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The map label for this gene is bioB

Identifier: 134298305

GI number: 134298305

Start: 470524

End: 471489

Strand: Reverse

Name: bioB

Synonym: Dred_0430

Alternate gene names: 134298305

Gene position: 471489-470524 (Counterclockwise)

Preceding gene: 134298306

Following gene: 134298304

Centisome position: 13.07

GC content: 40.58

Gene sequence:

>966_bases
ATGTTTTCTGAAATCCAGGAGAAAATTCACAGCGGAGAGGGTATTACTTTCCAGGAAGCAGCATACCTCTCCAAAGCTGA
AAACCTCGATATACTTCAAATACTTTCAATGGCAGCCCAGGTTACAGCCAATTTTGCCAATAAGAAAATAGATTTATGCT
CAATTGTAAATGCTAAATCTGGGAGTTGCTCGGAGGATTGTAAATTCTGTGCCCAATCGGTTTATTACGCAACGGGCTGC
CAGACTTACCCTTTGCTTAATTCTGTTGCGATATTGGAAGCCGCCAAAAGGGTTGAGGAAAAGGGTATTCACCGCTTTGC
CCTCGTCACCAGTGGAAAAAATCTTAGCAATCGTGATTTTGATGAGGTCATTAAAATTTATCAAGTACTCCGAGAAAAGA
CCTCCTTGCAGTTGTGCGCTTCCTTAGGATTACTTGATACATCAAGGGCTTTACAACTCAAGAAGGCGGGTGTTTCTACC
TACCACCATAACTTGGAGTGTGCAGAAAGTTTTTTTAAAAGAATTTGTACCACTCATACCTATCAGCAGCGTGTGGCTAC
AATAAAATCTGCCCAAAGTGCAGGATTAAAAATTTGTAGTGGCGGCATTATCTCCCTTGGCGAAACCATGTTACAACGCT
TGGAACTGGCCTATGAGTTGAAAGCTTTGGGAGTAGATTCAGTACCAATTAATGTTCTTAATCCTATCTTGGGAACCCCT
CTAGCTGGACAACAAATTATGTCTTCACAGGATATTATTAAGACAATTTGTCTCTTTAGATTGATTCTACCCACGATTTC
TCTTCGATTTGGTGGAGGTATAAAAGAATCCCTAGGAGAATTAAGAGTACTAGGTTTTCCAGCGGGAATCAATGCTGTCA
TTATTGGAAACTTCTTAACCACTACTGGCTACCAAATTGATCGGGAATTAAGTGTTATCAGGAGTATGGGGCTCAATATT
TCTTAA

Upstream 100 bases:

>100_bases
TTTAAACCTTTGTAAGAAAATTACCTATTGTAAAATCTTGGGTAGTTTGTTATTGTTAACTTGTAATCAATAGTTTGGTT
GACAATAAAGGAGGGCCTCC

Downstream 100 bases:

>100_bases
GACTTGTGCCGAAACTTTCGTGCCCTTTCATGCATGGTGCATACTAAGGGCCTAATACAGTCAAGTTTGCTAAGAAAGTG
AAGTATGCTCATGTCGAAAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MFSEIQEKIHSGEGITFQEAAYLSKAENLDILQILSMAAQVTANFANKKIDLCSIVNAKSGSCSEDCKFCAQSVYYATGC
QTYPLLNSVAILEAAKRVEEKGIHRFALVTSGKNLSNRDFDEVIKIYQVLREKTSLQLCASLGLLDTSRALQLKKAGVST
YHHNLECAESFFKRICTTHTYQQRVATIKSAQSAGLKICSGGIISLGETMLQRLELAYELKALGVDSVPINVLNPILGTP
LAGQQIMSSQDIIKTICLFRLILPTISLRFGGGIKESLGELRVLGFPAGINAVIIGNFLTTTGYQIDRELSVIRSMGLNI
S

Sequences:

>Translated_321_residues
MFSEIQEKIHSGEGITFQEAAYLSKAENLDILQILSMAAQVTANFANKKIDLCSIVNAKSGSCSEDCKFCAQSVYYATGC
QTYPLLNSVAILEAAKRVEEKGIHRFALVTSGKNLSNRDFDEVIKIYQVLREKTSLQLCASLGLLDTSRALQLKKAGVST
YHHNLECAESFFKRICTTHTYQQRVATIKSAQSAGLKICSGGIISLGETMLQRLELAYELKALGVDSVPINVLNPILGTP
LAGQQIMSSQDIIKTICLFRLILPTISLRFGGGIKESLGELRVLGFPAGINAVIIGNFLTTTGYQIDRELSVIRSMGLNI
S
>Mature_321_residues
MFSEIQEKIHSGEGITFQEAAYLSKAENLDILQILSMAAQVTANFANKKIDLCSIVNAKSGSCSEDCKFCAQSVYYATGC
QTYPLLNSVAILEAAKRVEEKGIHRFALVTSGKNLSNRDFDEVIKIYQVLREKTSLQLCASLGLLDTSRALQLKKAGVST
YHHNLECAESFFKRICTTHTYQQRVATIKSAQSAGLKICSGGIISLGETMLQRLELAYELKALGVDSVPINVLNPILGTP
LAGQQIMSSQDIIKTICLFRLILPTISLRFGGGIKESLGELRVLGFPAGINAVIIGNFLTTTGYQIDRELSVIRSMGLNI
S

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=293, Percent_Identity=35.4948805460751, Blast_Score=194, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321725, Length=282, Percent_Identity=31.9148936170213, Blast_Score=159, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_DESRM (A4J1M3)

Other databases:

- EMBL:   CP000612
- RefSeq:   YP_001111801.1
- ProteinModelPortal:   A4J1M3
- SMR:   A4J1M3
- STRING:   A4J1M3
- GeneID:   4955407
- GenomeReviews:   CP000612_GR
- KEGG:   drm:Dred_0430
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   A4J1M3
- BioCyc:   DRED349161:DRED_0430-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 34996; Mature: 34996

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSEIQEKIHSGEGITFQEAAYLSKAENLDILQILSMAAQVTANFANKKIDLCSIVNAKS
CCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
GSCSEDCKFCAQSVYYATGCQTYPLLNSVAILEAAKRVEEKGIHRFALVTSGKNLSNRDF
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCH
DEVIKIYQVLREKTSLQLCASLGLLDTSRALQLKKAGVSTYHHNLECAESFFKRICTTHT
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHH
YQQRVATIKSAQSAGLKICSGGIISLGETMLQRLELAYELKALGVDSVPINVLNPILGTP
HHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
LAGQQIMSSQDIIKTICLFRLILPTISLRFGGGIKESLGELRVLGFPAGINAVIIGNFLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCEEEECCHHH
TTGYQIDRELSVIRSMGLNIS
HCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFSEIQEKIHSGEGITFQEAAYLSKAENLDILQILSMAAQVTANFANKKIDLCSIVNAKS
CCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCC
GSCSEDCKFCAQSVYYATGCQTYPLLNSVAILEAAKRVEEKGIHRFALVTSGKNLSNRDF
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCH
DEVIKIYQVLREKTSLQLCASLGLLDTSRALQLKKAGVSTYHHNLECAESFFKRICTTHT
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHH
YQQRVATIKSAQSAGLKICSGGIISLGETMLQRLELAYELKALGVDSVPINVLNPILGTP
HHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
LAGQQIMSSQDIIKTICLFRLILPTISLRFGGGIKESLGELRVLGFPAGINAVIIGNFLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCCCEEEECCHHH
TTGYQIDRELSVIRSMGLNIS
HCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA