Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 134299706

GI number: 134299706

Start: 2034269

End: 2036356

Strand: Reverse

Name: topB [H]

Synonym: Dred_1853

Alternate gene names: 134299706

Gene position: 2036356-2034269 (Counterclockwise)

Preceding gene: 134299708

Following gene: 134299705

Centisome position: 56.44

GC content: 41.43

Gene sequence:

>2088_bases
ATGAAACAGCTAATTCTTGCGGAAAAGCCATCAGTGGGGAGGGATATTGTAAATGCCATTGGTGGTCATTTTGAAAAAAG
GGATGGGTATTATGAAAATAGTCAGTATATTATTAGTTGGGCCATCGGGCATCTGGTTGAATTATGTGAACCAGAGGATT
ATCAACCTAACTTAAAAAAATGGACTCTCTCAGCTTTACCCATTATTCCAGAATGCTTCAAACTAAAACCCAACCCTAAA
ACAATTAAACAGTACAAAGTAGTTAAACAACTTCTCAACCGGCCTGATGTGGAACTAATTGCTGGGACGGATGCAGGGCG
TGAAGGAGAACTGATCTTAAGATATATTATGCGTTTATCAGGATACAGCAGGCCCTTTAAACGGCTCTGGTTGTCAGAGA
CAACACCAGAATCCATCAGAAAAGGACTGGCCAATCCGTTGCCAGGGCAGGCTAAGGATCTACTGGCTGCTGCAGCGGAG
TGCAGATCCCATGCAGACTGGTTAATTGGTATCAATGCTACTCGAGCCTTTACCAAAAAGCACGGAGATTTATTTTCAAT
AGGACGTGTACAAACTCCAACCTTAGGACTTATTGTAGCCAGAGAAAAAGAGATTAAAACGTTTATACCCGCCCATTACT
GGGAATTATATGCAGATTTTGTCACAACTGATGGTCAGGTTTATCGTGGCAAGTGGTTTAAAAATAAAAACAAAAACCGA
TTTAACCAGGAGGCAGACGGCATAAGCATCAAAAATAAAATTGTGGGACAAGATGGCTCGGTCATTAAGGTCGAGGAAAA
AGCAGTGTCTGAACCACCGCCCACCTTGTTTAACTTGAATGATTTACAAAAGGAAGCCAATAAAAAGTTTGGCTTTACTG
CAAGCCAGACCTTAGAAATAGCCCAGGCATTATATGAAAAACACAAACTATTAAGTTATCCTAGAACAGAGTTTAAACAC
CTTACGAACGAATTGGCTAAGACAATTCCTTCTCGGTTATCAGCCCTGGGGTCAGTGTCTGGCTATTCTAAGTTTGTGGC
CTACGCCCAAAGTGCCCCGAAACCGGGGAAGAGGTTCATCGATGACGCCAAGGTCGGAGATCACCATGCATTGATTCCGA
CAAATGTTAAGCCAACCGCCGCGCTGTCCCCCAATGAAGCTAAAATTTACAATATGGTGGCCCGTAGATTTTTAGCAATG
TTTTTTCCTGCGGCGGAATACAAGCAGACAAAAATCATGACTTCTGTGTCAGACGAAACATTTCTAACATCAGGCAAGAT
TGAACTAAGCCCAGGGTGGAAGGAAGTAATACCTCCATTAAAAGGTGAGAATAAGGAAAAAAGTGACGAGGAAGTCTTGC
TCCCTCCCTTAACGAAGGGGGAGAATGTTGCAGTAACCAAAGCTAAATTGCAACAGAAGAAAACGGAGCCTCCGAAAAGG
TATACAGAGGGAACACTACTGGCGGTTATGGAAGGGGCAGGGAAGTTTGTAGATGATAAGGCATTGAAGGATGCCATGAA
AGGACACGGACTGGGAACAAGTGCAACCAGGGCGGCAATAATTGAAAGATTAATTAAAGTGGGATACGTAGAGAGAAAGA
GCAAGACTTTAATACCAACCCAAAAGGGTGAAACACTTATTAGTTTGGTGCCAGAAGTGGTCTCTAGCCCTCAAATGACC
GGCGAATGGGAGAAAGCATTGGCAGATATAGAAGCAGGTCAAGTTAATCCCAAACGCTTTATGTCTGAGATTATTAGTCT
AACTAAAAAGATAGTTAACCTAGCTAAAGAACAAAAAACAAATGGATATATGCCCGAATCAACAAAGGAAGTAGTAGGAA
AATGTCCCTTATGTGGCAGAGAAGTTGTGGAATATGCAAAAAAATTTGGCTGTGGAGGATTCCGTGAGGGTTGTGCTTTT
TCATTTGGCAAACAAATTTTGAAGAAAAAAATAACAGTCAAACAGGCAAAACAGTTATTAACTAACGGTAAAGTACTGAT
TAAAGGATTCACATCTTCTAAGACAGGGAAAAAATTTGATGCGGCCTTAGTTCTTAAGGATGGAAAAGTTTCCTTTGATT
TTTCATAA

Upstream 100 bases:

>100_bases
GCATTGAAGGAGTCCTTTTAAGTTTTAGTGTATAATGCTAGATGAATGGATAGTAAATAGGTTTGATAGATATATGGCTT
TTTTCTACTGGGGGTTAAAA

Downstream 100 bases:

>100_bases
ATGTTAAGCTCACATATAATTCATTGAACAAAGAGACAATATCTATAGCAGTGAAAATAATCCTTACTTAGGGAGATTAT
CATGGTAACTTCGTTTGATA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MKQLILAEKPSVGRDIVNAIGGHFEKRDGYYENSQYIISWAIGHLVELCEPEDYQPNLKKWTLSALPIIPECFKLKPNPK
TIKQYKVVKQLLNRPDVELIAGTDAGREGELILRYIMRLSGYSRPFKRLWLSETTPESIRKGLANPLPGQAKDLLAAAAE
CRSHADWLIGINATRAFTKKHGDLFSIGRVQTPTLGLIVAREKEIKTFIPAHYWELYADFVTTDGQVYRGKWFKNKNKNR
FNQEADGISIKNKIVGQDGSVIKVEEKAVSEPPPTLFNLNDLQKEANKKFGFTASQTLEIAQALYEKHKLLSYPRTEFKH
LTNELAKTIPSRLSALGSVSGYSKFVAYAQSAPKPGKRFIDDAKVGDHHALIPTNVKPTAALSPNEAKIYNMVARRFLAM
FFPAAEYKQTKIMTSVSDETFLTSGKIELSPGWKEVIPPLKGENKEKSDEEVLLPPLTKGENVAVTKAKLQQKKTEPPKR
YTEGTLLAVMEGAGKFVDDKALKDAMKGHGLGTSATRAAIIERLIKVGYVERKSKTLIPTQKGETLISLVPEVVSSPQMT
GEWEKALADIEAGQVNPKRFMSEIISLTKKIVNLAKEQKTNGYMPESTKEVVGKCPLCGREVVEYAKKFGCGGFREGCAF
SFGKQILKKKITVKQAKQLLTNGKVLIKGFTSSKTGKKFDAALVLKDGKVSFDFS

Sequences:

>Translated_695_residues
MKQLILAEKPSVGRDIVNAIGGHFEKRDGYYENSQYIISWAIGHLVELCEPEDYQPNLKKWTLSALPIIPECFKLKPNPK
TIKQYKVVKQLLNRPDVELIAGTDAGREGELILRYIMRLSGYSRPFKRLWLSETTPESIRKGLANPLPGQAKDLLAAAAE
CRSHADWLIGINATRAFTKKHGDLFSIGRVQTPTLGLIVAREKEIKTFIPAHYWELYADFVTTDGQVYRGKWFKNKNKNR
FNQEADGISIKNKIVGQDGSVIKVEEKAVSEPPPTLFNLNDLQKEANKKFGFTASQTLEIAQALYEKHKLLSYPRTEFKH
LTNELAKTIPSRLSALGSVSGYSKFVAYAQSAPKPGKRFIDDAKVGDHHALIPTNVKPTAALSPNEAKIYNMVARRFLAM
FFPAAEYKQTKIMTSVSDETFLTSGKIELSPGWKEVIPPLKGENKEKSDEEVLLPPLTKGENVAVTKAKLQQKKTEPPKR
YTEGTLLAVMEGAGKFVDDKALKDAMKGHGLGTSATRAAIIERLIKVGYVERKSKTLIPTQKGETLISLVPEVVSSPQMT
GEWEKALADIEAGQVNPKRFMSEIISLTKKIVNLAKEQKTNGYMPESTKEVVGKCPLCGREVVEYAKKFGCGGFREGCAF
SFGKQILKKKITVKQAKQLLTNGKVLIKGFTSSKTGKKFDAALVLKDGKVSFDFS
>Mature_695_residues
MKQLILAEKPSVGRDIVNAIGGHFEKRDGYYENSQYIISWAIGHLVELCEPEDYQPNLKKWTLSALPIIPECFKLKPNPK
TIKQYKVVKQLLNRPDVELIAGTDAGREGELILRYIMRLSGYSRPFKRLWLSETTPESIRKGLANPLPGQAKDLLAAAAE
CRSHADWLIGINATRAFTKKHGDLFSIGRVQTPTLGLIVAREKEIKTFIPAHYWELYADFVTTDGQVYRGKWFKNKNKNR
FNQEADGISIKNKIVGQDGSVIKVEEKAVSEPPPTLFNLNDLQKEANKKFGFTASQTLEIAQALYEKHKLLSYPRTEFKH
LTNELAKTIPSRLSALGSVSGYSKFVAYAQSAPKPGKRFIDDAKVGDHHALIPTNVKPTAALSPNEAKIYNMVARRFLAM
FFPAAEYKQTKIMTSVSDETFLTSGKIELSPGWKEVIPPLKGENKEKSDEEVLLPPLTKGENVAVTKAKLQQKKTEPPKR
YTEGTLLAVMEGAGKFVDDKALKDAMKGHGLGTSATRAAIIERLIKVGYVERKSKTLIPTQKGETLISLVPEVVSSPQMT
GEWEKALADIEAGQVNPKRFMSEIISLTKKIVNLAKEQKTNGYMPESTKEVVGKCPLCGREVVEYAKKFGCGGFREGCAF
SFGKQILKKKITVKQAKQLLTNGKVLIKGFTSSKTGKKFDAALVLKDGKVSFDFS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=695, Percent_Identity=25.3237410071942, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI4507635, Length=637, Percent_Identity=24.6467817896389, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1788061, Length=614, Percent_Identity=32.4104234527687, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1787529, Length=568, Percent_Identity=25.1760563380282, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32563869, Length=620, Percent_Identity=26.7741935483871, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17555378, Length=652, Percent_Identity=24.6932515337423, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323263, Length=678, Percent_Identity=23.7463126843658, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585251, Length=703, Percent_Identity=25.74679943101, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640096, Length=523, Percent_Identity=26.3862332695985, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77302; Mature: 77302

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLILAEKPSVGRDIVNAIGGHFEKRDGYYENSQYIISWAIGHLVELCEPEDYQPNLKK
CCCCEECCCCCCCHHHHHHHCCCCHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCHHH
WTLSALPIIPECFKLKPNPKTIKQYKVVKQLLNRPDVELIAGTDAGREGELILRYIMRLS
EEHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHC
GYSRPFKRLWLSETTPESIRKGLANPLPGQAKDLLAAAAECRSHADWLIGINATRAFTKK
CCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHH
HGDLFSIGRVQTPTLGLIVAREKEIKTFIPAHYWELYADFVTTDGQVYRGKWFKNKNKNR
CCCEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
FNQEADGISIKNKIVGQDGSVIKVEEKAVSEPPPTLFNLNDLQKEANKKFGFTASQTLEI
CCCCCCCCCHHHHEECCCCCEEEEEHHHCCCCCCCEECHHHHHHHHCCCCCCCHHHHHHH
AQALYEKHKLLSYPRTEFKHLTNELAKTIPSRLSALGSVSGYSKFVAYAQSAPKPGKRFI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHH
DDAKVGDHHALIPTNVKPTAALSPNEAKIYNMVARRFLAMFFPAAEYKQTKIMTSVSDET
CCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
FLTSGKIELSPGWKEVIPPLKGENKEKSDEEVLLPPLTKGENVAVTKAKLQQKKTEPPKR
EEECCEEEECCCHHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEEHHHHHHHCCCCCCH
YTEGTLLAVMEGAGKFVDDKALKDAMKGHGLGTSATRAAIIERLIKVGYVERKSKTLIPT
HCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCC
QKGETLISLVPEVVSSPQMTGEWEKALADIEAGQVNPKRFMSEIISLTKKIVNLAKEQKT
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NGYMPESTKEVVGKCPLCGREVVEYAKKFGCGGFREGCAFSFGKQILKKKITVKQAKQLL
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TNGKVLIKGFTSSKTGKKFDAALVLKDGKVSFDFS
HCCCEEEEECCCCCCCCCCCEEEEEECCEEEECCC
>Mature Secondary Structure
MKQLILAEKPSVGRDIVNAIGGHFEKRDGYYENSQYIISWAIGHLVELCEPEDYQPNLKK
CCCCEECCCCCCCHHHHHHHCCCCHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCHHH
WTLSALPIIPECFKLKPNPKTIKQYKVVKQLLNRPDVELIAGTDAGREGELILRYIMRLS
EEHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHC
GYSRPFKRLWLSETTPESIRKGLANPLPGQAKDLLAAAAECRSHADWLIGINATRAFTKK
CCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHH
HGDLFSIGRVQTPTLGLIVAREKEIKTFIPAHYWELYADFVTTDGQVYRGKWFKNKNKNR
CCCEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
FNQEADGISIKNKIVGQDGSVIKVEEKAVSEPPPTLFNLNDLQKEANKKFGFTASQTLEI
CCCCCCCCCHHHHEECCCCCEEEEEHHHCCCCCCCEECHHHHHHHHCCCCCCCHHHHHHH
AQALYEKHKLLSYPRTEFKHLTNELAKTIPSRLSALGSVSGYSKFVAYAQSAPKPGKRFI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHH
DDAKVGDHHALIPTNVKPTAALSPNEAKIYNMVARRFLAMFFPAAEYKQTKIMTSVSDET
CCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
FLTSGKIELSPGWKEVIPPLKGENKEKSDEEVLLPPLTKGENVAVTKAKLQQKKTEPPKR
EEECCEEEECCCHHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEEHHHHHHHCCCCCCH
YTEGTLLAVMEGAGKFVDDKALKDAMKGHGLGTSATRAAIIERLIKVGYVERKSKTLIPT
HCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCC
QKGETLISLVPEVVSSPQMTGEWEKALADIEAGQVNPKRFMSEIISLTKKIVNLAKEQKT
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
NGYMPESTKEVVGKCPLCGREVVEYAKKFGCGGFREGCAFSFGKQILKKKITVKQAKQLL
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TNGKVLIKGFTSSKTGKKFDAALVLKDGKVSFDFS
HCCCEEEEECCCCCCCCCCCEEEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA