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The map label for this gene is hcp [H]

Identifier: 134298298

GI number: 134298298

Start: 462378

End: 463685

Strand: Reverse

Name: hcp [H]

Synonym: Dred_0423

Alternate gene names: 134298298

Gene position: 463685-462378 (Counterclockwise)

Preceding gene: 134298302

Following gene: 134298297

Centisome position: 12.85

GC content: 47.4

Gene sequence:

>1308_bases
ATGTTTTGCTACCAGTGTGAACAAACTCCCAAAGGCGGCTGTACAAAAATCGGGGTCTGTGGCAAGGACGAAAATATTGC
CAGCTTACAAGATACCATTATTTTTGCACTTAAGGGCGTAGCTGCATACGCTACCCATGCTAAGGAACTGGGTTACACCG
ATGCTGAAGTGGATGCAATTACCCAAGAAGCTCTCTACTCTACCCTAACCAACGTTAACTTTAATCTGGAAGAAACCATC
AATATGGCTCTGAAAGTGGGTACTGCCACTGTTAAGGTCATGGACCTGCTGGACAAGGCTCACCTGGATAATCTGGGTGT
TCCCACCCCTGTTACCGTCACTTCCGATAAAGTTGAAGGTAAGTGCATCCTGGTGACCGGTCACGATTTACTAGCTCTAA
AAGAATTGTTACAACAATCCGAAGGTAAAGGTATTAACATCTACACCCACTCTGAAATGCTGCCTGCCCACGGCTACCCT
GAATTAAAGAAGTTCAGTCATTTAAAAGGCAATGTGGGTAAAGCCTGGTACGATCAAAGGAAGCTCTTCGAAGCTTTCCC
CGGTGCAATCCTGGCCACCACCAACTGTGTAATGCCCATTCGGAAAGATGCCACCTATGGTGACCGCATGTTTTCCTTCG
GTGTTACCGGTTTAGAAGGCGTTAAGAAAATTGAAGGCATGGATTTTACCCCGGTAATTGAAAAGGCTCTTTCCTTACCC
GGTGCTCCTGCCCAAGATCTGACCGCAGAAGCAAAAACCATGACCACTGGTTTCCACCATCTAAATGTACTGCCCCTGGC
TCCCCAAATTGTGGAAGCTGTGAAGGCTGGTAAGATCAAGCGTTTCTTTGTAATTGCTGGTTGCGACTCTCCCACCAAGG
GCAGAGATTACTTCAGAGAACTGGCTTTGGCTATTCCAAAGGATTGTGTCCTTCTGACCACTTCCTGCGGTAAGTTCAGA
TTCAATGATATCGACTTTGGCACCATTGAAGGCACTGGTATCCCCCGTTTCCTCGACCTGGGTCAGTGCAACAACTCCGG
TTCTGCGGTAAAGATCGCTCTGGCTCTAGCCGAAGCTTTCAATTGCGGCGTCAATGATCTACCCTTAAGCATTGTATTGT
CCTGGTTCGAGCAAAAGGCAGTAGCCATTCTGTTAGGCCTGTTTAGCCTTGGTGTTAAGAACATGTATATCGGACCTAAG
GCACCTGAATTCTTGACACCTGGAGTTGTAGAAGTTCTGCAGAAGACCTTCAACCTGCAACTGATCTCCGGAGATGCCAA
CGCCGACCTGGTTAAAATGCTGGGTTAA

Upstream 100 bases:

>100_bases
AGTGTGATACTGGTCACATCTTCCTCCTTTGGTTTCCGATATGATTACATCAGAAAACAAAACACGGCCAAGGCCGATAA
ATATAAGGAGGAATTTAACT

Downstream 100 bases:

>100_bases
TAGATAAACTGCCAGTATCATAAGGTTCACCTCCTTATTAAAAGAGGCTTCAGCCAACCGGCTAAAGCCTCTTTTAAGTT
TATTCATGTATTCCCTACTG

Product: hydroxylamine reductase

Products: hydroxylamine; reduced acceptor

Alternate protein names: Hybrid-cluster protein; HCP [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MFCYQCEQTPKGGCTKIGVCGKDENIASLQDTIIFALKGVAAYATHAKELGYTDAEVDAITQEALYSTLTNVNFNLEETI
NMALKVGTATVKVMDLLDKAHLDNLGVPTPVTVTSDKVEGKCILVTGHDLLALKELLQQSEGKGINIYTHSEMLPAHGYP
ELKKFSHLKGNVGKAWYDQRKLFEAFPGAILATTNCVMPIRKDATYGDRMFSFGVTGLEGVKKIEGMDFTPVIEKALSLP
GAPAQDLTAEAKTMTTGFHHLNVLPLAPQIVEAVKAGKIKRFFVIAGCDSPTKGRDYFRELALAIPKDCVLLTTSCGKFR
FNDIDFGTIEGTGIPRFLDLGQCNNSGSAVKIALALAEAFNCGVNDLPLSIVLSWFEQKAVAILLGLFSLGVKNMYIGPK
APEFLTPGVVEVLQKTFNLQLISGDANADLVKMLG

Sequences:

>Translated_435_residues
MFCYQCEQTPKGGCTKIGVCGKDENIASLQDTIIFALKGVAAYATHAKELGYTDAEVDAITQEALYSTLTNVNFNLEETI
NMALKVGTATVKVMDLLDKAHLDNLGVPTPVTVTSDKVEGKCILVTGHDLLALKELLQQSEGKGINIYTHSEMLPAHGYP
ELKKFSHLKGNVGKAWYDQRKLFEAFPGAILATTNCVMPIRKDATYGDRMFSFGVTGLEGVKKIEGMDFTPVIEKALSLP
GAPAQDLTAEAKTMTTGFHHLNVLPLAPQIVEAVKAGKIKRFFVIAGCDSPTKGRDYFRELALAIPKDCVLLTTSCGKFR
FNDIDFGTIEGTGIPRFLDLGQCNNSGSAVKIALALAEAFNCGVNDLPLSIVLSWFEQKAVAILLGLFSLGVKNMYIGPK
APEFLTPGVVEVLQKTFNLQLISGDANADLVKMLG
>Mature_435_residues
MFCYQCEQTPKGGCTKIGVCGKDENIASLQDTIIFALKGVAAYATHAKELGYTDAEVDAITQEALYSTLTNVNFNLEETI
NMALKVGTATVKVMDLLDKAHLDNLGVPTPVTVTSDKVEGKCILVTGHDLLALKELLQQSEGKGINIYTHSEMLPAHGYP
ELKKFSHLKGNVGKAWYDQRKLFEAFPGAILATTNCVMPIRKDATYGDRMFSFGVTGLEGVKKIEGMDFTPVIEKALSLP
GAPAQDLTAEAKTMTTGFHHLNVLPLAPQIVEAVKAGKIKRFFVIAGCDSPTKGRDYFRELALAIPKDCVLLTTSCGKFR
FNDIDFGTIEGTGIPRFLDLGQCNNSGSAVKIALALAEAFNCGVNDLPLSIVLSWFEQKAVAILLGLFSLGVKNMYIGPK
APEFLTPGVVEVLQKTFNLQLISGDANADLVKMLG

Specific function: Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O [H]

COG id: COG1151

COG function: function code C; 6Fe-6S prismane cluster-containing protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HCP family [H]

Homologues:

Organism=Escherichia coli, GI87081794, Length=417, Percent_Identity=43.4052757793765, Blast_Score=354, Evalue=5e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010048
- InterPro:   IPR004137
- InterPro:   IPR011254
- InterPro:   IPR016099
- InterPro:   IPR016100 [H]

Pfam domain/function: PF03063 Prismane [H]

EC number: 1.7.99.1

Molecular weight: Translated: 46979; Mature: 46979

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFCYQCEQTPKGGCTKIGVCGKDENIASLQDTIIFALKGVAAYATHAKELGYTDAEVDAI
CCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
TQEALYSTLTNVNFNLEETINMALKVGTATVKVMDLLDKAHLDNLGVPTPVTVTSDKVEG
HHHHHHHHHHCCCCCHHHHHHHHHEECHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
KCILVTGHDLLALKELLQQSEGKGINIYTHSEMLPAHGYPELKKFSHLKGNVGKAWYDQR
EEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
KLFEAFPGAILATTNCVMPIRKDATYGDRMFSFGVTGLEGVKKIEGMDFTPVIEKALSLP
HHHHHCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCHHHHHHHCCCCCHHHHHHHHCCC
GAPAQDLTAEAKTMTTGFHHLNVLPLAPQIVEAVKAGKIKRFFVIAGCDSPTKGRDYFRE
CCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHH
LALAIPKDCVLLTTSCGKFRFNDIDFGTIEGTGIPRFLDLGQCNNSGSAVKIALALAEAF
HHHHCCCCEEEEEECCCCEEECCCCCEEECCCCCCCEEECCCCCCCCCEEEHHHHHHHHH
NCGVNDLPLSIVLSWFEQKAVAILLGLFSLGVKNMYIGPKAPEFLTPGVVEVLQKTFNLQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCEE
LISGDANADLVKMLG
EEECCCCCHHHHHCC
>Mature Secondary Structure
MFCYQCEQTPKGGCTKIGVCGKDENIASLQDTIIFALKGVAAYATHAKELGYTDAEVDAI
CCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
TQEALYSTLTNVNFNLEETINMALKVGTATVKVMDLLDKAHLDNLGVPTPVTVTSDKVEG
HHHHHHHHHHCCCCCHHHHHHHHHEECHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
KCILVTGHDLLALKELLQQSEGKGINIYTHSEMLPAHGYPELKKFSHLKGNVGKAWYDQR
EEEEEECCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
KLFEAFPGAILATTNCVMPIRKDATYGDRMFSFGVTGLEGVKKIEGMDFTPVIEKALSLP
HHHHHCCCCEEECCCCEEECCCCCCCCCHHHHCCCCCHHHHHHHCCCCCHHHHHHHHCCC
GAPAQDLTAEAKTMTTGFHHLNVLPLAPQIVEAVKAGKIKRFFVIAGCDSPTKGRDYFRE
CCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHH
LALAIPKDCVLLTTSCGKFRFNDIDFGTIEGTGIPRFLDLGQCNNSGSAVKIALALAEAF
HHHHCCCCEEEEEECCCCEEECCCCCEEECCCCCCCEEECCCCCCCCCEEEHHHHHHHHH
NCGVNDLPLSIVLSWFEQKAVAILLGLFSLGVKNMYIGPKAPEFLTPGVVEVLQKTFNLQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHCEE
LISGDANADLVKMLG
EEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ammonia; H2O; acceptor

Specific reaction: ammonia + H2O + acceptor = hydroxylamine + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA