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The map label for this gene is trmFO

Identifier: 134299830

GI number: 134299830

Start: 2176752

End: 2178077

Strand: Reverse

Name: trmFO

Synonym: Dred_1983

Alternate gene names: 134299830

Gene position: 2178077-2176752 (Counterclockwise)

Preceding gene: 134299831

Following gene: 134299829

Centisome position: 60.37

GC content: 43.51

Gene sequence:

>1326_bases
ATGACAGAGCACAGGGTTACAGTTATTGGTGCGGGATTGGCTGGTTCGGAGGCTGCATGGCAATTAGCTCGTAGGGGTAT
ACATGTGGACCTCTACGAGATGCGACCGCAGAAATACCCTCCGGCCCACCATACAGCCTATTTTTCAGAACTGGTTTGTA
GTAATTCCTTAAGGGCTGCTGCCATAGAAAATGCAGTAGGTTTACTAAAAGAGGAAATGAGGCAGCTTGACTCTTTAATT
ATCTCAGCCGCAGATAATCACAAGGTTCCGGCTGGTGGGGCACTGGCAGTTGACCGGGAAGGTTTTTCTCGATATATTAC
AGAGACACTGGAAGATCATCCCTTGATAGATATACACAGGGAAGAAGTAACAAAGATACCTGAAAAGGGAATAGTAATTG
TGGCCTCAGGTCCCCTTACCTCCGAATCTTTATCTCATGAAATAGCTAAAGTTACAGGTGAACAGTATCTATATTTTTAT
GACGCAGCCGCTCCCATTGTGACCCTGGAGTCCCTTGATATGACAAAGGTTTTCCGAGCTTCGCGTTATGGTAAGGGTGA
GGAAGCTTATTTAAACTGTCCCATGGATCAAGATGAATACGAAAAATTCTATGATGCACTGATTCATGCGGAAAGGGCCC
CAATAAAAGAATTTGAAAAACAGGTACATTTTGAAGGCTGTATGCCAGTTGAAGTATTAGCCTCCCGAGGGAAGGATACA
TTGCTCTACGGGCCATTAAAGCCAGTTGGTCTAACTGACCCAAGAACTGGCAAAAGACCCCACGGGGTTGTTCAGTTGAG
GCAGGATAATGCTGAGGGTACTCTTTTTAATCTGGTGGGTTTTCAGACAAATCTTAAATGGGGTGAGCAAAAAAGAGTTT
TTAGCTTTATCCCGGGTCTGGAAGATGCTGAATTTGTGCGATACGGTGTTATGCACAGAAATACATATATTACATCTCCA
GTTCTCTTACAACCTACCCTTCAAATGAAAGAACACCCAAGAATATTCTTTGCCGGGCAAATTACCGGAGTAGAAGGTTA
TGTAGAATCTGCCGCAGCAGGATTAATAGCAGGAATCAACGCTGCAGCTTTGGCAAAACAGAAAGATTTATTAATTTTCC
CGAAAGAAACCGCTCATGGGGCATTAATGCATTATATAACCACTGCTTCCACAAAGAACTTTCAACCAATGAATGTTACC
TTTGGTCTGTTTCCAGAGCTTACTGTCCGGCTTAAAGGCAAGCGGGAAAGGGGTCGTGCCCAGGCAGAGCGAGCTCTTGA
AATATTGAAGGAGTGGATTGAAAAAGAAAAAATATGTGATATATAA

Upstream 100 bases:

>100_bases
AAAACAATAAGAAGTAAAAGGTTTAAGAGTGAGAAGGGCTGATAAACAGTATTCTTCTCATTTTCTTATCCCCTATCAAT
CAACAGGAGGACTATTCTAC

Downstream 100 bases:

>100_bases
GAGACAATAGGGGAATAACAATATAATAATTAAATTGTCAAAAGGTAGTTGCAATTTCAAAAGGGCTTGTGATATATTTT
ATTAGGTGTCATATTTCGAC

Product: gid protein

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTEHRVTVIGAGLAGSEAAWQLARRGIHVDLYEMRPQKYPPAHHTAYFSELVCSNSLRAAAIENAVGLLKEEMRQLDSLI
ISAADNHKVPAGGALAVDREGFSRYITETLEDHPLIDIHREEVTKIPEKGIVIVASGPLTSESLSHEIAKVTGEQYLYFY
DAAAPIVTLESLDMTKVFRASRYGKGEEAYLNCPMDQDEYEKFYDALIHAERAPIKEFEKQVHFEGCMPVEVLASRGKDT
LLYGPLKPVGLTDPRTGKRPHGVVQLRQDNAEGTLFNLVGFQTNLKWGEQKRVFSFIPGLEDAEFVRYGVMHRNTYITSP
VLLQPTLQMKEHPRIFFAGQITGVEGYVESAAAGLIAGINAAALAKQKDLLIFPKETAHGALMHYITTASTKNFQPMNVT
FGLFPELTVRLKGKRERGRAQAERALEILKEWIEKEKICDI

Sequences:

>Translated_441_residues
MTEHRVTVIGAGLAGSEAAWQLARRGIHVDLYEMRPQKYPPAHHTAYFSELVCSNSLRAAAIENAVGLLKEEMRQLDSLI
ISAADNHKVPAGGALAVDREGFSRYITETLEDHPLIDIHREEVTKIPEKGIVIVASGPLTSESLSHEIAKVTGEQYLYFY
DAAAPIVTLESLDMTKVFRASRYGKGEEAYLNCPMDQDEYEKFYDALIHAERAPIKEFEKQVHFEGCMPVEVLASRGKDT
LLYGPLKPVGLTDPRTGKRPHGVVQLRQDNAEGTLFNLVGFQTNLKWGEQKRVFSFIPGLEDAEFVRYGVMHRNTYITSP
VLLQPTLQMKEHPRIFFAGQITGVEGYVESAAAGLIAGINAAALAKQKDLLIFPKETAHGALMHYITTASTKNFQPMNVT
FGLFPELTVRLKGKRERGRAQAERALEILKEWIEKEKICDI
>Mature_440_residues
TEHRVTVIGAGLAGSEAAWQLARRGIHVDLYEMRPQKYPPAHHTAYFSELVCSNSLRAAAIENAVGLLKEEMRQLDSLII
SAADNHKVPAGGALAVDREGFSRYITETLEDHPLIDIHREEVTKIPEKGIVIVASGPLTSESLSHEIAKVTGEQYLYFYD
AAAPIVTLESLDMTKVFRASRYGKGEEAYLNCPMDQDEYEKFYDALIHAERAPIKEFEKQVHFEGCMPVEVLASRGKDTL
LYGPLKPVGLTDPRTGKRPHGVVQLRQDNAEGTLFNLVGFQTNLKWGEQKRVFSFIPGLEDAEFVRYGVMHRNTYITSPV
LLQPTLQMKEHPRIFFAGQITGVEGYVESAAAGLIAGINAAALAKQKDLLIFPKETAHGALMHYITTASTKNFQPMNVTF
GLFPELTVRLKGKRERGRAQAERALEILKEWIEKEKICDI

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=114, Percent_Identity=37.719298245614, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_DESRM (A4J5Z8)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113326.1
- ProteinModelPortal:   A4J5Z8
- STRING:   A4J5Z8
- GeneID:   4958581
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1983
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- BioCyc:   DRED349161:DRED_1983-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49237; Mature: 49106

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHRVTVIGAGLAGSEAAWQLARRGIHVDLYEMRPQKYPPAHHTAYFSELVCSNSLRAA
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHH
AIENAVGLLKEEMRQLDSLIISAADNHKVPAGGALAVDREGFSRYITETLEDHPLIDIHR
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEECH
EEVTKIPEKGIVIVASGPLTSESLSHEIAKVTGEQYLYFYDAAAPIVTLESLDMTKVFRA
HHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCHHHHHHH
SRYGKGEEAYLNCPMDQDEYEKFYDALIHAERAPIKEFEKQVHFEGCMPVEVLASRGKDT
HCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCE
LLYGPLKPVGLTDPRTGKRPHGVVQLRQDNAEGTLFNLVGFQTNLKWGEQKRVFSFIPGL
EEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECEECCCCCCHHHHHHHCCCC
EDAEFVRYGVMHRNTYITSPVLLQPTLQMKEHPRIFFAGQITGVEGYVESAAAGLIAGIN
CCHHHHHHHHHCCCEEECCCEEECCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCC
AAALAKQKDLLIFPKETAHGALMHYITTASTKNFQPMNVTFGLFPELTVRLKGKRERGRA
HHHHHCCCCEEEECCCCCCHHHHHHEECCCCCCCCCCEEEEECCCEEEEEECCCHHHHHH
QAERALEILKEWIEKEKICDI
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEHRVTVIGAGLAGSEAAWQLARRGIHVDLYEMRPQKYPPAHHTAYFSELVCSNSLRAA
CCCEEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHH
AIENAVGLLKEEMRQLDSLIISAADNHKVPAGGALAVDREGFSRYITETLEDHPLIDIHR
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEECH
EEVTKIPEKGIVIVASGPLTSESLSHEIAKVTGEQYLYFYDAAAPIVTLESLDMTKVFRA
HHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCCHHHHHHH
SRYGKGEEAYLNCPMDQDEYEKFYDALIHAERAPIKEFEKQVHFEGCMPVEVLASRGKDT
HCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCE
LLYGPLKPVGLTDPRTGKRPHGVVQLRQDNAEGTLFNLVGFQTNLKWGEQKRVFSFIPGL
EEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECEECCCCCCHHHHHHHCCCC
EDAEFVRYGVMHRNTYITSPVLLQPTLQMKEHPRIFFAGQITGVEGYVESAAAGLIAGIN
CCHHHHHHHHHCCCEEECCCEEECCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCC
AAALAKQKDLLIFPKETAHGALMHYITTASTKNFQPMNVTFGLFPELTVRLKGKRERGRA
HHHHHCCCCEEEECCCCCCHHHHHHEECCCCCCCCCCEEEEECCCEEEEEECCCHHHHHH
QAERALEILKEWIEKEKICDI
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA