Click here to switch to the map view.

The map label for this gene is metE

Identifier: 134299605

GI number: 134299605

Start: 1914246

End: 1916540

Strand: Reverse

Name: metE

Synonym: Dred_1751

Alternate gene names: 134299605

Gene position: 1916540-1914246 (Counterclockwise)

Preceding gene: 134299606

Following gene: 134299604

Centisome position: 53.12

GC content: 36.34

Gene sequence:

>2295_bases
TTGTACACAGGAGCAACTACTTATGTGGTGGGTTTCCCGAGAATCGGAGAACAAAGAGAACTAAAAAAAGTCCTCGAGCG
TTATTGGGATCAAAAAACGAGTTTTAGTGAGATTGACGAGATTGCAAAAAACTTACGGCGAAGACACTGGTTGTACCAAA
AAGAGGCAGGTATCTCTTTTATTAGTAGTAATGATTTTAGTTTATATGATAACATGCTTGATACGGCAATAATGTTAAAT
GCAGTTCCTGAAAGATTTAGTAATATCCATGACAAGCAAGAGCTTTATTTCGCGATGGCTCGAGGAACGAATAATACTGT
TGCAATGGAAATGACTAAGTGGTTTAATACCAATTATCACTATATTGTCCCCGAACTTTCCGATACCATGAACTTTTCAT
TATGTGCAGATAAAATAATTAATGAGTATCGGGAAGCAAAAGAATATGGCCTTCGGACAAAAATTAACTTAATTGGGCCA
ATAACATTTTTGTCTCTTTCCAAGCCTGTTGACGGTAATCAAGATACCTTGGAACTTTTGCCAAAGATTTTGCCTTGTTA
TGTTTCTCTTCTCAAAAAAATTGCGGATCTTGATGATGAAGTATACATCCAATTTGACGAACCGATCGTTGTAAAGGATA
TCGACAGACGAACACTAAATCTGTTTTACTTATGCTATAAAGAATTATCCAACGTGAGTAACAGGCTTAGACTGATCGTA
ATGACTTATTTTGATCATGCGGTGGAGGCTGTAAAAATACTATCTGATATTCCAATTTACGGTATAGGCCTTGATTTTGT
TTATGGCCAGGAAAATCTTAAAGTCCTGGACTCCTTGAATGGAAAAAAACTGATTGCAGGTGTTATCGATGGAAGAAATA
TTTGGAAGAATAACTATCATAATACATTGACTTTACTAAAAGACATCGAAAAAAATGTTAAAAAAGAGGATATCATCCTT
TCCACGACCTGCTCTCTTCTACATGTCCCTTATTCTTTAAAGCATGAAAATCATCTGGACAAAGATATTAAATCCTGGAT
GAGTTTTGCTCGAGAAAAACTTGACGAACTATCGGATCTCGCAAAATTGTTTTTTGTTGAAAATAATTCAGAAGATATCC
TGACAATTCTTGAAAATAATCAAAGAATCTTTAGAGAAAAGCAACAATCTTCGAAAGTAATAGACCCTATAGTGCGAGAA
CGGGTAGGTAACATTAAAAGGAGAGAAAGAGATGGCTCTTTTAATGACAGAAATGAAGCACAGAGAAAAAAGTTAGACCT
ACCATTACTTCCAACAACAACCATTGGTAGCTTTCCTCAAACAGAAGAAATTAGAAAGCTACGACGGGATTTTAAAAATA
ATGTTATTTCTCAAATAGAATATGACACAGGAATTAAGAACTACATTGATTCATGTATTCAATTTCAAGAAGATATTGGG
CTGGATGTTTTGGTACATGGCGAACCAGAAAGAAATGATATGGTAGAGTATTTTGGCGAACGGCTTTCAGGTTTTGTTTT
TACGCAAAACGGCTGGGTACAAAGCTATGGCAGCAGATGCGTAAAGCCAACGGTTATCTTTGGTGATGTCAGTAGACCAA
AACCAATGACCATTGATACCATTTTGTATGCTAAAAGCAAAACCAATAAAATTGTAAAGGGAATGTTAACAGGACCTGTT
ACCATTCTTAATTGGTCCTATTGTCGAAGTGATATGGAACGTTCTGCTGTTTGTGAACAGATTGCGTTGGCCATTAGAGA
TGAGATTAATGACCTTCAGAAGGCAGGAATCAAAATTATTCAGGTTGATGAAGCCGCATTCAAAGAAGGATATCCTCTAC
GAAAAGAAAAAGTTGCATATTATGAAAATTGGGCTGTTAAATCTTTCAAATTAGCCGTTAGCTCTGCTGCCATAGAAACA
CAAATCCATACTCATATGTGTTACAGTGACTTTAATGATATTATTCATACCATAGAAAAGATGGACGCAGATGTTATTAC
CATTGAAACGTCCAGAAGCGGCAACAAACTGTTAACTGTTTTTGCAACTCGAGGGTATAAAAATGAGATTGGCCCTGGCA
TTTATGATATTCACTCGCCTAGAGTTCCATCTGTTGAAGAACTGGAAGAGAAGATTAGAAACTTAATGTTAGTTTTGCCA
CCGTCAAAACTGTGGATTAATCCTGACTGTGGCCTTAAAACTCGCAAGTGGGATGAGATAAGATGGAGTTTGTCTAATAT
GGTGAAGGCAGCACAGCACATTAGACTAGGATTGCAAAGGGTGATGTTTGTTTAG

Upstream 100 bases:

>100_bases
TCGCGCTACGGTAACGGAAGGCCTTAATGGCAAGTCCGGAGTCCGAATACCATGGTAATTGCTAATTTTTTGTCGCGTTA
ACAAAAAAGGAGGAGTTAAA

Downstream 100 bases:

>100_bases
ATGCAAGGCAAGGCAATGATGCCTTGCCTTGCATCTGTCTTTAACAGCAGACTAATTTAACTTTCTCATATTTTGATTTT
AAAAGGTGGATTAACATGAT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MYTGATTYVVGFPRIGEQRELKKVLERYWDQKTSFSEIDEIAKNLRRRHWLYQKEAGISFISSNDFSLYDNMLDTAIMLN
AVPERFSNIHDKQELYFAMARGTNNTVAMEMTKWFNTNYHYIVPELSDTMNFSLCADKIINEYREAKEYGLRTKINLIGP
ITFLSLSKPVDGNQDTLELLPKILPCYVSLLKKIADLDDEVYIQFDEPIVVKDIDRRTLNLFYLCYKELSNVSNRLRLIV
MTYFDHAVEAVKILSDIPIYGIGLDFVYGQENLKVLDSLNGKKLIAGVIDGRNIWKNNYHNTLTLLKDIEKNVKKEDIIL
STTCSLLHVPYSLKHENHLDKDIKSWMSFAREKLDELSDLAKLFFVENNSEDILTILENNQRIFREKQQSSKVIDPIVRE
RVGNIKRRERDGSFNDRNEAQRKKLDLPLLPTTTIGSFPQTEEIRKLRRDFKNNVISQIEYDTGIKNYIDSCIQFQEDIG
LDVLVHGEPERNDMVEYFGERLSGFVFTQNGWVQSYGSRCVKPTVIFGDVSRPKPMTIDTILYAKSKTNKIVKGMLTGPV
TILNWSYCRSDMERSAVCEQIALAIRDEINDLQKAGIKIIQVDEAAFKEGYPLRKEKVAYYENWAVKSFKLAVSSAAIET
QIHTHMCYSDFNDIIHTIEKMDADVITIETSRSGNKLLTVFATRGYKNEIGPGIYDIHSPRVPSVEELEEKIRNLMLVLP
PSKLWINPDCGLKTRKWDEIRWSLSNMVKAAQHIRLGLQRVMFV

Sequences:

>Translated_764_residues
MYTGATTYVVGFPRIGEQRELKKVLERYWDQKTSFSEIDEIAKNLRRRHWLYQKEAGISFISSNDFSLYDNMLDTAIMLN
AVPERFSNIHDKQELYFAMARGTNNTVAMEMTKWFNTNYHYIVPELSDTMNFSLCADKIINEYREAKEYGLRTKINLIGP
ITFLSLSKPVDGNQDTLELLPKILPCYVSLLKKIADLDDEVYIQFDEPIVVKDIDRRTLNLFYLCYKELSNVSNRLRLIV
MTYFDHAVEAVKILSDIPIYGIGLDFVYGQENLKVLDSLNGKKLIAGVIDGRNIWKNNYHNTLTLLKDIEKNVKKEDIIL
STTCSLLHVPYSLKHENHLDKDIKSWMSFAREKLDELSDLAKLFFVENNSEDILTILENNQRIFREKQQSSKVIDPIVRE
RVGNIKRRERDGSFNDRNEAQRKKLDLPLLPTTTIGSFPQTEEIRKLRRDFKNNVISQIEYDTGIKNYIDSCIQFQEDIG
LDVLVHGEPERNDMVEYFGERLSGFVFTQNGWVQSYGSRCVKPTVIFGDVSRPKPMTIDTILYAKSKTNKIVKGMLTGPV
TILNWSYCRSDMERSAVCEQIALAIRDEINDLQKAGIKIIQVDEAAFKEGYPLRKEKVAYYENWAVKSFKLAVSSAAIET
QIHTHMCYSDFNDIIHTIEKMDADVITIETSRSGNKLLTVFATRGYKNEIGPGIYDIHSPRVPSVEELEEKIRNLMLVLP
PSKLWINPDCGLKTRKWDEIRWSLSNMVKAAQHIRLGLQRVMFV
>Mature_764_residues
MYTGATTYVVGFPRIGEQRELKKVLERYWDQKTSFSEIDEIAKNLRRRHWLYQKEAGISFISSNDFSLYDNMLDTAIMLN
AVPERFSNIHDKQELYFAMARGTNNTVAMEMTKWFNTNYHYIVPELSDTMNFSLCADKIINEYREAKEYGLRTKINLIGP
ITFLSLSKPVDGNQDTLELLPKILPCYVSLLKKIADLDDEVYIQFDEPIVVKDIDRRTLNLFYLCYKELSNVSNRLRLIV
MTYFDHAVEAVKILSDIPIYGIGLDFVYGQENLKVLDSLNGKKLIAGVIDGRNIWKNNYHNTLTLLKDIEKNVKKEDIIL
STTCSLLHVPYSLKHENHLDKDIKSWMSFAREKLDELSDLAKLFFVENNSEDILTILENNQRIFREKQQSSKVIDPIVRE
RVGNIKRRERDGSFNDRNEAQRKKLDLPLLPTTTIGSFPQTEEIRKLRRDFKNNVISQIEYDTGIKNYIDSCIQFQEDIG
LDVLVHGEPERNDMVEYFGERLSGFVFTQNGWVQSYGSRCVKPTVIFGDVSRPKPMTIDTILYAKSKTNKIVKGMLTGPV
TILNWSYCRSDMERSAVCEQIALAIRDEINDLQKAGIKIIQVDEAAFKEGYPLRKEKVAYYENWAVKSFKLAVSSAAIET
QIHTHMCYSDFNDIIHTIEKMDADVITIETSRSGNKLLTVFATRGYKNEIGPGIYDIHSPRVPSVEELEEKIRNLMLVLP
PSKLWINPDCGLKTRKWDEIRWSLSNMVKAAQHIRLGLQRVMFV

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=761, Percent_Identity=46.2549277266754, Blast_Score=677, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=771, Percent_Identity=42.5421530479896, Blast_Score=635, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_DESRM (A4J5C3)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001113101.1
- ProteinModelPortal:   A4J5C3
- SMR:   A4J5C3
- STRING:   A4J5C3
- GeneID:   4958627
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_1751
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- PhylomeDB:   A4J5C3
- ProtClustDB:   PRK05222
- BioCyc:   DRED349161:DRED_1751-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 88374; Mature: 88374

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTGATTYVVGFPRIGEQRELKKVLERYWDQKTSFSEIDEIAKNLRRRHWLYQKEAGISF
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
ISSNDFSLYDNMLDTAIMLNAVPERFSNIHDKQELYFAMARGTNNTVAMEMTKWFNTNYH
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCCCEEEEEEHHHHCCCCE
YIVPELSDTMNFSLCADKIINEYREAKEYGLRTKINLIGPITFLSLSKPVDGNQDTLELL
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCHHHHHHH
PKILPCYVSLLKKIADLDDEVYIQFDEPIVVKDIDRRTLNLFYLCYKELSNVSNRLRLIV
HHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEE
MTYFDHAVEAVKILSDIPIYGIGLDFVYGQENLKVLDSLNGKKLIAGVIDGRNIWKNNYH
HHHHHHHHHHHHHHHCCCEEEECEEEEECCCCHHHHHCCCCCEEEEEEECCCHHHCCCCC
NTLTLLKDIEKNVKKEDIILSTTCSLLHVPYSLKHENHLDKDIKSWMSFAREKLDELSDL
HHHHHHHHHHHCCCHHCEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKLFFVENNSEDILTILENNQRIFREKQQSSKVIDPIVRERVGNIKRRERDGSFNDRNEA
HHHEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHH
QRKKLDLPLLPTTTIGSFPQTEEIRKLRRDFKNNVISQIEYDTGIKNYIDSCIQFQEDIG
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
LDVLVHGEPERNDMVEYFGERLSGFVFTQNGWVQSYGSRCVKPTVIFGDVSRPKPMTIDT
CEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCEEEEECCCCCCCCEEEH
ILYAKSKTNKIVKGMLTGPVTILNWSYCRSDMERSAVCEQIALAIRDEINDLQKAGIKII
EEEECCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
QVDEAAFKEGYPLRKEKVAYYENWAVKSFKLAVSSAAIETQIHTHMCYSDFNDIIHTIEK
EECHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDADVITIETSRSGNKLLTVFATRGYKNEIGPGIYDIHSPRVPSVEELEEKIRNLMLVLP
CCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHCEEEEEC
PSKLWINPDCGLKTRKWDEIRWSLSNMVKAAQHIRLGLQRVMFV
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYTGATTYVVGFPRIGEQRELKKVLERYWDQKTSFSEIDEIAKNLRRRHWLYQKEAGISF
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEE
ISSNDFSLYDNMLDTAIMLNAVPERFSNIHDKQELYFAMARGTNNTVAMEMTKWFNTNYH
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEECCCCCEEEEEEHHHHCCCCE
YIVPELSDTMNFSLCADKIINEYREAKEYGLRTKINLIGPITFLSLSKPVDGNQDTLELL
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCHHHHHHH
PKILPCYVSLLKKIADLDDEVYIQFDEPIVVKDIDRRTLNLFYLCYKELSNVSNRLRLIV
HHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEE
MTYFDHAVEAVKILSDIPIYGIGLDFVYGQENLKVLDSLNGKKLIAGVIDGRNIWKNNYH
HHHHHHHHHHHHHHHCCCEEEECEEEEECCCCHHHHHCCCCCEEEEEEECCCHHHCCCCC
NTLTLLKDIEKNVKKEDIILSTTCSLLHVPYSLKHENHLDKDIKSWMSFAREKLDELSDL
HHHHHHHHHHHCCCHHCEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKLFFVENNSEDILTILENNQRIFREKQQSSKVIDPIVRERVGNIKRRERDGSFNDRNEA
HHHEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHH
QRKKLDLPLLPTTTIGSFPQTEEIRKLRRDFKNNVISQIEYDTGIKNYIDSCIQFQEDIG
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
LDVLVHGEPERNDMVEYFGERLSGFVFTQNGWVQSYGSRCVKPTVIFGDVSRPKPMTIDT
CEEEEECCCCCHHHHHHHHHHHCCEEEECCCHHHHHCCCCCCCEEEEECCCCCCCCEEEH
ILYAKSKTNKIVKGMLTGPVTILNWSYCRSDMERSAVCEQIALAIRDEINDLQKAGIKII
EEEECCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
QVDEAAFKEGYPLRKEKVAYYENWAVKSFKLAVSSAAIETQIHTHMCYSDFNDIIHTIEK
EECHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDADVITIETSRSGNKLLTVFATRGYKNEIGPGIYDIHSPRVPSVEELEEKIRNLMLVLP
CCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHCEEEEEC
PSKLWINPDCGLKTRKWDEIRWSLSNMVKAAQHIRLGLQRVMFV
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA