKKVDHVLLASDPDREGEAIAWHLTKLLEIDEETPCRIEFNEITKNAIQA
HHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
AVKQPRRIDVDRVNAQQARRILDRLVGYNLSPLLWRKVKKGLSAGRVQSVAVRLICDREE
HHCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
EIQAFIPEEYWTLTGKFLKSAKSPFEAKLHKHKNKKIVIPNQETMDKILAELKGAAYVVS
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCHHHHHH
NVTRKERLRNPAPPFTTSSLQQEASRKLNFTSRKTMVVAQQLYEGIDLGKEGPVGLVTYI
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
RTDSTRVSETAIEETRSFIMERFGPAYVPKQPRQVVAKGKVQDAHEAIRPTSVNRDPDSI
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCHHHH
KQYLTNDQFKLYKLIWSRFLASQMASAVIDTTSMDISAGDYLFRATGSIIKFPGFMQVYI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCEEECCCEEEEEE
EGNDDGHKEEESVLPELAKGDKVQAKDLTPKQHFTQPPPRYTDATLVKVLEEKGIGRPST
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCC
YAPIVETIQRRGYVVRENKQFYPTELGVIVVDLLKNHFPEIINVEFTADMEKKLDQIAEG
HHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCC
EQQWAGVLRDFYEPFSQTLETAEEAIGKVQVADEVSEEICEQCGRNMVIKLGRYGKFLAC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEC
PGFPDCRNTKPLLEPTGVGCPKCEGEMVLRRSKKGRKFYGCSRYPECEFVTWDMPTKEKC
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCEEECCCCCHHHH
TQCGEMMVEKKSRGKEKVLQCVNEHCPSKGEKAASNINGNKKKTVKNNKK
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTLVIVESPAKAKSIGKYLGKNYTVKASMGHVRDLPKSQFGVDVENAFSPKYITIRGK
CCEEEEEECCHHHHHHHHHHCCCEEEEECCCHHHCCCHHHCCCCHHCCCCCCEEEEECC
GDILKELRSAVKKVDHVLLASDPDREGEAIAWHLTKLLEIDEETPCRIEFNEITKNAIQA
HHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHH
AVKQPRRIDVDRVNAQQARRILDRLVGYNLSPLLWRKVKKGLSAGRVQSVAVRLICDREE
HHCCCCCCCHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
EIQAFIPEEYWTLTGKFLKSAKSPFEAKLHKHKNKKIVIPNQETMDKILAELKGAAYVVS
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCHHHHHH
NVTRKERLRNPAPPFTTSSLQQEASRKLNFTSRKTMVVAQQLYEGIDLGKEGPVGLVTYI
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
RTDSTRVSETAIEETRSFIMERFGPAYVPKQPRQVVAKGKVQDAHEAIRPTSVNRDPDSI
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCHHHH
KQYLTNDQFKLYKLIWSRFLASQMASAVIDTTSMDISAGDYLFRATGSIIKFPGFMQVYI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCEEECCCEEEEEE
EGNDDGHKEEESVLPELAKGDKVQAKDLTPKQHFTQPPPRYTDATLVKVLEEKGIGRPST
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCC
YAPIVETIQRRGYVVRENKQFYPTELGVIVVDLLKNHFPEIINVEFTADMEKKLDQIAEG
HHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCC
EQQWAGVLRDFYEPFSQTLETAEEAIGKVQVADEVSEEICEQCGRNMVIKLGRYGKFLAC
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEC
PGFPDCRNTKPLLEPTGVGCPKCEGEMVLRRSKKGRKFYGCSRYPECEFVTWDMPTKEKC
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCCCCCCEEECCCCCHHHH
TQCGEMMVEKKSRGKEKVLQCVNEHCPSKGEKAASNINGNKKKTVKNNKK
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]