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The map label for this gene is yitJ [H]

Identifier: 134299604

GI number: 134299604

Start: 1912354

End: 1914150

Strand: Reverse

Name: yitJ [H]

Synonym: Dred_1750

Alternate gene names: 134299604

Gene position: 1914150-1912354 (Counterclockwise)

Preceding gene: 134299605

Following gene: 134299600

Centisome position: 53.05

GC content: 33.39

Gene sequence:

>1797_bases
ATGATAAGAGAGTATTTGAAGAACAATATTCTTATTACAGATGGTGCAATGGGAACGTATTACGCCCAAATTACCGGAAA
ATATAATGTATTTCCCGAATTCGCAAACATTAACGAGCCTGAAATAATTGAAAGAATTCATACTGAATATATAAATGCCG
GGGCAAAATTGATAAAAACAAATACTTTTTCTGCTAATAGCAGGGTCCTCAAAATTCCGAAATCAGAAGTGAAAAAAATA
GTTCTAGCTGGCCTGGATCTAGCGAATAAAGTTGCCCAAGGTAAAAGCATTTTTATTGCTGCCAGTATAGGACCAATTCC
TGAGATGTTTGACAAAATGGAAACTGATCAAGAAAACAGTTTGGACGAATACAAATTTATAGTGGATATTTTCCTAAACT
CAGATATCAAAATATTTATGTTTGAAACCTTTAGTGATGTTAGTGATCTTTTAGAAATCACAAAATATATCAAAGTAAAA
GATAGTTCGGCATTTATTGTCACACAATTTGCAACAACCCCAGAAGGTTTTACCAGAAAAGGCTTAAGTAATAAGCATAT
CATAGCTGAAGTGAAAAAGAGCAAAAGTATTGATGCTTATGGATTCAATTGTGGTGTTGGACCTACTCATTTATTTAATT
CCTTGAAGAAACTAAACTTTGCAGATGATATGGTAGCTGCAGCTCCCAATGCGGGATATCCAGAGATTGTACATGAACGA
ATGGTATATATTCAAAACCCTAATTATTTTGCCGAAAAAATGAAGGATATCAGTGGACTAGGCGTTAAGATTTTAGGAGG
ATGCTGTGGCACAACTCCAATTCATATAGAAAAAATGTCTGAACTGATTCAATCATACTTAAATGCACCCATTCCTAAGC
AGCAAATTTCTGCAAAACCAAAGGTTATTATAGACAGGGAAAAAACTGATGGCTTTTTTGAAAAAATGAGAAAGGGTAAA
TTTGTTATAGCTGTAGAATTAGATCCGCCATTTAATGCTGATACAGATTTAATTTTGCAGAATGCCTGGATTTGCAAAGA
CCATGGAGTTGATGCAATCACTATAGCCGATTCTCCCATGGCAAGAGCGAGGGCAGACTCTATCATGATTGCCGCCAAAA
TCAAAAGAGAAGTTGGCATAGAGGTTTTGCCTCATCTTTGTTGTCGTGATAAAAACTTAAACGCCCTGAAATCCGCCCTT
TTAGCTGGCTACATCGAAAACATCAGAAATGTGTTAGCTATAACGGGCGATCCTGTTGCTATAGCTGATAAGAATGATAT
TAAGGGTGTATTTAACCTTAATTCCATGAAATTAATTCAACTTATTTCTACAATGAACAAAGAACTTTTTCCTGAAGATC
CAATCAATGTGGGAGGAGCATTAAACTTAAACCTATTGAAACCAGAAGCTGAGCTGGCTAAAATGGTAAAAAAGATCAAT
AGTGGAGCAACTTTTTTTCTAACTCAACCTATATTCGAGGATTATGTAATAAATATATTACGCCTAATAAACAAAAATGA
TGGTATTAAAATCTTAGGTGGTATTATGCCTCTTGTAAGCTATAAAAATGCTCTATTTTTGAATAATGAAGTGGCGGGTA
TTAAAATACCGGAAAGAATAATAAATAAATATAATAGTAATATGGGAAAAGATGAAGCTGAAATTATTGGAATTGAAATA
GCTGTAGAAATTGCTAATAAAATTAAAGAAAGCGTTGATGGTTTTTATTTTATTACACCATTTAACCGAGTTGAAATGAT
TATGAAGATTATTAAAAAATTAAAATGCTTTCACTAA

Upstream 100 bases:

>100_bases
TTTAGATGCAAGGCAAGGCAATGATGCCTTGCCTTGCATCTGTCTTTAACAGCAGACTAATTTAACTTTCTCATATTTTG
ATTTTAAAAGGTGGATTAAC

Downstream 100 bases:

>100_bases
GGTACAAAAACCTATAACCTAAAGCAAATACCCTAGAAAATAAAAACTGGCTTTTCTTCTCTCAAAAACTCCTTGGTATA
TAAAATTCACTTTTTGTAAG

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MIREYLKNNILITDGAMGTYYAQITGKYNVFPEFANINEPEIIERIHTEYINAGAKLIKTNTFSANSRVLKIPKSEVKKI
VLAGLDLANKVAQGKSIFIAASIGPIPEMFDKMETDQENSLDEYKFIVDIFLNSDIKIFMFETFSDVSDLLEITKYIKVK
DSSAFIVTQFATTPEGFTRKGLSNKHIIAEVKKSKSIDAYGFNCGVGPTHLFNSLKKLNFADDMVAAAPNAGYPEIVHER
MVYIQNPNYFAEKMKDISGLGVKILGGCCGTTPIHIEKMSELIQSYLNAPIPKQQISAKPKVIIDREKTDGFFEKMRKGK
FVIAVELDPPFNADTDLILQNAWICKDHGVDAITIADSPMARARADSIMIAAKIKREVGIEVLPHLCCRDKNLNALKSAL
LAGYIENIRNVLAITGDPVAIADKNDIKGVFNLNSMKLIQLISTMNKELFPEDPINVGGALNLNLLKPEAELAKMVKKIN
SGATFFLTQPIFEDYVINILRLINKNDGIKILGGIMPLVSYKNALFLNNEVAGIKIPERIINKYNSNMGKDEAEIIGIEI
AVEIANKIKESVDGFYFITPFNRVEMIMKIIKKLKCFH

Sequences:

>Translated_598_residues
MIREYLKNNILITDGAMGTYYAQITGKYNVFPEFANINEPEIIERIHTEYINAGAKLIKTNTFSANSRVLKIPKSEVKKI
VLAGLDLANKVAQGKSIFIAASIGPIPEMFDKMETDQENSLDEYKFIVDIFLNSDIKIFMFETFSDVSDLLEITKYIKVK
DSSAFIVTQFATTPEGFTRKGLSNKHIIAEVKKSKSIDAYGFNCGVGPTHLFNSLKKLNFADDMVAAAPNAGYPEIVHER
MVYIQNPNYFAEKMKDISGLGVKILGGCCGTTPIHIEKMSELIQSYLNAPIPKQQISAKPKVIIDREKTDGFFEKMRKGK
FVIAVELDPPFNADTDLILQNAWICKDHGVDAITIADSPMARARADSIMIAAKIKREVGIEVLPHLCCRDKNLNALKSAL
LAGYIENIRNVLAITGDPVAIADKNDIKGVFNLNSMKLIQLISTMNKELFPEDPINVGGALNLNLLKPEAELAKMVKKIN
SGATFFLTQPIFEDYVINILRLINKNDGIKILGGIMPLVSYKNALFLNNEVAGIKIPERIINKYNSNMGKDEAEIIGIEI
AVEIANKIKESVDGFYFITPFNRVEMIMKIIKKLKCFH
>Mature_598_residues
MIREYLKNNILITDGAMGTYYAQITGKYNVFPEFANINEPEIIERIHTEYINAGAKLIKTNTFSANSRVLKIPKSEVKKI
VLAGLDLANKVAQGKSIFIAASIGPIPEMFDKMETDQENSLDEYKFIVDIFLNSDIKIFMFETFSDVSDLLEITKYIKVK
DSSAFIVTQFATTPEGFTRKGLSNKHIIAEVKKSKSIDAYGFNCGVGPTHLFNSLKKLNFADDMVAAAPNAGYPEIVHER
MVYIQNPNYFAEKMKDISGLGVKILGGCCGTTPIHIEKMSELIQSYLNAPIPKQQISAKPKVIIDREKTDGFFEKMRKGK
FVIAVELDPPFNADTDLILQNAWICKDHGVDAITIADSPMARARADSIMIAAKIKREVGIEVLPHLCCRDKNLNALKSAL
LAGYIENIRNVLAITGDPVAIADKNDIKGVFNLNSMKLIQLISTMNKELFPEDPINVGGALNLNLLKPEAELAKMVKKIN
SGATFFLTQPIFEDYVINILRLINKNDGIKILGGIMPLVSYKNALFLNNEVAGIKIPERIINKYNSNMGKDEAEIIGIEI
AVEIANKIKESVDGFYFITPFNRVEMIMKIIKKLKCFH

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=321, Percent_Identity=25.5451713395639, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1790450, Length=341, Percent_Identity=22.8739002932551, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=349, Percent_Identity=24.9283667621776, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 66683; Mature: 66683

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREYLKNNILITDGAMGTYYAQITGKYNVFPEFANINEPEIIERIHTEYINAGAKLIKT
CCCHHHHCCEEEECCCCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
NTFSANSRVLKIPKSEVKKIVLAGLDLANKVAQGKSIFIAASIGPIPEMFDKMETDQENS
CCCCCCCCEEECCHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCC
LDEYKFIVDIFLNSDIKIFMFETFSDVSDLLEITKYIKVKDSSAFIVTQFATTPEGFTRK
HHHHHEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHC
GLSNKHIIAEVKKSKSIDAYGFNCGVGPTHLFNSLKKLNFADDMVAAAPNAGYPEIVHER
CCCCCHHHHEEHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHCC
MVYIQNPNYFAEKMKDISGLGVKILGGCCGTTPIHIEKMSELIQSYLNAPIPKQQISAKP
EEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCHHHHCCCC
KVIIDREKTDGFFEKMRKGKFVIAVELDPPFNADTDLILQNAWICKDHGVDAITIADSPM
CEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCEEEECCCCCEEEECCCCH
ARARADSIMIAAKIKREVGIEVLPHLCCRDKNLNALKSALLAGYIENIRNVLAITGDPVA
HHHHHCCEEEEEHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCEE
IADKNDIKGVFNLNSMKLIQLISTMNKELFPEDPINVGGALNLNLLKPEAELAKMVKKIN
EECCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHC
SGATFFLTQPIFEDYVINILRLINKNDGIKILGGIMPLVSYKNALFLNNEVAGIKIPERI
CCCEEEEECHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCEEEECCCCCCCCCHHHH
INKYNSNMGKDEAEIIGIEIAVEIANKIKESVDGFYFITPFNRVEMIMKIIKKLKCFH
HHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIREYLKNNILITDGAMGTYYAQITGKYNVFPEFANINEPEIIERIHTEYINAGAKLIKT
CCCHHHHCCEEEECCCCCEEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
NTFSANSRVLKIPKSEVKKIVLAGLDLANKVAQGKSIFIAASIGPIPEMFDKMETDQENS
CCCCCCCCEEECCHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCC
LDEYKFIVDIFLNSDIKIFMFETFSDVSDLLEITKYIKVKDSSAFIVTQFATTPEGFTRK
HHHHHEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHEEEECCCEEEEEEECCCCCCHHHC
GLSNKHIIAEVKKSKSIDAYGFNCGVGPTHLFNSLKKLNFADDMVAAAPNAGYPEIVHER
CCCCCHHHHEEHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHCC
MVYIQNPNYFAEKMKDISGLGVKILGGCCGTTPIHIEKMSELIQSYLNAPIPKQQISAKP
EEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCHHHHCCCC
KVIIDREKTDGFFEKMRKGKFVIAVELDPPFNADTDLILQNAWICKDHGVDAITIADSPM
CEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCEEEECCCCCEEEECCCCH
ARARADSIMIAAKIKREVGIEVLPHLCCRDKNLNALKSALLAGYIENIRNVLAITGDPVA
HHHHHCCEEEEEHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCEE
IADKNDIKGVFNLNSMKLIQLISTMNKELFPEDPINVGGALNLNLLKPEAELAKMVKKIN
EECCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHC
SGATFFLTQPIFEDYVINILRLINKNDGIKILGGIMPLVSYKNALFLNNEVAGIKIPERI
CCCEEEEECHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCEEEECCCCCCCCCHHHH
INKYNSNMGKDEAEIIGIEIAVEIANKIKESVDGFYFITPFNRVEMIMKIIKKLKCFH
HHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]