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The map label for this gene is ptsI [H]

Identifier: 134298207

GI number: 134298207

Start: 343886

End: 345508

Strand: Reverse

Name: ptsI [H]

Synonym: Dred_0330

Alternate gene names: 134298207

Gene position: 345508-343886 (Counterclockwise)

Preceding gene: 134298208

Following gene: 134298205

Centisome position: 9.58

GC content: 46.46

Gene sequence:

>1623_bases
ATGTTACGGGGAATTGGGGTTTCCACTGGCCTAGGCATTGCCAAAGCAGTGATATATCAAAAAGAGGATTTGATTATTCC
TAAGGTTAAGTTAGTTGAAAATGATCTAGAAGCGGAAGTCAAAAAGTTTGAACAGGCCCAGGCTTCGGCAAAACAAGAAT
TGTTGCAAATTAAGGAAAGGGTTTCACAGACTGCCGGTAAAGAAAAGGCAGATATAATCGAAGCTCAAATCATGTTTTTA
GAAGACCCGACGGTGGTTAGCGAGATAATTAATATAATTAAAGCCGGTAACAATGCTGCCTATGCCGTAAATCAGGCGGT
TGAAGCACAGGCCCGGATGTTGGAAAGTCTGCCGGTGCCAGAAATGCAAGAGCGGGCTGTGGATATGCGGGATATGGGGC
ACAGGTTGATAAATATTTTACTTGATCGCAATGAGGTGAGTTTAGCCGAGTTAACCGAGCAGACTGTACTGATAGCCCAT
GACCTTACCCCTTCTGATACCGCTCAACTATCCAGGGAGCGGGTGGCGGCAATTGTTACCGAAGTGGGCAGTGGAACCAG
TCATACAGCAATTTTGGCTCGTTCCCTGGGTATCCCCGCAGTTGTGGGCATTCCTGGTATTGTAGAGCAAGTTAGTTCGG
GTGTTCTGGTAGCTGTAGATGGCAGCACCGGTGAAGTATTTATTGATCCTGACCAGGTTAAGTTAGGCAGCCTAAAACAA
AAATTGGAACAGTTTGTTGCTGAACAAAGAGACCTGCAAGGCTTGGTTAATGAGAAAGCGGTAACTGCTAACGGTATTCC
CGTCAGGCTGGCCGCCAATATTGGTAAACTAAGTGATATCATTGAAGCACAGAAAGTGGGCGTAAAGGAAATTGGTCTCT
TTAGGACAGAGTTTTTATATATGGACAGAAACTCTCTGCCCACCGAAGAGGAGCAGTACCAGTCCTATAAACAAGTAGTA
GAAGCTTTTCCCCACAGGGTTATCATCAGAACTCTAGATATCGGCGGAGATAAAGAGTTACCCTATCTAAAGCTAGCCAA
AGAACTTAACCCCTTTTTGGGTAAACGGGCAATACGACTAGCTCTGACGGAAAAGGGTTTATTTAAAACTCAGCTAAGAG
CCATTCTCCGGGCCAGCAGACACGGAAACATTGGCATAATGTTTCCCATGATTGCTACACTAGAGGAATTACAGGCGGCC
AAAGAACTACTGGCGGCAGCAAAGGCTGAACTGGTGGCTGAAGGAGTAGAGTTAGCCAATCCAGTGGAAGTGGGAATGAT
GATGGAAACCCCGGCAGCTGCCCTGGCAGCCGATATCTTTGCTAAAGAAGTTGATTTCTTCAGCATTGGCACCAATGATC
TAATCCAATACACTATGGCCGCCGATCGTATGAATGAACAGGTAGCCTATCTATACCAACCCACCCACCCGGCGATATGC
CGTCTGTTGGAGGGAATTGTCCAGGCAGGGCATAATGCAGGTATCTGGGTAGGTATGTGCGGTGAAATGGCCGGCAACCC
GGAATATACAGAGCACCTGCTGAAGATGGGATTAGATGAACTAAGCATGACCCGGGGAGCGGTACTGAGGGTAAAACAAA
AAATTCGCCAGATATCTTTATAG

Upstream 100 bases:

>100_bases
ACTTTTAGTAAGTGCGGAAGGAAATCAGGCAGAAGCAGCAGTAAATGCTCTGGCTAACCTGCTAAGTAAGCCCTTTACAG
AATAGAAAGGGGGTAGCAAT

Downstream 100 bases:

>100_bases
CAGTCAATTACTGATACCAGTAAGGACCTCCGTAATCAGGAGGTCCTCGCATTTTGGTCGGTTATAAATTATTTCCTAGT
TACTGGTATATTAAGTCCCT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MLRGIGVSTGLGIAKAVIYQKEDLIIPKVKLVENDLEAEVKKFEQAQASAKQELLQIKERVSQTAGKEKADIIEAQIMFL
EDPTVVSEIINIIKAGNNAAYAVNQAVEAQARMLESLPVPEMQERAVDMRDMGHRLINILLDRNEVSLAELTEQTVLIAH
DLTPSDTAQLSRERVAAIVTEVGSGTSHTAILARSLGIPAVVGIPGIVEQVSSGVLVAVDGSTGEVFIDPDQVKLGSLKQ
KLEQFVAEQRDLQGLVNEKAVTANGIPVRLAANIGKLSDIIEAQKVGVKEIGLFRTEFLYMDRNSLPTEEEQYQSYKQVV
EAFPHRVIIRTLDIGGDKELPYLKLAKELNPFLGKRAIRLALTEKGLFKTQLRAILRASRHGNIGIMFPMIATLEELQAA
KELLAAAKAELVAEGVELANPVEVGMMMETPAAALAADIFAKEVDFFSIGTNDLIQYTMAADRMNEQVAYLYQPTHPAIC
RLLEGIVQAGHNAGIWVGMCGEMAGNPEYTEHLLKMGLDELSMTRGAVLRVKQKIRQISL

Sequences:

>Translated_540_residues
MLRGIGVSTGLGIAKAVIYQKEDLIIPKVKLVENDLEAEVKKFEQAQASAKQELLQIKERVSQTAGKEKADIIEAQIMFL
EDPTVVSEIINIIKAGNNAAYAVNQAVEAQARMLESLPVPEMQERAVDMRDMGHRLINILLDRNEVSLAELTEQTVLIAH
DLTPSDTAQLSRERVAAIVTEVGSGTSHTAILARSLGIPAVVGIPGIVEQVSSGVLVAVDGSTGEVFIDPDQVKLGSLKQ
KLEQFVAEQRDLQGLVNEKAVTANGIPVRLAANIGKLSDIIEAQKVGVKEIGLFRTEFLYMDRNSLPTEEEQYQSYKQVV
EAFPHRVIIRTLDIGGDKELPYLKLAKELNPFLGKRAIRLALTEKGLFKTQLRAILRASRHGNIGIMFPMIATLEELQAA
KELLAAAKAELVAEGVELANPVEVGMMMETPAAALAADIFAKEVDFFSIGTNDLIQYTMAADRMNEQVAYLYQPTHPAIC
RLLEGIVQAGHNAGIWVGMCGEMAGNPEYTEHLLKMGLDELSMTRGAVLRVKQKIRQISL
>Mature_540_residues
MLRGIGVSTGLGIAKAVIYQKEDLIIPKVKLVENDLEAEVKKFEQAQASAKQELLQIKERVSQTAGKEKADIIEAQIMFL
EDPTVVSEIINIIKAGNNAAYAVNQAVEAQARMLESLPVPEMQERAVDMRDMGHRLINILLDRNEVSLAELTEQTVLIAH
DLTPSDTAQLSRERVAAIVTEVGSGTSHTAILARSLGIPAVVGIPGIVEQVSSGVLVAVDGSTGEVFIDPDQVKLGSLKQ
KLEQFVAEQRDLQGLVNEKAVTANGIPVRLAANIGKLSDIIEAQKVGVKEIGLFRTEFLYMDRNSLPTEEEQYQSYKQVV
EAFPHRVIIRTLDIGGDKELPYLKLAKELNPFLGKRAIRLALTEKGLFKTQLRAILRASRHGNIGIMFPMIATLEELQAA
KELLAAAKAELVAEGVELANPVEVGMMMETPAAALAADIFAKEVDFFSIGTNDLIQYTMAADRMNEQVAYLYQPTHPAIC
RLLEGIVQAGHNAGIWVGMCGEMAGNPEYTEHLLKMGLDELSMTRGAVLRVKQKIRQISL

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=542, Percent_Identity=43.1734317343173, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1788726, Length=550, Percent_Identity=35.0909090909091, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI48994992, Length=493, Percent_Identity=36.5111561866126, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1789193, Length=553, Percent_Identity=33.8155515370705, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1787994, Length=479, Percent_Identity=24.4258872651357, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59029; Mature: 59029

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGIGVSTGLGIAKAVIYQKEDLIIPKVKLVENDLEAEVKKFEQAQASAKQELLQIKER
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSQTAGKEKADIIEAQIMFLEDPTVVSEIINIIKAGNNAAYAVNQAVEAQARMLESLPVP
HHHHCCCHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCC
EMQERAVDMRDMGHRLINILLDRNEVSLAELTEQTVLIAHDLTPSDTAQLSRERVAAIVT
CHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHH
EVGSGTSHTAILARSLGIPAVVGIPGIVEQVSSGVLVAVDGSTGEVFIDPDQVKLGSLKQ
HHCCCCCHHHHHHHHCCCCEECCCCHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHH
KLEQFVAEQRDLQGLVNEKAVTANGIPVRLAANIGKLSDIIEAQKVGVKEIGLFRTEFLY
HHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHEEE
MDRNSLPTEEEQYQSYKQVVEAFPHRVIIRTLDIGGDKELPYLKLAKELNPFLGKRAIRL
ECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHEE
ALTEKGLFKTQLRAILRASRHGNIGIMFPMIATLEELQAAKELLAAAKAELVAEGVELAN
EHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PVEVGMMMETPAAALAADIFAKEVDFFSIGTNDLIQYTMAADRMNEQVAYLYQPTHPAIC
CHHHCEEECCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHH
RLLEGIVQAGHNAGIWVGMCGEMAGNPEYTEHLLKMGLDELSMTRGAVLRVKQKIRQISL
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRGIGVSTGLGIAKAVIYQKEDLIIPKVKLVENDLEAEVKKFEQAQASAKQELLQIKER
CCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSQTAGKEKADIIEAQIMFLEDPTVVSEIINIIKAGNNAAYAVNQAVEAQARMLESLPVP
HHHHCCCHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCC
EMQERAVDMRDMGHRLINILLDRNEVSLAELTEQTVLIAHDLTPSDTAQLSRERVAAIVT
CHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHH
EVGSGTSHTAILARSLGIPAVVGIPGIVEQVSSGVLVAVDGSTGEVFIDPDQVKLGSLKQ
HHCCCCCHHHHHHHHCCCCEECCCCHHHHHHCCCEEEEEECCCCEEEECCCCCHHHHHHH
KLEQFVAEQRDLQGLVNEKAVTANGIPVRLAANIGKLSDIIEAQKVGVKEIGLFRTEFLY
HHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHEEE
MDRNSLPTEEEQYQSYKQVVEAFPHRVIIRTLDIGGDKELPYLKLAKELNPFLGKRAIRL
ECCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHCHHHHHHHHEE
ALTEKGLFKTQLRAILRASRHGNIGIMFPMIATLEELQAAKELLAAAKAELVAEGVELAN
EHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PVEVGMMMETPAAALAADIFAKEVDFFSIGTNDLIQYTMAADRMNEQVAYLYQPTHPAIC
CHHHCEEECCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHH
RLLEGIVQAGHNAGIWVGMCGEMAGNPEYTEHLLKMGLDELSMTRGAVLRVKQKIRQISL
HHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA