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The map label for this gene is nuoF2 [H]

Identifier: 134299509

GI number: 134299509

Start: 1807554

End: 1809263

Strand: Reverse

Name: nuoF2 [H]

Synonym: Dred_1652

Alternate gene names: 134299509

Gene position: 1809263-1807554 (Counterclockwise)

Preceding gene: 134299510

Following gene: 134299508

Centisome position: 50.14

GC content: 45.2

Gene sequence:

>1710_bases
ATGAATTTTAAAGAATTAGAATTGCTAAGAGCAACTGCTAAGGAAGGTATTAAATTACGTTTGGGTGAAGAACCATCCGA
CAATTTCAAATATCACGTTTTAGTATGCGGCGGTACCGGATGTCATTCAGTTGGTTGTCAAAATACTTTGCAAGCTCTGC
AAAAGGCAATTGACAGCCAAGGCTTGCAAGATACAGTTAAATTAGTGGTTACAGGTTGTATGGGTACCTGTGAAATGGGA
CCTGTAATAACAGTATTCCCAGATGGTTATTATTACTGTCGTGTTCAACCCGAGGATGCCGAGGAACTGGTTACTTCTCA
TCTGAAGGAAGGCAAGTATGTGGATCGTCTGTGCTATCACACTGCTGATGGAACTGTACAACCCCGCAAATCTGACATGA
CTTTCTTTAAAAAGCAAAAACGGAATGCACTCCGTAACTGTGGTCTAATCAACCAAACTTCTATCGAAGAATACATTGCA
GTGGGTGGATTTAAAGCACTGGCTAAGACTCTGCAGGAAATGACTCCCCAGCAGGTCGTAGACGAAGTGAAAAAATCCGG
TCTGCGTGGTCGGGGTGGCGGCGGTTTTCCCACTGGTTTAAAATGGCAATTTGTACTAGACCAAAAATCCGACCAGAAAT
ATGTTGTTTGCAACGGTGACGAGGGTGACCCGGGTGCCTTCATGGATCGTTCTGTATTAGAAGATGATCCCTATTCTGTC
ATTGAAGCTATGACCATTGGCGGGTATGCAGTGGGCGCCAATAAGGGTTATGCTTACATTCGTTTAGAGTATCCCAAAGC
CATTGATCACTTTAATAAGGCCCTTGCCCGTGCCAGAGAAGTGGGCTTGCTGGGTAACAACATTTTAGAATCAGATTTCA
GTTTTGATATCGAAATTCGTGTGGGTGCCGGAGCTTTTGTGTGCGGCGAAGAAACTGCCCTCTTAGCCTCTATTGAAGGT
CGCCGTGGTGAGCCTCGTCCACGCCCCCCCTTCCCTGCTGTCTCCGGTTTATTTGAGAAACCAACCCTGCTGAATAACGT
TGAAACCTATGCTAGTATCTGCCCCATTATTATTAACGGTGGTGAATGGTATGCCACCATGGGTACAGAAAAGAGTAAAG
GCACCAAGGTTTTCACTTTGGCCGGTAAGATTAACAACACTGGTTTAGTAGAAATTCCATTAGGCCTTACTTTACGCGAA
ATGGTAGAAGACATTGGCGGCGGAGTACCAAATGGCAAAGCCTTTAAAGCTGCCCAAACCGGAGGTCCATCCGGCGGTTG
TATTCCTGCTGCTTATTTAGATACTCCTATTGATTATGAAAACCTAGCAGCCCTTGGTTCTATCGTTGGTTCAGGCGGTA
TGATCATTGTAGACGAAAGTACCTGTATGGTAGACCTAGCTAAATTCTATTTAGGCTTTACGCAGGATGAATCCTGCGGC
AAATGTACCCCTTGCCGTATTGGTACCAAGAGAATGCTGGAAATACTCGAGCGTATTACAAAGGGATTAGGCAAGCCAGA
GGATTTAGATCTATTAGTTGAACTGGCCAACTCTATCAAGGCCTCTGCCCTTTGTGGATTAGGCCAAACTGCTCCCAATC
CCATTCTTAGCACCTTGCGTTATTTCCGTGATGAATTTGAAGCCCACATTAAGGATAAAACCTGCCCGGCTGGCGTTTGC
AAAATGCCCCGCAAAGGCCTAAAATCCTAG

Upstream 100 bases:

>100_bases
TATTGACAGTAAACGGTGAAGTTTATCCCCGTATGACTGCAGAAAAAGTAACTGAAGTGTTAAGCAAATATGAATAATAC
CTCCAGGGAGGTGAAGATAA

Downstream 100 bases:

>100_bases
AGGATAGGAGGGAAAAGGCATGGCAACTGTAACACTAACCATTGACAATGTAAAAGTTGAAGTTGAACAAGGTTCAACGA
TATTAGATGCTGCGAAAAAA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MNFKELELLRATAKEGIKLRLGEEPSDNFKYHVLVCGGTGCHSVGCQNTLQALQKAIDSQGLQDTVKLVVTGCMGTCEMG
PVITVFPDGYYYCRVQPEDAEELVTSHLKEGKYVDRLCYHTADGTVQPRKSDMTFFKKQKRNALRNCGLINQTSIEEYIA
VGGFKALAKTLQEMTPQQVVDEVKKSGLRGRGGGGFPTGLKWQFVLDQKSDQKYVVCNGDEGDPGAFMDRSVLEDDPYSV
IEAMTIGGYAVGANKGYAYIRLEYPKAIDHFNKALARAREVGLLGNNILESDFSFDIEIRVGAGAFVCGEETALLASIEG
RRGEPRPRPPFPAVSGLFEKPTLLNNVETYASICPIIINGGEWYATMGTEKSKGTKVFTLAGKINNTGLVEIPLGLTLRE
MVEDIGGGVPNGKAFKAAQTGGPSGGCIPAAYLDTPIDYENLAALGSIVGSGGMIIVDESTCMVDLAKFYLGFTQDESCG
KCTPCRIGTKRMLEILERITKGLGKPEDLDLLVELANSIKASALCGLGQTAPNPILSTLRYFRDEFEAHIKDKTCPAGVC
KMPRKGLKS

Sequences:

>Translated_569_residues
MNFKELELLRATAKEGIKLRLGEEPSDNFKYHVLVCGGTGCHSVGCQNTLQALQKAIDSQGLQDTVKLVVTGCMGTCEMG
PVITVFPDGYYYCRVQPEDAEELVTSHLKEGKYVDRLCYHTADGTVQPRKSDMTFFKKQKRNALRNCGLINQTSIEEYIA
VGGFKALAKTLQEMTPQQVVDEVKKSGLRGRGGGGFPTGLKWQFVLDQKSDQKYVVCNGDEGDPGAFMDRSVLEDDPYSV
IEAMTIGGYAVGANKGYAYIRLEYPKAIDHFNKALARAREVGLLGNNILESDFSFDIEIRVGAGAFVCGEETALLASIEG
RRGEPRPRPPFPAVSGLFEKPTLLNNVETYASICPIIINGGEWYATMGTEKSKGTKVFTLAGKINNTGLVEIPLGLTLRE
MVEDIGGGVPNGKAFKAAQTGGPSGGCIPAAYLDTPIDYENLAALGSIVGSGGMIIVDESTCMVDLAKFYLGFTQDESCG
KCTPCRIGTKRMLEILERITKGLGKPEDLDLLVELANSIKASALCGLGQTAPNPILSTLRYFRDEFEAHIKDKTCPAGVC
KMPRKGLKS
>Mature_569_residues
MNFKELELLRATAKEGIKLRLGEEPSDNFKYHVLVCGGTGCHSVGCQNTLQALQKAIDSQGLQDTVKLVVTGCMGTCEMG
PVITVFPDGYYYCRVQPEDAEELVTSHLKEGKYVDRLCYHTADGTVQPRKSDMTFFKKQKRNALRNCGLINQTSIEEYIA
VGGFKALAKTLQEMTPQQVVDEVKKSGLRGRGGGGFPTGLKWQFVLDQKSDQKYVVCNGDEGDPGAFMDRSVLEDDPYSV
IEAMTIGGYAVGANKGYAYIRLEYPKAIDHFNKALARAREVGLLGNNILESDFSFDIEIRVGAGAFVCGEETALLASIEG
RRGEPRPRPPFPAVSGLFEKPTLLNNVETYASICPIIINGGEWYATMGTEKSKGTKVFTLAGKINNTGLVEIPLGLTLRE
MVEDIGGGVPNGKAFKAAQTGGPSGGCIPAAYLDTPIDYENLAALGSIVGSGGMIIVDESTCMVDLAKFYLGFTQDESCG
KCTPCRIGTKRMLEILERITKGLGKPEDLDLLVELANSIKASALCGLGQTAPNPILSTLRYFRDEFEAHIKDKTCPAGVC
KMPRKGLKS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=453, Percent_Identity=36.8653421633554, Blast_Score=298, Evalue=7e-81,
Organism=Homo sapiens, GI260656005, Length=380, Percent_Identity=40.7894736842105, Blast_Score=298, Evalue=1e-80,
Organism=Escherichia coli, GI1788620, Length=399, Percent_Identity=41.3533834586466, Blast_Score=337, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI17531727, Length=382, Percent_Identity=39.7905759162304, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI19920794, Length=377, Percent_Identity=39.7877984084881, Blast_Score=293, Evalue=3e-79,
Organism=Drosophila melanogaster, GI45550452, Length=380, Percent_Identity=38.9473684210526, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24653819, Length=385, Percent_Identity=33.2467532467532, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=30.9859154929577, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 61623; Mature: 61623

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKELELLRATAKEGIKLRLGEEPSDNFKYHVLVCGGTGCHSVGCQNTLQALQKAIDSQ
CCHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
GLQDTVKLVVTGCMGTCEMGPVITVFPDGYYYCRVQPEDAEELVTSHLKEGKYVDRLCYH
CHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHC
TADGTVQPRKSDMTFFKKQKRNALRNCGLINQTSIEEYIAVGGFKALAKTLQEMTPQQVV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHH
DEVKKSGLRGRGGGGFPTGLKWQFVLDQKSDQKYVVCNGDEGDPGAFMDRSVLEDDPYSV
HHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHCCHHCCCCCHHH
IEAMTIGGYAVGANKGYAYIRLEYPKAIDHFNKALARAREVGLLGNNILESDFSFDIEIR
HHHHHCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEE
VGAGAFVCGEETALLASIEGRRGEPRPRPPFPAVSGLFEKPTLLNNVETYASICPIIING
ECCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEC
GEWYATMGTEKSKGTKVFTLAGKINNTGLVEIPLGLTLREMVEDIGGGVPNGKAFKAAQT
CEEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEECC
GGPSGGCIPAAYLDTPIDYENLAALGSIVGSGGMIIVDESTCMVDLAKFYLGFTQDESCG
CCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC
KCTPCRIGTKRMLEILERITKGLGKPEDLDLLVELANSIKASALCGLGQTAPNPILSTLR
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
YFRDEFEAHIKDKTCPAGVCKMPRKGLKS
HHHHHHHHHHCCCCCCCHHHHCCHHHCCC
>Mature Secondary Structure
MNFKELELLRATAKEGIKLRLGEEPSDNFKYHVLVCGGTGCHSVGCQNTLQALQKAIDSQ
CCHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCC
GLQDTVKLVVTGCMGTCEMGPVITVFPDGYYYCRVQPEDAEELVTSHLKEGKYVDRLCYH
CHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHHC
TADGTVQPRKSDMTFFKKQKRNALRNCGLINQTSIEEYIAVGGFKALAKTLQEMTPQQVV
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCHHHHH
DEVKKSGLRGRGGGGFPTGLKWQFVLDQKSDQKYVVCNGDEGDPGAFMDRSVLEDDPYSV
HHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHCCHHCCCCCHHH
IEAMTIGGYAVGANKGYAYIRLEYPKAIDHFNKALARAREVGLLGNNILESDFSFDIEIR
HHHHHCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEEEE
VGAGAFVCGEETALLASIEGRRGEPRPRPPFPAVSGLFEKPTLLNNVETYASICPIIING
ECCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCEEEEC
GEWYATMGTEKSKGTKVFTLAGKINNTGLVEIPLGLTLREMVEDIGGGVPNGKAFKAAQT
CEEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEECC
GGPSGGCIPAAYLDTPIDYENLAALGSIVGSGGMIIVDESTCMVDLAKFYLGFTQDESCG
CCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC
KCTPCRIGTKRMLEILERITKGLGKPEDLDLLVELANSIKASALCGLGQTAPNPILSTLR
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
YFRDEFEAHIKDKTCPAGVCKMPRKGLKS
HHHHHHHHHHCCCCCCCHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]