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The map label for this gene is slt [H]

Identifier: 134299452

GI number: 134299452

Start: 1735080

End: 1735652

Strand: Reverse

Name: slt [H]

Synonym: Dred_1595

Alternate gene names: 134299452

Gene position: 1735652-1735080 (Counterclockwise)

Preceding gene: 134299453

Following gene: 134299451

Centisome position: 48.1

GC content: 43.98

Gene sequence:

>573_bases
GTGACTATTCGAAGCAAAAAAAATAGAGGCTTTAAAAGAAGGCTCCTGCTTCTGGTTTTGCTAGTACTAATCTGGTTCAG
CCATAAAGAAATCGGGAAGTTATGGTACCCCTTTAAGTACCAGGAGTTAATTTATCATTATGCCGAAGTTAACCACCTAG
ACCCTTTGCTGGTGGCAGCGGTTATTAAAACCGAAAGCAATTTTAATCCCAGAGCCACGTCCCCCAAGGGGGCCCGGGGC
CTCATGCAAATTATGCCAGACACCGCCAATTGGATATCCCAACAAATTGGCAGCGGTCCCCTGAAGTCAGAAAACCTACT
CTATCCGGAAACCAGTATCAGTTTAGGCACTTGGTACTTATCGGATCTATTTTCCGAATTTAAAAATGATCCAGTTTTGG
TGATTGCTGCCTATAATGCCGGGCGCGGCAATGTTACTCATTGGTTAAACGAGCAACACTGGACCGGAGAAAGAAAAACC
ATTGATCAGATTCCCTTTATGGAAACCAGACAGTATATCCGGCGAGTCCTTTGGAACCAGAAAGTTTATCATTACCTTTA
CGGTATGGAGTAA

Upstream 100 bases:

>100_bases
AATTAATAGTTATTTGGTCCAACTTAATAAATTATTTACATGATTAACCATTTTCTGGTATATAATGTATTGGTTAAGTT
TGTTTGCAGGAGTGATGACA

Downstream 100 bases:

>100_bases
AAAAAAGTCATTCCTTGCGAATGACTTTTTTATTTTCTGGATCTTATGGTATTTCTTAACTTACGATATTTTGTTTAATT
AATTGTATAATAGAGTCGAT

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTIRSKKNRGFKRRLLLLVLLVLIWFSHKEIGKLWYPFKYQELIYHYAEVNHLDPLLVAAVIKTESNFNPRATSPKGARG
LMQIMPDTANWISQQIGSGPLKSENLLYPETSISLGTWYLSDLFSEFKNDPVLVIAAYNAGRGNVTHWLNEQHWTGERKT
IDQIPFMETRQYIRRVLWNQKVYHYLYGME

Sequences:

>Translated_190_residues
MTIRSKKNRGFKRRLLLLVLLVLIWFSHKEIGKLWYPFKYQELIYHYAEVNHLDPLLVAAVIKTESNFNPRATSPKGARG
LMQIMPDTANWISQQIGSGPLKSENLLYPETSISLGTWYLSDLFSEFKNDPVLVIAAYNAGRGNVTHWLNEQHWTGERKT
IDQIPFMETRQYIRRVLWNQKVYHYLYGME
>Mature_189_residues
TIRSKKNRGFKRRLLLLVLLVLIWFSHKEIGKLWYPFKYQELIYHYAEVNHLDPLLVAAVIKTESNFNPRATSPKGARGL
MQIMPDTANWISQQIGSGPLKSENLLYPETSISLGTWYLSDLFSEFKNDPVLVIAAYNAGRGNVTHWLNEQHWTGERKTI
DQIPFMETRQYIRRVLWNQKVYHYLYGME

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=165, Percent_Identity=37.5757575757576, Blast_Score=101, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22333; Mature: 22202

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRSKKNRGFKRRLLLLVLLVLIWFSHKEIGKLWYPFKYQELIYHYAEVNHLDPLLVAA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
VIKTESNFNPRATSPKGARGLMQIMPDTANWISQQIGSGPLKSENLLYPETSISLGTWYL
HHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH
SDLFSEFKNDPVLVIAAYNAGRGNVTHWLNEQHWTGERKTIDQIPFMETRQYIRRVLWNQ
HHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCH
KVYHYLYGME
HHHHHHHCCC
>Mature Secondary Structure 
TIRSKKNRGFKRRLLLLVLLVLIWFSHKEIGKLWYPFKYQELIYHYAEVNHLDPLLVAA
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHH
VIKTESNFNPRATSPKGARGLMQIMPDTANWISQQIGSGPLKSENLLYPETSISLGTWYL
HHHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH
SDLFSEFKNDPVLVIAAYNAGRGNVTHWLNEQHWTGERKTIDQIPFMETRQYIRRVLWNQ
HHHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCH
KVYHYLYGME
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]